P48146 (NPBW2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuropeptides B/W receptor type 2

Alternative name(s):
G-protein coupled receptor 8

Gene names

Name:	NPBWR2
Synonyms:	GPR8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Detected at high levels in caudate nucleus, hyppocampus and amygdala; at moderate levels in the adult brain, thalamus, parietal cortex, pituitary gland, adrenal gland and lymph nodes. Ref.5
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	neuropeptide signaling pathway Inferred from electronic annotation. Source: GOC
Cellular_component	integral to membrane Inferred by curator Ref.5. Source: HGNC plasma membrane Traceable author statement. Source: Reactome
Molecular_function	neuropeptide receptor activity Inferred from electronic annotation. Source: InterPro opioid receptor activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Neuropeptides B/W receptor type 2		PRO_0000069522
Regions
	Topological domain	1 – 45	45	Extracellular Potential
	Transmembrane	46 – 69	24	Helical; Name=1; Potential
	Topological domain	70 – 80	11	Cytoplasmic Potential
	Transmembrane	81 – 105	25	Helical; Name=2; Potential
	Topological domain	106 – 120	15	Extracellular Potential
	Transmembrane	121 – 140	20	Helical; Name=3; Potential
	Topological domain	141 – 165	25	Cytoplasmic Potential
	Transmembrane	166 – 185	20	Helical; Name=4; Potential
	Topological domain	186 – 211	26	Extracellular Potential
	Transmembrane	212 – 233	22	Helical; Name=5; Potential
	Topological domain	234 – 257	24	Cytoplasmic Potential
	Transmembrane	258 – 282	25	Helical; Name=6; Potential
	Topological domain	283 – 292	10	Extracellular Potential
	Transmembrane	293 – 307	15	Helical; Name=7; Potential
	Topological domain	308 – 333	26	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	34	1	N-linked (GlcNAc...) Potential
	Disulfide bond	117 ↔ 197		By similarity
Natural variations
	Natural variant	92	1	G → R in a colorectal cancer sample; somatic mutation. Ref.8		VAR_035766
	Natural variant	206	1	Q → R. Ref.1 Ref.7 Corresponds to variant rs4809401 [ dbSNP | Ensembl ].		VAR_003579
Experimental info
	Sequence conflict	305	1	S → T in AAC50198. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48146 [UniParc]. Last modified November 13, 2007. Version 2. Checksum: 8C416473FD80B0BA	FASTA	333	36,861
	10 20 30 40 50 60 MQAAGHPEPL DSRGSFSLPT MGANVSQDNG TGHNATFSEP LPFLYVLLPA VYSGICAVGL 70 80 90 100 110 120 TGNTAVILVI LRAPKMKTVT NVFILNLAVA DGLFTLVLPV NIAEHLLQYW PFGELLCKLV 130 140 150 160 170 180 LAVDHYNIFS SIYFLAVMSV DRYLVVLATV RSRHMPWRTY RGAKVASLCV WLGVTVLVLP 190 200 210 220 230 240 FFSFAGVYSN ELQVPSCGLS FPWPEQVWFK ASRVYTLVLG FVLPVCTICV LYTDLLRRLR 250 260 270 280 290 300 AVRLRSGAKA LGKARRKVTV LVLVVLAVCL LCWTPFHLAS VVALTTDLPQ TPLVISMSYV 310 320 330 ITSLSYANSC LNPFLYAFLD DNFRKNFRSI LRC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cloning and chromosomal mapping of two novel human opioid-somatostatin-like receptor genes, GPR7 and GPR8, expressed in discrete areas of the brain." O'Dowd B.F., Scheideler M.A., Nguyen T., Cheng R., Rasmussen J.S., Marchese A., Zastawny R., Heng H.H.Q., Tsui L.-C., Shi X., Asa S., Puy L., George S.R. Genomics 28:84-91(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ARG-206.
[2]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Identification of natural ligands for the orphan G protein-coupled receptors GPR7 and GPR8." Brezillon S., Lannoy V., Franssen J.-D., Le Poul E., Dupriez V., Lucchetti J., Detheux M., Parmentier M. J. Biol. Chem. 278:776-783(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INTERACTION WITH NEUROPEPTIDES B AND W.
[6]	"Characterization of a family of endogenous neuropeptide ligands for the G protein-coupled receptors GPR7 and GPR8." Tanaka H., Yoshida T., Miyamoto N., Motoike T., Kurosu H., Shibata K., Yamanaka A., Williams S.C., Richardson J.A., Tsujino N., Garry M.G., Lerner M.R., King D.S., O'Dowd B.F., Sakurai T., Yanagisawa M. Proc. Natl. Acad. Sci. U.S.A. 100:6251-6256(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NEUROPEPTIDES B AND W.
[7]	"Identification of a novel amino acid substitution (R206Q) in the second extracellular loop of the opioid-somatostatin-like receptor gene GPR8." Zeng J., Liu M., Grau O., Capron A., Bahr G.M. Hum. Mutat. 12:219-219(1998) Cited for: VARIANT ARG-206.
[8]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-92.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U22492 Genomic DNA. Translation: AAC50198.1. AL121581 Genomic DNA. Translation: CAC17004.1. CH471077 Genomic DNA. Translation: EAW75161.1. BC067481 mRNA. Translation: AAH67481.1. BC067482 mRNA. Translation: AAH67482.1.
IPI	IPI00008162.
PIR	I38974.
RefSeq	NP_005277.2. NM_005286.2.
UniGene	Hs.248118.
3D structure databases
ProteinModelPortal	P48146.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000358783.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P48146.
Polymorphism databases
DMDM	160332190.
Proteomic databases
PaxDb	P48146.
PRIDE	P48146.
Protocols and materials databases
DNASU	2832.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000369768; ENSP00000358783; ENSG00000125522.
GeneID	2832.
KEGG	hsa:2832.
UCSC	uc011abt.2. human.
Organism-specific databases
CTD	2832.
GeneCards	GC20M062737.
H-InvDB	HIX0040594.
HGNC	HGNC:4530. NPBWR2.
MIM	600731. gene.
neXtProt	NX_P48146.
PharmGKB	PA28923.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG283184.
HOGENOM	HOG000230485.
HOVERGEN	HBG106919.
InParanoid	P48146.
KO	K08377.
OMA	VYSNELQ.
OrthoDB	EOG49GKH1.
PhylomeDB	P48146.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	P48146.
CleanEx	HS_NPBWR2.
Genevestigator	P48146.
GermOnline	ENSG00000125522. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR009150. Neuropept_B/W_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01855. NRPEPTIDEWR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	2832.
NextBio	11177.
SOURCE	Search...

Entry information

Entry name

NPBW2_HUMAN

Accession

Primary (citable) accession number: P48146
Secondary accession number(s): Q6NWQ6, Q9H4K3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 125 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.