Skip Header

Contribute Send feedback
Read comments (?) or add your own

P48076 (PA2GC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Group IIC secretory phospholipase A2
Short name=GIIC sPLA2
Short name=sPLA2-IIC
EC=3.1.1.4
Alternative name(s):
14 kDa phospholipase A2
PLA2-8
Phosphatidylcholine 2-acylhydrolase 2C
Gene names
Name:Pla2g2c
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length150 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Testis PA2 may be important in the production of prostaglandins, by the release of arachidonic acid, which in turn are necessary for the contractions of the seminiferous tubules and the testicular capsule; they also seem to decrease sperm transit time through the male reproductive tract.

Catalytic activity

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subcellular location

Secreted Potential.

Tissue specificity

Testis specific.

Developmental stage

Expressed mainly in pachytene and secondary spermatocytes and round spermatids and predominates in stage VI-VII tubules.

Sequence similarities

Belongs to the phospholipase A2 family.

Ontologies

Keywords
   Biological processLipid degradation
Lipid metabolism
   Cellular componentSecreted
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionHydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processphospholipid catabolic process

Inferred from sequence orthology Ref.2. Source: MGI

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

phospholipase A2 activity

Inferred from sequence orthology Ref.2. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 150130Group IIC secretory phospholipase A2
PRO_0000022753

Sites

Active site671 By similarity
Active site1151 By similarity
Metal binding471Calcium; via carbonyl oxygen By similarity
Metal binding491Calcium; via carbonyl oxygen By similarity
Metal binding511Calcium; via carbonyl oxygen By similarity
Metal binding681Calcium By similarity

Amino acid modifications

Glycosylation921N-linked (GlcNAc...) Potential
Disulfide bond46 ↔ 143 By similarity
Disulfide bond48 ↔ 64 By similarity
Disulfide bond63 ↔ 121 By similarity
Disulfide bond69 ↔ 150 By similarity
Disulfide bond70 ↔ 114 By similarity
Disulfide bond79 ↔ 107 By similarity
Disulfide bond97 ↔ 112 By similarity
Disulfide bond99 ↔ 105 Potential

Experimental info

Sequence conflict281M → W Ref.2
Sequence conflict771Y → W Ref.2

Sequences

Sequence LengthMass (Da)Tools
P48076 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: C2D5EF5E7B19972F

FASTA15016,983
        10         20         30         40         50         60 
MKGIAIFLVF IFYWTTSTLS SFWQFQRMVK HVTGRSAFFS YYGYGCYCGL GGKGLPVDAT 

        70         80         90        100        110        120 
DRCCWAHDCC YHKLKEYGCQ PILNAYQFTI VNGTVTCGCT VASSCPCGQK ACECDKQSVY 

       130        140        150 
CFKENLATYE KAFKQLFPTR PQCGRDKLQC

« Hide

References

[1]"Localization of group IIc low molecular weight phospholipase A2 mRNA to meiotic cells in the mouse."
Chen J., Shao C., Lazar V., Srivastava C.H., Lee W., Tischfield J.A.
J. Cell. Biochem. 64:369-375(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning and characterization of novel rat and mouse low molecular weight Ca(2+)-dependent phospholipase A2s containing 16 cysteines."
Chen J., Engle S.J., Seilhamer J.J., Tischfield J.A.
J. Biol. Chem. 269:23018-23024(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-150.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U18119 mRNA. Translation: AAC52936.1.
IPIIPI00138211.
PIRA54762.
RefSeqNP_032894.2. NM_008868.3.
UniGeneMm.5189.

3D structure databases

ProteinModelPortalP48076.
SMRP48076. Positions 24-150.
ModBaseSearch...

PTM databases

PhosphoSiteP48076.

Proteomic databases

PRIDEP48076.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID18781.
KEGGmmu:18781.

Organism-specific databases

CTD391013.
MGIMGI:106638. Pla2g2c.

Phylogenomic databases

eggNOGNOG139737.
HOGENOMHOG000231749.
HOVERGENHBG008137.
InParanoidP48076.
KOK01047.
OrthoDBEOG47SSG3.

Gene expression databases

CleanExMM_PLA2G2C.
GenevestigatorP48076.
GermOnlineENSMUSG00000028750. Mus musculus.

Family and domain databases

Gene3D1.20.90.10. 1 hit.
InterProIPR001211. PLipase_A2.
IPR013090. PLipase_A2_AS.
IPR016090. PLipase_A2_dom.
[Graphical view]
PANTHERPTHR11716. PTHR11716. 1 hit.
PfamPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSPR00389. PHPHLIPASEA2.
SMARTSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMSSF48619. PhospholipaseA2. 1 hit.
PROSITEPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio295036.
SOURCESearch...

Entry information

Entry namePA2GC_MOUSE
AccessionPrimary (citable) accession number: P48076
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 1, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents