P48199 (CRP_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-reactive protein

Gene names

Name:	Crp
Synonyms:	Ptx1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells By similarity.
Cofactor	Binds 2 calcium ions per subunit By similarity.
Subunit structure	Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits. Two of the five chains form a dimer linked by two interchain disulfide bonds located in the C-terminal heptapeptide and specific to rat CRP.
Subcellular location	Secreted.
Tissue specificity	Found in plasma.
Post-translational modification	The last two cysteines are involved either in interchain disulfide bonds or in an intrachain bond.
Sequence similarities	Belongs to the pentaxin family. Contains 1 pentaxin domain.

Ontologies

Keywords
Biological process	Acute phase
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	acute-phase response Inferred from electronic annotation. Source: UniProtKB-KW complement activation, classical pathway Inferred from mutant phenotype. Source: RGD protein polymerization Inferred from direct assay Ref.1. Source: RGD response to hypoxia Inferred from expression pattern. Source: RGD response to lead ion Inferred from expression pattern. Source: RGD
Cellular component	extracellular space Inferred from direct assay Ref.1. Source: RGD
Molecular function	cholesterol binding Inferred from direct assay. Source: RGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein homodimerization activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	By similarity
	Chain	20 – 230	211	C-reactive protein		PRO_0000023532
Regions
	Domain	20 – 230	211	Pentaxin
Sites
	Metal binding	78	1	Calcium 1 By similarity
	Metal binding	155	1	Calcium 1 By similarity
	Metal binding	155	1	Calcium 2 By similarity
	Metal binding	156	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	157	1	Calcium 1 By similarity
	Metal binding	157	1	Calcium 2 By similarity
	Metal binding	167	1	Calcium 2 By similarity
Amino acid modifications
	Glycosylation	147	1	N-linked (GlcNAc...)		CAR_000154
	Disulfide bond	55 ↔ 114
	Disulfide bond	227 ↔ 228		In monomeric form
	Disulfide bond	227		Interchain; in polymeric form
	Disulfide bond	228		Interchain; in polymeric form

Sequences

	Sequence		Length	Mass (Da)
	P48199 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: D8CF6BFE72376309	FASTA	230	25,468
	10 20 30 40 50 60 MEKLLWCLLI TISFSQAFGH EDMSKQAFVF PGVSATAYVS LEAESKKPLE AFTVCLYAHA 70 80 90 100 110 120 DVSRSFSIFS YATKTSFNEI LLFWTRGQGF SIAVGGPEIL FSASEIPEVP THICATWESA 130 140 150 160 170 180 TGIVELWLDG KPRVRKSLQK GYIVGTNASI ILGQEQDSYG GGFDANQSLV GDIGDVNMWD 190 200 210 220 230 FVLSPEQINA VYVGRVFSPN VLNWRALKYE THGDVFIKPQ LWPLTDCCES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Derivation of the amino acid sequence of rat C-reactive protein from cDNA cloning with additional studies on the nature of its dimeric component." Rassouli M., Sambasivam H., Azadi P., Dell A., Morris H.R., Nagpurkar A., Mookerjea S., Murray R.K. J. Biol. Chem. 267:2947-2954(1992) [PubMed: 1737750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.
[3]	Lubec G., Kang S.U., Lubec S. Submitted (SEP-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 65-74 AND 196-205, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

No more Christmas pudding? - Issue 30 of January 2003

Cross-references

Sequence databases
EMBL GenBank DDBJ	M83176 mRNA. Translation: AAA40964.1. BC091157 mRNA. Translation: AAH91157.1.
IPI	IPI00188225.
PIR	A42579.
RefSeq	NP_058792.1. NM_017096.3.
UniGene	Rn.16463.
3D structure databases
ProteinModelPortal	P48199.
SMR	P48199. Positions 22-223.
ModBase	Search...
Protein-protein interaction databases
STRING	P48199.
PTM databases
GlycoSuiteDB	P48199.
Proteomic databases
PRIDE	P48199.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000000058; ENSRNOP00000000058; ENSRNOG00000000053.
GeneID	25419.
KEGG	rno:25419.
UCSC	NM_017096. rat.
Organism-specific databases
CTD	1401.
RGD	2411. Crp.
Phylogenomic databases
eggNOG	euNOG15062.
GeneTree	ENSGT00550000074240.
HOVERGEN	HBG005405.
OMA	CTSWESA.
OrthoDB	EOG4R23VS.
Gene expression databases
ArrayExpress	P48199.
Genevestigator	P48199.
GermOnline	ENSRNOG00000000053. Rattus norvegicus.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR013320. ConA-like_subgrp. IPR001759. Pentaxin. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF00354. Pentaxin. 1 hit. [Graphical view]
PRINTS	PR00895. PENTAXIN.
SMART	SM00159. PTX. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS00289. PENTAXIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	606575.

Entry information

Entry name

CRP_RAT

Accession

Primary (citable) accession number: P48199
Secondary accession number(s): Q5BK94

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	September 21, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program