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Protein

Glutathione S-transferase omega-like 1

Gene

GTO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Active as '1-Cys' thiol transferase against beta-hydroxyethyl disulfide (HED), as dehydroascorbate reductase and as dimethylarsinic acid reductase, while not active against the standard GST substrate 1-chloro-2,4-dinitrobenzene (CDNB).1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate.1 Publication

Kineticsi

  1. KM=1.13 mM for reduced glutathione (GSH)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei31NucleophileBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    • glutathione metabolic process Source: SGD

    Keywordsi

    Molecular functionOxidoreductase, Transferase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30856-MONOMER
    SABIO-RKiP48239

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase omega-like 1 (EC:2.5.1.18)
    Alternative name(s):
    Glutathione-dependent dehydroascorbate reductase (EC:1.8.5.1)
    Gene namesi
    Name:GTO1
    Ordered Locus Names:YGR154C
    ORF Names:G6664
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome VII

    Organism-specific databases

    EuPathDBiFungiDB:YGR154C
    SGDiS000003386 GTO1

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Peroxisome

    Pathology & Biotechi

    Disruption phenotypei

    Null mutants display increased sensitivity to cadmium in the absence of GTT1 and GTT2. Null mutants also show growth defects on oleic acid-based medium, indicative of abnormal peroxisomal functions and altered expression of genes related to sulfur amino acid metabolism, and have low levels of reduced glutathione.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002028331 – 356Glutathione S-transferase omega-like 1Add BLAST356

    Proteomic databases

    PaxDbiP48239
    PRIDEiP48239

    Expressioni

    Inductioni

    By diamide and 1-chloro-2,4-dinitrobenzene (CDNB) in a transcriptional factors YAP1 and/or MSN2/4-dependent manner.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi33402, 75 interactors
    DIPiDIP-1867N
    IntActiP48239, 17 interactors
    MINTiP48239
    STRINGi4932.YGR154C

    Structurei

    3D structure databases

    ProteinModelPortaliP48239
    SMRiP48239
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Coiled coili214 – 257Sequence analysisAdd BLAST44

    Sequence similaritiesi

    Belongs to the GST superfamily. Omega family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    GeneTreeiENSGT00530000065151
    HOGENOMiHOG000245143
    InParanoidiP48239
    OMAiLGCPFAC
    OrthoDBiEOG092C3FEI

    Family and domain databases

    InterProiView protein in InterPro
    IPR036282 Glutathione-S-Trfase_C_sf
    IPR004045 Glutathione_S-Trfase_N
    IPR016639 GST_Omega/GSH
    IPR036249 Thioredoxin-like_sf
    PANTHERiPTHR32419 PTHR32419, 1 hit
    PfamiView protein in Pfam
    PF13409 GST_N_2, 1 hit
    PIRSFiPIRSF015753 GST, 1 hit
    SUPFAMiSSF47616 SSF47616, 1 hit
    SSF52833 SSF52833, 2 hits

    Sequencei

    Sequence statusi: Complete.

    P48239-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSVSYKGTIS KTHSVFKPEK GRYYIYGALG CPFTHRAILA RSLKKLEPVL
    60 70 80 90 100
    GLVLSHWQLD SKGARFLPAP HRPEKYKERF FTATGGIASA KLDESEELGD
    110 120 130 140 150
    VNNDSARLFV DGAFDPVENI SRLSELYYLN DPKYPGTKFT VPVLWDSKTR
    160 170 180 190 200
    KIVNNESGDI IRILNSGVFD EFIQSEETNV IDLVPHDLID EIDKNIKWVH
    210 220 230 240 250
    PKINLGVYKV GLAENGKIYE TEVKTLFENL QKMECVLKEN YKRLEEQFSG
    260 270 280 290 300
    NKQKILAKYF VLGQRLTEAD IRLYPSIIRF DVVYVQHFKC NLKTIRDGFP
    310 320 330 340 350
    YLHLWLINLY WNYAEFRFTT DFNHIKLFYI RMEVSRNKIN QFGIVPLGPK

    PDISRL
    Length:356
    Mass (Da):41,301
    Last modified:February 1, 1996 - v1
    Checksum:iB4639BD195CDF7F5
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X85807 Genomic DNA Translation: CAA59811.1
    Z72939 Genomic DNA Translation: CAA97168.1
    BK006941 Genomic DNA Translation: DAA08245.1
    PIRiS60444
    RefSeqiNP_011670.3, NM_001181283.3

    Genome annotation databases

    EnsemblFungiiYGR154C; YGR154C; YGR154C
    GeneIDi853058
    KEGGisce:YGR154C

    Similar proteinsi

    Entry informationi

    Entry nameiGTO1_YEAST
    AccessioniPrimary (citable) accession number: P48239
    Secondary accession number(s): D6VUT4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: March 28, 2018
    This is version 126 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health