P48301 (TEAD2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional enhancer factor TEF-4

Alternative name(s):
ETEF-1
Embryonic TEA domain-containing factor
Short name=ETF
TEA domain family member 2
Short name=TEAD-2

Gene names

Name:	Tead2
Synonyms:	Etdf, Etf, Tef4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction By similarity. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.
Subunit structure	Interacts with YAP1 and WWTR1/TAZ By similarity.
Subcellular location	Nucleus.
Tissue specificity	Highest expression in brain. High levels also found in lung, testis and ovarian follicle cells. Lower levels in heart and spleen.
Developmental stage	In the embryo, levels fall after fertilization until the 2-4 cell stage and then increase rapidly.
Sequence similarities	Contains 1 TEA DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of cell death Inferred from genetic interaction. Source: UniProtKB neural tube closure Inferred from mutant phenotype. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI
Cellular component	transcription factor complex Inferred from direct assay. Source: MGI
Molecular function	DNA binding Inferred from direct assay Ref.1. Source: MGI protein binding Inferred from physical interaction. Source: MGI sequence-specific distal enhancer binding RNA polymerase II transcription factor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Transcriptional enhancer factor TEF-4		PRO_0000205933
Regions
	DNA binding	40 – 107	68	TEA
	Region	172 – 445	274	Transcriptional activation Potential
	Compositional bias	152 – 216	65	Pro-rich
Experimental info
	Sequence conflict	289	1	Y → F in CAA64216. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P48301 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 237BC27A8BF9877C	FASTA	445	49,041
	10 20 30 40 50 60 MGDPRTGAPL DDGGGWTGSE EGSEEGTGGS EGVGGDGSPD AEGVWSPDIE QSFQEALAIY 70 80 90 100 110 120 PPCGRRKIIL SDEGKMYGRN ELIARYIKLR TGKTRTRKQV SSHIQVLARR KSREIQSKLK 130 140 150 160 170 180 DQVSKDKAFQ TMATMSSAQL ISAPSLQAKL GPSGPQATEL FQFWSGSSGP PWNVPDVKPF 190 200 210 220 230 240 SQAPFSVSLT PPASDLPGYE PPPALSPLPP PAPSPPAWQA RALGTARLQL IEFSAFVEPP 250 260 270 280 290 300 DAVDSFQRHL FVHISQQCPS PGAPPLESVD VRQIYDKFPE KKGGLRELYD RGPPHAFFLV 310 320 330 340 350 360 KFWADLNWGP SAEEAGSSGG GGGFYGVSSQ YESRELMTLT CSSKVCSFGK QVVEKVETER 370 380 390 400 410 420 AQLEDGRFVY RLLRSPMCEY LVNFLHKLRQ LPERYMMNSV LENFTILQVV TNRDTQELLL 430 440 CTAYVFEVST SERGAQYHIY RLVRD

References

[1]	"Molecular characterization of cDNA encoding a novel protein related to transcriptional enhancer factor-1 from neural precursor cells." Yasunami M., Suzuki K., Houtani T., Sugimoto T., Ohkubo H. J. Biol. Chem. 270:18649-18654(1995) [PubMed: 7629195] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICR. Tissue: Head.
[2]	"Structural organization and chromosomal assignment of the mouse embryonic TEA domain-containing factor (ETF) gene." Suzuki K., Yasunami M., Matsuda Y., Maeda T., Kobayashi H., Terasaki H., Ohkubo H. Genomics 36:263-270(1996) [PubMed: 8812452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6.
[3]	"A novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain." Jacquemin P., Hwang J.-J., Martial J.A., Dolle P., Davidson I. J. Biol. Chem. 271:21775-21785(1996) [PubMed: 8702974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Transcription factor mTEAD-2 is selectively expressed at the beginning of zygotic gene expression in the mouse." Kaneko K.J., Cullinan E.B., Latham K.E., Depamphilis M.L. Development 124:1963-1973(1997) [PubMed: 9169843] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50563 mRNA. Translation: BAA09126.1. D83596 Genomic DNA. Translation: BAA12018.1. X94442 mRNA. Translation: CAA64216.1. Y10026 mRNA. Translation: CAA71135.1.
IPI	IPI00110847.
PIR	A57400.
RefSeq	NP_035695.1. NM_011565.2.
UniGene	Mm.3019.
3D structure databases
ProteinModelPortal	P48301.
SMR	P48301. Positions 40-115, 221-444.
ModBase	Search...
Protein-protein interaction databases
STRING	P48301.
PTM databases
PhosphoSite	P48301.
Proteomic databases
PRIDE	P48301.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000033060; ENSMUSP00000033060; ENSMUSG00000030796. ENSMUST00000107801; ENSMUSP00000103431; ENSMUSG00000030796.
GeneID	21677.
KEGG	mmu:21677.
Organism-specific databases
CTD	8463.
MGI	MGI:104904. Tead2.
Phylogenomic databases
eggNOG	roNOG11312.
HOGENOM	HBG402953.
HOVERGEN	HBG056905.
InParanoid	P48301.
OMA	MTLTCSS.
OrthoDB	EOG483D4Q.
PhylomeDB	P48301.
Gene expression databases
ArrayExpress	P48301.
Bgee	P48301.
CleanEx	MM_TEAD2.
Genevestigator	P48301.
GermOnline	ENSMUSG00000030796. Mus musculus.
Family and domain databases
InterPro	IPR000818. TEA/ATTS. IPR016361. TEF. [Graphical view]
KO	K09449.
PANTHER	PTHR11834. TEA/ATTS. 1 hit.
Pfam	PF01285. TEA. 1 hit. [Graphical view]
PIRSF	PIRSF002603. TEF. 1 hit.
PRINTS	PR00065. TEADOMAIN.
SMART	SM00426. TEA. 1 hit. [Graphical view]
PROSITE	PS00554. TEA_1. 1 hit. PS51088. TEA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	300972.
SOURCE	Search...

Entry information

Entry name

TEAD2_MOUSE

Accession

Primary (citable) accession number: P48301
Secondary accession number(s): Q62297

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 16, 2011
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program