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Protein

Uroporphyrinogen-III synthase

Gene

hemD

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.By similarity

Catalytic activityi

Hydroxymethylbilane = uroporphyrinogen III + H2O.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (hemB)
  2. Porphobilinogen deaminase (hemC)
  3. Uroporphyrinogen-III synthase (hemD)
  4. Uroporphyrinogen decarboxylase (hemE)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

  • uroporphyrinogen-III synthase activity Source: PseudoCAP

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Porphyrin biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00251; UER00320.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III synthase (EC:4.2.1.75)
Short name:
UROS
Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase
Gene namesi
Name:hemD
Ordered Locus Names:PA5259
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA5259.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001352451 – 251Uroporphyrinogen-III synthaseAdd BLAST251

Proteomic databases

PaxDbiP48246.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi208964.PA5259.

Structurei

Secondary structure

1251
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 8Combined sources4
Helixi12 – 24Combined sources13
Beta strandi28 – 31Combined sources4
Beta strandi36 – 39Combined sources4
Helixi44 – 51Combined sources8
Helixi53 – 55Combined sources3
Beta strandi57 – 61Combined sources5
Helixi64 – 77Combined sources14
Beta strandi85 – 90Combined sources6
Helixi91 – 100Combined sources10
Helixi113 – 117Combined sources5
Helixi120 – 125Combined sources6
Beta strandi128 – 130Combined sources3
Beta strandi132 – 137Combined sources6
Helixi144 – 151Combined sources8
Beta strandi155 – 160Combined sources6
Beta strandi162 – 165Combined sources4
Helixi173 – 180Combined sources8
Beta strandi185 – 187Combined sources3
Helixi191 – 201Combined sources11
Helixi202 – 204Combined sources3
Helixi205 – 208Combined sources4
Beta strandi213 – 217Combined sources5
Helixi218 – 226Combined sources9
Beta strandi230 – 234Combined sources5
Beta strandi236 – 239Combined sources4
Helixi240 – 249Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ES6X-ray2.22A1-251[»]
ProteinModelPortaliP48246.
SMRiP48246.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG41076E6. Bacteria.
COG1587. LUCA.
HOGENOMiHOG000247992.
InParanoidiP48246.
KOiK01719.
OMAiGWFTVGE.
PhylomeDBiP48246.

Family and domain databases

CDDicd06578. HemD. 1 hit.
InterProiIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamiPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMiSSF69618. SSF69618. 1 hit.

Sequencei

Sequence statusi: Complete.

P48246-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGWRLLLTR PDEECAALAA SLGEAGVHSS SLPLLAIDPL EETPEQRTLM
60 70 80 90 100
LDLDRYCAVV VVSKPAARLG LERLDRYWPQ PPQQTWCSVG AATAAILEAY
110 120 130 140 150
GLDVTYPEQG DDSEALLALP AFQDSLRVHD PKVLIMRGEG GREFLAERLR
160 170 180 190 200
GQGVQVDYLP LYRRRAPDYP AGELLARVRA ERLNGLVVSS GQGLQNLYQL
210 220 230 240 250
AAADWPEIGR LPLFVPSPRV AEMARELGAQ RVIDCRGASA PALLAALTSA

A
Length:251
Mass (Da):27,279
Last modified:December 8, 2000 - v2
Checksum:iEEF61A160175264A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti195 – 203QNLYQLAAA → PKSVSVGGS in AAA18908 (PubMed:8159168).Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74844 Genomic DNA. Translation: AAA18908.1.
AE004091 Genomic DNA. Translation: AAG08644.1.
PIRiA82989.
S41587.
RefSeqiNP_253946.1. NC_002516.2.
WP_003114032.1. NZ_ASJY01000817.1.

Genome annotation databases

EnsemblBacteriaiAAG08644; AAG08644; PA5259.
GeneIDi879724.
KEGGipae:PA5259.
PATRICi19845371. VBIPseAer58763_5512.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74844 Genomic DNA. Translation: AAA18908.1.
AE004091 Genomic DNA. Translation: AAG08644.1.
PIRiA82989.
S41587.
RefSeqiNP_253946.1. NC_002516.2.
WP_003114032.1. NZ_ASJY01000817.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ES6X-ray2.22A1-251[»]
ProteinModelPortaliP48246.
SMRiP48246.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA5259.

Proteomic databases

PaxDbiP48246.

Protocols and materials databases

DNASUi879724.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08644; AAG08644; PA5259.
GeneIDi879724.
KEGGipae:PA5259.
PATRICi19845371. VBIPseAer58763_5512.

Organism-specific databases

PseudoCAPiPA5259.

Phylogenomic databases

eggNOGiENOG41076E6. Bacteria.
COG1587. LUCA.
HOGENOMiHOG000247992.
InParanoidiP48246.
KOiK01719.
OMAiGWFTVGE.
PhylomeDBiP48246.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00320.

Family and domain databases

CDDicd06578. HemD. 1 hit.
InterProiIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamiPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMiSSF69618. SSF69618. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHEM4_PSEAE
AccessioniPrimary (citable) accession number: P48246
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 8, 2000
Last modified: November 2, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.