P48283 (CAH4_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbonic anhydrase 4
EC=4.2.1.1

Alternative name(s):
Carbonate dehydratase IV
Carbonic anhydrase IV
Short name=CA-IV

Gene names

Name:

CA4

Organism

Oryctolagus cuniculus (Rabbit) [Complete proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 By similarity.
Catalytic activity	H2CO3 = CO2 + H2O.
Cofactor	Zinc By similarity.
Enzyme regulation	Inhibited by acetazolamide By similarity.
Subunit structure	Interacts with SLC4A4. Ref.3
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor By similarity.
Sequence similarities	Belongs to the alpha-carbonic anhydrase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Lyase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	carbonate dehydratase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 280	262	Carbonic anhydrase 4		PRO_0000004230
	Propeptide	281 – 308	28	Removed in mature form By similarity		PRO_0000004231
Regions
	Region	221 – 222	2	Substrate binding By similarity
Sites
	Active site	86	1	Proton acceptor By similarity
	Metal binding	113	1	Zinc; catalytic By similarity
	Metal binding	115	1	Zinc; catalytic By similarity
	Metal binding	138	1	Zinc; catalytic By similarity
Amino acid modifications
	Lipidation	280	1	GPI-anchor amidated serine By similarity
	Glycosylation	31	1	N-linked (GlcNAc...) Potential
	Glycosylation	192	1	N-linked (GlcNAc...) Potential
	Disulfide bond	24 ↔ 34		By similarity
	Disulfide bond	44 ↔ 225		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48283 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: CDB29AED9D8CDEBC	FASTA	308	34,394
	10 20 30 40 50 60 MQLLFALLAL GALRPLAGEE LHWCYEIQAS NYSCLGPDKW QEDCQKSRQS PINIVTTKAE 70 80 90 100 110 120 VDHSLGRFHF SGYDQREARL VENNGHSVMV SLGDEISISG GGLPARYRAT QLHLHWSQEL 130 140 150 160 170 180 DRGSEHSLDG ERSAMEMHIV HQKETGTSGN EVQDSDDSIA VLAFLVEAGP TMNEGFQPLV 190 200 210 220 230 240 TALSAISIPG TNTTMAPSSL WDLLPAEEEL RHYFRYMGSL TTPACSETVV WTVFQEPIRL 250 260 270 280 290 300 HRDQILEFSS KLYYDQERKM NMKDNVRPLQ RLGDRSVFKS QAAGQLLPLP LPTLLVPTLA CVMAGLLR

References

[1]	"Expression of carbonic anhydrase IV mRNA in rabbit kidney: stimulation by metabolic acidosis." Winkler C.A., Kittelberger A.M., Schwartz G.J. Am. J. Physiol. 272:F551-F560(1997) [PubMed: 9140058] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: New Zealand white. Tissue: Kidney cortex.
[2]	Tamai S. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: New Zealand white. Tissue: Kidney cortex.
[3]	"Direct extracellular interaction between carbonic anhydrase IV and the human NBC1 sodium/bicarbonate co-transporter." Alvarez B.V., Loiselle F.B., Supuran C.T., Schwartz G.J., Casey J.R. Biochemistry 42:12321-12329(2003) [PubMed: 14567693] [Abstract] Cited for: INTERACTION WITH SLC4A4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L48928 mRNA. Translation: AAC37337.1. U58871 mRNA. Translation: AAB09467.1.
RefSeq	NP_001075841.1. NM_001082372.1.
UniGene	Ocu.2117.
3D structure databases
ProteinModelPortal	P48283.
SMR	P48283. Positions 23-280.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100009226.
Organism-specific databases
CTD	762.
Phylogenomic databases
eggNOG	maNOG17562.
GeneTree	ENSGT00570000078862.
HOVERGEN	HBG002837.
OrthoDB	EOG4FXR84.
Family and domain databases
InterPro	IPR001148. a_carbonic_anhydrase. IPR023561. Carbonic_anhydrase_a-class. IPR018343. Carbonic_anhydrase_CA4. [Graphical view]
Gene3D	G3DSA:3.10.200.10. Euk_COanhd. 1 hit.
PANTHER	PTHR18952:SF5. Carbonic_anhydrase_CA4. 1 hit. PTHR18952. Euk_COanhd. 1 hit.
Pfam	PF00194. Carb_anhydrase. 1 hit. [Graphical view]
SMART	SM01057. Carb_anhydrase. 1 hit. [Graphical view]
SUPFAM	SSF51069. Euk_COanhd. 1 hit.
PROSITE	PS00162. ALPHA_CA_1. False negative. PS51144. ALPHA_CA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAH4_RABIT

Accession

Primary (citable) accession number: P48283

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program