Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine palmitoyltransferase 2

Gene

LCB2

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2.

Cofactori

Pathway:isphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00222.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine palmitoyltransferase 2 (EC:2.3.1.50)
Short name:
SPT 2
Alternative name(s):
Long chain base biosynthesis protein 2
Gene namesi
Name:LCB2
Ordered Locus Names:KLLA0D02134g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598 Componenti: Chromosome D

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei61 – 8121HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 562562Serine palmitoyltransferase 2PRO_0000163860Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei365 – 3651N6-(pyridoxal phosphate)lysineBy similarity

Interactioni

Protein-protein interaction databases

STRINGi284590.XP_453163.1.

Structurei

3D structure databases

ProteinModelPortaliP48241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0156.
HOGENOMiHOG000206826.
InParanoidiP48241.
KOiK00654.
OMAiEQIVTSM.
OrthoDBiEOG7XPZG0.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISISSTRVP LIPPEDIPLE DKKENEFGQL TSEEYLYQSK SRDGKALQDP
60 70 80 90 100
ILDAPAYHVS LITYLNYLIL IILGHIHDFL GLTFQKEKHK DIMEQDGLAP
110 120 130 140 150
WFSTFESFYV RRLKQRIDDC FSRPTTGVPG RFIRCLDRVS HNLNDYFTYP
160 170 180 190 200
GTTSMCLNLS SYNYLGFAQS EGQCTTAALE ATDKYGVYSG GPRTRIGTTD
210 220 230 240 250
LHVMTEKYVA QFVGKEDAIL FSMGYGTNAN FFNSFLDSKC LVISDSLNHT
260 270 280 290 300
SIRTGVRLSG AAVKTFKHND MRALEKLIRE QIVQGQSKTH RPWKKIIICV
310 320 330 340 350
EGLYSMEGTM ANLPKLVELK KKYKCYLFVD EAHSIGAMGP SGRGVCDFFG
360 370 380 390 400
IPCSDIDIMM GTLTKSFGAA GGYIAADKWI IDRFRLDLTT PHYGEPTPAP
410 420 430 440 450
VLAQIASSLK TITGDINPGE GQERLQRIAF NARYLRLALQ RLGFIVYGIA
460 470 480 490 500
DSPVIPMLLY APSKMPAFSR MMLQRKIAVV VVAYPATPLI ESRVRFCVSA
510 520 530 540 550
ALTKEDIDYL LQHINEVGDK LFLKVSSGKA GGSLDGKPPR WNIDEVIKRT
560
PTDCKDDSFF RI
Length:562
Mass (Da):62,937
Last modified:September 27, 2004 - v2
Checksum:iA23388A0BB681B1D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti191 – 1911G → V in AAC49535 (PubMed:8921873).Curated
Sequence conflicti492 – 4921S → L in AAC49535 (PubMed:8921873).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15646 Genomic DNA. Translation: AAC49535.1.
CR382124 Genomic DNA. Translation: CAH00259.1.
PIRiJC5182.
RefSeqiXP_453163.1. XM_453163.1.

Genome annotation databases

GeneIDi2892894.
KEGGikla:KLLA0D02134g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15646 Genomic DNA. Translation: AAC49535.1.
CR382124 Genomic DNA. Translation: CAH00259.1.
PIRiJC5182.
RefSeqiXP_453163.1. XM_453163.1.

3D structure databases

ProteinModelPortaliP48241.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284590.XP_453163.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2892894.
KEGGikla:KLLA0D02134g.

Phylogenomic databases

eggNOGiCOG0156.
HOGENOMiHOG000206826.
InParanoidiP48241.
KOiK00654.
OMAiEQIVTSM.
OrthoDBiEOG7XPZG0.

Enzyme and pathway databases

UniPathwayiUPA00222.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sphingolipid synthesis: identification and characterization of mammalian cDNAs encoding the Lcb2 subunit of serine palmitoyltransferase."
    Nagiec M.M., Lester R.L., Dickson R.C.
    Gene 177:237-241(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96263 / JA6.
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Entry informationi

Entry nameiLCB2_KLULA
AccessioniPrimary (citable) accession number: P48241
Secondary accession number(s): Q6CSC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: September 27, 2004
Last modified: July 22, 2015
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.