Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P48146

- NPBW2_HUMAN

UniProt

P48146 - NPBW2_HUMAN

Protein

Neuropeptides B/W receptor type 2

Gene

NPBWR2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 2 (13 Nov 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals.

    GO - Molecular functioni

    1. neuropeptide receptor activity Source: InterPro
    2. opioid receptor activity Source: ProtInc
    3. protein binding Source: HGNC

    GO - Biological processi

    1. G-protein coupled receptor signaling pathway Source: HGNC
    2. opioid receptor signaling pathway Source: GOC

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_14819. Peptide ligand-binding receptors.
    REACT_19231. G alpha (i) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuropeptides B/W receptor type 2
    Alternative name(s):
    G-protein coupled receptor 8
    Gene namesi
    Name:NPBWR2
    Synonyms:GPR8
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 20

    Organism-specific databases

    HGNCiHGNC:4530. NPBWR2.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: HGNC
    2. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28923.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 333333Neuropeptides B/W receptor type 2PRO_0000069522Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi29 – 291N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi117 ↔ 197PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiP48146.
    PRIDEiP48146.

    PTM databases

    PhosphoSiteiP48146.

    Expressioni

    Tissue specificityi

    Detected at high levels in caudate nucleus, hyppocampus and amygdala; at moderate levels in the adult brain, thalamus, parietal cortex, pituitary gland, adrenal gland and lymph nodes.1 Publication

    Gene expression databases

    BgeeiP48146.
    CleanExiHS_NPBWR2.
    GenevestigatoriP48146.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000358783.

    Structurei

    3D structure databases

    ProteinModelPortaliP48146.
    SMRiP48146. Positions 9-333.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4545ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini70 – 8011CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini106 – 12015ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini141 – 16525CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini186 – 21126ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini234 – 25724CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini283 – 29210ExtracellularSequence Analysis
    Topological domaini308 – 33326CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei46 – 6924Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei81 – 10525Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei121 – 14020Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei166 – 18520Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei212 – 23322Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei258 – 28225Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei293 – 30715Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG283184.
    HOGENOMiHOG000230485.
    HOVERGENiHBG106919.
    InParanoidiP48146.
    KOiK08377.
    OMAiPERFWFK.
    OrthoDBiEOG7BKCVQ.
    PhylomeDBiP48146.
    TreeFamiTF315737.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR009150. Neuropept_B/W_rcpt.
    IPR027349. NPBW2.
    [Graphical view]
    PANTHERiPTHR24229:SF18. PTHR24229:SF18. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR01855. NRPEPTIDEWR.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48146-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQAAGHPEPL DSRGSFSLPT MGANVSQDNG TGHNATFSEP LPFLYVLLPA    50
    VYSGICAVGL TGNTAVILVI LRAPKMKTVT NVFILNLAVA DGLFTLVLPV 100
    NIAEHLLQYW PFGELLCKLV LAVDHYNIFS SIYFLAVMSV DRYLVVLATV 150
    RSRHMPWRTY RGAKVASLCV WLGVTVLVLP FFSFAGVYSN ELQVPSCGLS 200
    FPWPEQVWFK ASRVYTLVLG FVLPVCTICV LYTDLLRRLR AVRLRSGAKA 250
    LGKARRKVTV LVLVVLAVCL LCWTPFHLAS VVALTTDLPQ TPLVISMSYV 300
    ITSLSYANSC LNPFLYAFLD DNFRKNFRSI LRC 333
    Length:333
    Mass (Da):36,861
    Last modified:November 13, 2007 - v2
    Checksum:i8C416473FD80B0BA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti305 – 3051S → T in AAC50198. (PubMed:7590751)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti92 – 921G → R in a colorectal cancer sample; somatic mutation. 1 Publication
    VAR_035766
    Natural varianti206 – 2061Q → R.2 Publications
    Corresponds to variant rs4809401 [ dbSNP | Ensembl ].
    VAR_003579

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22492 Genomic DNA. Translation: AAC50198.1.
    AL121581 Genomic DNA. Translation: CAC17004.1.
    CH471077 Genomic DNA. Translation: EAW75161.1.
    BC067481 mRNA. Translation: AAH67481.1.
    BC067482 mRNA. Translation: AAH67482.1.
    CCDSiCCDS13557.1.
    PIRiI38974.
    RefSeqiNP_005277.2. NM_005286.2.
    UniGeneiHs.248118.

    Genome annotation databases

    EnsembliENST00000369768; ENSP00000358783; ENSG00000125522.
    GeneIDi2832.
    KEGGihsa:2832.
    UCSCiuc011abt.2. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22492 Genomic DNA. Translation: AAC50198.1 .
    AL121581 Genomic DNA. Translation: CAC17004.1 .
    CH471077 Genomic DNA. Translation: EAW75161.1 .
    BC067481 mRNA. Translation: AAH67481.1 .
    BC067482 mRNA. Translation: AAH67482.1 .
    CCDSi CCDS13557.1.
    PIRi I38974.
    RefSeqi NP_005277.2. NM_005286.2.
    UniGenei Hs.248118.

    3D structure databases

    ProteinModelPortali P48146.
    SMRi P48146. Positions 9-333.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000358783.

    Chemistry

    GuidetoPHARMACOLOGYi 304.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P48146.

    Proteomic databases

    PaxDbi P48146.
    PRIDEi P48146.

    Protocols and materials databases

    DNASUi 2832.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000369768 ; ENSP00000358783 ; ENSG00000125522 .
    GeneIDi 2832.
    KEGGi hsa:2832.
    UCSCi uc011abt.2. human.

    Organism-specific databases

    CTDi 2832.
    GeneCardsi GC20M062737.
    H-InvDB HIX0040594.
    HGNCi HGNC:4530. NPBWR2.
    MIMi 600731. gene.
    neXtProti NX_P48146.
    PharmGKBi PA28923.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG283184.
    HOGENOMi HOG000230485.
    HOVERGENi HBG106919.
    InParanoidi P48146.
    KOi K08377.
    OMAi PERFWFK.
    OrthoDBi EOG7BKCVQ.
    PhylomeDBi P48146.
    TreeFami TF315737.

    Enzyme and pathway databases

    Reactomei REACT_14819. Peptide ligand-binding receptors.
    REACT_19231. G alpha (i) signalling events.

    Miscellaneous databases

    GeneWikii Neuropeptides_B/W_receptor_2.
    GenomeRNAii 2832.
    NextBioi 11177.
    PROi P48146.
    SOURCEi Search...

    Gene expression databases

    Bgeei P48146.
    CleanExi HS_NPBWR2.
    Genevestigatori P48146.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR009150. Neuropept_B/W_rcpt.
    IPR027349. NPBW2.
    [Graphical view ]
    PANTHERi PTHR24229:SF18. PTHR24229:SF18. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR01855. NRPEPTIDEWR.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The cloning and chromosomal mapping of two novel human opioid-somatostatin-like receptor genes, GPR7 and GPR8, expressed in discrete areas of the brain."
      O'Dowd B.F., Scheideler M.A., Nguyen T., Cheng R., Rasmussen J.S., Marchese A., Zastawny R., Heng H.H.Q., Tsui L.-C., Shi X., Asa S., Puy L., George S.R.
      Genomics 28:84-91(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ARG-206.
    2. "The DNA sequence and comparative analysis of human chromosome 20."
      Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
      , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
      Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Identification of natural ligands for the orphan G protein-coupled receptors GPR7 and GPR8."
      Brezillon S., Lannoy V., Franssen J.-D., Le Poul E., Dupriez V., Lucchetti J., Detheux M., Parmentier M.
      J. Biol. Chem. 278:776-783(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INTERACTION WITH NEUROPEPTIDES B AND W.
    6. Cited for: INTERACTION WITH NEUROPEPTIDES B AND W.
    7. "Identification of a novel amino acid substitution (R206Q) in the second extracellular loop of the opioid-somatostatin-like receptor gene GPR8."
      Zeng J., Liu M., Grau O., Capron A., Bahr G.M.
      Hum. Mutat. 12:219-219(1998)
      Cited for: VARIANT ARG-206.
    8. Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-92.

    Entry informationi

    Entry nameiNPBW2_HUMAN
    AccessioniPrimary (citable) accession number: P48146
    Secondary accession number(s): Q6NWQ6, Q9H4K3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 139 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome 20
      Human chromosome 20: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3