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Protein

Uroporphyrinogen-III synthase

Gene

hemD

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.By similarity

Catalytic activityi

Hydroxymethylbilane = uroporphyrinogen III + H2O.

Pathwayi

GO - Molecular functioni

  1. uroporphyrinogen-III synthase activity Source: PseudoCAP

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Porphyrin biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00251; UER00320.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III synthase (EC:4.2.1.75)
Short name:
UROS
Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase
Gene namesi
Name:hemD
Ordered Locus Names:PA5259
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA5259.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Uroporphyrinogen-III synthasePRO_0000135245Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi208964.PA5259.

Structurei

Secondary structure

1
251
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 84Combined sources
Helixi12 – 2413Combined sources
Beta strandi28 – 314Combined sources
Beta strandi36 – 394Combined sources
Helixi44 – 518Combined sources
Helixi53 – 553Combined sources
Beta strandi57 – 615Combined sources
Helixi64 – 7714Combined sources
Beta strandi85 – 906Combined sources
Helixi91 – 10010Combined sources
Helixi113 – 1175Combined sources
Helixi120 – 1256Combined sources
Beta strandi128 – 1303Combined sources
Beta strandi132 – 1376Combined sources
Helixi144 – 1518Combined sources
Beta strandi155 – 1606Combined sources
Beta strandi162 – 1654Combined sources
Helixi173 – 1808Combined sources
Beta strandi185 – 1873Combined sources
Helixi191 – 20111Combined sources
Helixi202 – 2043Combined sources
Helixi205 – 2084Combined sources
Beta strandi213 – 2175Combined sources
Helixi218 – 2269Combined sources
Beta strandi230 – 2345Combined sources
Beta strandi236 – 2394Combined sources
Helixi240 – 24910Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4ES6X-ray2.22A1-251[»]
ProteinModelPortaliP48246.
SMRiP48246. Positions 3-237.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1587.
HOGENOMiHOG000247992.
InParanoidiP48246.
KOiK01719.
OMAiYCECYQR.
OrthoDBiEOG6RZB2F.
PhylomeDBiP48246.

Family and domain databases

InterProiIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamiPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMiSSF69618. SSF69618. 1 hit.

Sequencei

Sequence statusi: Complete.

P48246-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGWRLLLTR PDEECAALAA SLGEAGVHSS SLPLLAIDPL EETPEQRTLM
60 70 80 90 100
LDLDRYCAVV VVSKPAARLG LERLDRYWPQ PPQQTWCSVG AATAAILEAY
110 120 130 140 150
GLDVTYPEQG DDSEALLALP AFQDSLRVHD PKVLIMRGEG GREFLAERLR
160 170 180 190 200
GQGVQVDYLP LYRRRAPDYP AGELLARVRA ERLNGLVVSS GQGLQNLYQL
210 220 230 240 250
AAADWPEIGR LPLFVPSPRV AEMARELGAQ RVIDCRGASA PALLAALTSA

A
Length:251
Mass (Da):27,279
Last modified:December 7, 2000 - v2
Checksum:iEEF61A160175264A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti195 – 2039QNLYQLAAA → PKSVSVGGS in AAA18908 (PubMed:8159168).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74844 Genomic DNA. Translation: AAA18908.1.
AE004091 Genomic DNA. Translation: AAG08644.1.
PIRiA82989.
S41587.
RefSeqiNP_253946.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG08644; AAG08644; PA5259.
GeneIDi879724.
KEGGipae:PA5259.
PATRICi19845371. VBIPseAer58763_5512.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74844 Genomic DNA. Translation: AAA18908.1.
AE004091 Genomic DNA. Translation: AAG08644.1.
PIRiA82989.
S41587.
RefSeqiNP_253946.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4ES6X-ray2.22A1-251[»]
ProteinModelPortaliP48246.
SMRiP48246. Positions 3-237.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA5259.

Protocols and materials databases

DNASUi879724.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08644; AAG08644; PA5259.
GeneIDi879724.
KEGGipae:PA5259.
PATRICi19845371. VBIPseAer58763_5512.

Organism-specific databases

PseudoCAPiPA5259.

Phylogenomic databases

eggNOGiCOG1587.
HOGENOMiHOG000247992.
InParanoidiP48246.
KOiK01719.
OMAiYCECYQR.
OrthoDBiEOG6RZB2F.
PhylomeDBiP48246.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00320.

Family and domain databases

InterProiIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamiPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMiSSF69618. SSF69618. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Pseudomonas aeruginosa homologs of hemC and hemD are linked to the gene encoding the regulator of mucoidy AlgR."
    Mohr C.D., Sonsteby S.K., Deretic V.
    Mol. Gen. Genet. 242:177-184(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  3. "The AEROPATH project targeting Pseudomonas aeruginosa: crystallographic studies for assessment of potential targets in early-stage drug discovery."
    Moynie L., Schnell R., McMahon S.A., Sandalova T., Boulkerou W.A., Schmidberger J.W., Alphey M., Cukier C., Duthie F., Kopec J., Liu H., Jacewicz A., Hunter W.N., Naismith J.H., Schneider G.
    Acta Crystallogr. F 69:25-34(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.22 ANGSTROMS) OF APOPROTEIN, SUBUNIT.

Entry informationi

Entry nameiHEM4_PSEAE
AccessioniPrimary (citable) accession number: P48246
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1996
Last sequence update: December 7, 2000
Last modified: January 6, 2015
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.