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P48180 (ACH1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acetylcholine receptor subunit alpha-type acr-16
Gene names
Name:acr-16
Synonyms:Ce21
ORF Names:F25G6.3
OrganismCaenorhabditis elegans
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length498 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane By similarity. A subunit of the levamisole-insensitive nicotinic receptor. Ref.1 Ref.4

Subcellular location

Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note: The cam-1 protein is required for correct localization. Ref.5

Tissue specificity

Expressed in the body wall muscle. Ref.4

Disruption phenotype

Uncoordinated movement and reduced body wall muscle currents. These phenotypes are probably related. Ref.5 Ref.6

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: P48180-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: P48180-2)

The sequence of this isoform differs from the canonical sequence as follows:
     470-480: VDRLCLYVFTI → RTYISRKIENF
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 498479Acetylcholine receptor subunit alpha-type acr-16
PRO_0000000395

Regions

Topological domain20 – 230211Extracellular Potential
Transmembrane231 – 25222Helical; Potential
Transmembrane261 – 27919Helical; Potential
Transmembrane295 – 31420Helical; Potential
Topological domain315 – 472158Cytoplasmic Potential
Transmembrane473 – 49321Helical; Potential

Amino acid modifications

Glycosylation431N-linked (GlcNAc...) Potential
Glycosylation931N-linked (GlcNAc...) Potential
Disulfide bond147 ↔ 161 By similarity
Disulfide bond211 ↔ 212Associated with receptor activation By similarity

Natural variations

Alternative sequence470 – 48011VDRLCLYVFTI → RTYISRKIENF in isoform b.
VSP_036472

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: E463ABB40AC9FA82

FASTA49857,169
        10         20         30         40         50         60 
MSVCTLLISC AILAAPTLGS LQERRLYEDL MRNYNNLERP VANHSEPVTV HLKVALQQII 

        70         80         90        100        110        120 
DVDEKNQVVY VNAWLDYTWN DYNLVWDKAE YGNITDVRFP AGKIWKPDVL LYNSVDTNFD 

       130        140        150        160        170        180 
STYQTNMIVY STGLVHWVPP GIFKISCKID IQWFPFDEQK CFFKFGSWTY DGYKLDLQPA 

       190        200        210        220        230        240 
TGGFDISEYI SNGEWALPLT TVERNEKFYD CCPEPYPDVH FYLHMRRRTL YYGFNLIMPC 

       250        260        270        280        290        300 
ILTTLMTLLG FTLPPDAGEK ITLQITVLLS ICFFLSIVSE MSPPTSEAVP LLGIFFTCCM 

       310        320        330        340        350        360 
IVVTASTVFT VYVLNLHYRT PETHDMGPWT RNLLLYWIPW ILRMKRPGHN LTYASLPSLF 

       370        380        390        400        410        420 
STKPNRHSES LIRNIKDNEH SLSRANSFDA DCRLNQYIMT QSVSNGLTSL GSIPSTMISS 

       430        440        450        460        470        480 
NGTTTDVSQQ ATLLILHRIY HELKIVTKRM IEGDKEEQAC NNWKFAAMVV DRLCLYVFTI 

       490 
FIIVSTIGIF WSAPYLVA

« Hide

Isoform b [UniParc].

Checksum: 8699EB8BBC825C44
Show »

FASTA49857,254

References

« Hide 'large scale' references
[1]"Nicotinic acetylcholine receptors in the nematode Caenorhabditis elegans."
Ballivet M., Alliod C., Bertrand S., Bertrand D.
J. Mol. Biol. 258:261-269(1996) [PubMed: 8627624] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), FUNCTION.
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[3]"The Caenorhabditis elegans transcriptome project, a complementary view of the genome."
Kohara Y., Shin-i T., Suzuki Y., Sugano S., Thierry-Mieg D., Thierry-Mieg J.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
Strain: Bristol N2.
[4]"acr-16 encodes an essential subunit of the levamisole-resistant nicotinic receptor at the Caenorhabditis elegans neuromuscular junction."
Touroutine D., Fox R.M., Von Stetina S.E., Burdina A., Miller D.M. III, Richmond J.E.
J. Biol. Chem. 280:27013-27021(2005) [PubMed: 15917232] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[5]"The Ror receptor tyrosine kinase CAM-1 is required for ACR-16-mediated synaptic transmission at the C. elegans neuromuscular junction."
Francis M.M., Evans S.P., Jensen M., Madsen D.M., Mancuso J., Norman K.R., Maricq A.V.
Neuron 46:581-594(2005) [PubMed: 15944127] [Abstract]
Cited for: SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[6]"PKC-1 regulates secretion of neuropeptides."
Sieburth D., Madison J.M., Kaplan J.M.
Nat. Neurosci. 10:49-57(2007) [PubMed: 17128266] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X83887 mRNA. Translation: CAA58764.1.
FO080489 Genomic DNA. Translation: CCD64102.1.
FO080489 Genomic DNA. Translation: CCD64103.1.
AY523511 mRNA. Translation: AAR98633.1.
PIRS68588.
RefSeqNP_001023847.1. NM_001028676.2.
NP_505207.1. NM_072806.3.
UniGeneCel.18045.

3D structure databases

ProteinModelPortalP48180.
SMRP48180. Positions 20-493.
ModBaseSearch...

Proteomic databases

PRIDEP48180.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaF25G6.3a; F25G6.3a; F25G6.3.
GeneID179235.
KEGGcel:F25G6.3.
UCSCF25G6.3a.1. c. elegans.

Organism-specific databases

CTD179235.
WormBaseF25G6.3a; CE09639; WBGene00000055; acr-16.
F25G6.3b; CE32878; WBGene00000055; acr-16.

Phylogenomic databases

eggNOGmeNOG06474.
GeneTreeEMGT00050000005191.
HOGENOMHBG387619.
InParanoidP48180.
OMAMISSANG.
PhylomeDBP48180.

Gene expression databases

ArrayExpressP48180.

Family and domain databases

InterProIPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
Gene3DG3DSA:2.70.170.10. Neur_chan_lig_bd. 1 hit.
KOK05312.
PANTHERPTHR18945. Neur_channel. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMSSF90112. Neu_channel_TM. 1 hit.
SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMsTIGR00860. LIC. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio904496.

Entry information

Entry nameACH1_CAEEL
AccessionPrimary (citable) accession number: P48180
Secondary accession number(s): Q8I932
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents