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P48316 (GA45A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Growth arrest and DNA damage-inducible protein GADD45 alpha
Alternative name(s):
DNA damage-inducible transcript 1 protein
Short name=DDIT-1
Gene names
Name:Gadd45a
Synonyms:Ddit1, Gadd45
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length165 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Ref.4

Subunit structure

Interacts with AURKA, GADD45GIP1 and PCNA By similarity. Interacts with MAPK14. Ref.4

Subcellular location

Nucleus By similarity.

Induction

By various types of DNA damage and growth arrest.

Sequence similarities

Belongs to the GADD45 family.

Ontologies

Keywords
   Biological processCell cycle
DNA damage
Growth arrest
   Cellular componentNucleus
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of MAPKKK activity

Inferred from electronic annotation. Source: Ensembl

cell cycle arrest

Inferred from electronic annotation. Source: UniProtKB-KW

cellular response to ionizing radiation

Inferred from electronic annotation. Source: Ensembl

cellular response to mechanical stimulus

Inferred from electronic annotation. Source: Ensembl

centrosome cycle

Inferred from genetic interaction PubMed 15123655. Source: MGI

negative regulation of protein kinase activity

Inferred from direct assay PubMed 12124778. Source: MGI

positive regulation of JNK cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of p38MAPK cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of reactive oxygen species metabolic process

Inferred from electronic annotation. Source: Ensembl

regulation of cell cycle

Inferred from direct assay PubMed 12124778. Source: MGI

signal transduction in response to DNA damage

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 19796622. Source: MGI

   Molecular_functioncore promoter binding

Inferred from direct assay PubMed 22323595. Source: MGI

protein binding

Inferred from physical interaction PubMed 12124778. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 165165Growth arrest and DNA damage-inducible protein GADD45 alpha
PRO_0000148332

Regions

Region1 – 1616Intrinsically disordered By similarity
Region105 – 11814Intrinsically disordered By similarity

Sequences

Sequence LengthMass (Da)Tools
P48316 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: C96BAB354A84770F

FASTA16518,339
        10         20         30         40         50         60 
MTLEEFSAAE QKTERMDTVG DALEEVLSKA RSQRTITVGV YEAAKLLNVD PDNVVLCLLA 

        70         80         90        100        110        120 
ADEDDDRDVA LQIHFTLIRA FCCENDINIL RVSNPGRLAE LLLLENDAGP AESGGAAQTP 

       130        140        150        160 
DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPER 

« Hide

References

« Hide 'large scale' references
[1]"The gadd and MyD genes define a novel set of mammalian genes encoding acidic proteins that synergistically suppress cell growth."
Zhan Q., Lord K.A., Alamo I. Jr., Hollander M.C., Carrier F., Ron D., Kohn K.W., Hoffman B., Liebermann D.A., Fornace A.J. Jr.
Mol. Cell. Biol. 14:2361-2371(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 129/Sv.
Tissue: Liver.
[2]"Cloning and characteristics of murine genes coding for the human GADD45 analog -- a protein induced in response to DNA damage."
Alimzhanov M.B., Kuprash D.V., Turetskaya R.L., Osipovich O.A., Borodulina O.R., Osovskaia V.S., Chumakov R.M., Nedospasov S.A.
Dokl. Akad. Nauk 333:788-791(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 129/Sv.
Tissue: Liver.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Mammary gland.
[4]"The autoimmune suppressor Gadd45alpha inhibits the T cell alternative p38 activation pathway."
Salvador J.M., Mittelstadt P.R., Belova G.I., Fornace A.J. Jr., Ashwell J.D.
Nat. Immunol. 6:396-402(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MAPK14, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L28177 mRNA. Translation: AAA37656.1.
U00937 Unassigned DNA. Translation: AAC27351.1.
BC011141 mRNA. Translation: AAH11141.1.
CCDSCCDS20217.1.
PIRB56535.
RefSeqNP_031862.1. NM_007836.1.
UniGeneMm.72235.

3D structure databases

ProteinModelPortalP48316.
SMRP48316. Positions 1-165.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199077. 2 interactions.
IntActP48316. 1 interaction.
STRING10090.ENSMUSP00000044034.

PTM databases

PhosphoSiteP48316.

Proteomic databases

PRIDEP48316.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000043098; ENSMUSP00000044034; ENSMUSG00000036390.
GeneID13197.
KEGGmmu:13197.
UCSCuc009cfc.1. mouse.

Organism-specific databases

CTD1647.
MGIMGI:107799. Gadd45a.

Phylogenomic databases

eggNOGNOG75953.
HOGENOMHOG000013221.
HOVERGENHBG051684.
InParanoidP48316.
KOK04402.
OMAHCVLVTS.
OrthoDBEOG7J1819.
PhylomeDBP48316.
TreeFamTF300196.

Gene expression databases

ArrayExpressP48316.
BgeeP48316.
CleanExMM_GADD45A.
GenevestigatorP48316.

Family and domain databases

InterProIPR024824. GADD45.
IPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
[Graphical view]
PANTHERPTHR10411. PTHR10411. 1 hit.
PfamPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
SUPFAMSSF55315. SSF55315. 1 hit.
ProtoNetSearch...

Other

NextBio283332.
PROP48316.
SOURCESearch...

Entry information

Entry nameGA45A_MOUSE
AccessionPrimary (citable) accession number: P48316
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot