P48246 (HEM4_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uroporphyrinogen-III synthase
Short name=UROS
EC=4.2.1.75

Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase

Gene names

Name:	hemD
Ordered Locus Names:	PA5259

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III By similarity.
Catalytic activity	Hydroxymethylbilane = uroporphyrinogen III + H2O.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.
Sequence similarities	Belongs to the uroporphyrinogen-III synthase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Molecular function	Lyase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	uroporphyrinogen-III synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 251

251

Uroporphyrinogen-III synthase

PRO_0000135245

Experimental info

Sequence conflict

195 – 203

9

QNLYQLAAA → PKSVSVGGS in AAA18908. Ref.1

Secondary structure

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251

Helix Strand Turn

Details...

Beta strand

5 – 8

4

Helix

12 – 24

13

Beta strand

28 – 31

4

Beta strand

36 – 39

4

Helix

44 – 51

8

Helix

53 – 55

3

Beta strand

57 – 61

5

Helix

64 – 77

14

Beta strand

85 – 90

6

Helix

91 – 100

10

Helix

113 – 117

5

Helix

120 – 125

6

Beta strand

128 – 130

3

Beta strand

132 – 137

6

Helix

144 – 151

8

Beta strand

155 – 160

6

Beta strand

162 – 165

4

Helix

173 – 180

8

Beta strand

185 – 187

3

Helix

191 – 201

11

Helix

202 – 204

3

Helix

205 – 208

4

Beta strand

213 – 217

5

Helix

218 – 226

9

Beta strand

230 – 234

5

Beta strand

236 – 239

4

Helix

240 – 249

10

Sequences

	Sequence		Length	Mass (Da)
	P48246 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: EEF61A160175264A	FASTA	251	27,279
	10 20 30 40 50 60 MSGWRLLLTR PDEECAALAA SLGEAGVHSS SLPLLAIDPL EETPEQRTLM LDLDRYCAVV 70 80 90 100 110 120 VVSKPAARLG LERLDRYWPQ PPQQTWCSVG AATAAILEAY GLDVTYPEQG DDSEALLALP 130 140 150 160 170 180 AFQDSLRVHD PKVLIMRGEG GREFLAERLR GQGVQVDYLP LYRRRAPDYP AGELLARVRA 190 200 210 220 230 240 ERLNGLVVSS GQGLQNLYQL AAADWPEIGR LPLFVPSPRV AEMARELGAQ RVIDCRGASA 250 PALLAALTSA A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Pseudomonas aeruginosa homologs of hemC and hemD are linked to the gene encoding the regulator of mucoidy AlgR." Mohr C.D., Sonsteby S.K., Deretic V. Mol. Gen. Genet. 242:177-184(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M74844 Genomic DNA. Translation: AAA18908.1.
AE004091 Genomic DNA. Translation: AAG08644.1.

PIR

A82989.
S41587.

RefSeq

NP_253946.1. NC_002516.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4ES6	X-ray	2.22	A	1-251	[»]

ProteinModelPortal

P48246.

SMR

P48246. Positions 3-237.

ModBase

Search...

Protein-protein interaction databases

STRING

208964.PA5259.

Protocols and materials databases

DNASU

879724.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

879724.

KEGG

pae:PA5259.

PATRIC

19845371. VBIPseAer58763_5512.

Organism-specific databases

PseudoCAP

PA5259.

Phylogenomic databases

eggNOG

COG1587.

HOGENOM

HOG000247992.

KO

K01719.

OMA

YCECYQR.

ProtClustDB

PRK05752.

Enzyme and pathway databases

UniPathway

UPA00251; UER00320.

Family and domain databases

InterPro

IPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]

Pfam

PF02602. HEM4. 1 hit.
[Graphical view]

SUPFAM

SSF69618. HEM4_synth. 1 hit.

ProtoNet

Search...

Entry information

Entry name

HEM4_PSEAE

Accession

Primary (citable) accession number: P48246

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	December 8, 2000
Last modified:	May 1, 2013
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program