P48303 (S1PR1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sphingosine 1-phosphate receptor 1
Short name=S1P receptor 1
Short name=S1P1

Alternative name(s):
Endothelial differentiation G-protein coupled receptor 1
Sphingosine 1-phosphate receptor Edg-1
Short name=S1P receptor Edg-1
CD_antigen=CD363

Gene names

Name:	S1pr1
Synonyms:	Edg1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Developmental stage	First detected at embryonic day 15. At postnatal day 14 detected in skin, spleen, liver, kidney, heart, testicle, lung and brain. At adulthood is most abundant in brain.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Inferred from mutant phenotype PubMed 9765227. Source: RGD angiogenesis Inferred from electronic annotation. Source: Compara brain development Inferred from electronic annotation. Source: Compara elevation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Inferred from mutant phenotype PubMed 9765227. Source: RGD endothelial cell differentiation Inferred from expression pattern Ref.1. Source: RGD negative regulation of stress fiber assembly Inferred from direct assay PubMed 11094076. Source: RGD neuron differentiation Inferred from expression pattern Ref.1. Source: RGD positive regulation of Ras GTPase activity Inferred from direct assay PubMed 14711826. Source: RGD positive regulation of cell migration Inferred from direct assay PubMed 11557736. Source: RGD positive regulation of positive chemotaxis Inferred from direct assay PubMed 11094076. Source: RGD positive regulation of smooth muscle cell proliferation Inferred from direct assay PubMed 11557736. Source: RGD positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 14711826. Source: RGD regulation of cell adhesion Inferred from electronic annotation. Source: Compara sphingosine-1-phosphate signaling pathway Inferred from electronic annotation. Source: GOC transmission of nerve impulse Inferred from expression pattern Ref.1. Source: RGD
Cellular_component	external side of plasma membrane Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	G-protein coupled receptor activity Inferred from mutant phenotype PubMed 11557736. Source: RGD sphingolipid binding Inferred from direct assay PubMed 9765227. Source: RGD sphingosine-1-phosphate receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	Sphingosine 1-phosphate receptor 1		PRO_0000069414
Regions
	Topological domain	1 – 47	47	Extracellular By similarity
	Transmembrane	48 – 72	25	Helical; Name=1; By similarity
	Topological domain	73 – 79	7	Cytoplasmic By similarity
	Transmembrane	80 – 108	29	Helical; Name=2; By similarity
	Topological domain	109 – 122	14	Extracellular By similarity
	Transmembrane	123 – 141	19	Helical; Name=3; By similarity
	Topological domain	142 – 160	19	Cytoplasmic By similarity
	Transmembrane	161 – 186	26	Helical; Name=4; By similarity
	Topological domain	187 – 202	16	Extracellular By similarity
	Transmembrane	203 – 223	21	Helical; Name=5; By similarity
	Topological domain	224 – 257	34	Cytoplasmic By similarity
	Transmembrane	258 – 279	22	Helical; Name=6; By similarity
	Topological domain	280 – 295	16	Extracellular By similarity
	Transmembrane	296 – 316	21	Helical; Name=7; By similarity
	Topological domain	317 – 383	67	Cytoplasmic By similarity
Amino acid modifications
	Modified residue	237	1	Phosphothreonine By similarity
	Modified residue	354	1	Phosphoserine Potential
	Lipidation	329	1	S-palmitoyl cysteine By similarity
	Glycosylation	31	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P48303 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 090BA6AEE09DB4F3	FASTA	383	42,746
	10 20 30 40 50 60 MVSSTSIPVV KALRSQVSDY GNYDIIVRHY NYTGKLNIGV EKDHGIKLTS VVFILICCLI 70 80 90 100 110 120 ILENIFVLLT IWKTKKFHRP MYYFIGNLAL SDLLAGVAYT ANLLLSGATT YKLTPAQWFL 130 140 150 160 170 180 REGSMFVALS ASVFSLLAIA IERYITMLKM KLHNGSNSSR SFLLISACWV ISLILGGLPI 190 200 210 220 230 240 MGWNCISSLS SCSTVLPLYH KHYILFCTTV FTLLLLSIVI LYCRIYSLVR TRSRRLTFRK 250 260 270 280 290 300 NISKASRSSE KSLALLKTVI IVLSVFIACW APLFILLLLD VGCKAKTCDI LYKAEYFLVL 310 320 330 340 350 360 AVLNSGTNPI IYTLTNKEMR RAFIRIISCC KCPNGDSAGK FKRPIIPGME FSRSKSDNSS 370 380 HPQKDDGDNP ETIMSSGNVN SSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the rat edg-1 immediate-early gene: expression pattern suggests diverse functions." Lado D.C., Browe C.S., Gaskin A.A., Borden J.M., Maclennan A.J. Gene 149:331-336(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cerebellum.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10303 mRNA. Translation: AAA83418.1. BC097938 mRNA. Translation: AAH97938.1.
IPI	IPI00189818.
PIR	I53870.
RefSeq	NP_058997.1. NM_017301.2.
UniGene	Rn.109455.
3D structure databases
ProteinModelPortal	P48303.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000052627.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P48303.
Proteomic databases
PRIDE	P48303.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000018318; ENSRNOP00000052627; ENSRNOG00000013683.
GeneID	29733.
KEGG	rno:29733.
UCSC	RGD:61958. rat.
Organism-specific databases
CTD	1901.
RGD	61958. S1pr1.
Phylogenomic databases
eggNOG	NOG148018.
GeneTree	ENSGT00700000104088.
HOGENOM	HOG000233501.
HOVERGEN	HBG103071.
InParanoid	P48303.
KO	K04288.
OMA	SAFIACW.
OrthoDB	EOG418BNR.
Gene expression databases
Genevestigator	P48303.
GermOnline	ENSRNOG00000013683. Rattus norvegicus.
Family and domain databases
InterPro	IPR000987. EDG1_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR004061. S1P_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00642. EDG1RECEPTOR. PR00237. GPCRRHODOPSN. PR01523. S1PRECEPTOR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P48303.
ChEMBL	CHEMBL1914263.
NextBio	610218.

Entry information

Entry name

S1PR1_RAT

Accession

Primary (citable) accession number: P48303
Secondary accession number(s): Q4V7F6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program