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Protein

Carboxypeptidase A2

Gene

CPA2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Similar to that of carboxypeptidase A (EC 3.4.17.1), but with a preference for bulkier C-terminal residues.

Cofactori

Zn2+2 PublicationsNote: Binds 1 zinc ion per subunit.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi179Zinc; catalytic2 Publications1
Metal bindingi182Zinc; catalytic2 Publications1
Binding sitei237SubstrateBy similarity1
Metal bindingi306Zinc; catalytic2 Publications1
Binding sitei358SubstrateBy similarity1
Active sitei380Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • carboxypeptidase activity Source: UniProtKB
  • metallocarboxypeptidase activity Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • protein catabolic process in the vacuole Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS08303-MONOMER.
SABIO-RKP48052.

Protein family/group databases

MEROPSiM14.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase A2 (EC:3.4.17.15)
Gene namesi
Name:CPA2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:2297. CPA2.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
  • extracellular space Source: GO_Central
  • vacuole Source: GOC
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi1358.
OpenTargetsiENSG00000158516.
PharmGKBiPA26817.

Polymorphism and mutation databases

BioMutaiCPA2.
DMDMi294862522.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
PropeptideiPRO_000000435319 – 114Activation peptide1 PublicationAdd BLAST96
ChainiPRO_0000004354115 – 419Carboxypeptidase A2Add BLAST305

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi248 ↔ 2712 Publications
Disulfide bondi320 ↔ 3542 Publications

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP48052.
PeptideAtlasiP48052.
PRIDEiP48052.

PTM databases

iPTMnetiP48052.
PhosphoSitePlusiP48052.

Expressioni

Gene expression databases

BgeeiENSG00000158516.
CleanExiHS_CPA2.
ExpressionAtlasiP48052. baseline and differential.
GenevisibleiP48052. HS.

Organism-specific databases

HPAiHPA020342.
HPA021317.

Interactioni

Protein-protein interaction databases

BioGridi107750. 7 interactors.
MINTiMINT-125621.
STRINGi9606.ENSP00000222481.

Structurei

Secondary structure

1419
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 30Combined sources5
Helixi35 – 46Combined sources12
Helixi48 – 50Combined sources3
Beta strandi53 – 56Combined sources4
Beta strandi65 – 69Combined sources5
Helixi71 – 73Combined sources3
Helixi74 – 83Combined sources10
Beta strandi88 – 93Combined sources6
Helixi95 – 112Combined sources18
Turni113 – 115Combined sources3
Beta strandi120 – 122Combined sources3
Helixi126 – 139Combined sources14
Turni141 – 143Combined sources3
Beta strandi144 – 151Combined sources8
Beta strandi157 – 163Combined sources7
Beta strandi166 – 168Combined sources3
Beta strandi171 – 175Combined sources5
Helixi183 – 199Combined sources17
Turni200 – 202Combined sources3
Helixi204 – 212Combined sources9
Beta strandi214 – 219Combined sources6
Helixi223 – 231Combined sources9
Helixi253 – 255Combined sources3
Beta strandi257 – 260Combined sources4
Beta strandi263 – 267Combined sources5
Helixi284 – 296Combined sources13
Beta strandi299 – 306Combined sources8
Beta strandi311 – 315Combined sources5
Beta strandi317 – 319Combined sources3
Helixi326 – 341Combined sources16
Turni342 – 344Combined sources3
Beta strandi349 – 352Combined sources4
Helixi353 – 356Combined sources4
Helixi364 – 371Combined sources8
Beta strandi375 – 380Combined sources6
Beta strandi384 – 387Combined sources4
Helixi393 – 395Combined sources3
Helixi396 – 416Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AYEX-ray1.80A19-419[»]
1DTDX-ray1.65A118-419[»]
1O6XNMR-A19-96[»]
ProteinModelPortaliP48052.
SMRiP48052.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP48052.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni179 – 182Substrate bindingBy similarity4
Regioni254 – 255Substrate bindingBy similarity2
Regioni307 – 308Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOVERGENiHBG050815.
InParanoidiP48052.
KOiK01298.
OMAiAIMEHVR.
OrthoDBiEOG091G0HUI.
PhylomeDBiP48052.
TreeFamiTF317197.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR003146. M14A_act_pep.
IPR000834. Peptidase_M14.
IPR009020. Propept_inh.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48052-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMRLILFFG ALFGHIYCLE TFVGDQVLEI VPSNEEQIKN LLQLEAQEHL
60 70 80 90 100
QLDFWKSPTT PGETAHVRVP FVNVQAVKVF LESQGIAYSI MIEDVQVLLD
110 120 130 140 150
KENEEMLFNR RRERSGNFNF GAYHTLEEIS QEMDNLVAEH PGLVSKVNIG
160 170 180 190 200
SSFENRPMNV LKFSTGGDKP AIWLDAGIHA REWVTQATAL WTANKIVSDY
210 220 230 240 250
GKDPSITSIL DALDIFLLPV TNPDGYVFSQ TKNRMWRKTR SKVSGSLCVG
260 270 280 290 300
VDPNRNWDAG FGGPGASSNP CSDSYHGPSA NSEVEVKSIV DFIKSHGKVK
310 320 330 340 350
AFITLHSYSQ LLMFPYGYKC TKLDDFDELS EVAQKAAQSL RSLHGTKYKV
360 370 380 390 400
GPICSVIYQA SGGSIDWSYD YGIKYSFAFE LRDTGRYGFL LPARQILPTA
410
EETWLGLKAI MEHVRDHPY
Length:419
Mass (Da):47,030
Last modified:April 20, 2010 - v3
Checksum:i00269F2AE50CA38D
GO

Sequence cautioni

The sequence AAA74425 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH07009 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH14571 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH15140 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence EAL24092 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19L → S AA sequence (PubMed:2920728).Curated1
Sequence conflicti39K → N AA sequence (PubMed:2920728).Curated1
Sequence conflicti304T → I in AAA74425 (PubMed:7896805).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03120482E → G.2 PublicationsCorresponds to variant rs17850135dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC024085 Genomic DNA. No translation available.
CH236950 Genomic DNA. Translation: EAL24092.1. Sequence problems.
BC007009 mRNA. Translation: AAH07009.1. Different initiation.
BC014571 mRNA. Translation: AAH14571.1. Different initiation.
BC015140 mRNA. Translation: AAH15140.1. Different initiation.
U19977 mRNA. Translation: AAA74425.1. Different initiation.
BT007403 mRNA. Translation: AAP36067.1.
CCDSiCCDS5817.2.
PIRiA56171.
RefSeqiNP_001860.2. NM_001869.2.
UniGeneiHs.490038.

Genome annotation databases

EnsembliENST00000222481; ENSP00000222481; ENSG00000158516.
GeneIDi1358.
KEGGihsa:1358.
UCSCiuc003vpq.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC024085 Genomic DNA. No translation available.
CH236950 Genomic DNA. Translation: EAL24092.1. Sequence problems.
BC007009 mRNA. Translation: AAH07009.1. Different initiation.
BC014571 mRNA. Translation: AAH14571.1. Different initiation.
BC015140 mRNA. Translation: AAH15140.1. Different initiation.
U19977 mRNA. Translation: AAA74425.1. Different initiation.
BT007403 mRNA. Translation: AAP36067.1.
CCDSiCCDS5817.2.
PIRiA56171.
RefSeqiNP_001860.2. NM_001869.2.
UniGeneiHs.490038.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AYEX-ray1.80A19-419[»]
1DTDX-ray1.65A118-419[»]
1O6XNMR-A19-96[»]
ProteinModelPortaliP48052.
SMRiP48052.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107750. 7 interactors.
MINTiMINT-125621.
STRINGi9606.ENSP00000222481.

Protein family/group databases

MEROPSiM14.002.

PTM databases

iPTMnetiP48052.
PhosphoSitePlusiP48052.

Polymorphism and mutation databases

BioMutaiCPA2.
DMDMi294862522.

Proteomic databases

PaxDbiP48052.
PeptideAtlasiP48052.
PRIDEiP48052.

Protocols and materials databases

DNASUi1358.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000222481; ENSP00000222481; ENSG00000158516.
GeneIDi1358.
KEGGihsa:1358.
UCSCiuc003vpq.4. human.

Organism-specific databases

CTDi1358.
DisGeNETi1358.
GeneCardsiCPA2.
HGNCiHGNC:2297. CPA2.
HPAiHPA020342.
HPA021317.
MIMi600688. gene.
neXtProtiNX_P48052.
OpenTargetsiENSG00000158516.
PharmGKBiPA26817.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOVERGENiHBG050815.
InParanoidiP48052.
KOiK01298.
OMAiAIMEHVR.
OrthoDBiEOG091G0HUI.
PhylomeDBiP48052.
TreeFamiTF317197.

Enzyme and pathway databases

BioCyciZFISH:HS08303-MONOMER.
SABIO-RKP48052.

Miscellaneous databases

EvolutionaryTraceiP48052.
GeneWikiiCarboxypeptidase_A2.
GenomeRNAii1358.
PROiP48052.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000158516.
CleanExiHS_CPA2.
ExpressionAtlasiP48052. baseline and differential.
GenevisibleiP48052. HS.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR003146. M14A_act_pep.
IPR000834. Peptidase_M14.
IPR009020. Propept_inh.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBPA2_HUMAN
AccessioniPrimary (citable) accession number: P48052
Secondary accession number(s): A4D1M4
, C9JIK1, Q53XS1, Q96A12, Q96QN3, Q9UCF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: April 20, 2010
Last modified: November 2, 2016
This is version 167 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Peptidase families
    Classification of peptidase families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.