Reviewed, UniProtKB/Swiss-Prot P48180 (ACH1_CAEEL)

Names and origin

Protein names

Recommended name:
    Acetylcholine receptor subunit alpha-type acr-16

Gene names

Name:	acr-16
Synonyms:	Ce21
ORF Names:	F25G6.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane By similarity. A subunit of the levamisole-insensitive nicotinic receptor.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note: The cam-1 protein is required for correct localization. Ref.5
Tissue specificity	Expressed in the body wall muscle. Ref.4
Disruption phenotype	Uncoordinated movement and reduced body wall muscle currents. These phenotypes are probably related. Ref.5 Ref.6
Sequence similarities	Belongs to the ligand-gated ionic channel (TC 1.A.9) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane
Molecular function	Ionic channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ion transport Inferred from electronic annotation. Source: UniProtKB-KW locomotion Ref.5 Inferred from mutant phenotype. Source: WormBase
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nicotinic acetylcholine-activated cation-selective channel activity Inferred from electronic annotation. Source: InterPro receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: P48180-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: P48180-2)

The sequence of this isoform differs from the canonical sequence as follows:
     470-480: VDRLCLYVFTI → RTYISRKIENF

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 498	479	Acetylcholine receptor subunit alpha-type acr-16		PRO_0000000395
Regions
	Topological domain	20 – 230	211	Extracellular Potential
	Transmembrane	231 – 252	22	Potential
	Transmembrane	261 – 279	19	Potential
	Transmembrane	295 – 314	20	Potential
	Topological domain	315 – 472	158	Cytoplasmic Potential
	Transmembrane	473 – 493	21	Potential
Amino acid modifications
	Glycosylation	43	1	N-linked (GlcNAc...) Potential
	Glycosylation	93	1	N-linked (GlcNAc...) Potential
	Disulfide bond	147 ↔ 161		By similarity
	Disulfide bond	211 ↔ 212		Associated with receptor activation By similarity
Natural variations
	Alternative sequence	470 – 480	11	VDRLCLYVFTI → RTYISRKIENF in isoform b.		VSP_036472

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified February 1, 1996. Version 1. Checksum: E463ABB40AC9FA82	FASTA	498	57,169
	10 20 30 40 50 60 MSVCTLLISC AILAAPTLGS LQERRLYEDL MRNYNNLERP VANHSEPVTV HLKVALQQII 70 80 90 100 110 120 DVDEKNQVVY VNAWLDYTWN DYNLVWDKAE YGNITDVRFP AGKIWKPDVL LYNSVDTNFD 130 140 150 160 170 180 STYQTNMIVY STGLVHWVPP GIFKISCKID IQWFPFDEQK CFFKFGSWTY DGYKLDLQPA 190 200 210 220 230 240 TGGFDISEYI SNGEWALPLT TVERNEKFYD CCPEPYPDVH FYLHMRRRTL YYGFNLIMPC 250 260 270 280 290 300 ILTTLMTLLG FTLPPDAGEK ITLQITVLLS ICFFLSIVSE MSPPTSEAVP LLGIFFTCCM 310 320 330 340 350 360 IVVTASTVFT VYVLNLHYRT PETHDMGPWT RNLLLYWIPW ILRMKRPGHN LTYASLPSLF 370 380 390 400 410 420 STKPNRHSES LIRNIKDNEH SLSRANSFDA DCRLNQYIMT QSVSNGLTSL GSIPSTMISS 430 440 450 460 470 480 NGTTTDVSQQ ATLLILHRIY HELKIVTKRM IEGDKEEQAC NNWKFAAMVV DRLCLYVFTI 490 FIIVSTIGIF WSAPYLVA
	Isoform b. Checksum: 8699EB8BBC825C44	FASTA	498	57,254
	10 20 30 40 50 60 MSVCTLLISC AILAAPTLGS LQERRLYEDL MRNYNNLERP VANHSEPVTV HLKVALQQII 70 80 90 100 110 120 DVDEKNQVVY VNAWLDYTWN DYNLVWDKAE YGNITDVRFP AGKIWKPDVL LYNSVDTNFD 130 140 150 160 170 180 STYQTNMIVY STGLVHWVPP GIFKISCKID IQWFPFDEQK CFFKFGSWTY DGYKLDLQPA 190 200 210 220 230 240 TGGFDISEYI SNGEWALPLT TVERNEKFYD CCPEPYPDVH FYLHMRRRTL YYGFNLIMPC 250 260 270 280 290 300 ILTTLMTLLG FTLPPDAGEK ITLQITVLLS ICFFLSIVSE MSPPTSEAVP LLGIFFTCCM 310 320 330 340 350 360 IVVTASTVFT VYVLNLHYRT PETHDMGPWT RNLLLYWIPW ILRMKRPGHN LTYASLPSLF 370 380 390 400 410 420 STKPNRHSES LIRNIKDNEH SLSRANSFDA DCRLNQYIMT QSVSNGLTSL GSIPSTMISS 430 440 450 460 470 480 NGTTTDVSQQ ATLLILHRIY HELKIVTKRM IEGDKEEQAC NNWKFAAMVR TYISRKIENF 490 FIIVSTIGIF WSAPYLVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nicotinic acetylcholine receptors in the nematode Caenorhabditis elegans." Ballivet M., Alliod C., Bertrand S., Bertrand D. J. Mol. Biol. 258:261-269(1996) [PubMed: 8627624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), FUNCTION. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[3]	"The Caenorhabditis elegans transcriptome project, a complementary view of the genome." Kohara Y., Shin-i T., Suzuki Y., Sugano S., Thierry-Mieg D., Thierry-Mieg J. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Bristol N2.
[4]	"acr-16 encodes an essential subunit of the levamisole-resistant nicotinic receptor at the Caenorhabditis elegans neuromuscular junction." Touroutine D., Fox R.M., Von Stetina S.E., Burdina A., Miller D.M. III, Richmond J.E. J. Biol. Chem. 280:27013-27021(2005) [PubMed: 15917232] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[5]	"The Ror receptor tyrosine kinase CAM-1 is required for ACR-16-mediated synaptic transmission at the C. elegans neuromuscular junction." Francis M.M., Evans S.P., Jensen M., Madsen D.M., Mancuso J., Norman K.R., Maricq A.V. Neuron 46:581-594(2005) [PubMed: 15944127] [Abstract] Cited for: SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[6]	"PKC-1 regulates secretion of neuropeptides." Sieburth D., Madison J.M., Kaplan J.M. Nat. Neurosci. 10:49-57(2007) [PubMed: 17128266] [Abstract] Cited for: DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X83887 mRNA. Translation: CAA58764.1. AF022973 Genomic DNA. Translation: AAC25796.1. AF022973 Genomic DNA. Translation: AAN84815.1. AY523511 mRNA. Translation: AAR98633.1.
PIR	S68588.
RefSeq	NP_001023847.1. NP_505207.1.
UniGene	Cel.18045
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P48180.
Genome annotation databases
Ensembl	F25G6.3a.1; F25G6.3a.1; F25G6.3; Caenorhabditis elegans. [Genome view] F25G6.3a.2; F25G6.3a.2; F25G6.3; Caenorhabditis elegans. [Genome view]
GeneID	179235.
KEGG	cel:F25G6.3.
UCSC	F25G6.3a.1. c. elegans.
Organism-specific databases
CTD	179235.
WormBase	WBGene00000055. acr-16.
WormPep	F25G6.3a. CE09639. [WorfDB] F25G6.3b. CE32878. [WorfDB]
Phylogenomic databases
HOGENOM	HBG387619.
InParanoid	P48180.
OMA	SNGEWAL.
Gene expression databases
ArrayExpress	P48180.
Family and domain databases
InterPro	IPR006029. Neu_channel_TM. IPR006202. Neur_chan_lig_bd. IPR006201. Neur_channel. IPR018000. Neurotransmitter_ion_chnl_CS. IPR002394. Nicotinic_acetylcholine_rcpt_N. [Graphical view]
Gene3D	G3DSA:2.70.170.10. Neur_chan_lig_bd. 1 hit.
PANTHER	PTHR18945. Neur_channel. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR00254. NICOTINICR. PR00252. NRIONCHANNEL.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	904496.

Entry information

Entry name

ACH1_CAEEL

Accession

Primary (citable) accession number: P48180
Secondary accession number(s): Q8I932

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 15, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project