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Protein

Tricalbin-2

Gene

TCB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in membrane trafficking.2 Publications

GO - Molecular functioni

  • lipid binding Source: SGD

GO - Biological processi

  • endoplasmic reticulum membrane organization Source: SGD
  • regulation of phosphatidylinositol dephosphorylation Source: SGD
Complete GO annotation...

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33116-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tricalbin-2
Gene namesi
Name:TCB2
Ordered Locus Names:YNL087W
ORF Names:N2250
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL087W.
SGDiS000005031. TCB2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9898CytoplasmicBy similarityAdd
BLAST
Transmembranei99 – 11921HelicalSequence analysisAdd
BLAST
Topological domaini120 – 1201ExtracellularBy similarity
Transmembranei121 – 14121HelicalSequence analysisAdd
BLAST
Topological domaini142 – 11781037CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • cellular bud Source: SGD
  • cortical endoplasmic reticulum Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11781178Tricalbin-2PRO_0000203444Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei991 – 9911PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP48231.
PeptideAtlasiP48231.

PTM databases

iPTMnetiP48231.

Interactioni

Subunit structurei

Interacts with TCB1 and TCB3 via its C-terminal domain.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SEM1O947422EBI-28779,EBI-31337

Protein-protein interaction databases

BioGridi35736. 39 interactions.
DIPiDIP-2709N.
IntActiP48231. 6 interactions.
MINTiMINT-1571986.

Structurei

3D structure databases

ProteinModelPortaliP48231.
SMRiP48231. Positions 376-504, 623-738, 954-1090.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini365 – 46399C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini637 – 73397C2 2PROSITE-ProRule annotationAdd
BLAST
Domaini970 – 1070101C2 3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili784 – 82138Sequence analysisAdd
BLAST

Domaini

The C2 domains show Ca2+-independent phospholipid binding. None of the C2 domains retains all 5 conserved Asp residues found in calcium-binding C2 domains.

Sequence similaritiesi

Belongs to the tricalbin family.Curated
Contains 3 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00730000113103.
HOGENOMiHOG000248700.
InParanoidiP48231.
OMAiDGFATEI.
OrthoDBiEOG72JWQH.

Family and domain databases

Gene3Di2.60.40.150. 4 hits.
InterProiIPR000008. C2_dom.
IPR017147. Tricalbin.
[Graphical view]
PfamiPF00168. C2. 4 hits.
[Graphical view]
PIRSFiPIRSF037232. Tricalbin. 1 hit.
PRINTSiPR00360. C2DOMAIN.
SMARTiSM00239. C2. 4 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 4 hits.
PROSITEiPS50004. C2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48231-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPNSSKTRT DQISSMPGIN EATKVESKNV VKDAVPIKSE VETNGTSIVR
60 70 80 90 100
EKQDPSYVGW KQVGGWEEKD ELTSEDLLVD VNKDTFLGNL LPDKFYGDWY
110 120 130 140 150
HEVAILIIAG LCSFVLGYFK FSLASVLIVM LTTGMLYRTS SKKYRESLRD
160 170 180 190 200
LAQKEQTVEK ITSDYESVEW LNTFLDKYWP IIEPSVSQQI VDGTNTALSE
210 220 230 240 250
NVAIPKFIKA IWLDQFTLGV KPPRIDAIKT FQNTKSDVVV MDVCLSFTPH
260 270 280 290 300
DMSDLDAKQC RNYVNSNVVL KAKIFGMDIP VSVADIFFQV FVRFRFQLMT
310 320 330 340 350
TLPLVETINI QLLEVPEVDF ISRLLGNSVF NWEILAIPGL MRLIQKMAFK
360 370 380 390 400
YLSPVLLPPF SLQLNIPQLL SKTGLPIGVL EIKVKNAHGL RKLVGMIKKT
410 420 430 440 450
VDPYLTFELS GKIVGKTKVF KNSANPVWNE SIYILLQSFT DPLTIAVYDK
460 470 480 490 500
RETLSDKKMG TVIFNLNKLH ANHYHKNEKV HFLRNSKPVG ELTFDLRFFP
510 520 530 540 550
TIEPKKLLNG DEEPLPDMNT GITKITIREL KGLDELSDKK FVFAELYVNA
560 570 580 590 600
ELVMTTKKEK RTAHLKWNSD YYSVVTDRRK TICRFVLKDQ SGKVISSSVQ
610 620 630 640 650
PLNHLIDRTE VNKEWIPLRN GKGELKVTTY WRPVDIDLGL KSVGYTTPIG
660 670 680 690 700
MLRVFINKAE NLRNPDSLGK ISPYAKVSVN GVARGRTNER IETLNPIWNQ
710 720 730 740 750
SIYVSVTSPL QKVSIDCFGI DTNGDDHNLG SLNIQTQNIY HKDNDDKYTI
760 770 780 790 800
FIDNAPRTGN LIGKKGVKGT VTYYLSFYPV VPVLSLEEAK EVDEINEKKD
810 820 830 840 850
KLEKQKSTLD DKNISKEEKE RIKKEEFRLT EKYDMYSYKM KLDLDELLQY
860 870 880 890 900
NAGVLGVTVL GGELPQPGLY VQTFFDSCGY AAITSAKNAI RTIKTGWSGD
910 920 930 940 950
FMIKELEWSV TTFRVTKTKD ANKAENFICE VNIPTIELVR NCYYKPSVLN
960 970 980 990 1000
LIGKKSAKLL VQVSWFPVTA TELPQSDLIT NSGDLKITAK SAENLIGVNK
1010 1020 1030 1040 1050
NGYSDPYVEF FLNEKSTSPF FKTAVQKKTL NPTWNESKTI EVSNRVNDYL
1060 1070 1080 1090 1100
TINVKDYEST NSNRSIGKAV VPLSTIDPES DTTFNIPLVG PKGEDGGVLH
1110 1120 1130 1140 1150
LEFEFEPRYT TNVVKREAGL GNFATKGLGT GIKAGSTVFA LGTNVVSTGL
1160 1170
GTIDKVKAGV FGGKKSTTTG DKKSEEKQ
Length:1,178
Mass (Da):132,509
Last modified:February 1, 1996 - v1
Checksum:i6597DE191C739F41
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti11 – 133DQI → EQV in strain: SK1.
Natural varianti121 – 1211F → S in strain: SK1. 1 Publication
Natural varianti528 – 5281R → G in strain: SK1. 1 Publication
Natural varianti691 – 6922IE → MK in strain: SK1.
Natural varianti1159 – 11591G → R in strain: SK1. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ115393 Genomic DNA. Translation: AAZ22521.1.
X89016 Genomic DNA. Translation: CAA61423.1.
Z71363 Genomic DNA. Translation: CAA95963.1.
BK006947 Genomic DNA. Translation: DAA10458.1.
PIRiS57535.
RefSeqiNP_014312.1. NM_001182925.1.

Genome annotation databases

EnsemblFungiiYNL087W; YNL087W; YNL087W.
GeneIDi855637.
KEGGisce:YNL087W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ115393 Genomic DNA. Translation: AAZ22521.1.
X89016 Genomic DNA. Translation: CAA61423.1.
Z71363 Genomic DNA. Translation: CAA95963.1.
BK006947 Genomic DNA. Translation: DAA10458.1.
PIRiS57535.
RefSeqiNP_014312.1. NM_001182925.1.

3D structure databases

ProteinModelPortaliP48231.
SMRiP48231. Positions 376-504, 623-738, 954-1090.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35736. 39 interactions.
DIPiDIP-2709N.
IntActiP48231. 6 interactions.
MINTiMINT-1571986.

PTM databases

iPTMnetiP48231.

Proteomic databases

MaxQBiP48231.
PeptideAtlasiP48231.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL087W; YNL087W; YNL087W.
GeneIDi855637.
KEGGisce:YNL087W.

Organism-specific databases

EuPathDBiFungiDB:YNL087W.
SGDiS000005031. TCB2.

Phylogenomic databases

GeneTreeiENSGT00730000113103.
HOGENOMiHOG000248700.
InParanoidiP48231.
OMAiDGFATEI.
OrthoDBiEOG72JWQH.

Enzyme and pathway databases

BioCyciYEAST:G3O-33116-MONOMER.

Miscellaneous databases

NextBioi979857.
PROiP48231.

Family and domain databases

Gene3Di2.60.40.150. 4 hits.
InterProiIPR000008. C2_dom.
IPR017147. Tricalbin.
[Graphical view]
PfamiPF00168. C2. 4 hits.
[Graphical view]
PIRSFiPIRSF037232. Tricalbin. 1 hit.
PRINTSiPR00360. C2DOMAIN.
SMARTiSM00239. C2. 4 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 4 hits.
PROSITEiPS50004. C2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Quantitative trait loci mapped to single-nucleotide resolution in yeast."
    Deutschbauer A.M., Davis R.W.
    Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS 11-GLU--VAL-13; SER-121; GLY-528; 691-MET-LYS-692 AND ARG-1159.
    Strain: SK1.
  2. "The sequence of a 17,933 bp segment of Saccharomyces cerevisiae chromosome XIV contains the RHO2, TOP2, MKT1 and END3 genes and five new open reading frames."
    Soler-Mira A., Saiz J.E., Ballesta J.P.G., Remacha M.A.
    Yeast 12:485-491(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "The tricalbin C2 domains: lipid-binding properties of a novel, synaptotagmin-like yeast protein family."
    Schulz T.A., Creutz C.E.
    Biochemistry 43:3987-3995(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, LIPID-BINDING.
  8. "Characterization of the yeast tricalbins: membrane-bound multi-C2-domain proteins that form complexes involved in membrane trafficking."
    Creutz C.E., Snyder S.L., Schulz T.A.
    Cell. Mol. Life Sci. 61:1208-1220(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TCB1 AND TCB3.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTCB2_YEAST
AccessioniPrimary (citable) accession number: P48231
Secondary accession number(s): D6W192, Q45TY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 11, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2630 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.