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P48193

- 41_MOUSE

UniProt

P48193 - 41_MOUSE

Protein

Protein 4.1

Gene

Epb41

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (29 May 2007)
      Previous versions | rss
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    Functioni

    Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes By similarity.By similarity

    GO - Molecular functioni

    1. 1-phosphatidylinositol binding Source: Ensembl
    2. actin binding Source: MGI
    3. spectrin binding Source: MGI
    4. structural molecule activity Source: InterPro

    GO - Biological processi

    1. actin cytoskeleton organization Source: MGI
    2. cortical actin cytoskeleton organization Source: InterPro
    3. positive regulation of protein binding Source: MGI
    4. regulation of cell shape Source: MGI

    Keywords - Ligandi

    Actin-binding, Calmodulin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein 4.1
    Short name:
    P4.1
    Alternative name(s):
    4.1R
    Band 4.1
    Gene namesi
    Name:Epb41
    Synonyms:Epb4.1, Kiaa4056
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:95401. Epb4.1.

    Subcellular locationi

    Cytoplasmcytoskeleton By similarity. Cytoplasmcell cortex By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. actin cytoskeleton Source: MGI
    2. cortical cytoskeleton Source: MGI
    3. extrinsic component of membrane Source: InterPro
    4. Golgi apparatus Source: Ensembl
    5. membrane Source: MGI
    6. nucleus Source: UniProtKB-SubCell
    7. plasma membrane Source: Ensembl
    8. protein complex Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 858858Protein 4.1PRO_0000219391Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei14 – 141PhosphoserineBy similarity
    Modified residuei62 – 621PhosphothreonineBy similarity
    Modified residuei86 – 861PhosphoserineBy similarity
    Modified residuei87 – 871PhosphoserineBy similarity
    Modified residuei151 – 1511PhosphoserineBy similarity
    Modified residuei153 – 1531PhosphoserineBy similarity
    Modified residuei154 – 1541PhosphoserineBy similarity
    Modified residuei192 – 1921PhosphoserineBy similarity
    Modified residuei223 – 2231Phosphotyrosine1 Publication
    Modified residuei541 – 5411Phosphoserine1 Publication
    Modified residuei543 – 5431Phosphoserine2 Publications
    Modified residuei556 – 5561PhosphoserineBy similarity
    Modified residuei654 – 6541PhosphotyrosineBy similarity
    Modified residuei706 – 7061PhosphoserineBy similarity

    Post-translational modificationi

    O-glycosylated; contains N-acetylglucosamine side chains in the C-terminal domain.By similarity
    Phosphorylated at multiple sites by different protein kinases and each phosphorylation event selectively modulates the protein's functions.
    Phosphorylation on Tyr-654 reduces the ability of 4.1 to promote the assembly of the spectrin/actin/4.1 ternary complex.

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP48193.
    PaxDbiP48193.
    PRIDEiP48193.

    PTM databases

    PhosphoSiteiP48193.

    Expressioni

    Gene expression databases

    ArrayExpressiP48193.
    BgeeiP48193.
    CleanExiMM_EPB4.1.
    GenevestigatoriP48193.

    Interactioni

    Subunit structurei

    Binds with a high affinity to glycophorin and with lower affinity to band III protein. Associates with the nuclear mitotic apparatus. Binds calmodulin and DLG1 By similarity.By similarity

    Protein-protein interaction databases

    IntActiP48193. 3 interactions.
    MINTiMINT-149787.

    Structurei

    3D structure databases

    ProteinModelPortaliP48193.
    SMRiP48193. Positions 209-520.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini211 – 492282FERMPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni495 – 608114HydrophilicAdd
    BLAST
    Regioni609 – 70799Spectrin--actin-bindingAdd
    BLAST
    Regioni710 – 858149C-terminal (CTD)Add
    BLAST

    Sequence similaritiesi

    Contains 1 FERM domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG242913.
    GeneTreeiENSGT00750000117241.
    HOGENOMiHOG000228841.
    HOVERGENiHBG007777.
    KOiK06107.

    Family and domain databases

    Gene3Di1.20.80.10. 1 hit.
    2.30.29.30. 1 hit.
    InterProiIPR008379. Band_4.1_C.
    IPR019749. Band_41_domain.
    IPR019750. Band_41_fam.
    IPR021187. Band_41_protein.
    IPR000798. Ez/rad/moesin_like.
    IPR014847. FERM-adjacent.
    IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
    IPR019748. FERM_central.
    IPR019747. FERM_CS.
    IPR000299. FERM_domain.
    IPR018979. FERM_N.
    IPR018980. FERM_PH-like_C.
    IPR011993. PH_like_dom.
    IPR007477. SAB_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF05902. 4_1_CTD. 1 hit.
    PF08736. FA. 1 hit.
    PF09380. FERM_C. 1 hit.
    PF00373. FERM_M. 1 hit.
    PF09379. FERM_N. 1 hit.
    PF04382. SAB. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002304. Membrane_skeletal_4_1. 1 hit.
    PRINTSiPR00935. BAND41.
    PR00661. ERMFAMILY.
    SMARTiSM00295. B41. 1 hit.
    [Graphical view]
    SUPFAMiSSF47031. SSF47031. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEiPS00660. FERM_1. 1 hit.
    PS00661. FERM_2. 1 hit.
    PS50057. FERM_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Note: A number of isoforms are produced.

    Isoform 1 (identifier: P48193-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTTEKSLAAE AENSQHQQQK EEGEGATNSG QQETQLEEAS QAAAAEGSDQ    50
    GEQKLKASNG DTPTHEDLTK NKERTSESRG LSRLLSSFLK RPKSQVSEEE 100
    GREVESEKEK GEGGQKEIEL GNSLDEDIIL KAPIAAPEPE LKTDPSLDLH 150
    SLSSIETQPA QEEHREDPDS ETKEGEGIEE CSGTEVKEDP ESRAEREPEA 200
    SQKPVRRHRN MHCKVSLLDD TVYECVVEKH AKGQDLLKRV CEHLNLLEED 250
    YFGLALWDSA TSKTWLDSAK EIKKQVRGVP WNFTFNVKFY PPDPAQLTED 300
    ITRYYLCLQL RQDIVAGRLP CSFATLALLG SYTIQSELGD YDPELHGMDY 350
    VSDFKLAPNQ TKELEEKVME LHKSYRSMTP AQADLEFLEN AKKLSMYGVD 400
    LHKAKDLEGV DIILGVCSSG LLVYKDKLRI NRFPWPKVLK ISYKRSSFFI 450
    KIRPGEQEHY ESTIGFKLPS YRAAKKLWKV CVEHHTFFRL TSTDTIPKSK 500
    FLALGSKFRY SGRTQAQTRQ ASALIDRPAP HFERTASKRA SRSLDGAAAA 550
    ESTDRSPRPT SAPAIAQSQV TEGPGAPIKK TPKEAVKVEE KRGEEPAEPA 600
    EPEPTEAWKV EKTHTEVTVP TSNGDQTQKL AGKGEDLIRM RKKKRERLDG 650
    ENIYIRHSNL MLEDLDKSQE EIKKHHASIS ELKKNFMESV PEPRPSEWDK 700
    RLSTHSPFRT LNINGQVPTG DGPPLVKTQT VTISDTANAV KSEIPTKDVP 750
    IVHTETKTIT YEAAQTEDSN GDLDPGVLLT AQTITSETTS STTTTQITKT 800
    VKGGISETRI EKRIVITGDA DIDHDQVLVQ AIKEAKEQHP DMSVTKVVVH 850
    QETEISEE 858
    Length:858
    Mass (Da):95,911
    Last modified:May 29, 2007 - v2
    Checksum:i7CF1CD52D790D1FD
    GO
    Isoform 2 (identifier: P48193-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         610-663: Missing.

    Show »
    Length:804
    Mass (Da):89,664
    Checksum:iB4BCAD3F40CB7864
    GO

    Sequence cautioni

    The sequence BAD90280.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 33MTT → EPLKGREPRRARTRPGPARP GPCQVPVLCSP in AAH68138. (PubMed:15489334)Curated
    Sequence conflicti8 – 81A → V in AAA37123. (PubMed:8429050)Curated
    Sequence conflicti232 – 2332KG → NL in AAA37122. (PubMed:8429050)Curated
    Sequence conflicti443 – 4431Y → S in AAA37122. (PubMed:8429050)Curated
    Sequence conflicti576 – 5761A → R in AAA37122. (PubMed:8429050)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei610 – 66354Missing in isoform 2. 2 PublicationsVSP_012874Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L00919 mRNA. Translation: AAA37122.1.
    L00919 mRNA. Translation: AAA37123.1.
    AK220462 mRNA. Translation: BAD90280.1. Different initiation.
    AL607088, AL669981 Genomic DNA. Translation: CAM14971.1.
    AL669981, AL607088 Genomic DNA. Translation: CAM21329.1.
    BC068138 mRNA. Translation: AAH68138.1.
    BC079875 mRNA. Translation: AAH79875.1.
    CCDSiCCDS18717.1. [P48193-1]
    CCDS51317.1. [P48193-2]
    PIRiA46613.
    RefSeqiNP_001122078.1. NM_001128606.1. [P48193-2]
    NP_001122079.1. NM_001128607.1.
    NP_906273.3. NM_183428.3. [P48193-1]
    UniGeneiMm.30038.
    Mm.471627.

    Genome annotation databases

    EnsembliENSMUST00000030739; ENSMUSP00000030739; ENSMUSG00000028906. [P48193-1]
    ENSMUST00000054917; ENSMUSP00000060375; ENSMUSG00000028906. [P48193-2]
    ENSMUST00000084253; ENSMUSP00000081274; ENSMUSG00000028906. [P48193-2]
    ENSMUST00000105972; ENSMUSP00000101592; ENSMUSG00000028906. [P48193-1]
    ENSMUST00000105981; ENSMUSP00000101601; ENSMUSG00000028906. [P48193-1]
    GeneIDi269587.
    KEGGimmu:269587.
    UCSCiuc008vam.3. mouse. [P48193-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L00919 mRNA. Translation: AAA37122.1 .
    L00919 mRNA. Translation: AAA37123.1 .
    AK220462 mRNA. Translation: BAD90280.1 . Different initiation.
    AL607088 , AL669981 Genomic DNA. Translation: CAM14971.1 .
    AL669981 , AL607088 Genomic DNA. Translation: CAM21329.1 .
    BC068138 mRNA. Translation: AAH68138.1 .
    BC079875 mRNA. Translation: AAH79875.1 .
    CCDSi CCDS18717.1. [P48193-1 ]
    CCDS51317.1. [P48193-2 ]
    PIRi A46613.
    RefSeqi NP_001122078.1. NM_001128606.1. [P48193-2 ]
    NP_001122079.1. NM_001128607.1.
    NP_906273.3. NM_183428.3. [P48193-1 ]
    UniGenei Mm.30038.
    Mm.471627.

    3D structure databases

    ProteinModelPortali P48193.
    SMRi P48193. Positions 209-520.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P48193. 3 interactions.
    MINTi MINT-149787.

    PTM databases

    PhosphoSitei P48193.

    Proteomic databases

    MaxQBi P48193.
    PaxDbi P48193.
    PRIDEi P48193.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030739 ; ENSMUSP00000030739 ; ENSMUSG00000028906 . [P48193-1 ]
    ENSMUST00000054917 ; ENSMUSP00000060375 ; ENSMUSG00000028906 . [P48193-2 ]
    ENSMUST00000084253 ; ENSMUSP00000081274 ; ENSMUSG00000028906 . [P48193-2 ]
    ENSMUST00000105972 ; ENSMUSP00000101592 ; ENSMUSG00000028906 . [P48193-1 ]
    ENSMUST00000105981 ; ENSMUSP00000101601 ; ENSMUSG00000028906 . [P48193-1 ]
    GeneIDi 269587.
    KEGGi mmu:269587.
    UCSCi uc008vam.3. mouse. [P48193-1 ]

    Organism-specific databases

    CTDi 269587.
    MGIi MGI:95401. Epb4.1.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG242913.
    GeneTreei ENSGT00750000117241.
    HOGENOMi HOG000228841.
    HOVERGENi HBG007777.
    KOi K06107.

    Miscellaneous databases

    NextBioi 392903.
    PROi P48193.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P48193.
    Bgeei P48193.
    CleanExi MM_EPB4.1.
    Genevestigatori P48193.

    Family and domain databases

    Gene3Di 1.20.80.10. 1 hit.
    2.30.29.30. 1 hit.
    InterProi IPR008379. Band_4.1_C.
    IPR019749. Band_41_domain.
    IPR019750. Band_41_fam.
    IPR021187. Band_41_protein.
    IPR000798. Ez/rad/moesin_like.
    IPR014847. FERM-adjacent.
    IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
    IPR019748. FERM_central.
    IPR019747. FERM_CS.
    IPR000299. FERM_domain.
    IPR018979. FERM_N.
    IPR018980. FERM_PH-like_C.
    IPR011993. PH_like_dom.
    IPR007477. SAB_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF05902. 4_1_CTD. 1 hit.
    PF08736. FA. 1 hit.
    PF09380. FERM_C. 1 hit.
    PF00373. FERM_M. 1 hit.
    PF09379. FERM_N. 1 hit.
    PF04382. SAB. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002304. Membrane_skeletal_4_1. 1 hit.
    PRINTSi PR00935. BAND41.
    PR00661. ERMFAMILY.
    SMARTi SM00295. B41. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47031. SSF47031. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEi PS00660. FERM_1. 1 hit.
    PS00661. FERM_2. 1 hit.
    PS50057. FERM_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic structure of the locus encoding protein 4.1. Structural basis for complex combinational patterns of tissue-specific alternative RNA splicing."
      Huang J.-P., Tang C.-J.C., Kou G.-H., Marchesi V.T., Benz E.J. Jr., Tang T.K.
      J. Biol. Chem. 268:3758-3766(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING.
      Strain: BALB/c.
    2. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
      Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Fetal brain.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6.
      Tissue: Brain.
    5. "Properties of the C-terminal domain of 4.1 proteins."
      Scott C., Phillips G.W., Baines A.J.
      Eur. J. Biochem. 268:3709-3717(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 709-713, IDENTIFICATION BY MASS SPECTROMETRY, CHARACTERIZATION OF CARBOXY-TERMINAL DOMAIN.
    6. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
      Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
      J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Mast cell.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-541 AND SER-543, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    8. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-543, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry namei41_MOUSE
    AccessioniPrimary (citable) accession number: P48193
    Secondary accession number(s): A2A843
    , Q5DTQ8, Q68FF1, Q6NVF5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3