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Protein

Growth arrest and DNA damage-inducible protein GADD45 alpha

Gene

Gadd45a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity.1 Publication

GO - Molecular functioni

  1. core promoter binding Source: MGI

GO - Biological processi

  1. activation of MAPKKK activity Source: MGI
  2. cell cycle arrest Source: UniProtKB-KW
  3. cellular response to ionizing radiation Source: MGI
  4. cellular response to mechanical stimulus Source: Ensembl
  5. centrosome cycle Source: MGI
  6. negative regulation of protein kinase activity Source: MGI
  7. positive regulation of apoptotic process Source: MGI
  8. positive regulation of JNK cascade Source: MGI
  9. positive regulation of p38MAPK cascade Source: MGI
  10. positive regulation of reactive oxygen species metabolic process Source: MGI
  11. regulation of cell cycle Source: MGI
  12. signal transduction in response to DNA damage Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA damage, Growth arrest

Names & Taxonomyi

Protein namesi
Recommended name:
Growth arrest and DNA damage-inducible protein GADD45 alpha
Alternative name(s):
DNA damage-inducible transcript 1 protein
Short name:
DDIT-1
Gene namesi
Name:Gadd45a
Synonyms:Ddit1, Gadd45
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:107799. Gadd45a.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 165165Growth arrest and DNA damage-inducible protein GADD45 alphaPRO_0000148332Add
BLAST

Proteomic databases

PRIDEiP48316.

PTM databases

PhosphoSiteiP48316.

Expressioni

Inductioni

By various types of DNA damage and growth arrest.

Gene expression databases

BgeeiP48316.
CleanExiMM_GADD45A.
ExpressionAtlasiP48316. baseline and differential.
GenevestigatoriP48316.

Interactioni

Subunit structurei

Interacts with AURKA, GADD45GIP1 and PCNA (By similarity). Interacts with MAPK14.By similarity1 Publication

Protein-protein interaction databases

BioGridi199077. 4 interactions.
IntActiP48316. 1 interaction.
STRINGi10090.ENSMUSP00000044034.

Structurei

3D structure databases

ProteinModelPortaliP48316.
SMRiP48316. Positions 1-165.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 1616Intrinsically disorderedBy similarityAdd
BLAST
Regioni105 – 11814Intrinsically disorderedBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the GADD45 family.Curated

Phylogenomic databases

eggNOGiNOG75953.
HOGENOMiHOG000013221.
HOVERGENiHBG051684.
InParanoidiP48316.
KOiK04402.
OMAiNPHASQW.
OrthoDBiEOG7J1819.
PhylomeDBiP48316.
TreeFamiTF300196.

Family and domain databases

InterProiIPR024824. GADD45.
IPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
[Graphical view]
PANTHERiPTHR10411. PTHR10411. 1 hit.
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
SUPFAMiSSF55315. SSF55315. 1 hit.

Sequencei

Sequence statusi: Complete.

P48316-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLEEFSAAE QKTERMDTVG DALEEVLSKA RSQRTITVGV YEAAKLLNVD
60 70 80 90 100
PDNVVLCLLA ADEDDDRDVA LQIHFTLIRA FCCENDINIL RVSNPGRLAE
110 120 130 140 150
LLLLENDAGP AESGGAAQTP DLHCVLVTNP HSSQWKDPAL SQLICFCRES
160
RYMDQWVPVI NLPER
Length:165
Mass (Da):18,339
Last modified:February 1, 1996 - v1
Checksum:iC96BAB354A84770F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28177 mRNA. Translation: AAA37656.1.
U00937 Unassigned DNA. Translation: AAC27351.1.
BC011141 mRNA. Translation: AAH11141.1.
CCDSiCCDS20217.1.
PIRiB56535.
RefSeqiNP_031862.1. NM_007836.1.
UniGeneiMm.72235.

Genome annotation databases

EnsembliENSMUST00000043098; ENSMUSP00000044034; ENSMUSG00000036390.
GeneIDi13197.
KEGGimmu:13197.
UCSCiuc009cfc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28177 mRNA. Translation: AAA37656.1.
U00937 Unassigned DNA. Translation: AAC27351.1.
BC011141 mRNA. Translation: AAH11141.1.
CCDSiCCDS20217.1.
PIRiB56535.
RefSeqiNP_031862.1. NM_007836.1.
UniGeneiMm.72235.

3D structure databases

ProteinModelPortaliP48316.
SMRiP48316. Positions 1-165.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199077. 4 interactions.
IntActiP48316. 1 interaction.
STRINGi10090.ENSMUSP00000044034.

PTM databases

PhosphoSiteiP48316.

Proteomic databases

PRIDEiP48316.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043098; ENSMUSP00000044034; ENSMUSG00000036390.
GeneIDi13197.
KEGGimmu:13197.
UCSCiuc009cfc.1. mouse.

Organism-specific databases

CTDi1647.
MGIiMGI:107799. Gadd45a.

Phylogenomic databases

eggNOGiNOG75953.
HOGENOMiHOG000013221.
HOVERGENiHBG051684.
InParanoidiP48316.
KOiK04402.
OMAiNPHASQW.
OrthoDBiEOG7J1819.
PhylomeDBiP48316.
TreeFamiTF300196.

Miscellaneous databases

NextBioi283332.
PROiP48316.
SOURCEiSearch...

Gene expression databases

BgeeiP48316.
CleanExiMM_GADD45A.
ExpressionAtlasiP48316. baseline and differential.
GenevestigatoriP48316.

Family and domain databases

InterProiIPR024824. GADD45.
IPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
[Graphical view]
PANTHERiPTHR10411. PTHR10411. 1 hit.
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
SUPFAMiSSF55315. SSF55315. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The gadd and MyD genes define a novel set of mammalian genes encoding acidic proteins that synergistically suppress cell growth."
    Zhan Q., Lord K.A., Alamo I. Jr., Hollander M.C., Carrier F., Ron D., Kohn K.W., Hoffman B., Liebermann D.A., Fornace A.J. Jr.
    Mol. Cell. Biol. 14:2361-2371(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129/Sv.
    Tissue: Liver.
  2. "Cloning and characteristics of murine genes coding for the human GADD45 analog -- a protein induced in response to DNA damage."
    Alimzhanov M.B., Kuprash D.V., Turetskaya R.L., Osipovich O.A., Borodulina O.R., Osovskaia V.S., Chumakov R.M., Nedospasov S.A.
    Dokl. Akad. Nauk 333:788-791(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 129/Sv.
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  4. "The autoimmune suppressor Gadd45alpha inhibits the T cell alternative p38 activation pathway."
    Salvador J.M., Mittelstadt P.R., Belova G.I., Fornace A.J. Jr., Ashwell J.D.
    Nat. Immunol. 6:396-402(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAPK14, FUNCTION.

Entry informationi

Entry nameiGA45A_MOUSE
AccessioniPrimary (citable) accession number: P48316
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 4, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.