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P48193 (41_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein 4.1

Short name=P4.1
Alternative name(s):
4.1R
Band 4.1
Gene names
Name:Epb41
Synonyms:Epb4.1, Kiaa4056
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length858 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes By similarity.

Subunit structure

Binds with a high affinity to glycophorin and with lower affinity to band III protein. Associates with the nuclear mitotic apparatus. Binds calmodulin and DLG1 By similarity.

Subcellular location

Cytoplasmcytoskeleton By similarity. Cytoplasmcell cortex By similarity. Nucleus By similarity.

Post-translational modification

O-glycosylated; contains N-acetylglucosamine side chains in the C-terminal domain By similarity.

Phosphorylated at multiple sites by different protein kinases and each phosphorylation event selectively modulates the protein's functions.

Phosphorylation on Tyr-654 reduces the ability of 4.1 to promote the assembly of the spectrin/actin/4.1 ternary complex.

Sequence similarities

Contains 1 FERM domain.

Sequence caution

The sequence BAD90280.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
Nucleus
   Coding sequence diversityAlternative splicing
   LigandActin-binding
Calmodulin-binding
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processactin cytoskeleton organization

Inferred from sequence or structural similarity PubMed 11274145. Source: MGI

cortical actin cytoskeleton organization

Inferred from electronic annotation. Source: InterPro

positive regulation of protein binding

Inferred from genetic interaction PubMed 20585040. Source: MGI

regulation of cell shape

Inferred from sequence or structural similarity PubMed 11274145. Source: MGI

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: Ensembl

actin cytoskeleton

Inferred from sequence or structural similarity PubMed 11274145. Source: MGI

cortical cytoskeleton

Inferred from direct assay PubMed 18723693. Source: MGI

extrinsic component of membrane

Inferred from electronic annotation. Source: InterPro

membrane

Inferred from direct assay PubMed 18723693. Source: MGI

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from electronic annotation. Source: Ensembl

protein complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_function1-phosphatidylinositol binding

Inferred from electronic annotation. Source: Ensembl

actin binding

Inferred from sequence or structural similarity PubMed 11274145. Source: MGI

spectrin binding

Inferred from sequence orthology PubMed 23704327. Source: MGI

structural molecule activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: A number of isoforms are produced.
Isoform 1 (identifier: P48193-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P48193-2)

The sequence of this isoform differs from the canonical sequence as follows:
     610-663: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 858858Protein 4.1
PRO_0000219391

Regions

Domain211 – 492282FERM
Region495 – 608114Hydrophilic
Region609 – 70799Spectrin--actin-binding
Region710 – 858149C-terminal (CTD)

Amino acid modifications

Modified residue141Phosphoserine By similarity
Modified residue621Phosphothreonine By similarity
Modified residue861Phosphoserine By similarity
Modified residue871Phosphoserine By similarity
Modified residue1511Phosphoserine By similarity
Modified residue1531Phosphoserine By similarity
Modified residue1541Phosphoserine By similarity
Modified residue1921Phosphoserine By similarity
Modified residue2231Phosphotyrosine Ref.6
Modified residue5411Phosphoserine Ref.7
Modified residue5431Phosphoserine Ref.7 Ref.8
Modified residue5561Phosphoserine By similarity
Modified residue6541Phosphotyrosine By similarity
Modified residue7061Phosphoserine By similarity

Natural variations

Alternative sequence610 – 66354Missing in isoform 2.
VSP_012874

Experimental info

Sequence conflict1 – 33MTT → EPLKGREPRRARTRPGPARP GPCQVPVLCSP in AAH68138. Ref.4
Sequence conflict81A → V in AAA37123. Ref.1
Sequence conflict232 – 2332KG → NL in AAA37122. Ref.1
Sequence conflict4431Y → S in AAA37122. Ref.1
Sequence conflict5761A → R in AAA37122. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 29, 2007. Version 2.
Checksum: 7CF1CD52D790D1FD

FASTA85895,911
        10         20         30         40         50         60 
MTTEKSLAAE AENSQHQQQK EEGEGATNSG QQETQLEEAS QAAAAEGSDQ GEQKLKASNG 

        70         80         90        100        110        120 
DTPTHEDLTK NKERTSESRG LSRLLSSFLK RPKSQVSEEE GREVESEKEK GEGGQKEIEL 

       130        140        150        160        170        180 
GNSLDEDIIL KAPIAAPEPE LKTDPSLDLH SLSSIETQPA QEEHREDPDS ETKEGEGIEE 

       190        200        210        220        230        240 
CSGTEVKEDP ESRAEREPEA SQKPVRRHRN MHCKVSLLDD TVYECVVEKH AKGQDLLKRV 

       250        260        270        280        290        300 
CEHLNLLEED YFGLALWDSA TSKTWLDSAK EIKKQVRGVP WNFTFNVKFY PPDPAQLTED 

       310        320        330        340        350        360 
ITRYYLCLQL RQDIVAGRLP CSFATLALLG SYTIQSELGD YDPELHGMDY VSDFKLAPNQ 

       370        380        390        400        410        420 
TKELEEKVME LHKSYRSMTP AQADLEFLEN AKKLSMYGVD LHKAKDLEGV DIILGVCSSG 

       430        440        450        460        470        480 
LLVYKDKLRI NRFPWPKVLK ISYKRSSFFI KIRPGEQEHY ESTIGFKLPS YRAAKKLWKV 

       490        500        510        520        530        540 
CVEHHTFFRL TSTDTIPKSK FLALGSKFRY SGRTQAQTRQ ASALIDRPAP HFERTASKRA 

       550        560        570        580        590        600 
SRSLDGAAAA ESTDRSPRPT SAPAIAQSQV TEGPGAPIKK TPKEAVKVEE KRGEEPAEPA 

       610        620        630        640        650        660 
EPEPTEAWKV EKTHTEVTVP TSNGDQTQKL AGKGEDLIRM RKKKRERLDG ENIYIRHSNL 

       670        680        690        700        710        720 
MLEDLDKSQE EIKKHHASIS ELKKNFMESV PEPRPSEWDK RLSTHSPFRT LNINGQVPTG 

       730        740        750        760        770        780 
DGPPLVKTQT VTISDTANAV KSEIPTKDVP IVHTETKTIT YEAAQTEDSN GDLDPGVLLT 

       790        800        810        820        830        840 
AQTITSETTS STTTTQITKT VKGGISETRI EKRIVITGDA DIDHDQVLVQ AIKEAKEQHP 

       850 
DMSVTKVVVH QETEISEE 

« Hide

Isoform 2 [UniParc].

Checksum: B4BCAD3F40CB7864
Show »

FASTA80489,664

References

« Hide 'large scale' references
[1]"Genomic structure of the locus encoding protein 4.1. Structural basis for complex combinational patterns of tissue-specific alternative RNA splicing."
Huang J.-P., Tang C.-J.C., Kou G.-H., Marchesi V.T., Benz E.J. Jr., Tang T.K.
J. Biol. Chem. 268:3758-3766(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING.
Strain: BALB/c.
[2]"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Fetal brain.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6.
Tissue: Brain.
[5]"Properties of the C-terminal domain of 4.1 proteins."
Scott C., Phillips G.W., Baines A.J.
Eur. J. Biochem. 268:3709-3717(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 709-713, IDENTIFICATION BY MASS SPECTROMETRY, CHARACTERIZATION OF CARBOXY-TERMINAL DOMAIN.
[6]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Mast cell.
[7]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-541 AND SER-543, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[8]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-543, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L00919 mRNA. Translation: AAA37122.1.
L00919 mRNA. Translation: AAA37123.1.
AK220462 mRNA. Translation: BAD90280.1. Different initiation.
AL607088, AL669981 Genomic DNA. Translation: CAM14971.1.
AL669981, AL607088 Genomic DNA. Translation: CAM21329.1.
BC068138 mRNA. Translation: AAH68138.1.
BC079875 mRNA. Translation: AAH79875.1.
CCDSCCDS18717.1. [P48193-1]
CCDS51317.1. [P48193-2]
PIRA46613.
RefSeqNP_001122078.1. NM_001128606.1. [P48193-2]
NP_001122079.1. NM_001128607.1.
NP_906273.3. NM_183428.3. [P48193-1]
UniGeneMm.30038.
Mm.471627.

3D structure databases

ProteinModelPortalP48193.
SMRP48193. Positions 209-520.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP48193. 3 interactions.
MINTMINT-149787.

PTM databases

PhosphoSiteP48193.

Proteomic databases

MaxQBP48193.
PaxDbP48193.
PRIDEP48193.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030739; ENSMUSP00000030739; ENSMUSG00000028906. [P48193-1]
ENSMUST00000054917; ENSMUSP00000060375; ENSMUSG00000028906. [P48193-2]
ENSMUST00000084253; ENSMUSP00000081274; ENSMUSG00000028906. [P48193-2]
ENSMUST00000105972; ENSMUSP00000101592; ENSMUSG00000028906. [P48193-1]
ENSMUST00000105981; ENSMUSP00000101601; ENSMUSG00000028906. [P48193-1]
GeneID269587.
KEGGmmu:269587.
UCSCuc008vam.3. mouse. [P48193-1]

Organism-specific databases

CTD269587.
MGIMGI:95401. Epb4.1.
RougeSearch...

Phylogenomic databases

eggNOGNOG242913.
GeneTreeENSGT00750000117241.
HOGENOMHOG000228841.
HOVERGENHBG007777.
KOK06107.

Gene expression databases

ArrayExpressP48193.
BgeeP48193.
CleanExMM_EPB4.1.
GenevestigatorP48193.

Family and domain databases

Gene3D1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
InterProIPR008379. Band_4.1_C.
IPR019749. Band_41_domain.
IPR019750. Band_41_fam.
IPR021187. Band_41_protein.
IPR000798. Ez/rad/moesin_like.
IPR014847. FERM-adjacent.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR011993. PH_like_dom.
IPR007477. SAB_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamPF05902. 4_1_CTD. 1 hit.
PF08736. FA. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF04382. SAB. 1 hit.
[Graphical view]
PIRSFPIRSF002304. Membrane_skeletal_4_1. 1 hit.
PRINTSPR00935. BAND41.
PR00661. ERMFAMILY.
SMARTSM00295. B41. 1 hit.
[Graphical view]
SUPFAMSSF47031. SSF47031. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEPS00660. FERM_1. 1 hit.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio392903.
PROP48193.
SOURCESearch...

Entry information

Entry name41_MOUSE
AccessionPrimary (citable) accession number: P48193
Secondary accession number(s): A2A843 expand/collapse secondary AC list , Q5DTQ8, Q68FF1, Q6NVF5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: May 29, 2007
Last modified: July 9, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot