P48300 (FUCO_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tissue alpha-L-fucosidase
EC=3.2.1.51

Alternative name(s):
Alpha-L-fucosidase I
Alpha-L-fucoside fucohydrolase 1
Short name=Alpha-L-fucosidase 1

Gene names

Name:

FUCA1

Organism

Canis familiaris (Dog) (Canis lupus familiaris)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.
Catalytic activity	An alpha-L-fucoside + H2O = L-fucose + an alcohol.
Subunit structure	Homotetramer By similarity.
Subcellular location	Lysosome.
Involvement in disease	Note=Defects in FUCA1 are the cause of fucosidosis. It is a lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Ref.1 Ref.2
Sequence similarities	Belongs to the glycosyl hydrolase 29 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fucose metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-L-fucosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 465	439	Tissue alpha-L-fucosidase		PRO_0000010307
Sites
	Site	296	1	May be important for catalysis
Amino acid modifications
	Glycosylation	241	1	N-linked (GlcNAc...) Potential
	Glycosylation	268	1	N-linked (GlcNAc...) Potential
	Glycosylation	382	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	14 – 15	2	AV → LPL in AAB17403. Ref.2
	Sequence conflict	14 – 15	2	AV → LPL in AAB17401. Ref.2
	Sequence conflict	30 – 32	3	AAA → GPP in AAB17403. Ref.2
	Sequence conflict	30 – 32	3	AAA → GPP in AAB17401. Ref.2
	Sequence conflict	66	1	E → V in AAB17403. Ref.2
	Sequence conflict	66	1	E → V in AAB17401. Ref.2
	Sequence conflict	234	1	K → E in AAB17403. Ref.2
	Sequence conflict	257	1	D → G in CAA63197. Ref.1
	Sequence conflict	291	1	L → H in AAB17403. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P48300 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 832BA3C3510341B7	FASTA	465	53,757
	10 20 30 40 50 60 MKPWAVGLGP PPPAVPLLLL LLLGAALVRA AAPPRRYTPD WQSLDSRPLP DWFDKAKFGV 70 80 90 100 110 120 FVHWGEFAVP AWGSEWFWWH WKGEGLPQYE QFMSENYPPG FSYADFGPQF TARFFHPDTW 130 140 150 160 170 180 ADLFQAAGAR YVVLTTKHHE GFTNWPSSVS WNWNSNDVGP HRDLVGELGR ALRKRNIRYG 190 200 210 220 230 240 LYHSLLEWFH PLYLLDKKNN FKTQFFVRAK TMPELYDLVN RYEPDLIWSD GEWKCPDTYW 250 260 270 280 290 300 NSTEFLSWLY NDSPVKDHVV VNDRWGQNCS CHHGGYYNCQ DKYKPESLPD LKWEMCTSID 310 320 330 340 350 360 KVSWGYRRNM VMSDVASECE IISELVQTVS LGGNYLLNIG PTKDGLIVPI FQERLLSIGK 370 380 390 400 410 420 WLSINGEAIY ASKPWRVQLE KNTTSVWYTS RGMTVYAIFL RWPENGVLSL KSPVTTSTTQ 430 440 450 460 ITMLGIQKDL KWSTEPEGLL IYLPQLSLFT LPVEFGWTIK LTGVE

References

[1]	"The molecular defect underlying canine fucosidosis." Skelly B.J., Sargan D.R., Herrtage M.E., Winchester B.G. J. Med. Genet. 33:284-288(1996) [PubMed: 8730282] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], DISEASE. Strain: English Springer spaniel. Tissue: Blood and Liver.
[2]	"Isolation of the canine alpha-L-fucosidase cDNA and definition of the fucosidosis mutation in English Springer Spaniels." Occhiodoro T., Anson D.S. Mamm. Genome 7:271-274(1996) [PubMed: 8661697] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], DISEASE. Strain: English Springer spaniel.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X92671 , X92672, X92673, X92674, X92675, X92676, X92677, X92678 Genomic DNA. Translation: CAA63362.1. X92448 mRNA. Translation: CAA63197.1. U29765 mRNA. Translation: AAB17403.1. U29766 Genomic DNA. Translation: AAB17401.1.
RefSeq	NP_001003250.1. NM_001003250.1.
UniGene	Cfa.3785.
3D structure databases
ProteinModelPortal	P48300.
ModBase	Search...
Protein-protein interaction databases
STRING	P48300.
Protein family/group databases
CAZy	GH29. Glycoside Hydrolase Family 29.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	403929.
KEGG	cfa:403929.
Organism-specific databases
CTD	2517.
Phylogenomic databases
eggNOG	maNOG11538.
GeneTree	ENSGT00440000035378.
HOVERGEN	HBG002155.
InParanoid	P48300.
OrthoDB	EOG4BRWKH.
Enzyme and pathway databases
BRENDA	3.2.1.51. 1154.
Family and domain databases
InterPro	IPR000933. Glyco_hydro_29. IPR018526. Glyco_hydro_29_CS. IPR016286. Glyco_hydro_29_sub. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K01206.
PANTHER	PTHR10030. Glyco_hydro_29. 1 hit.
Pfam	PF01120. Alpha_L_fucos. 1 hit. [Graphical view]
PIRSF	PIRSF001092. Alpha-L-fucosidase. 1 hit.
PRINTS	PR00741. GLHYDRLASE29.
SMART	SM00812. Alpha_L_fucos. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00385. ALPHA_L_FUCOSIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUCO_CANFA

Accession

Primary (citable) accession number: P48300

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 16, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program