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P48146 (NPBW2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuropeptides B/W receptor type 2
Alternative name(s):
G-protein coupled receptor 8
Gene names
Name:NPBWR2
Synonyms:GPR8
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Detected at high levels in caudate nucleus, hyppocampus and amygdala; at moderate levels in the adult brain, thalamus, parietal cortex, pituitary gland, adrenal gland and lymph nodes. Ref.5

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 333333Neuropeptides B/W receptor type 2
PRO_0000069522

Regions

Topological domain1 – 4545Extracellular Potential
Transmembrane46 – 6924Helical; Name=1; Potential
Topological domain70 – 8011Cytoplasmic Potential
Transmembrane81 – 10525Helical; Name=2; Potential
Topological domain106 – 12015Extracellular Potential
Transmembrane121 – 14020Helical; Name=3; Potential
Topological domain141 – 16525Cytoplasmic Potential
Transmembrane166 – 18520Helical; Name=4; Potential
Topological domain186 – 21126Extracellular Potential
Transmembrane212 – 23322Helical; Name=5; Potential
Topological domain234 – 25724Cytoplasmic Potential
Transmembrane258 – 28225Helical; Name=6; Potential
Topological domain283 – 29210Extracellular Potential
Transmembrane293 – 30715Helical; Name=7; Potential
Topological domain308 – 33326Cytoplasmic Potential

Amino acid modifications

Glycosylation241N-linked (GlcNAc...) Potential
Glycosylation291N-linked (GlcNAc...) Potential
Glycosylation341N-linked (GlcNAc...) Potential
Disulfide bond117 ↔ 197 By similarity

Natural variations

Natural variant921G → R in a colorectal cancer sample; somatic mutation. Ref.8
VAR_035766
Natural variant2061Q → R. Ref.1 Ref.7
Corresponds to variant rs4809401 [ dbSNP | Ensembl ].
VAR_003579

Experimental info

Sequence conflict3051S → T in AAC50198. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P48146 [UniParc].

Last modified November 13, 2007. Version 2.
Checksum: 8C416473FD80B0BA

FASTA33336,861
        10         20         30         40         50         60 
MQAAGHPEPL DSRGSFSLPT MGANVSQDNG TGHNATFSEP LPFLYVLLPA VYSGICAVGL 

        70         80         90        100        110        120 
TGNTAVILVI LRAPKMKTVT NVFILNLAVA DGLFTLVLPV NIAEHLLQYW PFGELLCKLV 

       130        140        150        160        170        180 
LAVDHYNIFS SIYFLAVMSV DRYLVVLATV RSRHMPWRTY RGAKVASLCV WLGVTVLVLP 

       190        200        210        220        230        240 
FFSFAGVYSN ELQVPSCGLS FPWPEQVWFK ASRVYTLVLG FVLPVCTICV LYTDLLRRLR 

       250        260        270        280        290        300 
AVRLRSGAKA LGKARRKVTV LVLVVLAVCL LCWTPFHLAS VVALTTDLPQ TPLVISMSYV 

       310        320        330 
ITSLSYANSC LNPFLYAFLD DNFRKNFRSI LRC 

« Hide

References

« Hide 'large scale' references
[1]"The cloning and chromosomal mapping of two novel human opioid-somatostatin-like receptor genes, GPR7 and GPR8, expressed in discrete areas of the brain."
O'Dowd B.F., Scheideler M.A., Nguyen T., Cheng R., Rasmussen J.S., Marchese A., Zastawny R., Heng H.H.Q., Tsui L.-C., Shi X., Asa S., Puy L., George S.R.
Genomics 28:84-91(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ARG-206.
[2]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Identification of natural ligands for the orphan G protein-coupled receptors GPR7 and GPR8."
Brezillon S., Lannoy V., Franssen J.-D., Le Poul E., Dupriez V., Lucchetti J., Detheux M., Parmentier M.
J. Biol. Chem. 278:776-783(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INTERACTION WITH NEUROPEPTIDES B AND W.
[6]"Characterization of a family of endogenous neuropeptide ligands for the G protein-coupled receptors GPR7 and GPR8."
Tanaka H., Yoshida T., Miyamoto N., Motoike T., Kurosu H., Shibata K., Yamanaka A., Williams S.C., Richardson J.A., Tsujino N., Garry M.G., Lerner M.R., King D.S., O'Dowd B.F., Sakurai T., Yanagisawa M.
Proc. Natl. Acad. Sci. U.S.A. 100:6251-6256(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NEUROPEPTIDES B AND W.
[7]"Identification of a novel amino acid substitution (R206Q) in the second extracellular loop of the opioid-somatostatin-like receptor gene GPR8."
Zeng J., Liu M., Grau O., Capron A., Bahr G.M.
Hum. Mutat. 12:219-219(1998)
Cited for: VARIANT ARG-206.
[8]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-92.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U22492 Genomic DNA. Translation: AAC50198.1.
AL121581 Genomic DNA. Translation: CAC17004.1.
CH471077 Genomic DNA. Translation: EAW75161.1.
BC067481 mRNA. Translation: AAH67481.1.
BC067482 mRNA. Translation: AAH67482.1.
PIRI38974.
RefSeqNP_005277.2. NM_005286.2.
UniGeneHs.248118.

3D structure databases

ProteinModelPortalP48146.
SMRP48146. Positions 9-333.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000358783.

Chemistry

GuidetoPHARMACOLOGY304.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP48146.

Proteomic databases

PaxDbP48146.
PRIDEP48146.

Protocols and materials databases

DNASU2832.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000369768; ENSP00000358783; ENSG00000125522.
GeneID2832.
KEGGhsa:2832.
UCSCuc011abt.2. human.

Organism-specific databases

CTD2832.
GeneCardsGC20M062737.
H-InvDBHIX0040594.
HGNCHGNC:4530. NPBWR2.
MIM600731. gene.
neXtProtNX_P48146.
PharmGKBPA28923.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG283184.
HOGENOMHOG000230485.
HOVERGENHBG106919.
InParanoidP48146.
KOK08377.
OMAELQVPSC.
OrthoDBEOG7BKCVQ.
PhylomeDBP48146.
TreeFamTF315737.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

BgeeP48146.
CleanExHS_NPBWR2.
GenevestigatorP48146.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR009150. Neuropept_B/W_rcpt.
IPR027349. NPBW2.
[Graphical view]
PANTHERPTHR24229:SF18. PTHR24229:SF18. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR01855. NRPEPTIDEWR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiNeuropeptides_B/W_receptor_2.
GenomeRNAi2832.
NextBio11177.
PROP48146.
SOURCESearch...

Entry information

Entry nameNPBW2_HUMAN
AccessionPrimary (citable) accession number: P48146
Secondary accession number(s): Q6NWQ6, Q9H4K3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 13, 2007
Last modified: April 16, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries