ID   GA45A_MOUSE             Reviewed;         165 AA.
AC   P48316;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   24-JAN-2024, entry version 154.
DE   RecName: Full=Growth arrest and DNA damage-inducible protein GADD45 alpha;
DE   AltName: Full=DNA damage-inducible transcript 1 protein;
DE            Short=DDIT-1;
GN   Name=Gadd45a; Synonyms=Ddit1, Gadd45;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=129/Sv; TISSUE=Liver;
RX   PubMed=8139541; DOI=10.1128/mcb.14.4.2361-2371.1994;
RA   Zhan Q., Lord K.A., Alamo I. Jr., Hollander M.C., Carrier F., Ron D.,
RA   Kohn K.W., Hoffman B., Liebermann D.A., Fornace A.J. Jr.;
RT   "The gadd and MyD genes define a novel set of mammalian genes encoding
RT   acidic proteins that synergistically suppress cell growth.";
RL   Mol. Cell. Biol. 14:2361-2371(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=129/Sv; TISSUE=Liver;
RX   PubMed=7509226;
RA   Alimzhanov M.B., Kuprash D.V., Turetskaya R.L., Osipovich O.A.,
RA   Borodulina O.R., Osovskaia V.S., Chumakov R.M., Nedospasov S.A.;
RT   "Cloning and characteristics of murine genes coding for the human GADD45
RT   analog -- a protein induced in response to DNA damage.";
RL   Dokl. Akad. Nauk 333:788-791(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Mammary gland;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   INTERACTION WITH MAPK14, AND FUNCTION.
RX   PubMed=15735649; DOI=10.1038/ni1176;
RA   Salvador J.M., Mittelstadt P.R., Belova G.I., Fornace A.J. Jr.,
RA   Ashwell J.D.;
RT   "The autoimmune suppressor Gadd45alpha inhibits the T cell alternative p38
RT   activation pathway.";
RL   Nat. Immunol. 6:396-402(2005).
CC   -!- FUNCTION: Might affect PCNA interaction with some CDK (cell division
CC       protein kinase) complexes; stimulates DNA excision repair in vitro and
CC       inhibits entry of cells into S phase. In T-cells, functions as a
CC       regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity.
CC       {ECO:0000269|PubMed:15735649}.
CC   -!- SUBUNIT: Interacts with AURKA, GADD45GIP1 and PCNA (By similarity).
CC       Interacts with MAPK14. {ECO:0000250, ECO:0000269|PubMed:15735649}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- INDUCTION: By various types of DNA damage and growth arrest.
CC   -!- SIMILARITY: Belongs to the GADD45 family. {ECO:0000305}.
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DR   EMBL; L28177; AAA37656.1; -; mRNA.
DR   EMBL; U00937; AAC27351.1; -; Unassigned_DNA.
DR   EMBL; BC011141; AAH11141.1; -; mRNA.
DR   CCDS; CCDS20217.1; -.
DR   PIR; B56535; B56535.
DR   RefSeq; NP_031862.1; NM_007836.1.
DR   AlphaFoldDB; P48316; -.
DR   SMR; P48316; -.
DR   BioGRID; 199077; 6.
DR   IntAct; P48316; 1.
DR   STRING; 10090.ENSMUSP00000044034; -.
DR   PhosphoSitePlus; P48316; -.
DR   PaxDb; 10090-ENSMUSP00000044034; -.
DR   ProteomicsDB; 265726; -.
DR   Antibodypedia; 33411; 490 antibodies from 34 providers.
DR   DNASU; 13197; -.
DR   Ensembl; ENSMUST00000043098.9; ENSMUSP00000044034.7; ENSMUSG00000036390.9.
DR   GeneID; 13197; -.
DR   KEGG; mmu:13197; -.
DR   UCSC; uc009cfc.1; mouse.
DR   AGR; MGI:107799; -.
DR   CTD; 1647; -.
DR   MGI; MGI:107799; Gadd45a.
DR   VEuPathDB; HostDB:ENSMUSG00000036390; -.
DR   eggNOG; ENOG502RY8P; Eukaryota.
DR   GeneTree; ENSGT00950000182964; -.
DR   HOGENOM; CLU_118164_0_0_1; -.
DR   InParanoid; P48316; -.
DR   OMA; CLLVDFP; -.
DR   OrthoDB; 4103218at2759; -.
DR   PhylomeDB; P48316; -.
DR   TreeFam; TF300196; -.
DR   Reactome; R-MMU-6804114; TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
DR   BioGRID-ORCS; 13197; 8 hits in 79 CRISPR screens.
DR   PRO; PR:P48316; -.
DR   Proteomes; UP000000589; Chromosome 6.
DR   RNAct; P48316; Protein.
DR   Bgee; ENSMUSG00000036390; Expressed in granulocyte and 264 other cell types or tissues.
DR   ExpressionAtlas; P48316; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR   GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0019900; F:kinase binding; ISO:MGI.
DR   GO; GO:1990841; F:promoter-specific chromatin binding; IDA:MGI.
DR   GO; GO:0046982; F:protein heterodimerization activity; ISO:MGI.
DR   GO; GO:0042803; F:protein homodimerization activity; ISO:MGI.
DR   GO; GO:0071479; P:cellular response to ionizing radiation; ISO:MGI.
DR   GO; GO:0071260; P:cellular response to mechanical stimulus; IEA:Ensembl.
DR   GO; GO:0007098; P:centrosome cycle; IGI:MGI.
DR   GO; GO:0016525; P:negative regulation of angiogenesis; IMP:CACAO.
DR   GO; GO:0043537; P:negative regulation of blood vessel endothelial cell migration; IMP:CACAO.
DR   GO; GO:0033140; P:negative regulation of peptidyl-serine phosphorylation of STAT protein; IMP:CACAO.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR   GO; GO:0043065; P:positive regulation of apoptotic process; ISO:MGI.
DR   GO; GO:0046330; P:positive regulation of JNK cascade; ISO:MGI.
DR   GO; GO:1900745; P:positive regulation of p38MAPK cascade; ISO:MGI.
DR   GO; GO:2000379; P:positive regulation of reactive oxygen species metabolic process; ISO:MGI.
DR   GO; GO:0051726; P:regulation of cell cycle; IDA:MGI.
DR   GO; GO:0042770; P:signal transduction in response to DNA damage; ISO:MGI.
DR   Gene3D; 3.30.1330.30; -; 1.
DR   InterPro; IPR024824; GADD45.
DR   InterPro; IPR029064; Ribosomal_eL30-like_sf.
DR   InterPro; IPR004038; Ribosomal_eL8/eL30/eS12/Gad45.
DR   PANTHER; PTHR10411; GROWTH ARREST AND DNA DAMAGE-INDUCIBLE PROTEIN GADD45; 1.
DR   PANTHER; PTHR10411:SF3; GROWTH ARREST AND DNA DAMAGE-INDUCIBLE PROTEIN GADD45 ALPHA; 1.
DR   Pfam; PF01248; Ribosomal_L7Ae; 1.
DR   SUPFAM; SSF55315; L30e-like; 1.
DR   Genevisible; P48316; MM.
PE   1: Evidence at protein level;
KW   Cell cycle; DNA damage; Growth arrest; Nucleus; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN           1..165
FT                   /note="Growth arrest and DNA damage-inducible protein
FT                   GADD45 alpha"
FT                   /id="PRO_0000148332"
FT   MOD_RES         2
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:P24522"
SQ   SEQUENCE   165 AA;  18339 MW;  C96BAB354A84770F CRC64;
     MTLEEFSAAE QKTERMDTVG DALEEVLSKA RSQRTITVGV YEAAKLLNVD PDNVVLCLLA
     ADEDDDRDVA LQIHFTLIRA FCCENDINIL RVSNPGRLAE LLLLENDAGP AESGGAAQTP
     DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPER
//