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Protein

Protein 4.1

Gene

Epb41

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes (By similarity).By similarity

GO - Molecular functioni

  1. 1-phosphatidylinositol binding Source: MGI
  2. actin binding Source: MGI
  3. spectrin binding Source: MGI
  4. structural molecule activity Source: InterPro

GO - Biological processi

  1. actin cytoskeleton organization Source: MGI
  2. cortical actin cytoskeleton organization Source: InterPro
  3. positive regulation of protein binding Source: MGI
  4. regulation of cell shape Source: MGI
Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein 4.1
Short name:
P4.1
Alternative name(s):
4.1R
Band 4.1
Gene namesi
Name:Epb41
Synonyms:Epb4.1, Kiaa4056
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:95401. Epb4.1.

Subcellular locationi

Cytoplasmcytoskeleton By similarity. Cytoplasmcell cortex By similarity. Nucleus By similarity

GO - Cellular componenti

  1. actin cytoskeleton Source: MGI
  2. cortical cytoskeleton Source: MGI
  3. extrinsic component of membrane Source: InterPro
  4. membrane Source: MGI
  5. nucleus Source: UniProtKB-SubCell
  6. protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 858858Protein 4.1PRO_0000219391Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphoserineBy similarity
Modified residuei62 – 621PhosphothreonineBy similarity
Modified residuei86 – 861PhosphoserineBy similarity
Modified residuei87 – 871PhosphoserineBy similarity
Modified residuei151 – 1511PhosphoserineBy similarity
Modified residuei153 – 1531PhosphoserineBy similarity
Modified residuei154 – 1541PhosphoserineBy similarity
Modified residuei192 – 1921PhosphoserineBy similarity
Modified residuei223 – 2231Phosphotyrosine1 Publication
Modified residuei541 – 5411Phosphoserine1 Publication
Modified residuei543 – 5431Phosphoserine2 Publications
Modified residuei556 – 5561PhosphoserineBy similarity
Modified residuei654 – 6541PhosphotyrosineBy similarity
Modified residuei706 – 7061PhosphoserineBy similarity

Post-translational modificationi

O-glycosylated; contains N-acetylglucosamine side chains in the C-terminal domain.By similarity
Phosphorylated at multiple sites by different protein kinases and each phosphorylation event selectively modulates the protein's functions.
Phosphorylation on Tyr-654 reduces the ability of 4.1 to promote the assembly of the spectrin/actin/4.1 ternary complex.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP48193.
PaxDbiP48193.
PRIDEiP48193.

PTM databases

PhosphoSiteiP48193.

Expressioni

Gene expression databases

BgeeiP48193.
CleanExiMM_EPB4.1.
ExpressionAtlasiP48193. baseline and differential.
GenevestigatoriP48193.

Interactioni

Subunit structurei

Binds with a high affinity to glycophorin and with lower affinity to band III protein. Associates with the nuclear mitotic apparatus. Binds calmodulin and DLG1 (By similarity).By similarity

Protein-protein interaction databases

IntActiP48193. 3 interactions.
MINTiMINT-149787.

Structurei

3D structure databases

ProteinModelPortaliP48193.
SMRiP48193. Positions 209-520.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini211 – 492282FERMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni495 – 608114HydrophilicAdd
BLAST
Regioni609 – 70799Spectrin--actin-bindingAdd
BLAST
Regioni710 – 858149C-terminal (CTD)Add
BLAST

Sequence similaritiesi

Contains 1 FERM domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG242913.
GeneTreeiENSGT00760000118823.
HOGENOMiHOG000228841.
HOVERGENiHBG007777.
InParanoidiP48193.
KOiK06107.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR008379. Band_4.1_C.
IPR019749. Band_41_domain.
IPR021187. Band_41_protein.
IPR000798. Ez/rad/moesin_like.
IPR014847. FERM-adjacent.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR011993. PH_like_dom.
IPR007477. SAB_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF05902. 4_1_CTD. 1 hit.
PF08736. FA. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF04382. SAB. 1 hit.
[Graphical view]
PIRSFiPIRSF002304. Membrane_skeletal_4_1. 1 hit.
PRINTSiPR00935. BAND41.
PR00661. ERMFAMILY.
SMARTiSM00295. B41. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00660. FERM_1. 1 hit.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Note: A number of isoforms are produced.

Isoform 1 (identifier: P48193-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTEKSLAAE AENSQHQQQK EEGEGATNSG QQETQLEEAS QAAAAEGSDQ
60 70 80 90 100
GEQKLKASNG DTPTHEDLTK NKERTSESRG LSRLLSSFLK RPKSQVSEEE
110 120 130 140 150
GREVESEKEK GEGGQKEIEL GNSLDEDIIL KAPIAAPEPE LKTDPSLDLH
160 170 180 190 200
SLSSIETQPA QEEHREDPDS ETKEGEGIEE CSGTEVKEDP ESRAEREPEA
210 220 230 240 250
SQKPVRRHRN MHCKVSLLDD TVYECVVEKH AKGQDLLKRV CEHLNLLEED
260 270 280 290 300
YFGLALWDSA TSKTWLDSAK EIKKQVRGVP WNFTFNVKFY PPDPAQLTED
310 320 330 340 350
ITRYYLCLQL RQDIVAGRLP CSFATLALLG SYTIQSELGD YDPELHGMDY
360 370 380 390 400
VSDFKLAPNQ TKELEEKVME LHKSYRSMTP AQADLEFLEN AKKLSMYGVD
410 420 430 440 450
LHKAKDLEGV DIILGVCSSG LLVYKDKLRI NRFPWPKVLK ISYKRSSFFI
460 470 480 490 500
KIRPGEQEHY ESTIGFKLPS YRAAKKLWKV CVEHHTFFRL TSTDTIPKSK
510 520 530 540 550
FLALGSKFRY SGRTQAQTRQ ASALIDRPAP HFERTASKRA SRSLDGAAAA
560 570 580 590 600
ESTDRSPRPT SAPAIAQSQV TEGPGAPIKK TPKEAVKVEE KRGEEPAEPA
610 620 630 640 650
EPEPTEAWKV EKTHTEVTVP TSNGDQTQKL AGKGEDLIRM RKKKRERLDG
660 670 680 690 700
ENIYIRHSNL MLEDLDKSQE EIKKHHASIS ELKKNFMESV PEPRPSEWDK
710 720 730 740 750
RLSTHSPFRT LNINGQVPTG DGPPLVKTQT VTISDTANAV KSEIPTKDVP
760 770 780 790 800
IVHTETKTIT YEAAQTEDSN GDLDPGVLLT AQTITSETTS STTTTQITKT
810 820 830 840 850
VKGGISETRI EKRIVITGDA DIDHDQVLVQ AIKEAKEQHP DMSVTKVVVH

QETEISEE
Length:858
Mass (Da):95,911
Last modified:May 29, 2007 - v2
Checksum:i7CF1CD52D790D1FD
GO
Isoform 2 (identifier: P48193-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     610-663: Missing.

Show »
Length:804
Mass (Da):89,664
Checksum:iB4BCAD3F40CB7864
GO

Sequence cautioni

The sequence BAD90280.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 33MTT → EPLKGREPRRARTRPGPARP GPCQVPVLCSP in AAH68138. (PubMed:15489334)Curated
Sequence conflicti8 – 81A → V in AAA37123. (PubMed:8429050)Curated
Sequence conflicti232 – 2332KG → NL in AAA37122. (PubMed:8429050)Curated
Sequence conflicti443 – 4431Y → S in AAA37122. (PubMed:8429050)Curated
Sequence conflicti576 – 5761A → R in AAA37122. (PubMed:8429050)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei610 – 66354Missing in isoform 2. 2 PublicationsVSP_012874Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00919 mRNA. Translation: AAA37122.1.
L00919 mRNA. Translation: AAA37123.1.
AK220462 mRNA. Translation: BAD90280.1. Different initiation.
AL607088, AL669981 Genomic DNA. Translation: CAM14971.1.
AL669981, AL607088 Genomic DNA. Translation: CAM21329.1.
BC068138 mRNA. Translation: AAH68138.1.
BC079875 mRNA. Translation: AAH79875.1.
CCDSiCCDS18717.1. [P48193-1]
CCDS51317.1. [P48193-2]
PIRiA46613.
RefSeqiNP_001122078.1. NM_001128606.1. [P48193-2]
NP_001122079.1. NM_001128607.1.
NP_906273.3. NM_183428.3. [P48193-1]
UniGeneiMm.30038.
Mm.471627.

Genome annotation databases

EnsembliENSMUST00000030739; ENSMUSP00000030739; ENSMUSG00000028906. [P48193-1]
ENSMUST00000054917; ENSMUSP00000060375; ENSMUSG00000028906. [P48193-2]
ENSMUST00000084253; ENSMUSP00000081274; ENSMUSG00000028906. [P48193-2]
ENSMUST00000105972; ENSMUSP00000101592; ENSMUSG00000028906. [P48193-1]
ENSMUST00000105981; ENSMUSP00000101601; ENSMUSG00000028906. [P48193-1]
GeneIDi269587.
KEGGimmu:269587.
UCSCiuc008vam.3. mouse. [P48193-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00919 mRNA. Translation: AAA37122.1.
L00919 mRNA. Translation: AAA37123.1.
AK220462 mRNA. Translation: BAD90280.1. Different initiation.
AL607088, AL669981 Genomic DNA. Translation: CAM14971.1.
AL669981, AL607088 Genomic DNA. Translation: CAM21329.1.
BC068138 mRNA. Translation: AAH68138.1.
BC079875 mRNA. Translation: AAH79875.1.
CCDSiCCDS18717.1. [P48193-1]
CCDS51317.1. [P48193-2]
PIRiA46613.
RefSeqiNP_001122078.1. NM_001128606.1. [P48193-2]
NP_001122079.1. NM_001128607.1.
NP_906273.3. NM_183428.3. [P48193-1]
UniGeneiMm.30038.
Mm.471627.

3D structure databases

ProteinModelPortaliP48193.
SMRiP48193. Positions 209-520.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP48193. 3 interactions.
MINTiMINT-149787.

PTM databases

PhosphoSiteiP48193.

Proteomic databases

MaxQBiP48193.
PaxDbiP48193.
PRIDEiP48193.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030739; ENSMUSP00000030739; ENSMUSG00000028906. [P48193-1]
ENSMUST00000054917; ENSMUSP00000060375; ENSMUSG00000028906. [P48193-2]
ENSMUST00000084253; ENSMUSP00000081274; ENSMUSG00000028906. [P48193-2]
ENSMUST00000105972; ENSMUSP00000101592; ENSMUSG00000028906. [P48193-1]
ENSMUST00000105981; ENSMUSP00000101601; ENSMUSG00000028906. [P48193-1]
GeneIDi269587.
KEGGimmu:269587.
UCSCiuc008vam.3. mouse. [P48193-1]

Organism-specific databases

CTDi269587.
MGIiMGI:95401. Epb4.1.
RougeiSearch...

Phylogenomic databases

eggNOGiNOG242913.
GeneTreeiENSGT00760000118823.
HOGENOMiHOG000228841.
HOVERGENiHBG007777.
InParanoidiP48193.
KOiK06107.

Miscellaneous databases

NextBioi392903.
PROiP48193.
SOURCEiSearch...

Gene expression databases

BgeeiP48193.
CleanExiMM_EPB4.1.
ExpressionAtlasiP48193. baseline and differential.
GenevestigatoriP48193.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR008379. Band_4.1_C.
IPR019749. Band_41_domain.
IPR021187. Band_41_protein.
IPR000798. Ez/rad/moesin_like.
IPR014847. FERM-adjacent.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR011993. PH_like_dom.
IPR007477. SAB_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF05902. 4_1_CTD. 1 hit.
PF08736. FA. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF04382. SAB. 1 hit.
[Graphical view]
PIRSFiPIRSF002304. Membrane_skeletal_4_1. 1 hit.
PRINTSiPR00935. BAND41.
PR00661. ERMFAMILY.
SMARTiSM00295. B41. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00660. FERM_1. 1 hit.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic structure of the locus encoding protein 4.1. Structural basis for complex combinational patterns of tissue-specific alternative RNA splicing."
    Huang J.-P., Tang C.-J.C., Kou G.-H., Marchesi V.T., Benz E.J. Jr., Tang T.K.
    J. Biol. Chem. 268:3758-3766(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING.
    Strain: BALB/c.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Fetal brain.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6.
    Tissue: Brain.
  5. "Properties of the C-terminal domain of 4.1 proteins."
    Scott C., Phillips G.W., Baines A.J.
    Eur. J. Biochem. 268:3709-3717(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 709-713, IDENTIFICATION BY MASS SPECTROMETRY, CHARACTERIZATION OF CARBOXY-TERMINAL DOMAIN.
  6. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
    Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
    J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Mast cell.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-541 AND SER-543, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-543, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry namei41_MOUSE
AccessioniPrimary (citable) accession number: P48193
Secondary accession number(s): A2A843
, Q5DTQ8, Q68FF1, Q6NVF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: May 29, 2007
Last modified: February 4, 2015
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.