ID   GBRA4_HUMAN             Reviewed;         554 AA.
AC   P48169; Q8IYR7;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 2.
DT   24-JAN-2024, entry version 204.
DE   RecName: Full=Gamma-aminobutyric acid receptor subunit alpha-4;
DE   AltName: Full=GABA(A) receptor subunit alpha-4;
DE   Flags: Precursor;
GN   Name=GABRA4;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
RC   TISSUE=Brain cortex;
RX   PubMed=8719416; DOI=10.1016/0922-4106(95)90072-1;
RA   Yang W., Drewe J.A., Lan N.C.;
RT   "Cloning and characterization of the human GABAA receptor alpha 4 subunit:
RT   identification of a unique diazepam-insensitive binding site.";
RL   Eur. J. Pharmacol. 291:319-325(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15815621; DOI=10.1038/nature03466;
RA   Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA   Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA   Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA   Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA   Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA   Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA   Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA   Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA   Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA   McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA   Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA   Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA   Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA   Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA   Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA   Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA   Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA   Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA   Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA   Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA   Wilson R.K.;
RT   "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT   4.";
RL   Nature 434:724-731(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-57.
RX   PubMed=7607683; DOI=10.1016/0888-7543(95)80178-o;
RA   McLean P.J., Farb D.H., Russek S.J.;
RT   "Mapping of the alpha 4 subunit gene (GABRA4) to human chromosome 4 defines
RT   an alpha 2-alpha 4-beta 1-gamma 1 gene cluster: further evidence that
RT   modern GABAA receptor gene clusters are derived from an ancestral
RT   cluster.";
RL   Genomics 26:580-586(1995).
RN   [7]
RP   VARIANT [LARGE SCALE ANALYSIS] ARG-516.
RX   PubMed=16959974; DOI=10.1126/science.1133427;
RA   Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA   Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA   Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA   Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA   Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA   Velculescu V.E.;
RT   "The consensus coding sequences of human breast and colorectal cancers.";
RL   Science 314:268-274(2006).
CC   -!- FUNCTION: GABA, the major inhibitory neurotransmitter in the vertebrate
CC       brain, mediates neuronal inhibition by binding to the
CC       GABA/benzodiazepine receptor and opening an integral chloride channel.
CC   -!- SUBUNIT: Generally pentameric. There are five types of GABA(A) receptor
CC       chains: alpha, beta, gamma, delta, and rho.
CC   -!- SUBCELLULAR LOCATION: Postsynaptic cell membrane; Multi-pass membrane
CC       protein. Cell membrane; Multi-pass membrane protein.
CC   -!- INDUCTION: The alpha4 beta2 gamma 2L receptor is not repressed by
CC       diazepam. {ECO:0000269|PubMed:8719416}.
CC   -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family.
CC       Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA4 sub-
CC       subfamily. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=Forbidden fruit - Issue 56
CC       of March 2005;
CC       URL="https://web.expasy.org/spotlight/back_issues/056";
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DR   EMBL; U30461; AAB52519.1; -; mRNA.
DR   EMBL; AK090780; BAG52228.1; -; mRNA.
DR   EMBL; AC107383; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471069; EAW93029.1; -; Genomic_DNA.
DR   EMBL; BC035055; AAH35055.1; -; mRNA.
DR   EMBL; U20166; AAA74743.1; -; Genomic_DNA.
DR   CCDS; CCDS3473.1; -.
DR   PIR; G01928; G01928.
DR   RefSeq; NP_000800.2; NM_000809.3.
DR   RefSeq; NP_001191195.1; NM_001204266.1.
DR   RefSeq; NP_001191196.1; NM_001204267.1.
DR   PDB; 7QN5; EM; 2.50 A; A=1-554.
DR   PDB; 7QN7; EM; 3.00 A; A=1-554.
DR   PDB; 7QN9; EM; 2.90 A; A=1-554.
DR   PDB; 7QNA; EM; 3.00 A; A=1-554.
DR   PDB; 7QNC; EM; 2.90 A; A=1-554.
DR   PDBsum; 7QN5; -.
DR   PDBsum; 7QN7; -.
DR   PDBsum; 7QN9; -.
DR   PDBsum; 7QNA; -.
DR   PDBsum; 7QNC; -.
DR   AlphaFoldDB; P48169; -.
DR   EMDB; EMD-14067; -.
DR   EMDB; EMD-14069; -.
DR   EMDB; EMD-14071; -.
DR   EMDB; EMD-14072; -.
DR   EMDB; EMD-14074; -.
DR   SMR; P48169; -.
DR   BioGRID; 108831; 11.
DR   ComplexPortal; CPX-2953; GABA-A receptor, alpha-4/beta-2/delta.
DR   ComplexPortal; CPX-2954; GABA-A receptor, alpha-4/beta-3/delta.
DR   IntAct; P48169; 1.
DR   STRING; 9606.ENSP00000264318; -.
DR   BindingDB; P48169; -.
DR   ChEMBL; CHEMBL2472; -.
DR   DrugBank; DB12537; 1,2-Benzodiazepine.
DR   DrugBank; DB00546; Adinazolam.
DR   DrugBank; DB00404; Alprazolam.
DR   DrugBank; DB01351; Amobarbital.
DR   DrugBank; DB00543; Amoxapine.
DR   DrugBank; DB11901; Apalutamide.
DR   DrugBank; DB01352; Aprobarbital.
DR   DrugBank; DB01483; Barbital.
DR   DrugBank; DB14719; Bentazepam.
DR   DrugBank; DB11859; Brexanolone.
DR   DrugBank; DB01558; Bromazepam.
DR   DrugBank; DB09017; Brotizolam.
DR   DrugBank; DB00237; Butabarbital.
DR   DrugBank; DB00241; Butalbital.
DR   DrugBank; DB01353; Butobarbital.
DR   DrugBank; DB01489; Camazepam.
DR   DrugBank; DB00475; Chlordiazepoxide.
DR   DrugBank; DB14715; Cinazepam.
DR   DrugBank; DB01594; Cinolazepam.
DR   DrugBank; DB00349; Clobazam.
DR   DrugBank; DB01068; Clonazepam.
DR   DrugBank; DB00628; Clorazepic acid.
DR   DrugBank; DB01559; Clotiazepam.
DR   DrugBank; DB01553; Cloxazolam.
DR   DrugBank; DB01511; Delorazepam.
DR   DrugBank; DB01189; Desflurane.
DR   DrugBank; DB00829; Diazepam.
DR   DrugBank; DB01496; Dihydro-2-thioxo-5-((5-(2-(trifluoromethyl)phenyl)-2-furanyl)methyl)-4,6(1H,5H)-pyrimidinedione.
DR   DrugBank; DB13837; Doxefazepam.
DR   DrugBank; DB00228; Enflurane.
DR   DrugBank; DB01215; Estazolam.
DR   DrugBank; DB00402; Eszopiclone.
DR   DrugBank; DB00898; Ethanol.
DR   DrugBank; DB00189; Ethchlorvynol.
DR   DrugBank; DB01545; Ethyl loflazepate.
DR   DrugBank; DB09166; Etizolam.
DR   DrugBank; DB00292; Etomidate.
DR   DrugBank; DB01205; Flumazenil.
DR   DrugBank; DB01544; Flunitrazepam.
DR   DrugBank; DB00690; Flurazepam.
DR   DrugBank; DB05087; Ganaxolone.
DR   DrugBank; DB01437; Glutethimide.
DR   DrugBank; DB00801; Halazepam.
DR   DrugBank; DB01159; Halothane.
DR   DrugBank; DB01354; Heptabarbital.
DR   DrugBank; DB01355; Hexobarbital.
DR   DrugBank; DB00753; Isoflurane.
DR   DrugBank; DB01587; Ketazolam.
DR   DrugBank; DB00555; Lamotrigine.
DR   DrugBank; DB13643; Loprazolam.
DR   DrugBank; DB00186; Lorazepam.
DR   DrugBank; DB13872; Lormetazepam.
DR   DrugBank; DB13437; Medazepam.
DR   DrugBank; DB00603; Medroxyprogesterone acetate.
DR   DrugBank; DB01043; Memantine.
DR   DrugBank; DB00371; Meprobamate.
DR   DrugBank; DB00463; Metharbital.
DR   DrugBank; DB01028; Methoxyflurane.
DR   DrugBank; DB00849; Methylphenobarbital.
DR   DrugBank; DB01107; Methyprylon.
DR   DrugBank; DB15489; Mexazolam.
DR   DrugBank; DB00683; Midazolam.
DR   DrugBank; DB01595; Nitrazepam.
DR   DrugBank; DB14028; Nordazepam.
DR   DrugBank; DB00842; Oxazepam.
DR   DrugBank; DB14672; Oxazepam acetate.
DR   DrugBank; DB00312; Pentobarbital.
DR   DrugBank; DB00252; Phenytoin.
DR   DrugBank; DB13335; Pinazepam.
DR   DrugBank; DB01708; Prasterone.
DR   DrugBank; DB01588; Prazepam.
DR   DrugBank; DB00794; Primidone.
DR   DrugBank; DB00818; Propofol.
DR   DrugBank; DB01589; Quazepam.
DR   DrugBank; DB12404; Remimazolam.
DR   DrugBank; DB00418; Secobarbital.
DR   DrugBank; DB01236; Sevoflurane.
DR   DrugBank; DB09118; Stiripentol.
DR   DrugBank; DB00306; Talbutal.
DR   DrugBank; DB01956; Taurine.
DR   DrugBank; DB00231; Temazepam.
DR   DrugBank; DB11582; Thiocolchicoside.
DR   DrugBank; DB00599; Thiopental.
DR   DrugBank; DB00897; Triazolam.
DR   DrugCentral; P48169; -.
DR   GuidetoPHARMACOLOGY; 407; -.
DR   GlyCosmos; P48169; 3 sites, No reported glycans.
DR   GlyGen; P48169; 3 sites.
DR   iPTMnet; P48169; -.
DR   PhosphoSitePlus; P48169; -.
DR   BioMuta; GABRA4; -.
DR   DMDM; 206729865; -.
DR   MassIVE; P48169; -.
DR   PaxDb; 9606-ENSP00000264318; -.
DR   PeptideAtlas; P48169; -.
DR   ProteomicsDB; 55870; -.
DR   TopDownProteomics; P48169; -.
DR   Antibodypedia; 12041; 467 antibodies from 39 providers.
DR   DNASU; 2557; -.
DR   Ensembl; ENST00000264318.4; ENSP00000264318.3; ENSG00000109158.11.
DR   GeneID; 2557; -.
DR   KEGG; hsa:2557; -.
DR   MANE-Select; ENST00000264318.4; ENSP00000264318.3; NM_000809.4; NP_000800.2.
DR   AGR; HGNC:4078; -.
DR   CTD; 2557; -.
DR   DisGeNET; 2557; -.
DR   GeneCards; GABRA4; -.
DR   HGNC; HGNC:4078; GABRA4.
DR   HPA; ENSG00000109158; Group enriched (brain, heart muscle).
DR   MIM; 137141; gene.
DR   neXtProt; NX_P48169; -.
DR   OpenTargets; ENSG00000109158; -.
DR   PharmGKB; PA28492; -.
DR   VEuPathDB; HostDB:ENSG00000109158; -.
DR   eggNOG; KOG3642; Eukaryota.
DR   GeneTree; ENSGT00940000159024; -.
DR   HOGENOM; CLU_010920_2_2_1; -.
DR   InParanoid; P48169; -.
DR   OMA; FFCLTTC; -.
DR   OrthoDB; 4265336at2759; -.
DR   PhylomeDB; P48169; -.
DR   TreeFam; TF315453; -.
DR   PathwayCommons; P48169; -.
DR   Reactome; R-HSA-977443; GABA receptor activation.
DR   SignaLink; P48169; -.
DR   SIGNOR; P48169; -.
DR   BioGRID-ORCS; 2557; 7 hits in 1155 CRISPR screens.
DR   ChiTaRS; GABRA4; human.
DR   GeneWiki; GABRA4; -.
DR   GenomeRNAi; 2557; -.
DR   Pharos; P48169; Tclin.
DR   PRO; PR:P48169; -.
DR   Proteomes; UP000005640; Chromosome 4.
DR   RNAct; P48169; Protein.
DR   Bgee; ENSG00000109158; Expressed in postcentral gyrus and 92 other cell types or tissues.
DR   ExpressionAtlas; P48169; baseline and differential.
DR   GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR   GO; GO:0032590; C:dendrite membrane; IBA:GO_Central.
DR   GO; GO:1902711; C:GABA-A receptor complex; IPI:ComplexPortal.
DR   GO; GO:0098982; C:GABA-ergic synapse; IEA:Ensembl.
DR   GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR   GO; GO:0098794; C:postsynapse; IBA:GO_Central.
DR   GO; GO:0099634; C:postsynaptic specialization membrane; IEA:Ensembl.
DR   GO; GO:0045202; C:synapse; IBA:GO_Central.
DR   GO; GO:0008503; F:benzodiazepine receptor activity; TAS:ProtInc.
DR   GO; GO:0004890; F:GABA-A receptor activity; IEA:InterPro.
DR   GO; GO:0022851; F:GABA-gated chloride ion channel activity; IBA:GO_Central.
DR   GO; GO:0005237; F:inhibitory extracellular ligand-gated monoatomic ion channel activity; IBA:GO_Central.
DR   GO; GO:1904315; F:transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential; IEA:Ensembl.
DR   GO; GO:1902476; P:chloride transmembrane transport; IDA:ComplexPortal.
DR   GO; GO:0007214; P:gamma-aminobutyric acid signaling pathway; IDA:ComplexPortal.
DR   GO; GO:0060078; P:regulation of postsynaptic membrane potential; IBA:GO_Central.
DR   GO; GO:0051932; P:synaptic transmission, GABAergic; IBA:GO_Central.
DR   CDD; cd19037; LGIC_ECD_GABAAR_A4; 1.
DR   CDD; cd19052; LGIC_TM_GABAAR_alpha; 1.
DR   Gene3D; 2.70.170.10; Neurotransmitter-gated ion-channel ligand-binding domain; 1.
DR   Gene3D; 1.20.58.390; Neurotransmitter-gated ion-channel transmembrane domain; 1.
DR   InterPro; IPR006028; GABAA/Glycine_rcpt.
DR   InterPro; IPR001390; GABAAa_rcpt.
DR   InterPro; IPR005434; GABBAa4_rcpt.
DR   InterPro; IPR047024; Gabra-1-6_TM.
DR   InterPro; IPR006202; Neur_chan_lig-bd.
DR   InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR   InterPro; IPR006201; Neur_channel.
DR   InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR   InterPro; IPR038050; Neuro_actylchol_rec.
DR   InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR   InterPro; IPR018000; Neurotransmitter_ion_chnl_CS.
DR   NCBIfam; TIGR00860; LIC; 1.
DR   PANTHER; PTHR18945:SF393; GAMMA-AMINOBUTYRIC ACID RECEPTOR SUBUNIT ALPHA-4; 1.
DR   PANTHER; PTHR18945; NEUROTRANSMITTER GATED ION CHANNEL; 1.
DR   Pfam; PF02931; Neur_chan_LBD; 1.
DR   Pfam; PF02932; Neur_chan_memb; 1.
DR   PRINTS; PR01079; GABAARALPHA.
DR   PRINTS; PR01617; GABAARALPHA4.
DR   PRINTS; PR00253; GABAARECEPTR.
DR   PRINTS; PR00252; NRIONCHANNEL.
DR   SUPFAM; SSF90112; Neurotransmitter-gated ion-channel transmembrane pore; 1.
DR   SUPFAM; SSF63712; Nicotinic receptor ligand binding domain-like; 1.
DR   PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
DR   Genevisible; P48169; HS.
PE   1: Evidence at protein level;
KW   3D-structure; Cell membrane; Chloride; Chloride channel; Disulfide bond;
KW   Glycoprotein; Ion channel; Ion transport; Ligand-gated ion channel;
KW   Membrane; Postsynaptic cell membrane; Receptor; Reference proteome; Signal;
KW   Synapse; Transmembrane; Transmembrane helix; Transport.
FT   SIGNAL          1..35
FT                   /evidence="ECO:0000255"
FT   CHAIN           36..554
FT                   /note="Gamma-aminobutyric acid receptor subunit alpha-4"
FT                   /id="PRO_0000000441"
FT   TOPO_DOM        36..258
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        259..280
FT                   /note="Helical"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        285..306
FT                   /note="Helical"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        318..340
FT                   /note="Helical"
FT                   /evidence="ECO:0000305"
FT   TOPO_DOM        341..523
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        524..543
FT                   /note="Helical"
FT                   /evidence="ECO:0000305"
FT   REGION          350..381
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          397..435
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          452..471
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          495..515
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        47
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        144
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        157
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        172..186
FT                   /evidence="ECO:0000250"
FT   VARIANT         19
FT                   /note="A -> T (in dbSNP:rs16859837)"
FT                   /id="VAR_046552"
FT   VARIANT         26
FT                   /note="L -> M (in dbSNP:rs2229940)"
FT                   /id="VAR_046553"
FT   VARIANT         516
FT                   /note="S -> R (in a breast cancer sample; somatic
FT                   mutation)"
FT                   /evidence="ECO:0000269|PubMed:16959974"
FT                   /id="VAR_036032"
FT   CONFLICT        316
FT                   /note="A -> L (in Ref. 1; AAB52519)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        379
FT                   /note="N -> D (in Ref. 1; AAB52519)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        463
FT                   /note="Y -> H (in Ref. 1; AAB52519)"
FT                   /evidence="ECO:0000305"
FT   HELIX           49..56
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   TURN            65..68
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   STRAND          72..87
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   TURN            88..91
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   STRAND          92..104
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   HELIX           106..108
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   STRAND          113..119
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   HELIX           121..126
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   STRAND          132..134
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   STRAND          137..142
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   STRAND          145..147
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   STRAND          150..155
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   STRAND          158..171
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   HELIX           176..178
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   STRAND          183..194
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   TURN            197..199
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   STRAND          200..206
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   TURN            208..210
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   STRAND          211..213
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   HELIX           216..218
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   STRAND          222..237
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   STRAND          242..254
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   HELIX           257..262
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   HELIX           264..276
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   HELIX           277..279
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   HELIX           285..309
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   HELIX           318..348
FT                   /evidence="ECO:0007829|PDB:7QN5"
FT   HELIX           517..543
FT                   /evidence="ECO:0007829|PDB:7QN5"
SQ   SEQUENCE   554 AA;  61623 MW;  B159F9934FB02390 CRC64;
     MVSAKKVPAI ALSAGVSFAL LRFLCLAVCL NESPGQNQKE EKLCTENFTR ILDSLLDGYD
     NRLRPGFGGP VTEVKTDIYV TSFGPVSDVE MEYTMDVFFR QTWIDKRLKY DGPIEILRLN
     NMMVTKVWTP DTFFRNGKKS VSHNMTAPNK LFRIMRNGTI LYTMRLTISA ECPMRLVDFP
     MDGHACPLKF GSYAYPKSEM IYTWTKGPEK SVEVPKESSS LVQYDLIGQT VSSETIKSIT
     GEYIVMTVYF HLRRKMGYFM IQTYIPCIMT VILSQVSFWI NKESVPARTV FGITTVLTMT
     TLSISARHSL PKVSYATAMD WFIAVCFAFV FSALIEFAAV NYFTNIQMEK AKRKTSKPPQ
     EVPAAPVQRE KHPEAPLQNT NANLNMRKRT NALVHSESDV GNRTEVGNHS SKSSTVVQES
     SKGTPRSYLA SSPNPFSRAN AAETISAARA LPSASPTSIR TGYMPRKASV GSASTRHVFG
     SRLQRIKTTV NTIGATGKLS ATPPPSAPPP SGSGTSKIDK YARILFPVTF GAFNMVYWVV
     YLSKDTMEKS ESLM
//