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P48051 (IRK6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G protein-activated inward rectifier potassium channel 2

Short name=GIRK-2
Alternative name(s):
BIR1
Inward rectifier K(+) channel Kir3.2
KATP-2
Potassium channel, inwardly rectifying subfamily J member 6
Gene names
Name:KCNJ6
Synonyms:GIRK2, KATP2, KCNJ7
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This potassium channel may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters acting through G-protein-coupled receptors. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.

Subunit structure

Associates with GIRK1 or GIRK4 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger. Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required when endocytosed to prevent degradation in lysosomes and promote recycling to the plasma membrane By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Most abundant in cerebellum, and to a lesser degree in islets and exocrine pancreas.

Sequence similarities

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ6 subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423G protein-activated inward rectifier potassium channel 2
PRO_0000154942

Regions

Topological domain1 – 8989Cytoplasmic By similarity
Transmembrane90 – 11425Helical; Name=M1; By similarity
Topological domain115 – 13824Extracellular By similarity
Intramembrane139 – 15012Helical; Pore-forming; Name=H5; By similarity
Intramembrane151 – 1577Pore-forming; By similarity
Topological domain158 – 1669Extracellular By similarity
Transmembrane167 – 18822Helical; Name=M2; By similarity
Topological domain189 – 423235Cytoplasmic By similarity
Motif152 – 1576Selectivity filter By similarity
Motif420 – 4234PDZ-binding

Sites

Site1821Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
P48051 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 7A02F6B0FBF8B7D4

FASTA42348,451
        10         20         30         40         50         60 
MAKLTESMTN VLEGDSMDQD VESPVAIHQP KLPKQARDDL PRHISRDRTK RKIQRYVRKD 

        70         80         90        100        110        120 
GKCNVHHGNV RETYRYLTDI FTTLVDLKWR FNLLIFVMVY TVTWLFFGMI WWLIAYIRGD 

       130        140        150        160        170        180 
MDHIEDPSWT PCVTNLNGFV SAFLFSIETE TTIGYGYRVI TDKCPEGIIL LLIQSVLGSI 

       190        200        210        220        230        240 
VNAFMVGCMF VKISQPKKRA ETLVFSTHAV ISMRDGKLCL MFRVGDLRNS HIVEASIRAK 

       250        260        270        280        290        300 
LIKSKQTSEG EFIPLNQTDI NVGYYTGDDR LFLVSPLIIS HEINQQSPFW EISKAQLPKE 

       310        320        330        340        350        360 
ELEIVVILEG MVEATGMTCQ ARSSYITSEI LWGYRFTPVL TLEDGFYEVD YNSFHETYET 

       370        380        390        400        410        420 
STPSLSAKEL AELASRAELP LSWSVSSKLN QHAELETEEE EKNLEEQTER NGDVANLENE 


SKV 

« Hide

References

« Hide 'large scale' references
[1]"Pancreatic islet cells express a family of inwardly rectifying K+ channel subunits which interact to form G-protein-activated channels."
Ferrer J., Nichols C.G., Makhina E.N., Salkoff L., Bernstein J., Gerhard D., Wasson J., Ramanadham S., Permutt A.
J. Biol. Chem. 270:26086-26091(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Insulinoma.
[2]"Subunit interactions in the assembly of neuronal G protein-activated inwardly rectifying K+ channels."
Dissmann E., Karschin A.
Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Hippocampus.
[3]"Gene identification in the 1.6 Mb of the Down syndrome region on chromosome 21."
Ohira M., Seki N., Nagase T., Suzuki E., Nomura N., Ohara O., Saito T., Ichikawa H., Ohki M.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[4]"Co-expression of human Kir3 subunits can yield channels with different functional properties."
Schoots O., Wilson J.M., Ethier N., Bigras E., Hebert T.E., Van Tol H.H.M.
Cell. Signal. 11:871-883(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Cerebellum.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[6]"Characterization and variation of a human inwardly-rectifying-K-channel gene (KCNJ6): a putative ATP-sensitive K-channel subunit."
Sakura H., Bond C., Warren-Perry M., Horsley S., Kearney L., Tucker S., Adelman J., Turner R., Ashcroft F.M.
FEBS Lett. 367:193-197(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 17-423.
[7]"Isolation of a cDNA clone encoding a KATP channel-like protein expressed in insulin-secreting cells, localization of the human gene to chromosome band 21q22.1, and linkage studies with NIDDM."
Tsaur M.-L., Menzel S., Lai F.-P., Espinosa R. III, Concannon P., Spielman R.S., Hanis C.L., Cox N.J., le Beau M.M., German M.S., Jan L.Y., Bell G.I., Stoffel M.
Diabetes 44:592-596(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 116-211.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U24660 mRNA. Translation: AAC50258.1.
L78480 mRNA. Translation: AAB02277.1.
D87327 mRNA. Translation: BAA13331.1.
U52153 mRNA. Translation: AAB07044.1.
BC101547 mRNA. Translation: AAI01548.1.
S78685, S78684 Genomic DNA. Translation: AAB34738.2.
G02354 Genomic DNA. No translation available.
PIRI38979.
RefSeqNP_002231.1. NM_002240.3.
UniGeneHs.626242.
Hs.658533.
Hs.741904.

3D structure databases

ProteinModelPortalP48051.
SMRP48051. Positions 53-378.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109965. 5 interactions.
STRING9606.ENSP00000383330.

Chemistry

DrugBankDB01159. Halothane.
GuidetoPHARMACOLOGY435.

Protein family/group databases

TCDB1.A.2.1.10. inward rectifier k(+) channel (irk-c) family.

PTM databases

PhosphoSiteP48051.

Polymorphism databases

DMDM1352487.

Proteomic databases

PaxDbP48051.
PRIDEP48051.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000288309; ENSP00000288309; ENSG00000157542.
ENST00000609713; ENSP00000477437; ENSG00000157542.
GeneID3763.
KEGGhsa:3763.
UCSCuc002ywo.3. human.

Organism-specific databases

CTD3763.
GeneCardsGC21M038996.
HGNCHGNC:6267. KCNJ6.
HPACAB012452.
MIM600877. gene.
neXtProtNX_P48051.
PharmGKBPA30049.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG72812.
HOGENOMHOG000237325.
HOVERGENHBG006178.
KOK05000.
OMATNAVISM.
OrthoDBEOG7XPZ5K.
PhylomeDBP48051.
TreeFamTF313676.

Enzyme and pathway databases

ReactomeREACT_13685. Neuronal System.

Gene expression databases

BgeeP48051.
CleanExHS_KCNJ6.
GenevestigatorP48051.

Family and domain databases

Gene3D2.60.40.1400. 1 hit.
InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003275. K_chnl_inward-rec_Kir3.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF19. PTHR11767:SF19. 1 hit.
PfamPF01007. IRK. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01328. KIR32CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMSSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

ChiTaRSKCNJ6. human.
GeneWikiKCNJ6.
GenomeRNAi3763.
NextBio14755.
PROP48051.
SOURCESearch...

Entry information

Entry nameIRK6_HUMAN
AccessionPrimary (citable) accession number: P48051
Secondary accession number(s): Q3MJ74, Q53WW6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 21

Human chromosome 21: entries, gene names and cross-references to MIM