ID   NPBW1_HUMAN             Reviewed;         328 AA.
AC   P48145; Q6NTC7;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 2.
DT   27-MAR-2024, entry version 184.
DE   RecName: Full=Neuropeptides B/W receptor type 1;
DE   AltName: Full=G-protein coupled receptor 7;
GN   Name=NPBWR1; Synonyms=GPR7;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7590751; DOI=10.1006/geno.1995.1109;
RA   O'Dowd B.F., Scheideler M.A., Nguyen T., Cheng R., Rasmussen J.S.,
RA   Marchese A., Zastawny R., Heng H.H.Q., Tsui L.-C., Shi X., Asa S., Puy L.,
RA   George S.R.;
RT   "The cloning and chromosomal mapping of two novel human opioid-
RT   somatostatin-like receptor genes, GPR7 and GPR8, expressed in discrete
RT   areas of the brain.";
RL   Genomics 28:84-91(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   TISSUE SPECIFICITY, AND INTERACTION WITH NEUROPEPTIDES B AND W.
RX   PubMed=12401809; DOI=10.1074/jbc.m206396200;
RA   Brezillon S., Lannoy V., Franssen J.-D., Le Poul E., Dupriez V.,
RA   Lucchetti J., Detheux M., Parmentier M.;
RT   "Identification of natural ligands for the orphan G protein-coupled
RT   receptors GPR7 and GPR8.";
RL   J. Biol. Chem. 278:776-783(2003).
RN   [5]
RP   INTERACTION WITH NEUROPEPTIDES B AND W.
RX   PubMed=12719537; DOI=10.1073/pnas.0837789100;
RA   Tanaka H., Yoshida T., Miyamoto N., Motoike T., Kurosu H., Shibata K.,
RA   Yamanaka A., Williams S.C., Richardson J.A., Tsujino N., Garry M.G.,
RA   Lerner M.R., King D.S., O'Dowd B.F., Sakurai T., Yanagisawa M.;
RT   "Characterization of a family of endogenous neuropeptide ligands for the G
RT   protein-coupled receptors GPR7 and GPR8.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:6251-6256(2003).
RN   [6]
RP   VARIANT [LARGE SCALE ANALYSIS] GLN-19.
RX   PubMed=16959974; DOI=10.1126/science.1133427;
RA   Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA   Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA   Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA   Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA   Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA   Velculescu V.E.;
RT   "The consensus coding sequences of human breast and colorectal cancers.";
RL   Science 314:268-274(2006).
CC   -!- FUNCTION: Interacts specifically with a number of opioid ligands.
CC       Receptor for neuropeptides B and W, which may be involved in
CC       neuroendocrine system regulation, food intake and the organization of
CC       other signals. Has a higher affinity for neuropeptide B.
CC   -!- INTERACTION:
CC       P48145; Q9UHD9: UBQLN2; NbExp=3; IntAct=EBI-13061492, EBI-947187;
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Found in cerebellum and frontal cortex. Detected at
CC       high levels in hippocampus, amygdala and trachea; at moderate levels in
CC       fetal brain, pituitary gland and prostate. Not in caudate, accumbens,
CC       kidney or liver. Also detected at high levels in lung carcinoma.
CC       {ECO:0000269|PubMed:12401809}.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR   EMBL; U22491; AAC50197.1; -; Genomic_DNA.
DR   EMBL; CH471068; EAW86722.1; -; Genomic_DNA.
DR   EMBL; BC069117; AAH69117.1; -; mRNA.
DR   EMBL; BC107101; AAI07102.1; -; mRNA.
DR   CCDS; CCDS6151.1; -.
DR   PIR; I38973; I38973.
DR   RefSeq; NP_005276.2; NM_005285.3.
DR   AlphaFoldDB; P48145; -.
DR   SMR; P48145; -.
DR   BioGRID; 109092; 3.
DR   IntAct; P48145; 1.
DR   STRING; 9606.ENSP00000330284; -.
DR   BindingDB; P48145; -.
DR   ChEMBL; CHEMBL1293293; -.
DR   GuidetoPHARMACOLOGY; 303; -.
DR   GlyCosmos; P48145; 4 sites, 1 glycan.
DR   GlyGen; P48145; 4 sites, 1 O-linked glycan (1 site).
DR   iPTMnet; P48145; -.
DR   PhosphoSitePlus; P48145; -.
DR   BioMuta; NPBWR1; -.
DR   DMDM; 215274092; -.
DR   PaxDb; 9606-ENSP00000330284; -.
DR   PeptideAtlas; P48145; -.
DR   Antibodypedia; 82634; 236 antibodies from 30 providers.
DR   DNASU; 2831; -.
DR   Ensembl; ENST00000674939.1; ENSP00000501711.1; ENSG00000288611.1.
DR   GeneID; 2831; -.
DR   KEGG; hsa:2831; -.
DR   MANE-Select; ENST00000674939.1; ENSP00000501711.1; NM_005285.5; NP_005276.2.
DR   UCSC; uc011ldu.3; human.
DR   AGR; HGNC:4522; -.
DR   CTD; 2831; -.
DR   DisGeNET; 2831; -.
DR   GeneCards; NPBWR1; -.
DR   HGNC; HGNC:4522; NPBWR1.
DR   HPA; ENSG00000288611; Tissue enhanced (brain, liver).
DR   MIM; 600730; gene.
DR   neXtProt; NX_P48145; -.
DR   PharmGKB; PA28912; -.
DR   VEuPathDB; HostDB:ENSG00000288611; -.
DR   eggNOG; KOG3656; Eukaryota.
DR   GeneTree; ENSGT00940000161936; -.
DR   HOGENOM; CLU_009579_8_1_1; -.
DR   InParanoid; P48145; -.
DR   OMA; WKVSRLY; -.
DR   OrthoDB; 5393360at2759; -.
DR   PhylomeDB; P48145; -.
DR   TreeFam; TF315737; -.
DR   PathwayCommons; P48145; -.
DR   Reactome; R-HSA-375276; Peptide ligand-binding receptors.
DR   Reactome; R-HSA-418594; G alpha (i) signalling events.
DR   SignaLink; P48145; -.
DR   SIGNOR; P48145; -.
DR   BioGRID-ORCS; 2831; 15 hits in 1134 CRISPR screens.
DR   GeneWiki; Neuropeptides_B/W_receptor_1; -.
DR   GenomeRNAi; 2831; -.
DR   Pharos; P48145; Tchem.
DR   PRO; PR:P48145; -.
DR   Proteomes; UP000005640; Chromosome 8.
DR   RNAct; P48145; Protein.
DR   Bgee; ENSG00000288611; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 49 other cell types or tissues.
DR   ExpressionAtlas; P48145; differential.
DR   GO; GO:0016020; C:membrane; IC:HGNC-UCL.
DR   GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR   GO; GO:0045202; C:synapse; IEA:GOC.
DR   GO; GO:0004985; F:G protein-coupled opioid receptor activity; TAS:ProtInc.
DR   GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR   GO; GO:0042923; F:neuropeptide binding; IBA:GO_Central.
DR   GO; GO:0008188; F:neuropeptide receptor activity; IEA:InterPro.
DR   GO; GO:0007268; P:chemical synaptic transmission; TAS:ProtInc.
DR   GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:HGNC-UCL.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IBA:GO_Central.
DR   GO; GO:0019222; P:regulation of metabolic process; IEA:Ensembl.
DR   CDD; cd15087; 7tmA_NPBWR; 1.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR000276; GPCR_Rhodpsn.
DR   InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR   InterPro; IPR009150; Neuropept_B/W_rcpt.
DR   PANTHER; PTHR24229:SF47; NEUROPEPTIDES B_W RECEPTOR TYPE 1; 1.
DR   PANTHER; PTHR24229; NEUROPEPTIDES RECEPTOR; 1.
DR   Pfam; PF00001; 7tm_1; 1.
DR   PRINTS; PR00237; GPCRRHODOPSN.
DR   PRINTS; PR01855; NRPEPTIDEWR.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR   PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR   PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR   Genevisible; P48145; HS.
PE   1: Evidence at protein level;
KW   Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW   Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..328
FT                   /note="Neuropeptides B/W receptor type 1"
FT                   /id="PRO_0000069518"
FT   TOPO_DOM        1..37
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        38..61
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        62..72
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        73..97
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        98..112
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        113..132
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        133..157
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        158..177
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        178..202
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        203..224
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        225..248
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        249..273
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        274..283
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        284..298
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        299..328
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        3
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        13
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        25
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        109..188
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT   VARIANT         19
FT                   /note="P -> Q (in a breast cancer sample; somatic mutation;
FT                   dbSNP:rs772418985)"
FT                   /evidence="ECO:0000269|PubMed:16959974"
FT                   /id="VAR_035765"
FT   VARIANT         135
FT                   /note="Y -> F (in dbSNP:rs33977775)"
FT                   /id="VAR_047788"
FT   VARIANT         319
FT                   /note="R -> C (in dbSNP:rs36068168)"
FT                   /id="VAR_047789"
FT   CONFLICT        296
FT                   /note="S -> T (in Ref. 1; AAC50197)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   328 AA;  36103 MW;  7A90F85AD64A3980 CRC64;
     MDNASFSEPW PANASGPDPA LSCSNASTLA PLPAPLAVAV PVVYAVICAV GLAGNSAVLY
     VLLRAPRMKT VTNLFILNLA IADELFTLVL PINIADFLLR QWPFGELMCK LIVAIDQYNT
     FSSLYFLTVM SADRYLVVLA TAESRRVAGR TYSAARAVSL AVWGIVTLVV LPFAVFARLD
     DEQGRRQCVL VFPQPEAFWW RASRLYTLVL GFAIPVSTIC VLYTTLLCRL HAMRLDSHAK
     ALERAKKRVT FLVVAILAVC LLCWTPYHLS TVVALTTDLP QTPLVIAISY FITSLSYANS
     CLNPFLYAFL DASFRRNLRQ LITCRAAA
//