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P48146

- NPBW2_HUMAN

UniProt

P48146 - NPBW2_HUMAN

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Protein

Neuropeptides B/W receptor type 2

Gene

NPBWR2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals.

GO - Molecular functioni

  1. neuropeptide receptor activity Source: InterPro
  2. opioid receptor activity Source: ProtInc

GO - Biological processi

  1. G-protein coupled receptor signaling pathway Source: HGNC
  2. opioid receptor signaling pathway Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_14819. Peptide ligand-binding receptors.
REACT_19231. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuropeptides B/W receptor type 2
Alternative name(s):
G-protein coupled receptor 8
Gene namesi
Name:NPBWR2
Synonyms:GPR8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 20

Organism-specific databases

HGNCiHGNC:4530. NPBWR2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4545ExtracellularSequence AnalysisAdd
BLAST
Transmembranei46 – 6924Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini70 – 8011CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei81 – 10525Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini106 – 12015ExtracellularSequence AnalysisAdd
BLAST
Transmembranei121 – 14020Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini141 – 16525CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei166 – 18520Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini186 – 21126ExtracellularSequence AnalysisAdd
BLAST
Transmembranei212 – 23322Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini234 – 25724CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei258 – 28225Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini283 – 29210ExtracellularSequence Analysis
Transmembranei293 – 30715Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini308 – 33326CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: HGNC
  2. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28923.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 333333Neuropeptides B/W receptor type 2PRO_0000069522Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi29 – 291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi117 ↔ 197PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP48146.
PRIDEiP48146.

PTM databases

PhosphoSiteiP48146.

Expressioni

Tissue specificityi

Detected at high levels in caudate nucleus, hyppocampus and amygdala; at moderate levels in the adult brain, thalamus, parietal cortex, pituitary gland, adrenal gland and lymph nodes.1 Publication

Gene expression databases

BgeeiP48146.
CleanExiHS_NPBWR2.
GenevestigatoriP48146.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000358783.

Structurei

3D structure databases

ProteinModelPortaliP48146.
SMRiP48146. Positions 9-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG283184.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230485.
HOVERGENiHBG106919.
InParanoidiP48146.
KOiK08377.
OMAiPERFWFK.
OrthoDBiEOG7BKCVQ.
PhylomeDBiP48146.
TreeFamiTF315737.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR009150. Neuropept_B/W_rcpt.
IPR027349. NPBW2.
[Graphical view]
PANTHERiPTHR24229:SF18. PTHR24229:SF18. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01855. NRPEPTIDEWR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48146-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQAAGHPEPL DSRGSFSLPT MGANVSQDNG TGHNATFSEP LPFLYVLLPA
60 70 80 90 100
VYSGICAVGL TGNTAVILVI LRAPKMKTVT NVFILNLAVA DGLFTLVLPV
110 120 130 140 150
NIAEHLLQYW PFGELLCKLV LAVDHYNIFS SIYFLAVMSV DRYLVVLATV
160 170 180 190 200
RSRHMPWRTY RGAKVASLCV WLGVTVLVLP FFSFAGVYSN ELQVPSCGLS
210 220 230 240 250
FPWPEQVWFK ASRVYTLVLG FVLPVCTICV LYTDLLRRLR AVRLRSGAKA
260 270 280 290 300
LGKARRKVTV LVLVVLAVCL LCWTPFHLAS VVALTTDLPQ TPLVISMSYV
310 320 330
ITSLSYANSC LNPFLYAFLD DNFRKNFRSI LRC
Length:333
Mass (Da):36,861
Last modified:November 13, 2007 - v2
Checksum:i8C416473FD80B0BA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti305 – 3051S → T in AAC50198. (PubMed:7590751)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti92 – 921G → R in a colorectal cancer sample; somatic mutation. 1 Publication
VAR_035766
Natural varianti206 – 2061Q → R.2 Publications
Corresponds to variant rs4809401 [ dbSNP | Ensembl ].
VAR_003579

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22492 Genomic DNA. Translation: AAC50198.1.
AL121581 Genomic DNA. Translation: CAC17004.1.
CH471077 Genomic DNA. Translation: EAW75161.1.
BC067481 mRNA. Translation: AAH67481.1.
BC067482 mRNA. Translation: AAH67482.1.
CCDSiCCDS13557.1.
PIRiI38974.
RefSeqiNP_005277.2. NM_005286.2.
UniGeneiHs.248118.

Genome annotation databases

EnsembliENST00000369768; ENSP00000358783; ENSG00000125522.
ENST00000612646; ENSP00000484253; ENSG00000277339.
GeneIDi2832.
KEGGihsa:2832.
UCSCiuc011abt.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22492 Genomic DNA. Translation: AAC50198.1 .
AL121581 Genomic DNA. Translation: CAC17004.1 .
CH471077 Genomic DNA. Translation: EAW75161.1 .
BC067481 mRNA. Translation: AAH67481.1 .
BC067482 mRNA. Translation: AAH67482.1 .
CCDSi CCDS13557.1.
PIRi I38974.
RefSeqi NP_005277.2. NM_005286.2.
UniGenei Hs.248118.

3D structure databases

ProteinModelPortali P48146.
SMRi P48146. Positions 9-333.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000358783.

Chemistry

GuidetoPHARMACOLOGYi 304.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P48146.

Proteomic databases

PaxDbi P48146.
PRIDEi P48146.

Protocols and materials databases

DNASUi 2832.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000369768 ; ENSP00000358783 ; ENSG00000125522 .
ENST00000612646 ; ENSP00000484253 ; ENSG00000277339 .
GeneIDi 2832.
KEGGi hsa:2832.
UCSCi uc011abt.2. human.

Organism-specific databases

CTDi 2832.
GeneCardsi GC20M062737.
H-InvDB HIX0040594.
HGNCi HGNC:4530. NPBWR2.
MIMi 600731. gene.
neXtProti NX_P48146.
PharmGKBi PA28923.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG283184.
GeneTreei ENSGT00760000118797.
HOGENOMi HOG000230485.
HOVERGENi HBG106919.
InParanoidi P48146.
KOi K08377.
OMAi PERFWFK.
OrthoDBi EOG7BKCVQ.
PhylomeDBi P48146.
TreeFami TF315737.

Enzyme and pathway databases

Reactomei REACT_14819. Peptide ligand-binding receptors.
REACT_19231. G alpha (i) signalling events.

Miscellaneous databases

GeneWikii Neuropeptides_B/W_receptor_2.
GenomeRNAii 2832.
NextBioi 11177.
PROi P48146.
SOURCEi Search...

Gene expression databases

Bgeei P48146.
CleanExi HS_NPBWR2.
Genevestigatori P48146.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR009150. Neuropept_B/W_rcpt.
IPR027349. NPBW2.
[Graphical view ]
PANTHERi PTHR24229:SF18. PTHR24229:SF18. 1 hit.
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00237. GPCRRHODOPSN.
PR01855. NRPEPTIDEWR.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The cloning and chromosomal mapping of two novel human opioid-somatostatin-like receptor genes, GPR7 and GPR8, expressed in discrete areas of the brain."
    O'Dowd B.F., Scheideler M.A., Nguyen T., Cheng R., Rasmussen J.S., Marchese A., Zastawny R., Heng H.H.Q., Tsui L.-C., Shi X., Asa S., Puy L., George S.R.
    Genomics 28:84-91(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ARG-206.
  2. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Identification of natural ligands for the orphan G protein-coupled receptors GPR7 and GPR8."
    Brezillon S., Lannoy V., Franssen J.-D., Le Poul E., Dupriez V., Lucchetti J., Detheux M., Parmentier M.
    J. Biol. Chem. 278:776-783(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INTERACTION WITH NEUROPEPTIDES B AND W.
  6. Cited for: INTERACTION WITH NEUROPEPTIDES B AND W.
  7. "Identification of a novel amino acid substitution (R206Q) in the second extracellular loop of the opioid-somatostatin-like receptor gene GPR8."
    Zeng J., Liu M., Grau O., Capron A., Bahr G.M.
    Hum. Mutat. 12:219-219(1998)
    Cited for: VARIANT ARG-206.
  8. Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-92.

Entry informationi

Entry nameiNPBW2_HUMAN
AccessioniPrimary (citable) accession number: P48146
Secondary accession number(s): Q6NWQ6, Q9H4K3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 13, 2007
Last modified: November 26, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3