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Protein

Sphingosine 1-phosphate receptor 1

Gene

S1pr1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate. Required for normal embryonic heart development and normal cardiac morphogenesis. Plays an important role in the regulation of sprouting angiogenesis and vascular maturation. Inhibits sprouting angiogenesis to prevent excessive sprouting during blood vessel development. Required for normal egress of mature T-cells from the thymus into the blood stream and into peripheral lymphoid organs. Plays a role in the migration of osteoclast precursor cells, the regulation of bone mineralization and bone homeostasis. Plays a role in responses to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine by pulmonary endothelial cells and in the protection against ventilator-induced lung injury (By similarity).By similarity

GO - Molecular functioni

  • G-protein coupled receptor activity Source: RGD
  • sphingolipid binding Source: RGD
  • sphingosine-1-phosphate receptor activity Source: UniProtKB

GO - Biological processi

  • actin cytoskeleton reorganization Source: UniProtKB
  • adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: RGD
  • angiogenesis Source: UniProtKB-KW
  • blood vessel maturation Source: UniProtKB
  • brain development Source: Ensembl
  • cardiac muscle tissue growth involved in heart morphogenesis Source: UniProtKB
  • cell migration Source: UniProtKB
  • chemotaxis Source: UniProtKB
  • endothelial cell differentiation Source: RGD
  • G-protein coupled receptor signaling pathway Source: RGD
  • heart trabecula morphogenesis Source: UniProtKB
  • lamellipodium assembly Source: UniProtKB
  • negative regulation of stress fiber assembly Source: RGD
  • neuron differentiation Source: RGD
  • positive regulation of cell migration Source: RGD
  • positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: RGD
  • positive regulation of GTPase activity Source: RGD
  • positive regulation of positive chemotaxis Source: RGD
  • positive regulation of smooth muscle cell proliferation Source: RGD
  • positive regulation of transcription from RNA polymerase II promoter Source: RGD
  • regulation of bone mineralization Source: UniProtKB
  • regulation of bone resorption Source: UniProtKB
  • regulation of cell adhesion Source: Ensembl
  • sphingosine-1-phosphate signaling pathway Source: UniProtKB
  • T cell migration Source: UniProtKB
  • transmission of nerve impulse Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Angiogenesis, Chemotaxis

Enzyme and pathway databases

ReactomeiREACT_303520. Lysosphingolipid and LPA receptors.
REACT_339234. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine 1-phosphate receptor 1
Short name:
S1P receptor 1
Short name:
S1P1
Alternative name(s):
Endothelial differentiation G-protein coupled receptor 1
Sphingosine 1-phosphate receptor Edg-1
Short name:
S1P receptor Edg-1
CD_antigen: CD363
Gene namesi
Name:S1pr1
Synonyms:Edg1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi61958. S1pr1.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity
  • Endosome By similarity
  • Membrane raft By similarity

  • Note: Recruited to caveolin-enriched plasma membrane microdomains in response to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine. Ligand binding leads to receptor internalization (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4747ExtracellularBy similarityAdd
BLAST
Transmembranei48 – 6922Helical; Name=1By similarityAdd
BLAST
Topological domaini70 – 8314CytoplasmicBy similarityAdd
BLAST
Transmembranei84 – 10522Helical; Name=2By similarityAdd
BLAST
Topological domaini106 – 11712ExtracellularBy similarityAdd
BLAST
Transmembranei118 – 13922Helical; Name=3By similarityAdd
BLAST
Topological domaini140 – 16122CytoplasmicBy similarityAdd
BLAST
Transmembranei162 – 18322Helical; Name=4By similarityAdd
BLAST
Topological domaini184 – 19714ExtracellularBy similarityAdd
BLAST
Transmembranei198 – 22528Helical; Name=5By similarityAdd
BLAST
Topological domaini226 – 25833CytoplasmicBy similarityAdd
BLAST
Transmembranei259 – 27921Helical; Name=6By similarityAdd
BLAST
Topological domaini280 – 29011ExtracellularBy similarityAdd
BLAST
Transmembranei291 – 31121Helical; Name=7By similarityAdd
BLAST
Topological domaini312 – 38372CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • cytosol Source: GOC
  • endosome Source: UniProtKB-SubCell
  • external side of plasma membrane Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • intrinsic component of plasma membrane Source: UniProtKB
  • membrane raft Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 383383Sphingosine 1-phosphate receptor 1PRO_0000069414Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111N6-acetyllysineBy similarity
Glycosylationi31 – 311N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi185 ↔ 192PROSITE-ProRule annotation
Modified residuei237 – 2371PhosphothreonineBy similarity
Disulfide bondi283 ↔ 288PROSITE-ProRule annotation
Lipidationi329 – 3291S-palmitoyl cysteineBy similarity
Modified residuei354 – 3541PhosphoserineSequence Analysis

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PRIDEiP48303.

PTM databases

PhosphoSiteiP48303.

Expressioni

Developmental stagei

First detected at embryonic day 15. At postnatal day 14 detected in skin, spleen, liver, kidney, heart, testicle, lung and brain. At adulthood is most abundant in brain.1 Publication

Gene expression databases

GenevisibleiP48303. RN.

Interactioni

Subunit structurei

Interacts with GNAI1 and GNAI3.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052627.

Structurei

3D structure databases

ProteinModelPortaliP48303.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni121 – 1222Sphingosine 1-phosphate bindingBy similarity
Regioni266 – 2705Sphingosine 1-phosphate bindingBy similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG148018.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000233501.
HOVERGENiHBG103071.
InParanoidiP48303.
KOiK04288.
OMAiSAFIACW.
OrthoDBiEOG708W0B.
PhylomeDBiP48303.
TreeFamiTF330052.

Family and domain databases

InterProiIPR000987. EDG1_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR004061. S1P_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00642. EDG1RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01523. S1PRECEPTOR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48303-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSSTSIPVV KALRSQVSDY GNYDIIVRHY NYTGKLNIGV EKDHGIKLTS
60 70 80 90 100
VVFILICCLI ILENIFVLLT IWKTKKFHRP MYYFIGNLAL SDLLAGVAYT
110 120 130 140 150
ANLLLSGATT YKLTPAQWFL REGSMFVALS ASVFSLLAIA IERYITMLKM
160 170 180 190 200
KLHNGSNSSR SFLLISACWV ISLILGGLPI MGWNCISSLS SCSTVLPLYH
210 220 230 240 250
KHYILFCTTV FTLLLLSIVI LYCRIYSLVR TRSRRLTFRK NISKASRSSE
260 270 280 290 300
KSLALLKTVI IVLSVFIACW APLFILLLLD VGCKAKTCDI LYKAEYFLVL
310 320 330 340 350
AVLNSGTNPI IYTLTNKEMR RAFIRIISCC KCPNGDSAGK FKRPIIPGME
360 370 380
FSRSKSDNSS HPQKDDGDNP ETIMSSGNVN SSS
Length:383
Mass (Da):42,746
Last modified:February 1, 1996 - v1
Checksum:i090BA6AEE09DB4F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10303 mRNA. Translation: AAA83418.1.
BC097938 mRNA. Translation: AAH97938.1.
PIRiI53870.
RefSeqiNP_058997.1. NM_017301.2.
XP_008759681.1. XM_008761459.1.
UniGeneiRn.109455.

Genome annotation databases

EnsembliENSRNOT00000018318; ENSRNOP00000052627; ENSRNOG00000013683.
GeneIDi29733.
KEGGirno:29733.
UCSCiRGD:61958. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10303 mRNA. Translation: AAA83418.1.
BC097938 mRNA. Translation: AAH97938.1.
PIRiI53870.
RefSeqiNP_058997.1. NM_017301.2.
XP_008759681.1. XM_008761459.1.
UniGeneiRn.109455.

3D structure databases

ProteinModelPortaliP48303.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052627.

Chemistry

BindingDBiP48303.
ChEMBLiCHEMBL1914263.
GuidetoPHARMACOLOGYi275.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP48303.

Proteomic databases

PRIDEiP48303.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018318; ENSRNOP00000052627; ENSRNOG00000013683.
GeneIDi29733.
KEGGirno:29733.
UCSCiRGD:61958. rat.

Organism-specific databases

CTDi1901.
RGDi61958. S1pr1.

Phylogenomic databases

eggNOGiNOG148018.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000233501.
HOVERGENiHBG103071.
InParanoidiP48303.
KOiK04288.
OMAiSAFIACW.
OrthoDBiEOG708W0B.
PhylomeDBiP48303.
TreeFamiTF330052.

Enzyme and pathway databases

ReactomeiREACT_303520. Lysosphingolipid and LPA receptors.
REACT_339234. G alpha (i) signalling events.

Miscellaneous databases

NextBioi610218.
PROiP48303.

Gene expression databases

GenevisibleiP48303. RN.

Family and domain databases

InterProiIPR000987. EDG1_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR004061. S1P_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00642. EDG1RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01523. S1PRECEPTOR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the rat edg-1 immediate-early gene: expression pattern suggests diverse functions."
    Lado D.C., Browe C.S., Gaskin A.A., Borden J.M., Maclennan A.J.
    Gene 149:331-336(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Tissue: Cerebellum.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.

Entry informationi

Entry nameiS1PR1_RAT
AccessioniPrimary (citable) accession number: P48303
Secondary accession number(s): Q4V7F6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 24, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.