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P48301 (TEAD2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional enhancer factor TEF-4
Alternative name(s):
ETEF-1
Embryonic TEA domain-containing factor
Short name=ETF
TEA domain family member 2
Short name=TEAD-2
Gene names
Name:Tead2
Synonyms:Etdf, Etf, Tef4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction By similarity. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.

Subunit structure

Interacts with YAP1 and WWTR1/TAZ By similarity.

Subcellular location

Nucleus.

Tissue specificity

Highest expression in brain. High levels also found in lung, testis and ovarian follicle cells. Lower levels in heart and spleen.

Developmental stage

In the embryo, levels fall after fertilization until the 2-4 cell stage and then increase rapidly.

Sequence similarities

Contains 1 TEA DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionActivator
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processembryonic heart tube morphogenesis

Inferred from genetic interaction PubMed 18332127. Source: MGI

hippo signaling

Inferred from electronic annotation. Source: InterPro

lateral mesoderm development

Inferred from genetic interaction PubMed 18332127. Source: MGI

negative regulation of cell death

Inferred from genetic interaction PubMed 20596238. Source: UniProtKB

neural tube closure

Inferred from mutant phenotype PubMed 17868131. Source: MGI

notochord development

Inferred from genetic interaction PubMed 18332127. Source: MGI

paraxial mesoderm development

Inferred from genetic interaction PubMed 18332127. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 14736747PubMed 9502435. Source: MGI

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 14736747. Source: GOC

vasculogenesis

Inferred from genetic interaction PubMed 18332127. Source: MGI

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

transcription factor complex

Inferred from direct assay PubMed 16207754. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 16207754Ref.1PubMed 9502435. Source: MGI

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 14736747. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445Transcriptional enhancer factor TEF-4
PRO_0000205933

Regions

DNA binding40 – 10768TEA
Region172 – 445274Transcriptional activation Potential
Compositional bias152 – 21665Pro-rich

Experimental info

Sequence conflict2891Y → F in CAA64216. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P48301 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 237BC27A8BF9877C

FASTA44549,041
        10         20         30         40         50         60 
MGDPRTGAPL DDGGGWTGSE EGSEEGTGGS EGVGGDGSPD AEGVWSPDIE QSFQEALAIY 

        70         80         90        100        110        120 
PPCGRRKIIL SDEGKMYGRN ELIARYIKLR TGKTRTRKQV SSHIQVLARR KSREIQSKLK 

       130        140        150        160        170        180 
DQVSKDKAFQ TMATMSSAQL ISAPSLQAKL GPSGPQATEL FQFWSGSSGP PWNVPDVKPF 

       190        200        210        220        230        240 
SQAPFSVSLT PPASDLPGYE PPPALSPLPP PAPSPPAWQA RALGTARLQL IEFSAFVEPP 

       250        260        270        280        290        300 
DAVDSFQRHL FVHISQQCPS PGAPPLESVD VRQIYDKFPE KKGGLRELYD RGPPHAFFLV 

       310        320        330        340        350        360 
KFWADLNWGP SAEEAGSSGG GGGFYGVSSQ YESRELMTLT CSSKVCSFGK QVVEKVETER 

       370        380        390        400        410        420 
AQLEDGRFVY RLLRSPMCEY LVNFLHKLRQ LPERYMMNSV LENFTILQVV TNRDTQELLL 

       430        440 
CTAYVFEVST SERGAQYHIY RLVRD 

« Hide

References

[1]"Molecular characterization of cDNA encoding a novel protein related to transcriptional enhancer factor-1 from neural precursor cells."
Yasunami M., Suzuki K., Houtani T., Sugimoto T., Ohkubo H.
J. Biol. Chem. 270:18649-18654(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ICR.
Tissue: Head.
[2]"Structural organization and chromosomal assignment of the mouse embryonic TEA domain-containing factor (ETF) gene."
Suzuki K., Yasunami M., Matsuda Y., Maeda T., Kobayashi H., Terasaki H., Ohkubo H.
Genomics 36:263-270(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C57BL/6.
[3]"A novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain."
Jacquemin P., Hwang J.-J., Martial J.A., Dolle P., Davidson I.
J. Biol. Chem. 271:21775-21785(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Transcription factor mTEAD-2 is selectively expressed at the beginning of zygotic gene expression in the mouse."
Kaneko K.J., Cullinan E.B., Latham K.E., Depamphilis M.L.
Development 124:1963-1973(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D50563 mRNA. Translation: BAA09126.1.
D83596 Genomic DNA. Translation: BAA12018.1.
X94442 mRNA. Translation: CAA64216.1.
Y10026 mRNA. Translation: CAA71135.1.
PIRA57400.
RefSeqNP_001272427.1. NM_001285498.1.
NP_035695.1. NM_011565.2.
XP_006540840.1. XM_006540777.1.
UniGeneMm.3019.

3D structure databases

ProteinModelPortalP48301.
SMRP48301. Positions 40-115, 221-444.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204099. 3 interactions.

PTM databases

PhosphoSiteP48301.

Proteomic databases

PRIDEP48301.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033060; ENSMUSP00000033060; ENSMUSG00000030796.
ENSMUST00000107801; ENSMUSP00000103431; ENSMUSG00000030796.
GeneID21677.
KEGGmmu:21677.
UCSCuc009gud.1. mouse.

Organism-specific databases

CTD8463.
MGIMGI:104904. Tead2.

Phylogenomic databases

eggNOGNOG246992.
HOGENOMHOG000253933.
HOVERGENHBG056905.
InParanoidP48301.
KOK09448.
OMAGSGTPWN.
OrthoDBEOG7288RP.
PhylomeDBP48301.
TreeFamTF313443.

Gene expression databases

BgeeP48301.
CleanExMM_TEAD2.
GenevestigatorP48301.

Family and domain databases

InterProIPR000818. TEA/ATTS.
IPR016361. TEF.
[Graphical view]
PANTHERPTHR11834. PTHR11834. 1 hit.
PfamPF01285. TEA. 1 hit.
[Graphical view]
PIRSFPIRSF002603. TEF. 1 hit.
PRINTSPR00065. TEADOMAIN.
SMARTSM00426. TEA. 1 hit.
[Graphical view]
PROSITEPS00554. TEA_1. 1 hit.
PS51088. TEA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTEAD2. mouse.
NextBio300972.
PROP48301.
SOURCESearch...

Entry information

Entry nameTEAD2_MOUSE
AccessionPrimary (citable) accession number: P48301
Secondary accession number(s): Q62297
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot