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P48246

- HEM4_PSEAE

UniProt

P48246 - HEM4_PSEAE

Protein

Uroporphyrinogen-III synthase

Gene

hemD

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 2 (08 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.By similarity

    Catalytic activityi

    Hydroxymethylbilane = uroporphyrinogen III + H2O.

    Pathwayi

    GO - Molecular functioni

    1. uroporphyrinogen-III synthase activity Source: PseudoCAP

    GO - Biological processi

    1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Porphyrin biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00251; UER00320.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Uroporphyrinogen-III synthase (EC:4.2.1.75)
    Short name:
    UROS
    Alternative name(s):
    Hydroxymethylbilane hydrolyase [cyclizing]
    Uroporphyrinogen-III cosynthase
    Gene namesi
    Name:hemD
    Ordered Locus Names:PA5259
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA5259.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 251251Uroporphyrinogen-III synthasePRO_0000135245Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi208964.PA5259.

    Structurei

    Secondary structure

    1
    251
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 84
    Helixi12 – 2413
    Beta strandi28 – 314
    Beta strandi36 – 394
    Helixi44 – 518
    Helixi53 – 553
    Beta strandi57 – 615
    Helixi64 – 7714
    Beta strandi85 – 906
    Helixi91 – 10010
    Helixi113 – 1175
    Helixi120 – 1256
    Beta strandi128 – 1303
    Beta strandi132 – 1376
    Helixi144 – 1518
    Beta strandi155 – 1606
    Beta strandi162 – 1654
    Helixi173 – 1808
    Beta strandi185 – 1873
    Helixi191 – 20111
    Helixi202 – 2043
    Helixi205 – 2084
    Beta strandi213 – 2175
    Helixi218 – 2269
    Beta strandi230 – 2345
    Beta strandi236 – 2394
    Helixi240 – 24910

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4ES6X-ray2.22A1-251[»]
    ProteinModelPortaliP48246.
    SMRiP48246. Positions 3-237.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1587.
    HOGENOMiHOG000247992.
    KOiK01719.
    OMAiYCECYQR.
    OrthoDBiEOG6RZB2F.
    PhylomeDBiP48246.

    Family and domain databases

    InterProiIPR003754. 4pyrrol_synth_uPrphyn_synth.
    [Graphical view]
    PfamiPF02602. HEM4. 1 hit.
    [Graphical view]
    SUPFAMiSSF69618. SSF69618. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P48246-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGWRLLLTR PDEECAALAA SLGEAGVHSS SLPLLAIDPL EETPEQRTLM    50
    LDLDRYCAVV VVSKPAARLG LERLDRYWPQ PPQQTWCSVG AATAAILEAY 100
    GLDVTYPEQG DDSEALLALP AFQDSLRVHD PKVLIMRGEG GREFLAERLR 150
    GQGVQVDYLP LYRRRAPDYP AGELLARVRA ERLNGLVVSS GQGLQNLYQL 200
    AAADWPEIGR LPLFVPSPRV AEMARELGAQ RVIDCRGASA PALLAALTSA 250
    A 251
    Length:251
    Mass (Da):27,279
    Last modified:December 8, 2000 - v2
    Checksum:iEEF61A160175264A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti195 – 2039QNLYQLAAA → PKSVSVGGS in AAA18908. (PubMed:8159168)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74844 Genomic DNA. Translation: AAA18908.1.
    AE004091 Genomic DNA. Translation: AAG08644.1.
    PIRiA82989.
    S41587.
    RefSeqiNP_253946.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG08644; AAG08644; PA5259.
    GeneIDi879724.
    KEGGipae:PA5259.
    PATRICi19845371. VBIPseAer58763_5512.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74844 Genomic DNA. Translation: AAA18908.1 .
    AE004091 Genomic DNA. Translation: AAG08644.1 .
    PIRi A82989.
    S41587.
    RefSeqi NP_253946.1. NC_002516.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4ES6 X-ray 2.22 A 1-251 [» ]
    ProteinModelPortali P48246.
    SMRi P48246. Positions 3-237.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA5259.

    Protocols and materials databases

    DNASUi 879724.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG08644 ; AAG08644 ; PA5259 .
    GeneIDi 879724.
    KEGGi pae:PA5259.
    PATRICi 19845371. VBIPseAer58763_5512.

    Organism-specific databases

    PseudoCAPi PA5259.

    Phylogenomic databases

    eggNOGi COG1587.
    HOGENOMi HOG000247992.
    KOi K01719.
    OMAi YCECYQR.
    OrthoDBi EOG6RZB2F.
    PhylomeDBi P48246.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00320 .

    Family and domain databases

    InterProi IPR003754. 4pyrrol_synth_uPrphyn_synth.
    [Graphical view ]
    Pfami PF02602. HEM4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69618. SSF69618. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The Pseudomonas aeruginosa homologs of hemC and hemD are linked to the gene encoding the regulator of mucoidy AlgR."
      Mohr C.D., Sonsteby S.K., Deretic V.
      Mol. Gen. Genet. 242:177-184(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    3. "The AEROPATH project targeting Pseudomonas aeruginosa: crystallographic studies for assessment of potential targets in early-stage drug discovery."
      Moynie L., Schnell R., McMahon S.A., Sandalova T., Boulkerou W.A., Schmidberger J.W., Alphey M., Cukier C., Duthie F., Kopec J., Liu H., Jacewicz A., Hunter W.N., Naismith J.H., Schneider G.
      Acta Crystallogr. F 69:25-34(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.22 ANGSTROMS) OF APOPROTEIN, SUBUNIT.

    Entry informationi

    Entry nameiHEM4_PSEAE
    AccessioniPrimary (citable) accession number: P48246
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: December 8, 2000
    Last modified: October 1, 2014
    This is version 83 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3