Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADP-dependent malic enzyme

Gene

ME1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations. Prefers magnesium or manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102Proton donorBy similarity1
Binding sitei155NADPBy similarity1
Active sitei173Proton acceptorBy similarity1
Metal bindingi245Divalent metal cationBy similarity1
Metal bindingi246Divalent metal cationBy similarity1
Metal bindingi269Divalent metal cationBy similarity1
Binding sitei269NADPBy similarity1
Sitei269Important for activityBy similarity1
Binding sitei408NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi301 – 318NADPBy similarityAdd BLAST18

GO - Molecular functioni

  • ADP binding Source: UniProtKB
  • electron transfer activity Source: UniProtKB
  • malate dehydrogenase (decarboxylating) (NAD+) activity Source: InterPro
  • malate dehydrogenase (decarboxylating) (NADP+) activity Source: UniProtKB
  • malic enzyme activity Source: UniProtKB
  • manganese ion binding Source: UniProtKB
  • NAD binding Source: UniProtKB
  • NADP binding Source: UniProtKB
  • oxaloacetate decarboxylase activity Source: UniProtKB-EC

GO - Biological processi

  • carbohydrate metabolic process Source: ProtInc
  • malate metabolic process Source: UniProtKB
  • NADP biosynthetic process Source: UniProtKB
  • protein tetramerization Source: Ensembl
  • pyruvate metabolic process Source: GO_Central
  • regulation of lipid metabolic process Source: Reactome
  • regulation of NADP metabolic process Source: CACAO
  • response to carbohydrate Source: UniProtKB
  • response to hormone Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NADP

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-70268 Pyruvate metabolism
SABIO-RKiP48163

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-dependent malic enzyme (EC:1.1.1.40)
Short name:
NADP-ME
Alternative name(s):
Malic enzyme 1
Gene namesi
Name:ME1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000065833.8
HGNCiHGNC:6983 ME1
MIMi154250 gene
neXtProtiNX_P48163

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi4199
OpenTargetsiENSG00000065833
PharmGKBiPA30723

Chemistry databases

ChEMBLiCHEMBL3495
DrugBankiDB03461 2'-Monophosphoadenosine 5'-Diphosphoribose
DB00157 NADH

Polymorphism and mutation databases

BioMutaiME1
DMDMi1346484

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001601921 – 572NADP-dependent malic enzymeAdd BLAST572

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei336PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP48163
MaxQBiP48163
PaxDbiP48163
PeptideAtlasiP48163
PRIDEiP48163

2D gel databases

REPRODUCTION-2DPAGEiIPI00008215

PTM databases

iPTMnetiP48163
PhosphoSitePlusiP48163
SwissPalmiP48163

Expressioni

Tissue specificityi

Expressed in all tissues tested including liver, placenta and white adipose tissue.2 Publications

Gene expression databases

BgeeiENSG00000065833
CleanExiHS_ME1
GenevisibleiP48163 HS

Organism-specific databases

HPAiHPA006493

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi110363, 31 interactors
STRINGi9606.ENSP00000358719

Chemistry databases

BindingDBiP48163

Structurei

Secondary structure

1572
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 20Combined sources5
Turni22 – 24Combined sources3
Helixi27 – 29Combined sources3
Helixi32 – 37Combined sources6
Turni41 – 43Combined sources3
Helixi51 – 64Combined sources14
Helixi68 – 81Combined sources14
Helixi83 – 91Combined sources9
Turni95 – 97Combined sources3
Helixi98 – 101Combined sources4
Helixi105 – 111Combined sources7
Helixi113 – 116Combined sources4
Beta strandi122 – 126Combined sources5
Helixi127 – 129Combined sources3
Helixi133 – 137Combined sources5
Beta strandi147 – 151Combined sources5
Turni157 – 159Combined sources3
Helixi163 – 167Combined sources5
Helixi168 – 181Combined sources14
Helixi185 – 187Combined sources3
Beta strandi188 – 194Combined sources7
Helixi200 – 204Combined sources5
Helixi218 – 236Combined sources19
Beta strandi241 – 244Combined sources4
Helixi249 – 259Combined sources11
Turni260 – 262Combined sources3
Beta strandi263 – 267Combined sources5
Turni268 – 270Combined sources3
Helixi271 – 288Combined sources18
Helixi292 – 294Combined sources3
Beta strandi297 – 301Combined sources5
Helixi304 – 320Combined sources17
Helixi324 – 328Combined sources5
Beta strandi331 – 335Combined sources5
Helixi351 – 354Combined sources4
Helixi364 – 371Combined sources8
Beta strandi374 – 378Combined sources5
Helixi388 – 397Combined sources10
Beta strandi402 – 405Combined sources4
Helixi410 – 412Combined sources3
Helixi417 – 423Combined sources7
Turni424 – 426Combined sources3
Beta strandi429 – 434Combined sources6
Helixi454 – 456Combined sources3
Helixi458 – 468Combined sources11
Helixi475 – 487Combined sources13
Helixi491 – 495Combined sources5
Helixi503 – 505Combined sources3
Helixi506 – 523Combined sources18
Helixi536 – 542Combined sources7
Helixi561 – 564Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AW5X-ray2.50A/B/C13-564[»]
3WJAX-ray2.55A/B1-572[»]
ProteinModelPortaliP48163
SMRiP48163
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP48163

Family & Domainsi

Sequence similaritiesi

Belongs to the malic enzymes family.Curated

Phylogenomic databases

eggNOGiKOG1257 Eukaryota
COG0281 LUCA
GeneTreeiENSGT00390000000754
HOVERGENiHBG000746
InParanoidiP48163
KOiK00029
OMAiSSKYGMN
OrthoDBiEOG091G04H9
PhylomeDBiP48163
TreeFamiTF300537

Family and domain databases

Gene3Di3.40.50.10380, 1 hit
InterProiView protein in InterPro
IPR015884 Malic_enzyme_CS
IPR012301 Malic_N_dom
IPR037062 Malic_N_dom_sf
IPR012302 Malic_NAD-bd
IPR001891 Malic_OxRdtase
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00390 malic, 1 hit
PF03949 Malic_M, 1 hit
PIRSFiPIRSF000106 ME, 1 hit
PRINTSiPR00072 MALOXRDTASE
SMARTiView protein in SMART
SM01274 malic, 1 hit
SM00919 Malic_M, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00331 MALIC_ENZYMES, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P48163-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPEAPRRRH THQRGYLLTR NPHLNKDLAF TLEERQQLNI HGLLPPSFNS
60 70 80 90 100
QEIQVLRVVK NFEHLNSDFD RYLLLMDLQD RNEKLFYRVL TSDIEKFMPI
110 120 130 140 150
VYTPTVGLAC QQYSLVFRKP RGLFITIHDR GHIASVLNAW PEDVIKAIVV
160 170 180 190 200
TDGERILGLG DLGCNGMGIP VGKLALYTAC GGMNPQECLP VILDVGTENE
210 220 230 240 250
ELLKDPLYIG LRQRRVRGSE YDDFLDEFME AVSSKYGMNC LIQFEDFANV
260 270 280 290 300
NAFRLLNKYR NQYCTFNDDI QGTASVAVAG LLAALRITKN KLSDQTILFQ
310 320 330 340 350
GAGEAALGIA HLIVMALEKE GLPKEKAIKK IWLVDSKGLI VKGRASLTQE
360 370 380 390 400
KEKFAHEHEE MKNLEAIVQE IKPTALIGVA AIGGAFSEQI LKDMAAFNER
410 420 430 440 450
PIIFALSNPT SKAECSAEQC YKITKGRAIF ASGSPFDPVT LPNGQTLYPG
460 470 480 490 500
QGNNSYVFPG VALGVVACGL RQITDNIFLT TAEVIAQQVS DKHLEEGRLY
510 520 530 540 550
PPLNTIRDVS LKIAEKIVKD AYQEKTATVY PEPQNKEAFV RSQMYSTDYD
560 570
QILPDCYSWP EEVQKIQTKV DQ
Length:572
Mass (Da):64,150
Last modified:February 1, 1996 - v1
Checksum:iEA4C8CB36F6C619C
GO
Isoform 2 (identifier: P48163-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-75: Missing.

Note: No experimental confirmation available.
Show »
Length:497
Mass (Da):55,165
Checksum:i93AA2479DBD4EB40
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti266F → S in BAD97137 (Ref. 4) Curated1
Sequence conflicti438P → S in AAC50613 (PubMed:8804575).Curated1
Sequence conflicti443 – 448NGQTLY → DGRTLF in AAB01380 (PubMed:7622060).Curated6
Sequence conflicti472 – 477QITDNI → HIDDKV in AAB01380 (PubMed:7622060).Curated6
Sequence conflicti486A → S in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti495E → Q in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti515E → V in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti519K → Q in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti523Q → K in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti526T → M in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti538A → E in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti541R → S in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti548D → N in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti558S → P in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti561E → A in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti571D → N in AAB01380 (PubMed:7622060).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0570511 – 75Missing in isoform 2. 1 PublicationAdd BLAST75

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77244 mRNA Translation: CAA54460.1
L34035 mRNA Translation: AAB01380.1
AK302777 mRNA Translation: BAG63982.1
AK223417 mRNA Translation: BAD97137.1
AL049699 Genomic DNA No translation available.
AL136970 Genomic DNA No translation available.
AL391416 Genomic DNA No translation available.
BC025246 mRNA Translation: AAH25246.1
U43944 mRNA Translation: AAC50613.1
CCDSiCCDS34492.1 [P48163-1]
PIRiJC4160
S44415
RefSeqiNP_002386.1, NM_002395.5 [P48163-1]
XP_011534138.1, XM_011535836.2 [P48163-2]
UniGeneiHs.21160

Genome annotation databases

EnsembliENST00000369705; ENSP00000358719; ENSG00000065833 [P48163-1]
GeneIDi4199
KEGGihsa:4199
UCSCiuc003pjy.4 human [P48163-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMAOX_HUMAN
AccessioniPrimary (citable) accession number: P48163
Secondary accession number(s): B4DZ70
, Q16797, Q16855, Q53F72, Q5VWA2, Q9BWX8, Q9H1W3, Q9UIY4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 177 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health