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Protein

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

Gene

NDUFB4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

GO - Molecular functioni

GO - Biological processi

  • response to oxidative stress Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
Alternative name(s):
Complex I-B15
Short name:
CI-B15
NADH-ubiquinone oxidoreductase B15 subunit
Gene namesi
Name:NDUFB4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei86 – 10520HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 129128NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4PRO_0000118799Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine
Modified residuei26 – 261PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP48305.
PRIDEiP48305.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits.

Protein-protein interaction databases

IntActiP48305. 2 interactions.
STRINGi9913.ENSBTAP00000021123.

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I NDUFB4 subunit family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IZUV. Eukaryota.
ENOG4112CXK. LUCA.
HOGENOMiHOG000060241.
HOVERGENiHBG001159.
InParanoidiP48305.
KOiK03960.

Family and domain databases

InterProiIPR009866. NADH_UbQ_OxRdtase_NDUFB4_su.
[Graphical view]
PANTHERiPTHR15469. PTHR15469. 1 hit.
PfamiPF07225. NDUF_B4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48305-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFPKYEASR LSSLPTTLDP AEYDISSETR KAQAERLAIR SRLKREYQLQ
60 70 80 90 100
YYDPSRRGVI EDPALVRWTY ARSANIYPNF RPNTKTSLLG ALFGIGPLVF
110 120
WYYVFKTDRD RKEKLIQEGK LDRTFNISY
Length:129
Mass (Da):15,184
Last modified:January 23, 2007 - v2
Checksum:iF5DA3419E80D9125
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64898 mRNA. Translation: CAA46107.1.
BC103187 mRNA. Translation: AAI03188.1.
PIRiS28237.
RefSeqiNP_787016.1. NM_175822.2.
UniGeneiBt.49223.
Bt.60768.

Genome annotation databases

GeneIDi327706.
KEGGibta:327706.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64898 mRNA. Translation: CAA46107.1.
BC103187 mRNA. Translation: AAI03188.1.
PIRiS28237.
RefSeqiNP_787016.1. NM_175822.2.
UniGeneiBt.49223.
Bt.60768.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP48305. 2 interactions.
STRINGi9913.ENSBTAP00000021123.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP48305.
PRIDEiP48305.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi327706.
KEGGibta:327706.

Organism-specific databases

CTDi4710.

Phylogenomic databases

eggNOGiENOG410IZUV. Eukaryota.
ENOG4112CXK. LUCA.
HOGENOMiHOG000060241.
HOVERGENiHBG001159.
InParanoidiP48305.
KOiK03960.

Family and domain databases

InterProiIPR009866. NADH_UbQ_OxRdtase_NDUFB4_su.
[Graphical view]
PANTHERiPTHR15469. PTHR15469. 1 hit.
PfamiPF07225. NDUF_B4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Application of a novel strategy for sequencing proteins using the polymerase chain reaction."
    Walker J.E., Arizmendi J.M., Dupuis A., Fearnley I.M., Finel M., Medd S.M., Pilkington S.J., Runswick M.J., Skehel J.M.
    J. Mol. Biol. 226:1051-1072(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Heart.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Rumen.
  3. "Resolution of the membrane domain of bovine complex I into subcomplexes: implications for the structural organization of the enzyme."
    Sazanov L.A., Peak-Chew S.Y., Fearnley I.M., Walker J.E.
    Biochemistry 39:7229-7235(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, SUBUNIT, IDENTIFICATION IN COMPLEX I.
  4. "Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry."
    Lemma-Gray P., Valusova E., Carroll C.A., Weintraub S.T., Musatov A., Robinson N.C.
    Anal. Biochem. 382:116-121(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, IDENTIFICATION IN COMPLEX I.

Entry informationi

Entry nameiNDUB4_BOVIN
AccessioniPrimary (citable) accession number: P48305
Secondary accession number(s): Q54A31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.