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Protein

NADP-dependent malic enzyme

Gene

ME1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations. Prefers magnesium or manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102Proton donorBy similarity1
Binding sitei155NADPBy similarity1
Active sitei173Proton acceptorBy similarity1
Metal bindingi245Divalent metal cationBy similarity1
Metal bindingi246Divalent metal cationBy similarity1
Metal bindingi269Divalent metal cationBy similarity1
Binding sitei269NADPBy similarity1
Sitei269Important for activityBy similarity1
Binding sitei408NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi301 – 318NADPBy similarityAdd BLAST18

GO - Molecular functioni

  • ADP binding Source: UniProtKB
  • electron carrier activity Source: UniProtKB
  • malate dehydrogenase (decarboxylating) (NAD+) activity Source: InterPro
  • malate dehydrogenase (decarboxylating) (NADP+) activity Source: UniProtKB
  • malic enzyme activity Source: UniProtKB
  • manganese ion binding Source: UniProtKB
  • NAD binding Source: UniProtKB
  • NADP binding Source: UniProtKB
  • oxaloacetate decarboxylase activity Source: UniProtKB-EC

GO - Biological processi

  • carbohydrate metabolic process Source: ProtInc
  • malate metabolic process Source: UniProtKB
  • NADP biosynthetic process Source: UniProtKB
  • protein tetramerization Source: Ensembl
  • regulation of NADP metabolic process Source: CACAO
  • response to carbohydrate Source: UniProtKB
  • response to hormone Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NADP

Enzyme and pathway databases

BioCyciZFISH:HS00856-MONOMER.
ReactomeiR-HSA-1989781. PPARA activates gene expression.
SABIO-RKP48163.

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-dependent malic enzyme (EC:1.1.1.40)
Short name:
NADP-ME
Alternative name(s):
Malic enzyme 1
Gene namesi
Name:ME1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:6983. ME1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi4199.
OpenTargetsiENSG00000065833.
PharmGKBiPA30723.

Chemistry databases

ChEMBLiCHEMBL3495.

Polymorphism and mutation databases

BioMutaiME1.
DMDMi1346484.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001601921 – 572NADP-dependent malic enzymeAdd BLAST572

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei336PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP48163.
MaxQBiP48163.
PaxDbiP48163.
PeptideAtlasiP48163.
PRIDEiP48163.

2D gel databases

REPRODUCTION-2DPAGEIPI00008215.

PTM databases

iPTMnetiP48163.
PhosphoSitePlusiP48163.
SwissPalmiP48163.

Expressioni

Tissue specificityi

Expressed in all tissues tested including liver, placenta and white adipose tissue.2 Publications

Gene expression databases

BgeeiENSG00000065833.
CleanExiHS_ME1.
GenevisibleiP48163. HS.

Organism-specific databases

HPAiHPA006493.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi110363. 29 interactors.
MINTiMINT-5004289.
STRINGi9606.ENSP00000358719.

Chemistry databases

BindingDBiP48163.

Structurei

Secondary structure

1572
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 20Combined sources5
Turni22 – 24Combined sources3
Helixi27 – 29Combined sources3
Helixi32 – 37Combined sources6
Turni41 – 43Combined sources3
Helixi51 – 64Combined sources14
Helixi68 – 81Combined sources14
Helixi83 – 91Combined sources9
Turni95 – 97Combined sources3
Helixi98 – 101Combined sources4
Helixi105 – 111Combined sources7
Helixi113 – 116Combined sources4
Beta strandi122 – 126Combined sources5
Helixi127 – 129Combined sources3
Helixi133 – 137Combined sources5
Beta strandi147 – 151Combined sources5
Turni157 – 159Combined sources3
Helixi163 – 167Combined sources5
Helixi168 – 181Combined sources14
Helixi185 – 187Combined sources3
Beta strandi188 – 194Combined sources7
Helixi200 – 204Combined sources5
Helixi218 – 236Combined sources19
Beta strandi241 – 244Combined sources4
Helixi249 – 259Combined sources11
Turni260 – 262Combined sources3
Beta strandi263 – 267Combined sources5
Turni268 – 270Combined sources3
Helixi271 – 288Combined sources18
Helixi292 – 294Combined sources3
Beta strandi297 – 301Combined sources5
Helixi304 – 320Combined sources17
Helixi324 – 328Combined sources5
Beta strandi331 – 335Combined sources5
Helixi351 – 354Combined sources4
Helixi364 – 371Combined sources8
Beta strandi374 – 378Combined sources5
Helixi388 – 397Combined sources10
Beta strandi402 – 405Combined sources4
Helixi410 – 412Combined sources3
Helixi417 – 423Combined sources7
Turni424 – 426Combined sources3
Beta strandi429 – 434Combined sources6
Helixi454 – 456Combined sources3
Helixi458 – 468Combined sources11
Helixi475 – 487Combined sources13
Helixi491 – 495Combined sources5
Helixi503 – 505Combined sources3
Helixi506 – 523Combined sources18
Helixi536 – 542Combined sources7
Helixi561 – 564Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AW5X-ray2.50A/B/C13-564[»]
3WJAX-ray2.55A/B1-572[»]
ProteinModelPortaliP48163.
SMRiP48163.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP48163.

Family & Domainsi

Sequence similaritiesi

Belongs to the malic enzymes family.Curated

Phylogenomic databases

eggNOGiKOG1257. Eukaryota.
COG0281. LUCA.
GeneTreeiENSGT00390000000754.
HOVERGENiHBG000746.
InParanoidiP48163.
KOiK00029.
OMAiLNDPMYM.
OrthoDBiEOG091G04H9.
PhylomeDBiP48163.
TreeFamiTF300537.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM01274. malic. 1 hit.
SM00919. Malic_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P48163-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPEAPRRRH THQRGYLLTR NPHLNKDLAF TLEERQQLNI HGLLPPSFNS
60 70 80 90 100
QEIQVLRVVK NFEHLNSDFD RYLLLMDLQD RNEKLFYRVL TSDIEKFMPI
110 120 130 140 150
VYTPTVGLAC QQYSLVFRKP RGLFITIHDR GHIASVLNAW PEDVIKAIVV
160 170 180 190 200
TDGERILGLG DLGCNGMGIP VGKLALYTAC GGMNPQECLP VILDVGTENE
210 220 230 240 250
ELLKDPLYIG LRQRRVRGSE YDDFLDEFME AVSSKYGMNC LIQFEDFANV
260 270 280 290 300
NAFRLLNKYR NQYCTFNDDI QGTASVAVAG LLAALRITKN KLSDQTILFQ
310 320 330 340 350
GAGEAALGIA HLIVMALEKE GLPKEKAIKK IWLVDSKGLI VKGRASLTQE
360 370 380 390 400
KEKFAHEHEE MKNLEAIVQE IKPTALIGVA AIGGAFSEQI LKDMAAFNER
410 420 430 440 450
PIIFALSNPT SKAECSAEQC YKITKGRAIF ASGSPFDPVT LPNGQTLYPG
460 470 480 490 500
QGNNSYVFPG VALGVVACGL RQITDNIFLT TAEVIAQQVS DKHLEEGRLY
510 520 530 540 550
PPLNTIRDVS LKIAEKIVKD AYQEKTATVY PEPQNKEAFV RSQMYSTDYD
560 570
QILPDCYSWP EEVQKIQTKV DQ
Length:572
Mass (Da):64,150
Last modified:February 1, 1996 - v1
Checksum:iEA4C8CB36F6C619C
GO
Isoform 2 (identifier: P48163-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-75: Missing.

Note: No experimental confirmation available.
Show »
Length:497
Mass (Da):55,165
Checksum:i93AA2479DBD4EB40
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti266F → S in BAD97137 (Ref. 4) Curated1
Sequence conflicti438P → S in AAC50613 (PubMed:8804575).Curated1
Sequence conflicti443 – 448NGQTLY → DGRTLF in AAB01380 (PubMed:7622060).Curated6
Sequence conflicti472 – 477QITDNI → HIDDKV in AAB01380 (PubMed:7622060).Curated6
Sequence conflicti486A → S in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti495E → Q in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti515E → V in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti519K → Q in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti523Q → K in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti526T → M in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti538A → E in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti541R → S in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti548D → N in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti558S → P in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti561E → A in AAB01380 (PubMed:7622060).Curated1
Sequence conflicti571D → N in AAB01380 (PubMed:7622060).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0570511 – 75Missing in isoform 2. 1 PublicationAdd BLAST75

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77244 mRNA. Translation: CAA54460.1.
L34035 mRNA. Translation: AAB01380.1.
AK302777 mRNA. Translation: BAG63982.1.
AK223417 mRNA. Translation: BAD97137.1.
AL391416, AL049699, AL136970 Genomic DNA. Translation: CAH73129.1.
AL049699, AL136970, AL391416 Genomic DNA. Translation: CAI22634.1.
AL136970, AL049699, AL391416 Genomic DNA. Translation: CAC19505.2.
BC025246 mRNA. Translation: AAH25246.1.
U43944 mRNA. Translation: AAC50613.1.
CCDSiCCDS34492.1. [P48163-1]
PIRiJC4160.
S44415.
RefSeqiNP_002386.1. NM_002395.5. [P48163-1]
XP_011534138.1. XM_011535836.2. [P48163-2]
UniGeneiHs.21160.

Genome annotation databases

EnsembliENST00000369705; ENSP00000358719; ENSG00000065833. [P48163-1]
GeneIDi4199.
KEGGihsa:4199.
UCSCiuc003pjy.4. human. [P48163-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

SHMPD

The Singapore human mutation and polymorphism database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77244 mRNA. Translation: CAA54460.1.
L34035 mRNA. Translation: AAB01380.1.
AK302777 mRNA. Translation: BAG63982.1.
AK223417 mRNA. Translation: BAD97137.1.
AL391416, AL049699, AL136970 Genomic DNA. Translation: CAH73129.1.
AL049699, AL136970, AL391416 Genomic DNA. Translation: CAI22634.1.
AL136970, AL049699, AL391416 Genomic DNA. Translation: CAC19505.2.
BC025246 mRNA. Translation: AAH25246.1.
U43944 mRNA. Translation: AAC50613.1.
CCDSiCCDS34492.1. [P48163-1]
PIRiJC4160.
S44415.
RefSeqiNP_002386.1. NM_002395.5. [P48163-1]
XP_011534138.1. XM_011535836.2. [P48163-2]
UniGeneiHs.21160.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AW5X-ray2.50A/B/C13-564[»]
3WJAX-ray2.55A/B1-572[»]
ProteinModelPortaliP48163.
SMRiP48163.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110363. 29 interactors.
MINTiMINT-5004289.
STRINGi9606.ENSP00000358719.

Chemistry databases

BindingDBiP48163.
ChEMBLiCHEMBL3495.

PTM databases

iPTMnetiP48163.
PhosphoSitePlusiP48163.
SwissPalmiP48163.

Polymorphism and mutation databases

BioMutaiME1.
DMDMi1346484.

2D gel databases

REPRODUCTION-2DPAGEIPI00008215.

Proteomic databases

EPDiP48163.
MaxQBiP48163.
PaxDbiP48163.
PeptideAtlasiP48163.
PRIDEiP48163.

Protocols and materials databases

DNASUi4199.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369705; ENSP00000358719; ENSG00000065833. [P48163-1]
GeneIDi4199.
KEGGihsa:4199.
UCSCiuc003pjy.4. human. [P48163-1]

Organism-specific databases

CTDi4199.
DisGeNETi4199.
GeneCardsiME1.
HGNCiHGNC:6983. ME1.
HPAiHPA006493.
MIMi154250. gene.
neXtProtiNX_P48163.
OpenTargetsiENSG00000065833.
PharmGKBiPA30723.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1257. Eukaryota.
COG0281. LUCA.
GeneTreeiENSGT00390000000754.
HOVERGENiHBG000746.
InParanoidiP48163.
KOiK00029.
OMAiLNDPMYM.
OrthoDBiEOG091G04H9.
PhylomeDBiP48163.
TreeFamiTF300537.

Enzyme and pathway databases

BioCyciZFISH:HS00856-MONOMER.
ReactomeiR-HSA-1989781. PPARA activates gene expression.
SABIO-RKP48163.

Miscellaneous databases

EvolutionaryTraceiP48163.
GeneWikiiME1.
ME1_(gene).
GenomeRNAii4199.
PROiP48163.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000065833.
CleanExiHS_ME1.
GenevisibleiP48163. HS.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM01274. malic. 1 hit.
SM00919. Malic_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAOX_HUMAN
AccessioniPrimary (citable) accession number: P48163
Secondary accession number(s): B4DZ70
, Q16797, Q16855, Q53F72, Q5VWA2, Q9BWX8, Q9H1W3, Q9UIY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 165 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.