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Protein

Carbonic anhydrase 4

Gene

Ca4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 (By similarity).By similarity

Catalytic activityi

H2CO3 = CO2 + H2O.

Cofactori

Zn2+By similarity

Enzyme regulationi

Inhibited by acetazolamide.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei87 – 871Proton acceptorBy similarity
Metal bindingi114 – 1141Zinc; catalyticBy similarity
Metal bindingi116 – 1161Zinc; catalyticBy similarity
Metal bindingi139 – 1391Zinc; catalyticBy similarity

GO - Molecular functioni

  • carbonate dehydratase activity Source: RGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • animal organ development Source: RGD
  • one-carbon metabolic process Source: InterPro
  • response to drug Source: RGD
  • response to steroid hormone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Carbonic anhydrase 4 (EC:4.2.1.1)
Alternative name(s):
Carbonate dehydratase IV
Carbonic anhydrase IV
Short name:
CA-IV
Gene namesi
Name:Ca4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2242. Ca4.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • apical plasma membrane Source: RGD
  • sarcolemma Source: RGD
  • sarcoplasmic reticulum Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence analysisAdd
BLAST
Chaini18 – 281264Carbonic anhydrase 4PRO_0000004232Add
BLAST
Propeptidei282 – 30928Removed in mature formBy similarityPRO_0000004233Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi23 ↔ 35By similarity
Disulfide bondi45 ↔ 226By similarity
Glycosylationi193 – 1931N-linked (GlcNAc...)Sequence analysis
Lipidationi281 – 2811GPI-anchor amidated serineBy similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP48284.
PRIDEiP48284.

PTM databases

iPTMnetiP48284.
PhosphoSiteiP48284.
UniCarbKBiP48284.

Expressioni

Tissue specificityi

Present in kidney and lung. Also particularly abundant in brain, muscle, heart and liver. Not detected in skin or spleen.1 Publication

Gene expression databases

BgeeiENSRNOG00000002916.
GenevisibleiP48284. RN.

Interactioni

Subunit structurei

Interacts with SLC4A4.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003908.

Structurei

3D structure databases

ProteinModelPortaliP48284.
SMRiP48284. Positions 22-283.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 282263Alpha-carbonic anhydrasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni222 – 2232Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the alpha-carbonic anhydrase family.Curated
Contains 1 alpha-carbonic anhydrase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0382. Eukaryota.
COG3338. LUCA.
HOGENOMiHOG000112637.
HOVERGENiHBG002837.
InParanoidiP48284.
KOiK18246.
OrthoDBiEOG091G0XFM.
PhylomeDBiP48284.
TreeFamiTF316425.

Family and domain databases

Gene3Di3.10.200.10. 1 hit.
InterProiIPR001148. Carbonic_anhydrase_a.
IPR023561. Carbonic_anhydrase_a-class.
IPR018338. Carbonic_anhydrase_a-class_CS.
IPR018343. Carbonic_anhydrase_CA4.
[Graphical view]
PANTHERiPTHR18952. PTHR18952. 1 hit.
PTHR18952:SF95. PTHR18952:SF95. 1 hit.
PfamiPF00194. Carb_anhydrase. 1 hit.
[Graphical view]
SMARTiSM01057. Carb_anhydrase. 1 hit.
[Graphical view]
SUPFAMiSSF51069. SSF51069. 1 hit.
PROSITEiPS00162. ALPHA_CA_1. 1 hit.
PS51144. ALPHA_CA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48284-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLLLALLAL AYVAPSTEDS HWCYEIQAKE PNSHCSGPEQ WTGDCKKNQQ
60 70 80 90 100
SPINIVTSKT KLNPSLTPFT FVGYDQKKKW EVKNNQHSVE MSLGEDIYIF
110 120 130 140 150
GGDLPTQYKA IQLHLHWSEE SNKGSEHSID GKHFAMEMHV VHKKMTTGDK
160 170 180 190 200
VQDSDSKDKI AVLAFMVEVG NEVNEGFQPL VEALSRLSKP FTNSTVSESC
210 220 230 240 250
LQDMLPEKKK LSAYFRYQGS LTTPGCDETV IWTVFEEPIK IHKDQFLEFS
260 270 280 290 300
KKLYYDQEQK LNMKDNVRPL QPLGNRQVFR SHASGRLLSL PLPTLLVPTL

TCLVASFLH
Length:309
Mass (Da):35,076
Last modified:February 1, 1996 - v1
Checksum:i9EBD1315348CFC8C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S68245 mRNA. Translation: AAB29505.1.
BC097329 mRNA. Translation: AAH97329.1.
PIRiI51900.
RefSeqiNP_062047.1. NM_019174.3.
UniGeneiRn.51389.

Genome annotation databases

GeneIDi29242.
KEGGirno:29242.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S68245 mRNA. Translation: AAB29505.1.
BC097329 mRNA. Translation: AAH97329.1.
PIRiI51900.
RefSeqiNP_062047.1. NM_019174.3.
UniGeneiRn.51389.

3D structure databases

ProteinModelPortaliP48284.
SMRiP48284. Positions 22-283.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003908.

PTM databases

iPTMnetiP48284.
PhosphoSiteiP48284.
UniCarbKBiP48284.

Proteomic databases

PaxDbiP48284.
PRIDEiP48284.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29242.
KEGGirno:29242.

Organism-specific databases

CTDi12351.
RGDi2242. Ca4.

Phylogenomic databases

eggNOGiKOG0382. Eukaryota.
COG3338. LUCA.
HOGENOMiHOG000112637.
HOVERGENiHBG002837.
InParanoidiP48284.
KOiK18246.
OrthoDBiEOG091G0XFM.
PhylomeDBiP48284.
TreeFamiTF316425.

Miscellaneous databases

PROiP48284.

Gene expression databases

BgeeiENSRNOG00000002916.
GenevisibleiP48284. RN.

Family and domain databases

Gene3Di3.10.200.10. 1 hit.
InterProiIPR001148. Carbonic_anhydrase_a.
IPR023561. Carbonic_anhydrase_a-class.
IPR018338. Carbonic_anhydrase_a-class_CS.
IPR018343. Carbonic_anhydrase_CA4.
[Graphical view]
PANTHERiPTHR18952. PTHR18952. 1 hit.
PTHR18952:SF95. PTHR18952:SF95. 1 hit.
PfamiPF00194. Carb_anhydrase. 1 hit.
[Graphical view]
SMARTiSM01057. Carb_anhydrase. 1 hit.
[Graphical view]
SUPFAMiSSF51069. SSF51069. 1 hit.
PROSITEiPS00162. ALPHA_CA_1. 1 hit.
PS51144. ALPHA_CA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAH4_RAT
AccessioniPrimary (citable) accession number: P48284
Secondary accession number(s): Q4QR97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 7, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.