P48145 (NPBW1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuropeptides B/W receptor type 1

Alternative name(s):
G-protein coupled receptor 7

Gene names

Name:	NPBWR1
Synonyms:	GPR7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals. Has a higher affinity for neuropeptide B.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Found in cerebellum and frontal cortex. Detected at high levels in hippocampus, amygdala and trachea; at moderate levels in fetal brain, pituitary gland and prostate. Not in caudate, accumbens, kidney or liver. Also detected at high levels in lung carcinoma. Ref.4
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	neuropeptide signaling pathway Inferred from electronic annotation. Source: GOC regulation of metabolic process Inferred from electronic annotation. Source: Compara synaptic transmission Traceable author statement Ref.1. Source: ProtInc
Cellular_component	integral to membrane Inferred by curator Ref.4. Source: HGNC plasma membrane Traceable author statement. Source: Reactome
Molecular_function	neuropeptide receptor activity Inferred from electronic annotation. Source: InterPro opioid receptor activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Neuropeptides B/W receptor type 1		PRO_0000069518
Regions
	Topological domain	1 – 37	37	Extracellular Potential
	Transmembrane	38 – 61	24	Helical; Name=1; Potential
	Topological domain	62 – 72	11	Cytoplasmic Potential
	Transmembrane	73 – 97	25	Helical; Name=2; Potential
	Topological domain	98 – 112	15	Extracellular Potential
	Transmembrane	113 – 132	20	Helical; Name=3; Potential
	Topological domain	133 – 157	25	Cytoplasmic Potential
	Transmembrane	158 – 177	20	Helical; Name=4; Potential
	Topological domain	178 – 202	25	Extracellular Potential
	Transmembrane	203 – 224	22	Helical; Name=5; Potential
	Topological domain	225 – 248	24	Cytoplasmic Potential
	Transmembrane	249 – 273	25	Helical; Name=6; Potential
	Topological domain	274 – 283	10	Extracellular Potential
	Transmembrane	284 – 298	15	Helical; Name=7; Potential
	Topological domain	299 – 328	30	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	3	1	N-linked (GlcNAc...) Potential
	Glycosylation	13	1	N-linked (GlcNAc...) Potential
	Glycosylation	25	1	N-linked (GlcNAc...) Potential
	Disulfide bond	109 ↔ 188		By similarity
Natural variations
	Natural variant	19	1	P → Q in a breast cancer sample; somatic mutation. Ref.6		VAR_035765
	Natural variant	135	1	Y → F. Corresponds to variant rs33977775 [ dbSNP | Ensembl ].		VAR_047788
	Natural variant	319	1	R → C. Corresponds to variant rs36068168 [ dbSNP | Ensembl ].		VAR_047789
Experimental info
	Sequence conflict	296	1	S → T in AAC50197. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48145 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: 7A90F85AD64A3980	FASTA	328	36,103
	10 20 30 40 50 60 MDNASFSEPW PANASGPDPA LSCSNASTLA PLPAPLAVAV PVVYAVICAV GLAGNSAVLY 70 80 90 100 110 120 VLLRAPRMKT VTNLFILNLA IADELFTLVL PINIADFLLR QWPFGELMCK LIVAIDQYNT 130 140 150 160 170 180 FSSLYFLTVM SADRYLVVLA TAESRRVAGR TYSAARAVSL AVWGIVTLVV LPFAVFARLD 190 200 210 220 230 240 DEQGRRQCVL VFPQPEAFWW RASRLYTLVL GFAIPVSTIC VLYTTLLCRL HAMRLDSHAK 250 260 270 280 290 300 ALERAKKRVT FLVVAILAVC LLCWTPYHLS TVVALTTDLP QTPLVIAISY FITSLSYANS 310 320 CLNPFLYAFL DASFRRNLRQ LITCRAAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cloning and chromosomal mapping of two novel human opioid-somatostatin-like receptor genes, GPR7 and GPR8, expressed in discrete areas of the brain." O'Dowd B.F., Scheideler M.A., Nguyen T., Cheng R., Rasmussen J.S., Marchese A., Zastawny R., Heng H.H.Q., Tsui L.-C., Shi X., Asa S., Puy L., George S.R. Genomics 28:84-91(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Identification of natural ligands for the orphan G protein-coupled receptors GPR7 and GPR8." Brezillon S., Lannoy V., Franssen J.-D., Le Poul E., Dupriez V., Lucchetti J., Detheux M., Parmentier M. J. Biol. Chem. 278:776-783(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INTERACTION WITH NEUROPEPTIDES B AND W.
[5]	"Characterization of a family of endogenous neuropeptide ligands for the G protein-coupled receptors GPR7 and GPR8." Tanaka H., Yoshida T., Miyamoto N., Motoike T., Kurosu H., Shibata K., Yamanaka A., Williams S.C., Richardson J.A., Tsujino N., Garry M.G., Lerner M.R., King D.S., O'Dowd B.F., Sakurai T., Yanagisawa M. Proc. Natl. Acad. Sci. U.S.A. 100:6251-6256(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NEUROPEPTIDES B AND W.
[6]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] GLN-19.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U22491 Genomic DNA. Translation: AAC50197.1. CH471068 Genomic DNA. Translation: EAW86722.1. BC069117 mRNA. Translation: AAH69117.1. BC107101 mRNA. Translation: AAI07102.1.
IPI	IPI00008160.
PIR	I38973.
RefSeq	NP_005276.2. NM_005285.3.
UniGene	Hs.248117.
3D structure databases
ProteinModelPortal	P48145.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000330284.
Protein family/group databases
GPCRDB	Search...
Polymorphism databases
DMDM	215274092.
Proteomic databases
PRIDE	P48145.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000331251; ENSP00000330284; ENSG00000183729.
GeneID	2831.
KEGG	hsa:2831.
UCSC	uc011ldu.2. human.
Organism-specific databases
CTD	2831.
GeneCards	GC08P053850.
H-InvDB	HIX0034348.
HGNC	HGNC:4522. NPBWR1.
MIM	600730. gene.
neXtProt	NX_P48145.
PharmGKB	PA28912.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG283184.
HOGENOM	HOG000230485.
HOVERGEN	HBG106919.
InParanoid	P48145.
KO	K05268.
OMA	CRLRAIR.
OrthoDB	EOG49GKH1.
PhylomeDB	P48145.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	P48145.
Bgee	P48145.
CleanEx	HS_NPBWR1.
Genevestigator	P48145.
GermOnline	ENSG00000183729. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR009150. Neuropept_B/W_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01855. NRPEPTIDEWR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P48145.
ChEMBL	CHEMBL1293293.
GenomeRNAi	2831.
NextBio	11173.
SOURCE	Search...

Entry information

Entry name

NPBW1_HUMAN

Accession

Primary (citable) accession number: P48145
Secondary accession number(s): Q6NTC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.