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P48246 (HEM4_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Uroporphyrinogen-III synthase

Short name=UROS
EC=4.2.1.75
Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase
Gene names
Name:hemD
Ordered Locus Names:PA5259
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length251 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III By similarity.

Catalytic activity

Hydroxymethylbilane = uroporphyrinogen III + H2O.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.

Subunit structure

Monomer. Ref.3

Sequence similarities

Belongs to the uroporphyrinogen-III synthase family.

Ontologies

Keywords
   Biological processPorphyrin biosynthesis
   Molecular functionLyase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionuroporphyrinogen-III synthase activity

Inferred from sequence or structural similarity PubMed 2557837. Source: PseudoCAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 251251Uroporphyrinogen-III synthase
PRO_0000135245

Experimental info

Sequence conflict195 – 2039QNLYQLAAA → PKSVSVGGS in AAA18908. Ref.1

Secondary structure

.................................................. 251
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P48246 [UniParc].

Last modified December 8, 2000. Version 2.
Checksum: EEF61A160175264A

FASTA25127,279
        10         20         30         40         50         60 
MSGWRLLLTR PDEECAALAA SLGEAGVHSS SLPLLAIDPL EETPEQRTLM LDLDRYCAVV 

        70         80         90        100        110        120 
VVSKPAARLG LERLDRYWPQ PPQQTWCSVG AATAAILEAY GLDVTYPEQG DDSEALLALP 

       130        140        150        160        170        180 
AFQDSLRVHD PKVLIMRGEG GREFLAERLR GQGVQVDYLP LYRRRAPDYP AGELLARVRA 

       190        200        210        220        230        240 
ERLNGLVVSS GQGLQNLYQL AAADWPEIGR LPLFVPSPRV AEMARELGAQ RVIDCRGASA 

       250 
PALLAALTSA A 

« Hide

References

« Hide 'large scale' references
[1]"The Pseudomonas aeruginosa homologs of hemC and hemD are linked to the gene encoding the regulator of mucoidy AlgR."
Mohr C.D., Sonsteby S.K., Deretic V.
Mol. Gen. Genet. 242:177-184(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]"The AEROPATH project targeting Pseudomonas aeruginosa: crystallographic studies for assessment of potential targets in early-stage drug discovery."
Moynie L., Schnell R., McMahon S.A., Sandalova T., Boulkerou W.A., Schmidberger J.W., Alphey M., Cukier C., Duthie F., Kopec J., Liu H., Jacewicz A., Hunter W.N., Naismith J.H., Schneider G.
Acta Crystallogr. F 69:25-34(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.22 ANGSTROMS) OF APOPROTEIN, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M74844 Genomic DNA. Translation: AAA18908.1.
AE004091 Genomic DNA. Translation: AAG08644.1.
PIRA82989.
S41587.
RefSeqNP_253946.1. NC_002516.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4ES6X-ray2.22A1-251[»]
ProteinModelPortalP48246.
SMRP48246. Positions 3-237.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208964.PA5259.

Protocols and materials databases

DNASU879724.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID879724.
KEGGpae:PA5259.
PATRIC19845371. VBIPseAer58763_5512.

Organism-specific databases

PseudoCAPPA5259.

Phylogenomic databases

eggNOGCOG1587.
HOGENOMHOG000247992.
KOK01719.
OMAYCECYQR.
OrthoDBEOG6RZB2F.
ProtClustDBPRK05752.

Enzyme and pathway databases

UniPathwayUPA00251; UER00320.

Family and domain databases

InterProIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMSSF69618. SSF69618. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHEM4_PSEAE
AccessionPrimary (citable) accession number: P48246
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 8, 2000
Last modified: April 16, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways