P48284 (CAH4_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbonic anhydrase 4
EC=4.2.1.1

Alternative name(s):
Carbonate dehydratase IV
Carbonic anhydrase IV
Short name=CA-IV

Gene names

Name:

Ca4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 By similarity.
Catalytic activity	H2CO3 = CO2 + H2O.
Cofactor	Zinc By similarity.
Enzyme regulation	Inhibited by acetazolamide By similarity.
Subunit structure	Interacts with SLC4A4 By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor By similarity.
Tissue specificity	Present in kidney and lung. Also particularly abundant in brain, muscle, heart and liver. Not detected in skin or spleen. Ref.3
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the alpha-carbonic anhydrase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Lyase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: InterPro organ development Inferred from expression pattern. Source: RGD response to drug Inferred from expression pattern. Source: RGD response to steroid hormone stimulus Inferred from expression pattern. Source: RGD
Cellular component	apical plasma membrane Inferred from direct assay. Source: RGD sarcolemma Inferred from direct assay. Source: RGD sarcoplasmic reticulum Inferred from direct assay. Source: RGD
Molecular function	carbonate dehydratase activity Inferred from direct assay. Source: RGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 281	264	Carbonic anhydrase 4		PRO_0000004232
	Propeptide	282 – 309	28	Removed in mature form By similarity		PRO_0000004233
Regions
	Region	222 – 223	2	Substrate binding By similarity
Sites
	Active site	87	1	Proton acceptor By similarity
	Metal binding	114	1	Zinc; catalytic By similarity
	Metal binding	116	1	Zinc; catalytic By similarity
	Metal binding	139	1	Zinc; catalytic By similarity
Amino acid modifications
	Lipidation	281	1	GPI-anchor amidated serine By similarity
	Glycosylation	193	1	N-linked (GlcNAc...) Potential
	Disulfide bond	23 ↔ 35		By similarity
	Disulfide bond	45 ↔ 226		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48284 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 9EBD1315348CFC8C	FASTA	309	35,076
	10 20 30 40 50 60 MQLLLALLAL AYVAPSTEDS HWCYEIQAKE PNSHCSGPEQ WTGDCKKNQQ SPINIVTSKT 70 80 90 100 110 120 KLNPSLTPFT FVGYDQKKKW EVKNNQHSVE MSLGEDIYIF GGDLPTQYKA IQLHLHWSEE 130 140 150 160 170 180 SNKGSEHSID GKHFAMEMHV VHKKMTTGDK VQDSDSKDKI AVLAFMVEVG NEVNEGFQPL 190 200 210 220 230 240 VEALSRLSKP FTNSTVSESC LQDMLPEKKK LSAYFRYQGS LTTPGCDETV IWTVFEEPIK 250 260 270 280 290 300 IHKDQFLEFS KKLYYDQEQK LNMKDNVRPL QPLGNRQVFR SHASGRLLSL PLPTLLVPTL TCLVASFLH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pulmonary carbonic anhydrase IV: developmental regulation and cell-specific expression in the capillary endothelium." Fleming R.E., Crouch E.C., Ruzicka C.A., Sly W.S. Am. J. Physiol. 265:L627-L635(1993) [PubMed: 8279579] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Lung.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[3]	"Membrane-associated carbonic anhydrase from rat lung. Purification, characterization, tissue distribution, and comparison with carbonic anhydrase IVs of other mammals." Waheed A., Zhu X.L., Sly W.S. J. Biol. Chem. 267:3308-3311(1992) [PubMed: 1737787] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-22; 24-33 AND 36-38, CHARACTERIZATION, TISSUE SPECIFICITY. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S68245 mRNA. Translation: AAB29505.1. BC097329 mRNA. Translation: AAH97329.1.
IPI	IPI00189083.
PIR	I51900.
RefSeq	NP_062047.1. NM_019174.1.
UniGene	Rn.51389.
3D structure databases
ProteinModelPortal	P48284.
SMR	P48284. Positions 22-283.
ModBase	Search...
Protein-protein interaction databases
STRING	P48284.
Proteomic databases
PRIDE	P48284.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000003908; ENSRNOP00000003908; ENSRNOG00000002916.
GeneID	29242.
KEGG	rno:29242.
UCSC	NM_019174. rat.
Organism-specific databases
CTD	12351.
RGD	2242. Ca4.
Phylogenomic databases
eggNOG	roNOG16065.
GeneTree	ENSGT00570000078862.
HOVERGEN	HBG002837.
OrthoDB	EOG4FXR84.
PhylomeDB	P48284.
Gene expression databases
ArrayExpress	P48284.
Genevestigator	P48284.
GermOnline	ENSRNOG00000002916. Rattus norvegicus.
Family and domain databases
InterPro	IPR001148. a_carbonic_anhydrase. IPR023561. Carbonic_anhydrase_a-class. IPR018338. Carbonic_anhydrase_a-class_CS. IPR018343. Carbonic_anhydrase_CA4. [Graphical view]
Gene3D	G3DSA:3.10.200.10. Euk_COanhd. 1 hit.
KO	K01672.
PANTHER	PTHR18952:SF5. Carbonic_anhydrase_CA4. 1 hit. PTHR18952. Euk_COanhd. 1 hit.
Pfam	PF00194. Carb_anhydrase. 1 hit. [Graphical view]
SMART	SM01057. Carb_anhydrase. 1 hit. [Graphical view]
SUPFAM	SSF51069. Euk_COanhd. 1 hit.
PROSITE	PS00162. ALPHA_CA_1. 1 hit. PS51144. ALPHA_CA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	608518.

Entry information

Entry name

CAH4_RAT

Accession

Primary (citable) accession number: P48284
Secondary accession number(s): Q4QR97

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program