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P48303 (S1PR1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sphingosine 1-phosphate receptor 1

Short name=S1P receptor 1
Short name=S1P1
Alternative name(s):
Endothelial differentiation G-protein coupled receptor 1
Sphingosine 1-phosphate receptor Edg-1
Short name=S1P receptor Edg-1
CD_antigen=CD363
Gene names
Name:S1pr1
Synonyms:Edg1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length383 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate. Required for normal embryonic heart development and normal cardiac morphogenesis. Plays an important role in the regulation of sprouting angiogenesis and vascular maturation. Inhibits sprouting angiogenesis to prevent excessive sprouting during blood vessel development. Required for normal egress of mature T-cells from the thymus into the blood stream and into peripheral lymphoid organs. Plays a role in the migration of osteoclast precursor cells, the regulation of bone mineralization and bone homeostasis. Plays a role in responses to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine by pulmonary endothelial cells and in the protection against ventilator-induced lung injury By similarity.

Subunit structure

Interacts with GNAI1 and GNAI3 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Endosome By similarity. Membrane raft By similarity. Note: Recruited to caveolin-enriched plasma membrane microdomains in response to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine. Ligand binding leads to receptor internalization By similarity.

Developmental stage

First detected at embryonic day 15. At postnatal day 14 detected in skin, spleen, liver, kidney, heart, testicle, lung and brain. At adulthood is most abundant in brain. Ref.1

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Biological processAngiogenesis
Chemotaxis
   Cellular componentCell membrane
Endosome
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMAcetylation
Disulfide bond
Glycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from mutant phenotype PubMed 11557736. Source: RGD

T cell migration

Inferred from sequence or structural similarity. Source: UniProtKB

actin cytoskeleton reorganization

Inferred from sequence or structural similarity. Source: UniProtKB

adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway

Inferred from mutant phenotype PubMed 9765227. Source: RGD

angiogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

blood vessel maturation

Inferred from sequence or structural similarity. Source: UniProtKB

brain development

Inferred from electronic annotation. Source: Ensembl

cardiac muscle tissue growth involved in heart morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

cell migration

Inferred from sequence or structural similarity. Source: UniProtKB

chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

endothelial cell differentiation

Inferred from expression pattern Ref.1. Source: RGD

heart trabecula morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

lamellipodium assembly

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of stress fiber assembly

Inferred from direct assay PubMed 11094076. Source: RGD

neuron differentiation

Inferred from expression pattern Ref.1. Source: RGD

positive regulation of Ras GTPase activity

Inferred from direct assay PubMed 14711826. Source: RGD

positive regulation of cell migration

Inferred from direct assay PubMed 11557736. Source: RGD

positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway

Inferred from mutant phenotype PubMed 9765227. Source: RGD

positive regulation of positive chemotaxis

Inferred from direct assay PubMed 11094076. Source: RGD

positive regulation of smooth muscle cell proliferation

Inferred from direct assay PubMed 11557736. Source: RGD

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 14711826. Source: RGD

regulation of bone mineralization

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of bone resorption

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of cell adhesion

Inferred from electronic annotation. Source: Ensembl

sphingosine-1-phosphate signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

transmission of nerve impulse

Inferred from expression pattern Ref.1. Source: RGD

   Cellular_componentendosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

external side of plasma membrane

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

intrinsic component of plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

membrane raft

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionG-protein coupled receptor activity

Inferred from mutant phenotype PubMed 11557736. Source: RGD

sphingolipid binding

Inferred from direct assay PubMed 9765227. Source: RGD

sphingosine-1-phosphate receptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 383383Sphingosine 1-phosphate receptor 1
PRO_0000069414

Regions

Topological domain1 – 4747Extracellular By similarity
Transmembrane48 – 6922Helical; Name=1; By similarity
Topological domain70 – 8314Cytoplasmic By similarity
Transmembrane84 – 10522Helical; Name=2; By similarity
Topological domain106 – 11712Extracellular By similarity
Transmembrane118 – 13922Helical; Name=3; By similarity
Topological domain140 – 16122Cytoplasmic By similarity
Transmembrane162 – 18322Helical; Name=4; By similarity
Topological domain184 – 19714Extracellular By similarity
Transmembrane198 – 22528Helical; Name=5; By similarity
Topological domain226 – 25833Cytoplasmic By similarity
Transmembrane259 – 27921Helical; Name=6; By similarity
Topological domain280 – 29011Extracellular By similarity
Transmembrane291 – 31121Helical; Name=7; By similarity
Topological domain312 – 38372Cytoplasmic By similarity
Region121 – 1222Sphingosine 1-phosphate binding By similarity
Region266 – 2705Sphingosine 1-phosphate binding By similarity

Amino acid modifications

Modified residue111N6-acetyllysine By similarity
Modified residue2371Phosphothreonine By similarity
Modified residue3541Phosphoserine Potential
Lipidation3291S-palmitoyl cysteine By similarity
Glycosylation311N-linked (GlcNAc...) Potential
Disulfide bond185 ↔ 192 By similarity
Disulfide bond283 ↔ 288 By similarity

Sequences

Sequence LengthMass (Da)Tools
P48303 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 090BA6AEE09DB4F3

FASTA38342,746
        10         20         30         40         50         60 
MVSSTSIPVV KALRSQVSDY GNYDIIVRHY NYTGKLNIGV EKDHGIKLTS VVFILICCLI 

        70         80         90        100        110        120 
ILENIFVLLT IWKTKKFHRP MYYFIGNLAL SDLLAGVAYT ANLLLSGATT YKLTPAQWFL 

       130        140        150        160        170        180 
REGSMFVALS ASVFSLLAIA IERYITMLKM KLHNGSNSSR SFLLISACWV ISLILGGLPI 

       190        200        210        220        230        240 
MGWNCISSLS SCSTVLPLYH KHYILFCTTV FTLLLLSIVI LYCRIYSLVR TRSRRLTFRK 

       250        260        270        280        290        300 
NISKASRSSE KSLALLKTVI IVLSVFIACW APLFILLLLD VGCKAKTCDI LYKAEYFLVL 

       310        320        330        340        350        360 
AVLNSGTNPI IYTLTNKEMR RAFIRIISCC KCPNGDSAGK FKRPIIPGME FSRSKSDNSS 

       370        380 
HPQKDDGDNP ETIMSSGNVN SSS 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of the rat edg-1 immediate-early gene: expression pattern suggests diverse functions."
Lado D.C., Browe C.S., Gaskin A.A., Borden J.M., Maclennan A.J.
Gene 149:331-336(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
Tissue: Cerebellum.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U10303 mRNA. Translation: AAA83418.1.
BC097938 mRNA. Translation: AAH97938.1.
PIRI53870.
RefSeqNP_058997.1. NM_017301.2.
UniGeneRn.109455.

3D structure databases

ProteinModelPortalP48303.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000052627.

Chemistry

BindingDBP48303.
ChEMBLCHEMBL1914263.
GuidetoPHARMACOLOGY275.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP48303.

Proteomic databases

PRIDEP48303.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000018318; ENSRNOP00000052627; ENSRNOG00000013683.
GeneID29733.
KEGGrno:29733.
UCSCRGD:61958. rat.

Organism-specific databases

CTD1901.
RGD61958. S1pr1.

Phylogenomic databases

eggNOGNOG148018.
GeneTreeENSGT00750000117544.
HOGENOMHOG000233501.
HOVERGENHBG103071.
InParanoidP48303.
KOK04288.
OMASAFIACW.
OrthoDBEOG708W0B.
PhylomeDBP48303.
TreeFamTF330052.

Gene expression databases

GenevestigatorP48303.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000987. EDG1_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR004061. S1P_rcpt.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00642. EDG1RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01523. S1PRECEPTOR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio610218.
PROP48303.

Entry information

Entry nameS1PR1_RAT
AccessionPrimary (citable) accession number: P48303
Secondary accession number(s): Q4V7F6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries