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Protein

NADP-dependent malic enzyme

Gene

ME1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations. Prefers magnesium or manganese.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei102 – 1021Proton donorBy similarity
Binding sitei155 – 1551NADPBy similarity
Active sitei173 – 1731Proton acceptorBy similarity
Metal bindingi245 – 2451Divalent metal cationBy similarity
Metal bindingi246 – 2461Divalent metal cationBy similarity
Metal bindingi269 – 2691Divalent metal cationBy similarity
Binding sitei269 – 2691NADPBy similarity
Sitei269 – 2691Important for activityBy similarity
Binding sitei408 – 4081NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi301 – 31818NADPBy similarityAdd
BLAST

GO - Molecular functioni

  • ADP binding Source: UniProtKB
  • electron carrier activity Source: UniProtKB
  • malate dehydrogenase (decarboxylating) (NAD+) activity Source: InterPro
  • malate dehydrogenase (decarboxylating) (NADP+) activity Source: UniProtKB
  • malic enzyme activity Source: UniProtKB
  • manganese ion binding Source: UniProtKB
  • NAD binding Source: UniProtKB
  • NADP binding Source: UniProtKB
  • oxaloacetate decarboxylase activity Source: UniProtKB-EC

GO - Biological processi

  • carbohydrate metabolic process Source: ProtInc
  • malate metabolic process Source: UniProtKB
  • NADP biosynthetic process Source: UniProtKB
  • protein tetramerization Source: Ensembl
  • regulation of NADP metabolic process Source: CACAO
  • response to carbohydrate Source: UniProtKB
  • response to hormone Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-HSA-1989781. PPARA activates gene expression.
SABIO-RKP48163.

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-dependent malic enzyme (EC:1.1.1.40)
Short name:
NADP-ME
Alternative name(s):
Malic enzyme 1
Gene namesi
Name:ME1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:6983. ME1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30723.

Chemistry

ChEMBLiCHEMBL3495.

Polymorphism and mutation databases

BioMutaiME1.
DMDMi1346484.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572NADP-dependent malic enzymePRO_0000160192Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources
Modified residuei336 – 3361PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP48163.
MaxQBiP48163.
PaxDbiP48163.
PeptideAtlasiP48163.
PRIDEiP48163.

2D gel databases

REPRODUCTION-2DPAGEIPI00008215.

PTM databases

iPTMnetiP48163.
PhosphoSiteiP48163.
SwissPalmiP48163.

Expressioni

Tissue specificityi

Expressed in all tissues tested including liver, placenta and white adipose tissue.2 Publications

Gene expression databases

BgeeiENSG00000065833.
CleanExiHS_ME1.
GenevisibleiP48163. HS.

Organism-specific databases

HPAiHPA006493.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi110363. 29 interactions.
MINTiMINT-5004289.
STRINGi9606.ENSP00000358719.

Chemistry

BindingDBiP48163.

Structurei

Secondary structure

1
572
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi16 – 205Combined sources
Turni22 – 243Combined sources
Helixi27 – 293Combined sources
Helixi32 – 376Combined sources
Turni41 – 433Combined sources
Helixi51 – 6414Combined sources
Helixi68 – 8114Combined sources
Helixi83 – 919Combined sources
Turni95 – 973Combined sources
Helixi98 – 1014Combined sources
Helixi105 – 1117Combined sources
Helixi113 – 1164Combined sources
Beta strandi122 – 1265Combined sources
Helixi127 – 1293Combined sources
Helixi133 – 1375Combined sources
Beta strandi147 – 1515Combined sources
Turni157 – 1593Combined sources
Helixi163 – 1675Combined sources
Helixi168 – 18114Combined sources
Helixi185 – 1873Combined sources
Beta strandi188 – 1947Combined sources
Helixi200 – 2045Combined sources
Helixi218 – 23619Combined sources
Beta strandi241 – 2444Combined sources
Helixi249 – 25911Combined sources
Turni260 – 2623Combined sources
Beta strandi263 – 2675Combined sources
Turni268 – 2703Combined sources
Helixi271 – 28818Combined sources
Helixi292 – 2943Combined sources
Beta strandi297 – 3015Combined sources
Helixi304 – 32017Combined sources
Helixi324 – 3285Combined sources
Beta strandi331 – 3355Combined sources
Helixi351 – 3544Combined sources
Helixi364 – 3718Combined sources
Beta strandi374 – 3785Combined sources
Helixi388 – 39710Combined sources
Beta strandi402 – 4054Combined sources
Helixi410 – 4123Combined sources
Helixi417 – 4237Combined sources
Turni424 – 4263Combined sources
Beta strandi429 – 4346Combined sources
Helixi454 – 4563Combined sources
Helixi458 – 46811Combined sources
Helixi475 – 48713Combined sources
Helixi491 – 4955Combined sources
Helixi503 – 5053Combined sources
Helixi506 – 52318Combined sources
Helixi536 – 5427Combined sources
Helixi561 – 5644Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AW5X-ray2.50A/B/C13-564[»]
3WJAX-ray2.55A/B1-572[»]
ProteinModelPortaliP48163.
SMRiP48163. Positions 8-570.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP48163.

Family & Domainsi

Sequence similaritiesi

Belongs to the malic enzymes family.Curated

Phylogenomic databases

eggNOGiKOG1257. Eukaryota.
COG0281. LUCA.
GeneTreeiENSGT00390000000754.
HOVERGENiHBG000746.
InParanoidiP48163.
KOiK00029.
OMAiLNDPMYM.
OrthoDBiEOG091G04H9.
PhylomeDBiP48163.
TreeFamiTF300537.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM01274. malic. 1 hit.
SM00919. Malic_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P48163-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPEAPRRRH THQRGYLLTR NPHLNKDLAF TLEERQQLNI HGLLPPSFNS
60 70 80 90 100
QEIQVLRVVK NFEHLNSDFD RYLLLMDLQD RNEKLFYRVL TSDIEKFMPI
110 120 130 140 150
VYTPTVGLAC QQYSLVFRKP RGLFITIHDR GHIASVLNAW PEDVIKAIVV
160 170 180 190 200
TDGERILGLG DLGCNGMGIP VGKLALYTAC GGMNPQECLP VILDVGTENE
210 220 230 240 250
ELLKDPLYIG LRQRRVRGSE YDDFLDEFME AVSSKYGMNC LIQFEDFANV
260 270 280 290 300
NAFRLLNKYR NQYCTFNDDI QGTASVAVAG LLAALRITKN KLSDQTILFQ
310 320 330 340 350
GAGEAALGIA HLIVMALEKE GLPKEKAIKK IWLVDSKGLI VKGRASLTQE
360 370 380 390 400
KEKFAHEHEE MKNLEAIVQE IKPTALIGVA AIGGAFSEQI LKDMAAFNER
410 420 430 440 450
PIIFALSNPT SKAECSAEQC YKITKGRAIF ASGSPFDPVT LPNGQTLYPG
460 470 480 490 500
QGNNSYVFPG VALGVVACGL RQITDNIFLT TAEVIAQQVS DKHLEEGRLY
510 520 530 540 550
PPLNTIRDVS LKIAEKIVKD AYQEKTATVY PEPQNKEAFV RSQMYSTDYD
560 570
QILPDCYSWP EEVQKIQTKV DQ
Length:572
Mass (Da):64,150
Last modified:February 1, 1996 - v1
Checksum:iEA4C8CB36F6C619C
GO
Isoform 2 (identifier: P48163-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-75: Missing.

Note: No experimental confirmation available.
Show »
Length:497
Mass (Da):55,165
Checksum:i93AA2479DBD4EB40
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti266 – 2661F → S in BAD97137 (Ref. 4) Curated
Sequence conflicti438 – 4381P → S in AAC50613 (PubMed:8804575).Curated
Sequence conflicti443 – 4486NGQTLY → DGRTLF in AAB01380 (PubMed:7622060).Curated
Sequence conflicti472 – 4776QITDNI → HIDDKV in AAB01380 (PubMed:7622060).Curated
Sequence conflicti486 – 4861A → S in AAB01380 (PubMed:7622060).Curated
Sequence conflicti495 – 4951E → Q in AAB01380 (PubMed:7622060).Curated
Sequence conflicti515 – 5151E → V in AAB01380 (PubMed:7622060).Curated
Sequence conflicti519 – 5191K → Q in AAB01380 (PubMed:7622060).Curated
Sequence conflicti523 – 5231Q → K in AAB01380 (PubMed:7622060).Curated
Sequence conflicti526 – 5261T → M in AAB01380 (PubMed:7622060).Curated
Sequence conflicti538 – 5381A → E in AAB01380 (PubMed:7622060).Curated
Sequence conflicti541 – 5411R → S in AAB01380 (PubMed:7622060).Curated
Sequence conflicti548 – 5481D → N in AAB01380 (PubMed:7622060).Curated
Sequence conflicti558 – 5581S → P in AAB01380 (PubMed:7622060).Curated
Sequence conflicti561 – 5611E → A in AAB01380 (PubMed:7622060).Curated
Sequence conflicti571 – 5711D → N in AAB01380 (PubMed:7622060).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7575Missing in isoform 2. 1 PublicationVSP_057051Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77244 mRNA. Translation: CAA54460.1.
L34035 mRNA. Translation: AAB01380.1.
AK302777 mRNA. Translation: BAG63982.1.
AK223417 mRNA. Translation: BAD97137.1.
AL391416, AL049699, AL136970 Genomic DNA. Translation: CAH73129.1.
AL049699, AL136970, AL391416 Genomic DNA. Translation: CAI22634.1.
AL136970, AL049699, AL391416 Genomic DNA. Translation: CAC19505.2.
BC025246 mRNA. Translation: AAH25246.1.
U43944 mRNA. Translation: AAC50613.1.
CCDSiCCDS34492.1. [P48163-1]
PIRiJC4160.
S44415.
RefSeqiNP_002386.1. NM_002395.5. [P48163-1]
XP_011534138.1. XM_011535836.2. [P48163-2]
UniGeneiHs.21160.

Genome annotation databases

EnsembliENST00000369705; ENSP00000358719; ENSG00000065833. [P48163-1]
GeneIDi4199.
KEGGihsa:4199.
UCSCiuc003pjy.4. human. [P48163-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

SHMPD

The Singapore human mutation and polymorphism database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77244 mRNA. Translation: CAA54460.1.
L34035 mRNA. Translation: AAB01380.1.
AK302777 mRNA. Translation: BAG63982.1.
AK223417 mRNA. Translation: BAD97137.1.
AL391416, AL049699, AL136970 Genomic DNA. Translation: CAH73129.1.
AL049699, AL136970, AL391416 Genomic DNA. Translation: CAI22634.1.
AL136970, AL049699, AL391416 Genomic DNA. Translation: CAC19505.2.
BC025246 mRNA. Translation: AAH25246.1.
U43944 mRNA. Translation: AAC50613.1.
CCDSiCCDS34492.1. [P48163-1]
PIRiJC4160.
S44415.
RefSeqiNP_002386.1. NM_002395.5. [P48163-1]
XP_011534138.1. XM_011535836.2. [P48163-2]
UniGeneiHs.21160.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AW5X-ray2.50A/B/C13-564[»]
3WJAX-ray2.55A/B1-572[»]
ProteinModelPortaliP48163.
SMRiP48163. Positions 8-570.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110363. 29 interactions.
MINTiMINT-5004289.
STRINGi9606.ENSP00000358719.

Chemistry

BindingDBiP48163.
ChEMBLiCHEMBL3495.

PTM databases

iPTMnetiP48163.
PhosphoSiteiP48163.
SwissPalmiP48163.

Polymorphism and mutation databases

BioMutaiME1.
DMDMi1346484.

2D gel databases

REPRODUCTION-2DPAGEIPI00008215.

Proteomic databases

EPDiP48163.
MaxQBiP48163.
PaxDbiP48163.
PeptideAtlasiP48163.
PRIDEiP48163.

Protocols and materials databases

DNASUi4199.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369705; ENSP00000358719; ENSG00000065833. [P48163-1]
GeneIDi4199.
KEGGihsa:4199.
UCSCiuc003pjy.4. human. [P48163-1]

Organism-specific databases

CTDi4199.
GeneCardsiME1.
HGNCiHGNC:6983. ME1.
HPAiHPA006493.
MIMi154250. gene.
neXtProtiNX_P48163.
PharmGKBiPA30723.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1257. Eukaryota.
COG0281. LUCA.
GeneTreeiENSGT00390000000754.
HOVERGENiHBG000746.
InParanoidiP48163.
KOiK00029.
OMAiLNDPMYM.
OrthoDBiEOG091G04H9.
PhylomeDBiP48163.
TreeFamiTF300537.

Enzyme and pathway databases

ReactomeiR-HSA-1989781. PPARA activates gene expression.
SABIO-RKP48163.

Miscellaneous databases

EvolutionaryTraceiP48163.
GeneWikiiME1.
ME1_(gene).
GenomeRNAii4199.
PROiP48163.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000065833.
CleanExiHS_ME1.
GenevisibleiP48163. HS.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM01274. malic. 1 hit.
SM00919. Malic_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAOX_HUMAN
AccessioniPrimary (citable) accession number: P48163
Secondary accession number(s): B4DZ70
, Q16797, Q16855, Q53F72, Q5VWA2, Q9BWX8, Q9H1W3, Q9UIY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 7, 2016
This is version 163 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.