Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P48303

- S1PR1_RAT

UniProt

P48303 - S1PR1_RAT

Protein

Sphingosine 1-phosphate receptor 1

Gene

S1pr1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate. Required for normal embryonic heart development and normal cardiac morphogenesis. Plays an important role in the regulation of sprouting angiogenesis and vascular maturation. Inhibits sprouting angiogenesis to prevent excessive sprouting during blood vessel development. Required for normal egress of mature T-cells from the thymus into the blood stream and into peripheral lymphoid organs. Plays a role in the migration of osteoclast precursor cells, the regulation of bone mineralization and bone homeostasis. Plays a role in responses to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine by pulmonary endothelial cells and in the protection against ventilator-induced lung injury By similarity.By similarity

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: RGD
    2. sphingolipid binding Source: RGD
    3. sphingosine-1-phosphate receptor activity Source: UniProtKB

    GO - Biological processi

    1. actin cytoskeleton reorganization Source: UniProtKB
    2. adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: RGD
    3. angiogenesis Source: UniProtKB-KW
    4. blood vessel maturation Source: UniProtKB
    5. brain development Source: Ensembl
    6. cardiac muscle tissue growth involved in heart morphogenesis Source: UniProtKB
    7. cell migration Source: UniProtKB
    8. chemotaxis Source: UniProtKB
    9. endothelial cell differentiation Source: RGD
    10. G-protein coupled receptor signaling pathway Source: RGD
    11. heart trabecula morphogenesis Source: UniProtKB
    12. lamellipodium assembly Source: UniProtKB
    13. negative regulation of stress fiber assembly Source: RGD
    14. neuron differentiation Source: RGD
    15. positive regulation of cell migration Source: RGD
    16. positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: RGD
    17. positive regulation of positive chemotaxis Source: RGD
    18. positive regulation of Ras GTPase activity Source: RGD
    19. positive regulation of smooth muscle cell proliferation Source: RGD
    20. positive regulation of transcription from RNA polymerase II promoter Source: RGD
    21. regulation of bone mineralization Source: UniProtKB
    22. regulation of bone resorption Source: UniProtKB
    23. regulation of cell adhesion Source: Ensembl
    24. sphingosine-1-phosphate signaling pathway Source: UniProtKB
    25. T cell migration Source: UniProtKB
    26. transmission of nerve impulse Source: RGD

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Angiogenesis, Chemotaxis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sphingosine 1-phosphate receptor 1
    Short name:
    S1P receptor 1
    Short name:
    S1P1
    Alternative name(s):
    Endothelial differentiation G-protein coupled receptor 1
    Sphingosine 1-phosphate receptor Edg-1
    Short name:
    S1P receptor Edg-1
    CD_antigen: CD363
    Gene namesi
    Name:S1pr1
    Synonyms:Edg1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi61958. S1pr1.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity. Endosome By similarity. Membrane raft By similarity
    Note: Recruited to caveolin-enriched plasma membrane microdomains in response to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine. Ligand binding leads to receptor internalization By similarity.By similarity

    GO - Cellular componenti

    1. endosome Source: UniProtKB-SubCell
    2. external side of plasma membrane Source: Ensembl
    3. integral component of membrane Source: UniProtKB-KW
    4. intrinsic component of plasma membrane Source: UniProtKB
    5. membrane raft Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 383383Sphingosine 1-phosphate receptor 1PRO_0000069414Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei11 – 111N6-acetyllysineBy similarity
    Glycosylationi31 – 311N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi185 ↔ 192PROSITE-ProRule annotation
    Modified residuei237 – 2371PhosphothreonineBy similarity
    Disulfide bondi283 ↔ 288PROSITE-ProRule annotation
    Lipidationi329 – 3291S-palmitoyl cysteineBy similarity
    Modified residuei354 – 3541PhosphoserineSequence Analysis

    Keywords - PTMi

    Acetylation, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PRIDEiP48303.

    PTM databases

    PhosphoSiteiP48303.

    Expressioni

    Developmental stagei

    First detected at embryonic day 15. At postnatal day 14 detected in skin, spleen, liver, kidney, heart, testicle, lung and brain. At adulthood is most abundant in brain.1 Publication

    Gene expression databases

    GenevestigatoriP48303.

    Interactioni

    Subunit structurei

    Interacts with GNAI1 and GNAI3.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000052627.

    Structurei

    3D structure databases

    ProteinModelPortaliP48303.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4747ExtracellularBy similarityAdd
    BLAST
    Topological domaini70 – 8314CytoplasmicBy similarityAdd
    BLAST
    Topological domaini106 – 11712ExtracellularBy similarityAdd
    BLAST
    Topological domaini140 – 16122CytoplasmicBy similarityAdd
    BLAST
    Topological domaini184 – 19714ExtracellularBy similarityAdd
    BLAST
    Topological domaini226 – 25833CytoplasmicBy similarityAdd
    BLAST
    Topological domaini280 – 29011ExtracellularBy similarityAdd
    BLAST
    Topological domaini312 – 38372CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei48 – 6922Helical; Name=1By similarityAdd
    BLAST
    Transmembranei84 – 10522Helical; Name=2By similarityAdd
    BLAST
    Transmembranei118 – 13922Helical; Name=3By similarityAdd
    BLAST
    Transmembranei162 – 18322Helical; Name=4By similarityAdd
    BLAST
    Transmembranei198 – 22528Helical; Name=5By similarityAdd
    BLAST
    Transmembranei259 – 27921Helical; Name=6By similarityAdd
    BLAST
    Transmembranei291 – 31121Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni121 – 1222Sphingosine 1-phosphate bindingBy similarity
    Regioni266 – 2705Sphingosine 1-phosphate bindingBy similarity

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG148018.
    GeneTreeiENSGT00750000117544.
    HOGENOMiHOG000233501.
    HOVERGENiHBG103071.
    InParanoidiP48303.
    KOiK04288.
    OMAiSAFIACW.
    OrthoDBiEOG708W0B.
    PhylomeDBiP48303.
    TreeFamiTF330052.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000987. EDG1_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR004061. S1P_rcpt.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00642. EDG1RECEPTOR.
    PR00237. GPCRRHODOPSN.
    PR01523. S1PRECEPTOR.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48303-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVSSTSIPVV KALRSQVSDY GNYDIIVRHY NYTGKLNIGV EKDHGIKLTS    50
    VVFILICCLI ILENIFVLLT IWKTKKFHRP MYYFIGNLAL SDLLAGVAYT 100
    ANLLLSGATT YKLTPAQWFL REGSMFVALS ASVFSLLAIA IERYITMLKM 150
    KLHNGSNSSR SFLLISACWV ISLILGGLPI MGWNCISSLS SCSTVLPLYH 200
    KHYILFCTTV FTLLLLSIVI LYCRIYSLVR TRSRRLTFRK NISKASRSSE 250
    KSLALLKTVI IVLSVFIACW APLFILLLLD VGCKAKTCDI LYKAEYFLVL 300
    AVLNSGTNPI IYTLTNKEMR RAFIRIISCC KCPNGDSAGK FKRPIIPGME 350
    FSRSKSDNSS HPQKDDGDNP ETIMSSGNVN SSS 383
    Length:383
    Mass (Da):42,746
    Last modified:February 1, 1996 - v1
    Checksum:i090BA6AEE09DB4F3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10303 mRNA. Translation: AAA83418.1.
    BC097938 mRNA. Translation: AAH97938.1.
    PIRiI53870.
    RefSeqiNP_058997.1. NM_017301.2.
    UniGeneiRn.109455.

    Genome annotation databases

    EnsembliENSRNOT00000018318; ENSRNOP00000052627; ENSRNOG00000013683.
    GeneIDi29733.
    KEGGirno:29733.
    UCSCiRGD:61958. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10303 mRNA. Translation: AAA83418.1 .
    BC097938 mRNA. Translation: AAH97938.1 .
    PIRi I53870.
    RefSeqi NP_058997.1. NM_017301.2.
    UniGenei Rn.109455.

    3D structure databases

    ProteinModelPortali P48303.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000052627.

    Chemistry

    BindingDBi P48303.
    ChEMBLi CHEMBL1914263.
    GuidetoPHARMACOLOGYi 275.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P48303.

    Proteomic databases

    PRIDEi P48303.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000018318 ; ENSRNOP00000052627 ; ENSRNOG00000013683 .
    GeneIDi 29733.
    KEGGi rno:29733.
    UCSCi RGD:61958. rat.

    Organism-specific databases

    CTDi 1901.
    RGDi 61958. S1pr1.

    Phylogenomic databases

    eggNOGi NOG148018.
    GeneTreei ENSGT00750000117544.
    HOGENOMi HOG000233501.
    HOVERGENi HBG103071.
    InParanoidi P48303.
    KOi K04288.
    OMAi SAFIACW.
    OrthoDBi EOG708W0B.
    PhylomeDBi P48303.
    TreeFami TF330052.

    Miscellaneous databases

    NextBioi 610218.
    PROi P48303.

    Gene expression databases

    Genevestigatori P48303.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000987. EDG1_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR004061. S1P_rcpt.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00642. EDG1RECEPTOR.
    PR00237. GPCRRHODOPSN.
    PR01523. S1PRECEPTOR.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the rat edg-1 immediate-early gene: expression pattern suggests diverse functions."
      Lado D.C., Browe C.S., Gaskin A.A., Borden J.M., Maclennan A.J.
      Gene 149:331-336(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
      Tissue: Cerebellum.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta.

    Entry informationi

    Entry nameiS1PR1_RAT
    AccessioniPrimary (citable) accession number: P48303
    Secondary accession number(s): Q4V7F6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3