Reviewed, UniProtKB/Swiss-Prot P48051 (IRK6_HUMAN)

Names and origin

Protein names

Recommended name:
    G protein-activated inward rectifier potassium channel 2
Alternative name(s):
    GIRK2
    Potassium channel, inwardly rectifying subfamily J member 6
    Inward rectifier K(+) channel Kir3.2
    KATP-2
    BIR1

Gene names

Name:	KCNJ6
Synonyms:	GIRK2, KATP2, KCNJ7

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This potassium channel may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters acting through G-protein-coupled receptors. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
Subunit structure	Associates with GIRK1 or GIRK4 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Most abundant in cerebellum, and to a lesser degree in islets and exocrine pancreas.
Sequence similarities	Belongs to the inward rectifier-type potassium channel family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane
Ligand	Potassium
Molecular function	Ionic channel Voltage-gated channel
Gene Ontology (GO)
Biological process	potassium ion transport Ref.6 Traceable author statement. Source: ProtInc
Cellular component	Golgi apparatus Inferred from direct assay. Source: UniProtKB voltage-gated potassium channel complex Ref.6 Traceable author statement. Source: ProtInc
Molecular function	G-protein activated inward rectifier potassium channel activity Traceable author statement. Source: ProtInc potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	G protein-activated inward rectifier potassium channel 2		PRO_0000154942
Regions
	Topological domain	1 – 89	89	Cytoplasmic By similarity
	Transmembrane	90 – 114	25	M1 By similarity
	Topological domain	115 – 138	24	Extracellular By similarity
	Topological domain	158 – 166	9	Extracellular By similarity
	Transmembrane	167 – 188	22	M2 By similarity
	Topological domain	189 – 423	235	Cytoplasmic By similarity
	Region	139 – 150	12	H5 (pore-forming helix) By similarity
	Motif	152 – 157	6	Selectivity filter By similarity
Sites
	Site	182	1	Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48051-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 7A02F6B0FBF8B7D4	FASTA	423	48,451
	10 20 30 40 50 60 MAKLTESMTN VLEGDSMDQD VESPVAIHQP KLPKQARDDL PRHISRDRTK RKIQRYVRKD 70 80 90 100 110 120 GKCNVHHGNV RETYRYLTDI FTTLVDLKWR FNLLIFVMVY TVTWLFFGMI WWLIAYIRGD 130 140 150 160 170 180 MDHIEDPSWT PCVTNLNGFV SAFLFSIETE TTIGYGYRVI TDKCPEGIIL LLIQSVLGSI 190 200 210 220 230 240 VNAFMVGCMF VKISQPKKRA ETLVFSTHAV ISMRDGKLCL MFRVGDLRNS HIVEASIRAK 250 260 270 280 290 300 LIKSKQTSEG EFIPLNQTDI NVGYYTGDDR LFLVSPLIIS HEINQQSPFW EISKAQLPKE 310 320 330 340 350 360 ELEIVVILEG MVEATGMTCQ ARSSYITSEI LWGYRFTPVL TLEDGFYEVD YNSFHETYET 370 380 390 400 410 420 STPSLSAKEL AELASRAELP LSWSVSSKLN QHAELETEEE EKNLEEQTER NGDVANLENE SKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pancreatic islet cells express a family of inwardly rectifying K+ channel subunits which interact to form G-protein-activated channels." Ferrer J., Nichols C.G., Makhina E.N., Salkoff L., Bernstein J., Gerhard D., Wasson J., Ramanadham S., Permutt A. J. Biol. Chem. 270:26086-26091(1995) [PubMed: 7592809] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Insulinoma.
[2]	"Subunit interactions in the assembly of neuronal G protein-activated inwardly rectifying K+ channels." Dissmann E., Karschin A. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Hippocampus.
[3]	"Gene identification in the 1.6 Mb of the Down syndrome region on chromosome 21." Ohira M., Seki N., Nagase T., Suzuki E., Nomura N., Ohara O., Saito T., Ichikawa H., Ohki M. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[4]	"Co-expression of human Kir3 subunits can yield channels with different functional properties." Schoots O., Wilson J.M., Ethier N., Bigras E., Hebert T.E., Van Tol H.H.M. Cell. Signal. 11:871-883(1999) [PubMed: 10659995] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cerebellum.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[6]	"Characterization and variation of a human inwardly-rectifying-K-channel gene (KCNJ6): a putative ATP-sensitive K-channel subunit." Sakura H., Bond C., Warren-Perry M., Horsley S., Kearney L., Tucker S., Adelman J., Turner R., Ashcroft F.M. FEBS Lett. 367:193-197(1995) [PubMed: 7796919] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 17-423.
[7]	"Isolation of a cDNA clone encoding a KATP channel-like protein expressed in insulin-secreting cells, localization of the human gene to chromosome band 21q22.1, and linkage studies with NIDDM." Tsaur M.-L., Menzel S., Lai F.-P., Espinosa R. III, Concannon P., Spielman R.S., Hanis C.L., Cox N.J., le Beau M.M., German M.S., Jan L.Y., Bell G.I., Stoffel M. Diabetes 44:592-596(1995) [PubMed: 7729621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 116-211.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U24660 mRNA. Translation: AAC50258.1. L78480 mRNA. Translation: AAB02277.1. D87327 mRNA. Translation: BAA13331.1. U52153 mRNA. Translation: AAB07044.1. BC101547 mRNA. Translation: AAI01548.1. S78685, S78684 Genomic DNA. Translation: AAB34738.2. G02354 Genomic DNA. No translation available.
IPI	IPI00007627.
PIR	I38979.
RefSeq	NP_002231.1.
UniGene	Hs.658533
3D structure databases
HSSP	HSSP built from PDB template 1N9P based on UniProtKB P35562.
ModBase	Search...
PTM databases
PhosphoSite	P48051.
Proteomic databases
PRIDE	P48051.
Genome annotation databases
Ensembl	ENSG00000157542. Homo sapiens. [Contig view]
GeneID	3763.
KEGG	hsa:3763.
Organism-specific databases
GeneCards	GC21M037918.
H-InvDB	HIX0040889.
HGNC	HGNC:6267. KCNJ6.
HPA	CAB012452.
MIM	600877. gene.
PharmGKB	PA30049.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P48051.
HOVERGEN	P48051.
Gene expression databases
ArrayExpress	P48051.
Bgee	P48051.
CleanEx	HS_KCNJ6.
GermOnline	ENSG00000157542. Homo sapiens.
Family and domain databases
InterPro	IPR016449. K_chnl_inward-rec_Kir. IPR001838. K_chnl_inward-rec_Kir-like. IPR003275. K_chnl_inward-rec_Kir3.2. IPR013521. K_chnl_inward-rec_Kir_Cr2. IPR013518. K_chnl_inward-rec_Kir_cyto. [Graphical view]
Gene3D	G3DSA:2.60.40.1400. IR_K+channel_cytopl. 1 hit.
PANTHER	PTHR11767. K+channel_IR. 1 hit. PTHR11767:SF19. KIR32_channel. 1 hit.
Pfam	PF01007. IRK. 1 hit. [Graphical view]
PIRSF	PIRSF005465. GIRK_kir. 1 hit.
PRINTS	PR01328. KIR32CHANNEL. PR01320. KIRCHANNEL.
ProDom	PD001103. K+channel_IR. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other Resources
DrugBank	DB01159. Halothane.
NextBio	14755.
SOURCE	Search...

Entry information

Entry name

IRK6_HUMAN

Accession

Primary (citable) accession number: P48051
Secondary accession number(s): Q3MJ74, Q53WW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)