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Protein

Neuropeptides B/W receptor type 2

Gene

NPBWR2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals.

GO - Molecular functioni

  • neuropeptide binding Source: GO_Central
  • neuropeptide receptor activity Source: InterPro
  • opioid receptor activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_14819. Peptide ligand-binding receptors.
REACT_19231. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuropeptides B/W receptor type 2
Alternative name(s):
G-protein coupled receptor 8
Gene namesi
Name:NPBWR2
Synonyms:GPR8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:4530. NPBWR2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4545ExtracellularSequence AnalysisAdd
BLAST
Transmembranei46 – 6924Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini70 – 8011CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei81 – 10525Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini106 – 12015ExtracellularSequence AnalysisAdd
BLAST
Transmembranei121 – 14020Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini141 – 16525CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei166 – 18520Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini186 – 21126ExtracellularSequence AnalysisAdd
BLAST
Transmembranei212 – 23322Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini234 – 25724CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei258 – 28225Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini283 – 29210ExtracellularSequence Analysis
Transmembranei293 – 30715Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini308 – 33326CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28923.

Polymorphism and mutation databases

BioMutaiNPBWR2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 333333Neuropeptides B/W receptor type 2PRO_0000069522Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi29 – 291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi117 ↔ 197PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP48146.
PRIDEiP48146.

PTM databases

PhosphoSiteiP48146.

Expressioni

Tissue specificityi

Detected at high levels in caudate nucleus, hyppocampus and amygdala; at moderate levels in the adult brain, thalamus, parietal cortex, pituitary gland, adrenal gland and lymph nodes.1 Publication

Gene expression databases

BgeeiP48146.
CleanExiHS_NPBWR2.
GenevisibleiP48146. HS.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
BOLLQ8N9W63EBI-10210114,EBI-998198
KRT31Q153233EBI-10210114,EBI-948001
KRT40Q6A1623EBI-10210114,EBI-10171697
KRTAP10-7P604093EBI-10210114,EBI-10172290
KRTAP10-9P604113EBI-10210114,EBI-10172052
KRTAP5-9P263713EBI-10210114,EBI-3958099
NOTCH2NLQ7Z3S93EBI-10210114,EBI-945833
PNMA1Q8ND903EBI-10210114,EBI-302345
PSMA3P257883EBI-10210114,EBI-348380

Protein-protein interaction databases

BioGridi109093. 10 interactions.
IntActiP48146. 9 interactions.
STRINGi9606.ENSP00000358783.

Structurei

3D structure databases

ProteinModelPortaliP48146.
SMRiP48146. Positions 9-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG283184.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230485.
HOVERGENiHBG106919.
InParanoidiP48146.
KOiK08377.
OMAiPERFWFK.
OrthoDBiEOG7BKCVQ.
PhylomeDBiP48146.
TreeFamiTF315737.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR009150. Neuropept_B/W_rcpt.
IPR027349. NPBW2.
[Graphical view]
PANTHERiPTHR24229:SF18. PTHR24229:SF18. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01855. NRPEPTIDEWR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48146-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAAGHPEPL DSRGSFSLPT MGANVSQDNG TGHNATFSEP LPFLYVLLPA
60 70 80 90 100
VYSGICAVGL TGNTAVILVI LRAPKMKTVT NVFILNLAVA DGLFTLVLPV
110 120 130 140 150
NIAEHLLQYW PFGELLCKLV LAVDHYNIFS SIYFLAVMSV DRYLVVLATV
160 170 180 190 200
RSRHMPWRTY RGAKVASLCV WLGVTVLVLP FFSFAGVYSN ELQVPSCGLS
210 220 230 240 250
FPWPEQVWFK ASRVYTLVLG FVLPVCTICV LYTDLLRRLR AVRLRSGAKA
260 270 280 290 300
LGKARRKVTV LVLVVLAVCL LCWTPFHLAS VVALTTDLPQ TPLVISMSYV
310 320 330
ITSLSYANSC LNPFLYAFLD DNFRKNFRSI LRC
Length:333
Mass (Da):36,861
Last modified:November 13, 2007 - v2
Checksum:i8C416473FD80B0BA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti305 – 3051S → T in AAC50198 (PubMed:7590751).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti92 – 921G → R in a colorectal cancer sample; somatic mutation. 1 Publication
VAR_035766
Natural varianti206 – 2061Q → R.2 Publications
Corresponds to variant rs4809401 [ dbSNP | Ensembl ].
VAR_003579

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22492 Genomic DNA. Translation: AAC50198.1.
AL121581 Genomic DNA. Translation: CAC17004.1.
CH471077 Genomic DNA. Translation: EAW75161.1.
BC067481 mRNA. Translation: AAH67481.1.
BC067482 mRNA. Translation: AAH67482.1.
CCDSiCCDS13557.1.
PIRiI38974.
RefSeqiNP_005277.2. NM_005286.2.
UniGeneiHs.248118.

Genome annotation databases

EnsembliENST00000369768; ENSP00000358783; ENSG00000125522.
ENST00000612646; ENSP00000484253; ENSG00000277339.
GeneIDi2832.
KEGGihsa:2832.
UCSCiuc011abt.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22492 Genomic DNA. Translation: AAC50198.1.
AL121581 Genomic DNA. Translation: CAC17004.1.
CH471077 Genomic DNA. Translation: EAW75161.1.
BC067481 mRNA. Translation: AAH67481.1.
BC067482 mRNA. Translation: AAH67482.1.
CCDSiCCDS13557.1.
PIRiI38974.
RefSeqiNP_005277.2. NM_005286.2.
UniGeneiHs.248118.

3D structure databases

ProteinModelPortaliP48146.
SMRiP48146. Positions 9-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109093. 10 interactions.
IntActiP48146. 9 interactions.
STRINGi9606.ENSP00000358783.

Chemistry

GuidetoPHARMACOLOGYi304.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP48146.

Polymorphism and mutation databases

BioMutaiNPBWR2.

Proteomic databases

PaxDbiP48146.
PRIDEiP48146.

Protocols and materials databases

DNASUi2832.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369768; ENSP00000358783; ENSG00000125522.
ENST00000612646; ENSP00000484253; ENSG00000277339.
GeneIDi2832.
KEGGihsa:2832.
UCSCiuc011abt.2. human.

Organism-specific databases

CTDi2832.
GeneCardsiGC20M062737.
H-InvDBHIX0040594.
HGNCiHGNC:4530. NPBWR2.
MIMi600731. gene.
neXtProtiNX_P48146.
PharmGKBiPA28923.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG283184.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230485.
HOVERGENiHBG106919.
InParanoidiP48146.
KOiK08377.
OMAiPERFWFK.
OrthoDBiEOG7BKCVQ.
PhylomeDBiP48146.
TreeFamiTF315737.

Enzyme and pathway databases

ReactomeiREACT_14819. Peptide ligand-binding receptors.
REACT_19231. G alpha (i) signalling events.

Miscellaneous databases

GeneWikiiNeuropeptides_B/W_receptor_2.
GenomeRNAii2832.
NextBioi11177.
PROiP48146.
SOURCEiSearch...

Gene expression databases

BgeeiP48146.
CleanExiHS_NPBWR2.
GenevisibleiP48146. HS.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR009150. Neuropept_B/W_rcpt.
IPR027349. NPBW2.
[Graphical view]
PANTHERiPTHR24229:SF18. PTHR24229:SF18. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01855. NRPEPTIDEWR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The cloning and chromosomal mapping of two novel human opioid-somatostatin-like receptor genes, GPR7 and GPR8, expressed in discrete areas of the brain."
    O'Dowd B.F., Scheideler M.A., Nguyen T., Cheng R., Rasmussen J.S., Marchese A., Zastawny R., Heng H.H.Q., Tsui L.-C., Shi X., Asa S., Puy L., George S.R.
    Genomics 28:84-91(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ARG-206.
  2. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Identification of natural ligands for the orphan G protein-coupled receptors GPR7 and GPR8."
    Brezillon S., Lannoy V., Franssen J.-D., Le Poul E., Dupriez V., Lucchetti J., Detheux M., Parmentier M.
    J. Biol. Chem. 278:776-783(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INTERACTION WITH NEUROPEPTIDES B AND W.
  6. Cited for: INTERACTION WITH NEUROPEPTIDES B AND W.
  7. "Identification of a novel amino acid substitution (R206Q) in the second extracellular loop of the opioid-somatostatin-like receptor gene GPR8."
    Zeng J., Liu M., Grau O., Capron A., Bahr G.M.
    Hum. Mutat. 12:219-219(1998)
    Cited for: VARIANT ARG-206.
  8. Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-92.

Entry informationi

Entry nameiNPBW2_HUMAN
AccessioniPrimary (citable) accession number: P48146
Secondary accession number(s): Q6NWQ6, Q9H4K3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 13, 2007
Last modified: July 22, 2015
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.