P48110 (CHLL_CYAPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein
Short name=DPOR subunit L
Short name=LI-POR subunit L
EC=1.18.-.-

Gene names

Name:	chlL
Synonyms:	frxC

Encoded on

Plastid; Cyanelle

Organism

Cyanophora paradoxa

Taxonomic identifier

2762 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Glaucocystophyceae › Cyanophoraceae › Cyanophora

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). This reaction is light-independent By similarity. HAMAP-Rule MF_00355
Cofactor	Binds 1 4Fe-4S cluster per dimer By similarity. HAMAP-Rule MF_00355
Pathway	Porphyrin-containing compound metabolism; chlorophyll biosynthesis (light-independent).
Subunit structure	Protochlorophyllide reductase is thought to be composed of three subunits; chlL, chlN and chlB. Homodimer of chlL subunit By similarity.
Subcellular location	Plastid › cyanelle HAMAP-Rule MF_00355.
Sequence similarities	Belongs to the NifH/BchL/ChlL family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Cyanelle Plastid
Ligand	4Fe-4S ATP-binding Iron Iron-sulfur Metal-binding Nucleotide-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	bacteriochlorophyll biosynthetic process Inferred from electronic annotation. Source: HAMAP light-independent chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway photosynthesis, dark reaction Inferred from electronic annotation. Source: InterPro
Cellular_component	cyanelle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on iron-sulfur proteins as donors Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein HAMAP-Rule MF_00355		PRO_0000139556
Regions
	Nucleotide binding	7 – 14	8	ATP Potential
Sites
	Metal binding	95	1	Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity
	Metal binding	129	1	Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48110 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: BC5072D21AE28684	FASTA	282	30,847
	10 20 30 40 50 60 MKLAVYGKGG IGKSTTSCNI SVALAKRGKK VLQIGCDPKH DSTFTLTGHL IPTIIDTLQE 70 80 90 100 110 120 KDFHYEDIWP EDVIYKGYAG VDCVEAGGPP AGAGCGGYVV GETVKLLKEL NAFDEYDIIL 130 140 150 160 170 180 FDVLGDVVCG GFASPLNYAD YCLIVTDNGF DALFAANRIA ASVREKARTH QLRLAGLIGN 190 200 210 220 230 240 RTTKSDLIEK YTENVPIPIL QLLPLIEDIR ISRVKGKTLF EMSESNPELS PICDYYLNIA 250 260 270 280 DQILAKPEGI IPNEVLDRDL FTLLSDFYLN MDSSESSNLT LV

References

[1]

"Nucleotide sequence of the cyanelle DNA from Cyanophora paradoxa." Stirewalt V.L., Michalowski C.B., Loeffelhardt W., Bohnert H.J., Bryant D.A. Plant Mol. Biol. Rep. 13:327-332(1995) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UTEX LB 555 / Pringsheim.

[2]

"The complete sequence of the cyanelle genome of Cyanophora paradoxa: the genetic complexity of a primitive plastid." Loeffelhardt W., Stirewalt V.L., Michalowski C.B., Annarella M., Farley J.Y., Schluchter W.M., Chung S., Newmann-Spallart C., Steiner J.M., Jakowitsch J., Bohnert H.J., Bryant D.A. (In) Schenk H.E.A., Herrmann R., Jeon K.W., Mueller N.E., Schwemmler W. (eds.); Eukaryotism and symbiosis, pp.40-48, Springer-Verlag, Heidelberg (1997) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UTEX LB 555 / Pringsheim.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U30821 Genomic DNA. Translation: AAA81316.1.
PIR	T06973.
RefSeq	NP_043285.1. NC_001675.1.
3D structure databases
ProteinModelPortal	P48110.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	801629.
Phylogenomic databases
ProtClustDB	CHL00072.
Enzyme and pathway databases
UniPathway	UPA00670.
Family and domain databases
HAMAP	MF_00355. ChlL_BchL.
InterPro	IPR000392. Nitogenase_NifH/Reductase_ChlL. IPR027417. P-loop_NTPase. IPR005971. Protochlorophyllide_ATP-bd. [Graphical view]
Pfam	PF00142. Fer4_NifH. 1 hit. [Graphical view]
PIRSF	PIRSF000363. Nitrogenase_iron. 1 hit.
PRINTS	PR00091. NITROGNASEII.
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR01281. DPOR_bchL. 1 hit.
PROSITE	PS00746. NIFH_FRXC_1. 1 hit. PS00692. NIFH_FRXC_2. 1 hit. PS51026. NIFH_FRXC_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHLL_CYAPA

Accession

Primary (citable) accession number: P48110

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)