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Protein

Protein 4.1

Gene

Epb41

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: MGI
  • actomyosin structure organization Source: GO_Central
  • cortical actin cytoskeleton organization Source: InterPro
  • positive regulation of protein binding Source: MGI
  • regulation of cell shape Source: MGI
Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-MMU-6794361. Interactions of neurexins and neuroligins at synapses.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein 4.1
Short name:
P4.1
Alternative name(s):
4.1R
Band 4.1
Gene namesi
Name:Epb41
Synonyms:Epb4.1, Kiaa4056
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:95401. Epb41.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • cell junction Source: MGI
  • cortical cytoskeleton Source: MGI
  • cytoplasm Source: MGI
  • extrinsic component of membrane Source: InterPro
  • membrane Source: MGI
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: MGI
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002193911 – 858Protein 4.1Add BLAST858

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineBy similarity1
Modified residuei62PhosphothreonineBy similarity1
Modified residuei86PhosphoserineBy similarity1
Modified residuei87PhosphoserineBy similarity1
Modified residuei97PhosphoserineBy similarity1
Modified residuei106PhosphoserineBy similarity1
Modified residuei123PhosphoserineCombined sources1
Modified residuei151PhosphoserineBy similarity1
Modified residuei153PhosphoserineBy similarity1
Modified residuei154PhosphoserineBy similarity1
Modified residuei192PhosphoserineBy similarity1
Modified residuei223PhosphotyrosineCombined sources1
Modified residuei379PhosphothreonineBy similarity1
Modified residuei522PhosphoserineBy similarity1
Modified residuei541PhosphoserineCombined sources1
Modified residuei543PhosphoserineCombined sources1
Modified residuei556PhosphoserineCombined sources1
Modified residuei654PhosphotyrosineBy similarity1
Modified residuei658PhosphoserineCombined sources1
Modified residuei668PhosphoserineCombined sources1
Modified residuei678PhosphoserineBy similarity1
Modified residuei703PhosphoserineBy similarity1
Modified residuei706PhosphoserineBy similarity1
Modified residuei730PhosphothreonineBy similarity1
Modified residuei853PhosphothreonineBy similarity1

Post-translational modificationi

O-glycosylated; contains N-acetylglucosamine side chains in the C-terminal domain.By similarity
Phosphorylated at multiple sites by different protein kinases and each phosphorylation event selectively modulates the protein's functions.
Phosphorylation on Tyr-654 reduces the ability of 4.1 to promote the assembly of the spectrin/actin/4.1 ternary complex.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP48193.
PaxDbiP48193.
PeptideAtlasiP48193.
PRIDEiP48193.

PTM databases

iPTMnetiP48193.
PhosphoSitePlusiP48193.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028906.
CleanExiMM_EPB4.1.
ExpressionAtlasiP48193. baseline and differential.
GenevisibleiP48193. MM.

Interactioni

Subunit structurei

Binds with a high affinity to glycophorin and with lower affinity to band III protein. Associates with the nuclear mitotic apparatus. Binds calmodulin and DLG1 (By similarity).By similarity

GO - Molecular functioni

  • actin binding Source: MGI
  • spectrin binding Source: MGI

Protein-protein interaction databases

IntActiP48193. 3 interactors.
MINTiMINT-149787.
STRINGi10090.ENSMUSP00000030739.

Structurei

3D structure databases

ProteinModelPortaliP48193.
SMRiP48193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini211 – 492FERMPROSITE-ProRule annotationAdd BLAST282

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni495 – 608HydrophilicAdd BLAST114
Regioni609 – 707Spectrin--actin-bindingAdd BLAST99
Regioni710 – 858C-terminal (CTD)Add BLAST149

Sequence similaritiesi

Contains 1 FERM domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410KDUP. Eukaryota.
ENOG410XS0M. LUCA.
GeneTreeiENSGT00760000118823.
HOGENOMiHOG000228841.
HOVERGENiHBG007777.
InParanoidiP48193.
KOiK06107.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR008379. Band_4.1_C.
IPR019749. Band_41_domain.
IPR000798. Ez/rad/moesin-like.
IPR014847. FERM-adjacent.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR011993. PH_dom-like.
IPR007477. SAB_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF05902. 4_1_CTD. 1 hit.
PF08736. FA. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF04382. SAB. 1 hit.
[Graphical view]
PRINTSiPR00935. BAND41.
PR00661. ERMFAMILY.
SMARTiSM00295. B41. 1 hit.
SM01195. FA. 1 hit.
SM01196. FERM_C. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00660. FERM_1. 1 hit.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.
Isoform 1 (identifier: P48193-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTEKSLAAE AENSQHQQQK EEGEGATNSG QQETQLEEAS QAAAAEGSDQ
60 70 80 90 100
GEQKLKASNG DTPTHEDLTK NKERTSESRG LSRLLSSFLK RPKSQVSEEE
110 120 130 140 150
GREVESEKEK GEGGQKEIEL GNSLDEDIIL KAPIAAPEPE LKTDPSLDLH
160 170 180 190 200
SLSSIETQPA QEEHREDPDS ETKEGEGIEE CSGTEVKEDP ESRAEREPEA
210 220 230 240 250
SQKPVRRHRN MHCKVSLLDD TVYECVVEKH AKGQDLLKRV CEHLNLLEED
260 270 280 290 300
YFGLALWDSA TSKTWLDSAK EIKKQVRGVP WNFTFNVKFY PPDPAQLTED
310 320 330 340 350
ITRYYLCLQL RQDIVAGRLP CSFATLALLG SYTIQSELGD YDPELHGMDY
360 370 380 390 400
VSDFKLAPNQ TKELEEKVME LHKSYRSMTP AQADLEFLEN AKKLSMYGVD
410 420 430 440 450
LHKAKDLEGV DIILGVCSSG LLVYKDKLRI NRFPWPKVLK ISYKRSSFFI
460 470 480 490 500
KIRPGEQEHY ESTIGFKLPS YRAAKKLWKV CVEHHTFFRL TSTDTIPKSK
510 520 530 540 550
FLALGSKFRY SGRTQAQTRQ ASALIDRPAP HFERTASKRA SRSLDGAAAA
560 570 580 590 600
ESTDRSPRPT SAPAIAQSQV TEGPGAPIKK TPKEAVKVEE KRGEEPAEPA
610 620 630 640 650
EPEPTEAWKV EKTHTEVTVP TSNGDQTQKL AGKGEDLIRM RKKKRERLDG
660 670 680 690 700
ENIYIRHSNL MLEDLDKSQE EIKKHHASIS ELKKNFMESV PEPRPSEWDK
710 720 730 740 750
RLSTHSPFRT LNINGQVPTG DGPPLVKTQT VTISDTANAV KSEIPTKDVP
760 770 780 790 800
IVHTETKTIT YEAAQTEDSN GDLDPGVLLT AQTITSETTS STTTTQITKT
810 820 830 840 850
VKGGISETRI EKRIVITGDA DIDHDQVLVQ AIKEAKEQHP DMSVTKVVVH

QETEISEE
Length:858
Mass (Da):95,911
Last modified:May 29, 2007 - v2
Checksum:i7CF1CD52D790D1FD
GO
Isoform 2 (identifier: P48193-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     610-663: Missing.

Show »
Length:804
Mass (Da):89,664
Checksum:iB4BCAD3F40CB7864
GO

Sequence cautioni

The sequence BAD90280 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 3MTT → EPLKGREPRRARTRPGPARP GPCQVPVLCSP in AAH68138 (PubMed:15489334).Curated3
Sequence conflicti8A → V in AAA37123 (PubMed:8429050).Curated1
Sequence conflicti232 – 233KG → NL in AAA37122 (PubMed:8429050).Curated2
Sequence conflicti443Y → S in AAA37122 (PubMed:8429050).Curated1
Sequence conflicti576A → R in AAA37122 (PubMed:8429050).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012874610 – 663Missing in isoform 2. 2 PublicationsAdd BLAST54

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00919 mRNA. Translation: AAA37122.1.
L00919 mRNA. Translation: AAA37123.1.
AK220462 mRNA. Translation: BAD90280.1. Different initiation.
AL607088, AL669981 Genomic DNA. Translation: CAM14971.1.
AL669981, AL607088 Genomic DNA. Translation: CAM21329.1.
BC068138 mRNA. Translation: AAH68138.1.
BC079875 mRNA. Translation: AAH79875.1.
CCDSiCCDS18717.1. [P48193-1]
CCDS51317.1. [P48193-2]
PIRiA46613.
RefSeqiNP_001122078.1. NM_001128606.1. [P48193-2]
NP_001122079.1. NM_001128607.1.
NP_906273.3. NM_183428.3. [P48193-1]
UniGeneiMm.30038.
Mm.471627.

Genome annotation databases

EnsembliENSMUST00000030739; ENSMUSP00000030739; ENSMUSG00000028906. [P48193-1]
ENSMUST00000054917; ENSMUSP00000060375; ENSMUSG00000028906. [P48193-2]
ENSMUST00000084253; ENSMUSP00000081274; ENSMUSG00000028906. [P48193-2]
ENSMUST00000105972; ENSMUSP00000101592; ENSMUSG00000028906. [P48193-1]
ENSMUST00000105981; ENSMUSP00000101601; ENSMUSG00000028906. [P48193-1]
GeneIDi269587.
KEGGimmu:269587.
UCSCiuc008val.3. mouse. [P48193-2]
uc008vam.3. mouse. [P48193-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00919 mRNA. Translation: AAA37122.1.
L00919 mRNA. Translation: AAA37123.1.
AK220462 mRNA. Translation: BAD90280.1. Different initiation.
AL607088, AL669981 Genomic DNA. Translation: CAM14971.1.
AL669981, AL607088 Genomic DNA. Translation: CAM21329.1.
BC068138 mRNA. Translation: AAH68138.1.
BC079875 mRNA. Translation: AAH79875.1.
CCDSiCCDS18717.1. [P48193-1]
CCDS51317.1. [P48193-2]
PIRiA46613.
RefSeqiNP_001122078.1. NM_001128606.1. [P48193-2]
NP_001122079.1. NM_001128607.1.
NP_906273.3. NM_183428.3. [P48193-1]
UniGeneiMm.30038.
Mm.471627.

3D structure databases

ProteinModelPortaliP48193.
SMRiP48193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP48193. 3 interactors.
MINTiMINT-149787.
STRINGi10090.ENSMUSP00000030739.

PTM databases

iPTMnetiP48193.
PhosphoSitePlusiP48193.

Proteomic databases

MaxQBiP48193.
PaxDbiP48193.
PeptideAtlasiP48193.
PRIDEiP48193.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030739; ENSMUSP00000030739; ENSMUSG00000028906. [P48193-1]
ENSMUST00000054917; ENSMUSP00000060375; ENSMUSG00000028906. [P48193-2]
ENSMUST00000084253; ENSMUSP00000081274; ENSMUSG00000028906. [P48193-2]
ENSMUST00000105972; ENSMUSP00000101592; ENSMUSG00000028906. [P48193-1]
ENSMUST00000105981; ENSMUSP00000101601; ENSMUSG00000028906. [P48193-1]
GeneIDi269587.
KEGGimmu:269587.
UCSCiuc008val.3. mouse. [P48193-2]
uc008vam.3. mouse. [P48193-1]

Organism-specific databases

CTDi2035.
MGIiMGI:95401. Epb41.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410KDUP. Eukaryota.
ENOG410XS0M. LUCA.
GeneTreeiENSGT00760000118823.
HOGENOMiHOG000228841.
HOVERGENiHBG007777.
InParanoidiP48193.
KOiK06107.

Enzyme and pathway databases

ReactomeiR-MMU-6794361. Interactions of neurexins and neuroligins at synapses.

Miscellaneous databases

PROiP48193.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028906.
CleanExiMM_EPB4.1.
ExpressionAtlasiP48193. baseline and differential.
GenevisibleiP48193. MM.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR008379. Band_4.1_C.
IPR019749. Band_41_domain.
IPR000798. Ez/rad/moesin-like.
IPR014847. FERM-adjacent.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR011993. PH_dom-like.
IPR007477. SAB_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF05902. 4_1_CTD. 1 hit.
PF08736. FA. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF04382. SAB. 1 hit.
[Graphical view]
PRINTSiPR00935. BAND41.
PR00661. ERMFAMILY.
SMARTiSM00295. B41. 1 hit.
SM01195. FA. 1 hit.
SM01196. FERM_C. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00660. FERM_1. 1 hit.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei41_MOUSE
AccessioniPrimary (citable) accession number: P48193
Secondary accession number(s): A2A843
, Q5DTQ8, Q68FF1, Q6NVF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: May 29, 2007
Last modified: November 2, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.