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Protein

Tissue factor pathway inhibitor 2

Gene

TFPI2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei46 – 47Reactive bondBy similarity2
Sitei107 – 108Reactive bondBy similarity2
Sitei168 – 169Reactive bondBy similarity2

GO - Molecular functioni

  • extracellular matrix structural constituent Source: ProtInc
  • serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor
Biological processBlood coagulation, Hemostasis

Protein family/group databases

MEROPSiI02.951.

Names & Taxonomyi

Protein namesi
Recommended name:
Tissue factor pathway inhibitor 2
Short name:
TFPI-2
Alternative name(s):
Placental protein 5
Short name:
PP5
Gene namesi
Name:TFPI2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:11761. TFPI2.

Subcellular locationi

GO - Cellular componenti

  • extracellular matrix Source: BHF-UCL
  • proteinaceous extracellular matrix Source: ProtInc

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi7980.
OpenTargetsiENSG00000105825.
PharmGKBiPA36476.

Polymorphism and mutation databases

DMDMi1351226.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 222 PublicationsAdd BLAST22
ChainiPRO_000001687623 – 235Tissue factor pathway inhibitor 2Add BLAST213

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 86PROSITE-ProRule annotation1 Publication
Disulfide bondi45 ↔ 69PROSITE-ProRule annotation1 Publication
Disulfide bondi61 ↔ 82PROSITE-ProRule annotation1 Publication
Disulfide bondi96 ↔ 149PROSITE-ProRule annotation
Disulfide bondi106 ↔ 130PROSITE-ProRule annotation
Glycosylationi116N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi122 ↔ 145PROSITE-ProRule annotation
Disulfide bondi158 ↔ 208PROSITE-ProRule annotation
Disulfide bondi167 ↔ 191PROSITE-ProRule annotation
Glycosylationi170N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi183 ↔ 204PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP48307.
PaxDbiP48307.
PeptideAtlasiP48307.
PRIDEiP48307.

PTM databases

iPTMnetiP48307.
PhosphoSitePlusiP48307.

Miscellaneous databases

PMAP-CutDBiP48307.

Expressioni

Tissue specificityi

Umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy.

Gene expression databases

BgeeiENSG00000105825.
CleanExiHS_TFPI2.
ExpressionAtlasiP48307. baseline and differential.
GenevisibleiP48307. HS.

Organism-specific databases

HPAiCAB010142.
HPA049158.

Interactioni

Subunit structurei

Finds in a complex with ABCB1, TFPI2 and PPP2R3C; leading to the dephosphorylation of ABCB1.1 Publication

Protein-protein interaction databases

BioGridi113693. 2 interactors.
IntActiP48307. 2 interactors.
MINTiMINT-1187323.
STRINGi9606.ENSP00000222543.

Structurei

Secondary structure

1235
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi33 – 36Combined sources4
Beta strandi49 – 55Combined sources7
Turni56 – 59Combined sources4
Beta strandi60 – 66Combined sources7
Beta strandi76 – 78Combined sources3
Helixi79 – 85Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZR0X-ray1.80B/D28-90[»]
ProteinModelPortaliP48307.
SMRiP48307.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP48307.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 86BPTI/Kunitz inhibitor 1PROSITE-ProRule annotationAdd BLAST51
Domaini96 – 149BPTI/Kunitz inhibitor 2PROSITE-ProRule annotationAdd BLAST54
Domaini158 – 208BPTI/Kunitz inhibitor 3PROSITE-ProRule annotationAdd BLAST51

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi213 – 217Poly-Lys5

Domaini

This inhibitor contains three inhibitory domains.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG4295. Eukaryota.
ENOG410XQNP. LUCA.
GeneTreeiENSGT00740000114929.
HOGENOMiHOG000231818.
HOVERGENiHBG056804.
InParanoidiP48307.
OMAiDRYTQSC.
OrthoDBiEOG091G14M8.
PhylomeDBiP48307.
TreeFamiTF315349.

Family and domain databases

Gene3Di4.10.410.10. 3 hits.
InterProiView protein in InterPro
IPR002223. Kunitz_BPTI.
IPR020901. Prtase_inh_Kunz-CS.
PfamiView protein in Pfam
PF00014. Kunitz_BPTI. 3 hits.
PRINTSiPR00759. BASICPTASE.
SMARTiView protein in SMART
SM00131. KU. 3 hits.
SUPFAMiSSF57362. SSF57362. 3 hits.
PROSITEiView protein in PROSITE
PS00280. BPTI_KUNITZ_1. 2 hits.
PS50279. BPTI_KUNITZ_2. 3 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P48307-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPARPLGLS ILLLFLTEAA LGDAAQEPTG NNAEICLLPL DYGPCRALLL
60 70 80 90 100
RYYYDRYTQS CRQFLYGGCE GNANNFYTWE ACDDACWRIE KVPKVCRLQV
110 120 130 140 150
SVDDQCEGST EKYFFNLSSM TCEKFFSGGC HRNRIENRFP DEATCMGFCA
160 170 180 190 200
PKKIPSFCYS PKDEGLCSAN VTRYYFNPRY RTCDAFTYTG CGGNDNNFVS
210 220 230
REDCKRACAK ALKKKKKMPK LRFASRIRKI RKKQF
Length:235
Mass (Da):26,934
Last modified:February 1, 1996 - v1
Checksum:i975ABA5C53F7C65F
GO
Isoform 2 (identifier: P48307-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     30-40: Missing.

Note: No experimental confirmation available.
Show »
Length:224
Mass (Da):25,796
Checksum:iF586328C31344765
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23D → A AA sequence (PubMed:3276312).Curated1
Sequence conflicti23D → G AA sequence (PubMed:7872799).Curated1
Sequence conflicti36C → I AA sequence (PubMed:7872799).Curated1
Sequence conflicti42Y → R AA sequence (PubMed:7872799).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_012005102V → A. Corresponds to variant dbSNP:rs1804202Ensembl.1
Natural variantiVAR_050064231R → Q. Corresponds to variant dbSNP:rs12669450Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05603130 – 40Missing in isoform 2. 1 PublicationAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D29992 mRNA. Translation: BAA06272.1.
L27624 mRNA. Translation: AAA20094.1.
AF217542 Genomic DNA. Translation: AAK13254.1.
AY691946 mRNA. Translation: AAU04568.1.
AK092499 mRNA. Translation: BAC03906.1.
AK313260 mRNA. Translation: BAG36070.1.
AC002076 Genomic DNA. Translation: AAS02022.1.
CH236949 Genomic DNA. Translation: EAL24140.1.
CH471091 Genomic DNA. Translation: EAW76809.1.
CH471091 Genomic DNA. Translation: EAW76811.1.
BC005330 mRNA. Translation: AAH05330.1.
CCDSiCCDS5632.1. [P48307-1]
PIRiA54951.
S71593.
RefSeqiNP_001257932.1. NM_001271003.1. [P48307-2]
NP_006519.1. NM_006528.3. [P48307-1]
UniGeneiHs.438231.

Genome annotation databases

EnsembliENST00000222543; ENSP00000222543; ENSG00000105825. [P48307-1]
GeneIDi7980.
KEGGihsa:7980.
UCSCiuc003umy.2. human. [P48307-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTFPI2_HUMAN
AccessioniPrimary (citable) accession number: P48307
Secondary accession number(s): Q66ME8, Q8NAK6, Q9UC86
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 10, 2017
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references