P48067 (SC6A9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium- and chloride-dependent glycine transporter 1
Short name=GlyT-1
Short name=GlyT1

Alternative name(s):
Solute carrier family 6 member 9

Gene names

Name:

SLC6A9

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	706 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May play a role in regulation of glycine levels in NMDA receptor-mediated neurotransmission.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Isoform GlyT-1A and isoform GlyT-1B can be found in brain, kidney, pancreas, lung, placenta and liver but isoform GlyT-1C is only found in brain.
Sequence similarities	Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A9 subfamily. [View classification]
Sequence caution	The sequence AAB30784.1 differs from that shown. Reason: Frameshift at position 8. The sequence AAB30785.1 differs from that shown. Reason: Frameshift at position 8. The sequence CAI19428.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAI19429.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Amino-acid transport Neurotransmitter transport Symport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	glycine:sodium symporter activity Traceable author statement Ref.1. Source: ProtInc neurotransmitter:sodium symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform GlyT-1C (identifier: P48067-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform GlyT-1A (identifier: P48067-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-83: MSGGDTRAAI...MAQHSLAMAQ → MVGKGAKGML

Isoform GlyT-1B (identifier: P48067-3)

The sequence of this isoform differs from the canonical sequence as follows:
     29-82: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 706	706	Sodium- and chloride-dependent glycine transporter 1		PRO_0000214780
Regions
	Topological domain	1 – 108	108	Cytoplasmic Potential
	Transmembrane	109 – 129	21	Helical; Name=1; Potential
	Transmembrane	136 – 156	21	Helical; Name=2; Potential
	Transmembrane	188 – 208	21	Helical; Name=3; Potential
	Topological domain	209 – 285	77	Extracellular Potential
	Transmembrane	286 – 306	21	Helical; Name=4; Potential
	Transmembrane	315 – 335	21	Helical; Name=5; Potential
	Transmembrane	360 – 380	21	Helical; Name=6; Potential
	Transmembrane	407 – 427	21	Helical; Name=7; Potential
	Transmembrane	450 – 470	21	Helical; Name=8; Potential
	Transmembrane	506 – 526	21	Helical; Name=9; Potential
	Transmembrane	530 – 550	21	Helical; Name=10; Potential
	Transmembrane	570 – 590	21	Helical; Name=11; Potential
	Transmembrane	610 – 630	21	Helical; Name=12; Potential
	Topological domain	631 – 706	76	Cytoplasmic Potential
Natural variations
	Alternative sequence	1 – 83	83	MSGGD…LAMAQ → MVGKGAKGML in isoform GlyT-1A.		VSP_006270
	Alternative sequence	29 – 82	54	Missing in isoform GlyT-1B.		VSP_006271
Experimental info
	Sequence conflict	373	1	G → A in AAB30784. Ref.1
	Sequence conflict	373	1	G → A in AAB30785. Ref.1
	Sequence conflict	682	1	P → S in AAB30784. Ref.1
	Sequence conflict	682	1	P → S in AAB30785. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform GlyT-1C [UniParc]. Last modified August 10, 2010. Version 3. Checksum: 654B208568F843F4	FASTA	706	78,260
	10 20 30 40 50 60 MSGGDTRAAI ARPRMAAAHG PVAPSSPEQV TLLPVQRSFF LPPFSGATPS TSLAESVLKV 70 80 90 100 110 120 WHGAYNSGLL PQLMAQHSLA MAQNGAVPSE ATKRDQNLKR GNWGNQIEFV LTSVGYAVGL 130 140 150 160 170 180 GNVWRFPYLC YRNGGGAFMF PYFIMLIFCG IPLFFMELSF GQFASQGCLG VWRISPMFKG 190 200 210 220 230 240 VGYGMMVVST YIGIYYNVVI CIAFYYFFSS MTHVLPWAYC NNPWNTHDCA GVLDASNLTN 250 260 270 280 290 300 GSRPAALPSN LSHLLNHSLQ RTSPSEEYWR LYVLKLSDDI GNFGEVRLPL LGCLGVSWLV 310 320 330 340 350 360 VFLCLIRGVK SSGKVVYFTA TFPYVVLTIL FVRGVTLEGA FDGIMYYLTP QWDKILEAKV 370 380 390 400 410 420 WGDAASQIFY SLGCAWGGLI TMASYNKFHN NCYRDSVIIS ITNCATSVYA GFVIFSILGF 430 440 450 460 470 480 MANHLGVDVS RVADHGPGLA FVAYPEALTL LPISPLWSLL FFFMLILLGL GTQFCLLETL 490 500 510 520 530 540 VTAIVDEVGN EWILQKKTYV TLGVAVAGFL LGIPLTSQAG IYWLLLMDNY AASFSLVVIS 550 560 570 580 590 600 CIMCVAIMYI YGHRNYFQDI QMMLGFPPPL FFQICWRFVS PAIIFFILVF TVIQYQPITY 610 620 630 640 650 660 NHYQYPGWAV AIGFLMALSS VLCIPLYAMF RLCRTDGDTL LQRLKNATKP SRDWGPALLE 670 680 690 700 HRTGRYAPTI APSPEDGFEV QPLHPDKAQI PIVGSNGSSR LQDSRI
	Isoform GlyT-1A [UniParc]. Checksum: 7D72C44BC76E80EA	FASTA	633	70,583
	10 20 30 40 50 60 MVGKGAKGML NGAVPSEATK RDQNLKRGNW GNQIEFVLTS VGYAVGLGNV WRFPYLCYRN 70 80 90 100 110 120 GGGAFMFPYF IMLIFCGIPL FFMELSFGQF ASQGCLGVWR ISPMFKGVGY GMMVVSTYIG 130 140 150 160 170 180 IYYNVVICIA FYYFFSSMTH VLPWAYCNNP WNTHDCAGVL DASNLTNGSR PAALPSNLSH 190 200 210 220 230 240 LLNHSLQRTS PSEEYWRLYV LKLSDDIGNF GEVRLPLLGC LGVSWLVVFL CLIRGVKSSG 250 260 270 280 290 300 KVVYFTATFP YVVLTILFVR GVTLEGAFDG IMYYLTPQWD KILEAKVWGD AASQIFYSLG 310 320 330 340 350 360 CAWGGLITMA SYNKFHNNCY RDSVIISITN CATSVYAGFV IFSILGFMAN HLGVDVSRVA 370 380 390 400 410 420 DHGPGLAFVA YPEALTLLPI SPLWSLLFFF MLILLGLGTQ FCLLETLVTA IVDEVGNEWI 430 440 450 460 470 480 LQKKTYVTLG VAVAGFLLGI PLTSQAGIYW LLLMDNYAAS FSLVVISCIM CVAIMYIYGH 490 500 510 520 530 540 RNYFQDIQMM LGFPPPLFFQ ICWRFVSPAI IFFILVFTVI QYQPITYNHY QYPGWAVAIG 550 560 570 580 590 600 FLMALSSVLC IPLYAMFRLC RTDGDTLLQR LKNATKPSRD WGPALLEHRT GRYAPTIAPS 610 620 630 PEDGFEVQPL HPDKAQIPIV GSNGSSRLQD SRI
	Isoform GlyT-1B [UniParc]. Checksum: AA1FDD1DF09E7BCA	FASTA	652	72,482
	10 20 30 40 50 60 MSGGDTRAAI ARPRMAAAHG PVAPSSPEQN GAVPSEATKR DQNLKRGNWG NQIEFVLTSV 70 80 90 100 110 120 GYAVGLGNVW RFPYLCYRNG GGAFMFPYFI MLIFCGIPLF FMELSFGQFA SQGCLGVWRI 130 140 150 160 170 180 SPMFKGVGYG MMVVSTYIGI YYNVVICIAF YYFFSSMTHV LPWAYCNNPW NTHDCAGVLD 190 200 210 220 230 240 ASNLTNGSRP AALPSNLSHL LNHSLQRTSP SEEYWRLYVL KLSDDIGNFG EVRLPLLGCL 250 260 270 280 290 300 GVSWLVVFLC LIRGVKSSGK VVYFTATFPY VVLTILFVRG VTLEGAFDGI MYYLTPQWDK 310 320 330 340 350 360 ILEAKVWGDA ASQIFYSLGC AWGGLITMAS YNKFHNNCYR DSVIISITNC ATSVYAGFVI 370 380 390 400 410 420 FSILGFMANH LGVDVSRVAD HGPGLAFVAY PEALTLLPIS PLWSLLFFFM LILLGLGTQF 430 440 450 460 470 480 CLLETLVTAI VDEVGNEWIL QKKTYVTLGV AVAGFLLGIP LTSQAGIYWL LLMDNYAASF 490 500 510 520 530 540 SLVVISCIMC VAIMYIYGHR NYFQDIQMML GFPPPLFFQI CWRFVSPAII FFILVFTVIQ 550 560 570 580 590 600 YQPITYNHYQ YPGWAVAIGF LMALSSVLCI PLYAMFRLCR TDGDTLLQRL KNATKPSRDW 610 620 630 640 650 GPALLEHRTG RYAPTIAPSP EDGFEVQPLH PDKAQIPIVG SNGSSRLQDS RI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the human glycine transporter type 1: molecular and pharmacological characterization of novel isoform variants and chromosomal localization of the gene in the human and mouse genomes." Kim K.-M., Kingsmore S.F., Han H., Yang-Feng T.L., Godinot N., Seldin M.F., Caron M.G., Giros B. Mol. Pharmacol. 45:608-617(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS GLYT-1A; GLYT-1B AND GLYT-1C). Tissue: Brain.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S70609 mRNA. Translation: AAB30784.1. Frameshift. S70612 mRNA. Translation: AAB30785.1. Frameshift. AL139220 Genomic DNA. Translation: CAI19428.1. Different initiation. AL139220 Genomic DNA. Translation: CAI19429.1. Different initiation. AL139220 Genomic DNA. Translation: CAI19430.1. CH471059 Genomic DNA. Translation: EAX07056.1. CH471059 Genomic DNA. Translation: EAX07057.1.
IPI	IPI00216227. IPI00217065. IPI00217068.
PIR	I57956. I77912.
RefSeq	NP_001020016.1. NM_001024845.2. NP_008865.2. NM_006934.3. NP_964012.2. NM_201649.3.
UniGene	Hs.442590.
3D structure databases
ProteinModelPortal	P48067.
SMR	P48067. Positions 98-589.
ModBase	Search...
Protein-protein interaction databases
IntAct	P48067. 1 interaction.
STRING	9606.ENSP00000361381.
PTM databases
PhosphoSite	P48067.
Polymorphism databases
DMDM	302393807.
Proteomic databases
PRIDE	P48067.
Protocols and materials databases
DNASU	6536.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000357730; ENSP00000350362; ENSG00000196517. ENST00000360584; ENSP00000353791; ENSG00000196517. ENST00000372310; ENSP00000361384; ENSG00000196517.
GeneID	6536.
KEGG	hsa:6536.
UCSC	uc001cll.3. human. uc001clm.3. human. uc001cln.3. human.
Organism-specific databases
CTD	6536.
GeneCards	GC01M044462.
HGNC	HGNC:11056. SLC6A9.
HPA	HPA013977.
MIM	601019. gene.
neXtProt	NX_P48067.
PharmGKB	PA35916.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0733.
HOVERGEN	HBG071421.
InParanoid	P48067.
KO	K05038.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_20679. Amine compound SLC transporters.
Gene expression databases
ArrayExpress	P48067.
Bgee	P48067.
CleanEx	HS_SLC6A9.
Genevestigator	P48067.
GermOnline	ENSG00000196517. Homo sapiens.
Family and domain databases
InterPro	IPR000175. Na/ntran_symport. IPR003028. Na/ntran_symport_glycine_GLY1. [Graphical view]
PANTHER	PTHR11616. PTHR11616. 1 hit.
Pfam	PF00209. SNF. 1 hit. [Graphical view]
PRINTS	PR01204. GLY1TRNSPORT. PR00176. NANEUSMPORT.
PROSITE	PS00610. NA_NEUROTRAN_SYMP_1. 1 hit. PS00754. NA_NEUROTRAN_SYMP_2. 1 hit. PS50267. NA_NEUROTRAN_SYMP_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P48067.
ChEMBL	CHEMBL2337.
ChiTaRS	SLC6A9. human.
DrugBank	DB00145. Glycine.
GenomeRNAi	6536.
NextBio	25431.
SOURCE	Search...

Entry information

Entry name

SC6A9_HUMAN

Accession

Primary (citable) accession number: P48067
Secondary accession number(s): A6NDH1 , A6NII2, A6NNZ8, Q5TAB8, Q5TAB9, Q5TAC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	August 10, 2010
Last modified:	May 1, 2013
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.