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P48239 (GTO1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione S-transferase omega-like 1

EC=2.5.1.18
Alternative name(s):
Glutathione-dependent dehydroascorbate reductase
EC=1.8.5.1
Gene names
Name:GTO1
Ordered Locus Names:YGR154C
ORF Names:G6664
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Active as '1-Cys' thiol transferase against beta-hydroxyethyl disulfide (HED), as dehydroascorbate reductase and as dimethylarsinic acid reductase, while not active against the standard GST substrate 1-chloro-2,4-dinitrobenzene (CDNB). Ref.4

Catalytic activity

RX + glutathione = HX + R-S-glutathione. Ref.4

2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate. Ref.4

Subcellular location

Peroxisome Ref.5.

Induction

By diamide and 1-chloro-2,4-dinitrobenzene (CDNB) in a transcriptional factors YAP1 and/or MSN2/4-dependent manner. Ref.5

Disruption phenotype

Null mutants display increased sensitivity to cadmium in the absence of GTT1 and GTT2. Null mutants also show growth defects on oleic acid-based medium, indicative of abnormal peroxisomal functions and altered expression of genes related to sulfur amino acid metabolism, and have low levels of reduced glutathione. Ref.5

Sequence similarities

Belongs to the GST superfamily. Omega family.

Biophysicochemical properties

Kinetic parameters:

KM=1.13 mM for reduced glutathione (GSH) Ref.4

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 356356Glutathione S-transferase omega-like 1
PRO_0000202833

Regions

Coiled coil214 – 25744 Potential

Sites

Active site311Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
P48239 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: B4639BD195CDF7F5

FASTA35641,301
        10         20         30         40         50         60 
MSVSYKGTIS KTHSVFKPEK GRYYIYGALG CPFTHRAILA RSLKKLEPVL GLVLSHWQLD 

        70         80         90        100        110        120 
SKGARFLPAP HRPEKYKERF FTATGGIASA KLDESEELGD VNNDSARLFV DGAFDPVENI 

       130        140        150        160        170        180 
SRLSELYYLN DPKYPGTKFT VPVLWDSKTR KIVNNESGDI IRILNSGVFD EFIQSEETNV 

       190        200        210        220        230        240 
IDLVPHDLID EIDKNIKWVH PKINLGVYKV GLAENGKIYE TEVKTLFENL QKMECVLKEN 

       250        260        270        280        290        300 
YKRLEEQFSG NKQKILAKYF VLGQRLTEAD IRLYPSIIRF DVVYVQHFKC NLKTIRDGFP 

       310        320        330        340        350 
YLHLWLINLY WNYAEFRFTT DFNHIKLFYI RMEVSRNKIN QFGIVPLGPK PDISRL 

« Hide

References

« Hide 'large scale' references
[1]"The sequence of a 27 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals MOL1, NAT2, RPL30B, RSR1, CYS4, PEM1/CHO2, NSR1 genes and ten new open reading frames."
Skala J., Nawrocki A., Goffeau A.
Yeast 11:1421-1427(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Saccharomyces cerevisiae cells have three Omega class glutathione S-transferases acting as 1-Cys thiol transferases."
Garcera A., Barreto L., Piedrafita L., Tamarit J., Herrero E.
Biochem. J. 398:187-196(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
[5]"A peroxisomal glutathione transferase of Saccharomyces cerevisiae is functionally related to sulfur amino acid metabolism."
Barreto L., Garcera A., Jansson K., Sunnerhagen P., Herrero E.
Eukaryot. Cell 5:1748-1759(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X85807 Genomic DNA. Translation: CAA59811.1.
Z72939 Genomic DNA. Translation: CAA97168.1.
BK006941 Genomic DNA. Translation: DAA08245.1.
PIRS60444.
RefSeqNP_011670.3. NM_001181283.3.

3D structure databases

ProteinModelPortalP48239.
SMRP48239. Positions 16-353.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33402. 8 interactions.
DIPDIP-1867N.
IntActP48239. 1 interaction.
MINTMINT-392461.
STRING4932.YGR154C.

Proteomic databases

PaxDbP48239.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR154C; YGR154C; YGR154C.
GeneID853058.
KEGGsce:YGR154C.

Organism-specific databases

CYGDYGR154c.
SGDS000003386. GTO1.

Phylogenomic databases

eggNOGCOG0435.
GeneTreeENSGT00530000065151.
HOGENOMHOG000245143.
OMAGTISKTH.
OrthoDBEOG722JK6.

Enzyme and pathway databases

BioCycYEAST:G3O-30856-MONOMER.
SABIO-RKP48239.

Gene expression databases

GenevestigatorP48239.

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.40.30.10. 2 hits.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR016639. GST.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERPTHR32419. PTHR32419. 1 hit.
PfamPF13409. GST_N_2. 1 hit.
[Graphical view]
PIRSFPIRSF015753. GST. 1 hit.
SUPFAMSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 2 hits.
ProtoNetSearch...

Other

NextBio972989.

Entry information

Entry nameGTO1_YEAST
AccessionPrimary (citable) accession number: P48239
Secondary accession number(s): D6VUT4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families