Exosome complex component MTR3 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P48240 (MTR3_YEAST)

Last modified June 16, 2009. Version 65. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Exosome complex component MTR3
Alternative name(s):
mRNA transport regulator 3

Gene names

Name:	MTR3
Ordered Locus Names:	YGR158C
ORF Names:	G6676

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Component of the exosome 3'->5' exoribonuclease complex but does not have exonuclease activity. As part of the exosome, required for the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA and for mRNA decay. Ref.3 Ref.4 Ref.8
Subunit structure	Component of the exosome multienzyme ribonuclease complex composed of 10 core proteins: the ring-forming non-catalytic subunits CSL4, MTR3, RRP4, RRP40, RRP42, RRP43, RRP45, RRP46 and SKI6/RRP41, and the catalytic subunit DIS3/RRP44. In the nucleus, exosome core is associated with at least RRP6 and LRP1/RRP47. Exosome nine subunit ring structure interacts with SKI7. Ref.8 Ref.7
Subcellular location	Cytoplasm. Nucleus › nucleolus. Ref.3 Ref.5
Miscellaneous	Present with 7380 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the RNase PH family.
Caution	According to Ref.8 and Ref.7, only DIS3/RRP44 subunit of the exosome core has exonuclease activity.

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Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm Exosome Nucleus
Ligand	RNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA fragment catabolic process Ref.4 Inferred from mutant phenotype. Source: SGD U4 snRNA 3'-end processing Inferred from mutant phenotype. Source: SGD exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Ref.4 Inferred from mutant phenotype. Source: SGD nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay Ref.4 Inferred by curator. Source: SGD nuclear-transcribed mRNA catabolic process, non-stop decay Ref.4 Inferred by curator. Source: SGD snoRNA 3'-end processing Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasmic exosome (RNase complex) Ref.4 Inferred from direct assay. Source: SGD nuclear exosome (RNase complex) Ref.4 Inferred from direct assay. Source: SGD nucleolus Ref.3 Inferred from direct assay. Source: SGD
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q06137	1	EBI-1749,EBI-33827

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 250

250

Exosome complex component MTR3

PRO_0000139979

Regions

Compositional bias

216 – 222

Asp/Glu-rich (acidic)

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Sequences

Sequence

Length

Mass (Da)

Tools

P48240-1 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 2FA07ABB4A1115E0
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FASTA

250

27,577

        10         20         30         40         50         60 
MNVQDRRRLL GPAAAKPMAF SNTTTHVPEK KSTDLTPKGN ESEQELSLHT GFIENCNGSA 

        70         80         90        100        110        120 
LVEARSLGHQ TSLITAVYGP RSIRGSFTSQ GTISIQLKNG LLEKYNTNEL KEVSSFLMGI 

       130        140        150        160        170        180 
FNSVVNLSRY PKSGIDIFVY LTYDKDLTNN PQDDDSQSKM MSSQISSLIP HCITSITLAL 

       190        200        210        220        230        240 
ADAGIELVDM AGAGEANGTV VSFIKNGEEI VGFWKDDGDD EDLLECLDRC KEQYNRYRDL 

       250 
MISCLMNQET

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 27 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals MOL1, NAT2, RPL30B, RSR1, CYS4, PEM1/CHO2, NSR1 genes and ten new open reading frames." Skala J., Nawrocki A., Goffeau A. Yeast 11:1421-1427(1995) [PubMed: 8585325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Mutations in nucleolar proteins lead to nucleolar accumulation of polyA+ RNA in Saccharomyces cerevisiae." Kadowaki T., Schneiter R., Hitomi M., Tartakoff A.M. Mol. Biol. Cell 6:1103-1110(1995) [PubMed: 8534909] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"The yeast exosome and human PM-Scl are related complexes of 3'-->5' exonucleases." Allmang C., Petfalski E., Podtelejnikov A., Mann M., Tollervey D., Mitchell P. Genes Dev. 13:2148-2158(1999) [PubMed: 10465791] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE EXOSOME COMPLEX BY MASS SPECTROMETRY.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Reconstitution, activities, and structure of the eukaryotic RNA exosome." Liu Q., Greimann J.C., Lima C.D. Cell 127:1223-1237(2006) [PubMed: 17174896] [Abstract] Cited for: LACK OF EXONUCLEASE ACTIVITY, SUBUNIT.
[8]	"A single subunit, Dis3, is essentially responsible for yeast exosome core activity." Dziembowski A., Lorentzen E., Conti E., Seraphin B. Nat. Struct. Mol. Biol. 14:15-22(2007) [PubMed: 17173052] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION OF THE EXOSOME WITH RRP6 AND SKI7, SUBUNIT.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	X85807 Genomic DNA. Translation: CAA59815.1. S80548 Genomic DNA. Translation: AAB35850.1. Z72943 Genomic DNA. Translation: CAA97172.1.
PIR	S58362.
RefSeq	NP_011674.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:931N.
IntAct	P48240. 26 interactions.
Proteomic databases
PeptideAtlas	P48240.
Genome annotation databases
Ensembl	YGR158C. Saccharomyces cerevisiae. [Contig view]
GeneID	853062.
GenomeReviews	Gene locus YGR158C in contig Y13135_GR.
KEGG	sce:YGR158C.
NMPDR	fig\|4932.3.peg.2794.
Organism-specific databases
CYGD	YGR158c.
SGD	S000003390. MTR3.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P48240.
OMA	P48240. RYPKSGI.
Gene expression databases
GermOnline	YGR158C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001247. ExoRNase_PH_dom1. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973001.

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Entry information

Entry name

MTR3_YEAST

Accession

Primary (citable) accession number: P48240

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents