P48051 (IRK6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G protein-activated inward rectifier potassium channel 2
Short name=GIRK-2

Alternative name(s):
BIR1
Inward rectifier K(+) channel Kir3.2
KATP-2
Potassium channel, inwardly rectifying subfamily J member 6

Gene names

Name:	KCNJ6
Synonyms:	GIRK2, KATP2, KCNJ7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This potassium channel may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters acting through G-protein-coupled receptors. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
Subunit structure	Associates with GIRK1 or GIRK4 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Most abundant in cerebellum, and to a lesser degree in islets and exocrine pancreas.
Sequence similarities	Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ6 subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Voltage-gated channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	synaptic transmission Traceable author statement. Source: Reactome
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 16780588. Source: UniProtKB voltage-gated potassium channel complex Traceable author statement Ref.6. Source: ProtInc
Molecular_function	G-protein activated inward rectifier potassium channel activity Traceable author statement PubMed 7550338. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	G protein-activated inward rectifier potassium channel 2		PRO_0000154942
Regions
	Topological domain	1 – 89	89	Cytoplasmic By similarity
	Transmembrane	90 – 114	25	Helical; Name=M1; By similarity
	Topological domain	115 – 138	24	Extracellular By similarity
	Intramembrane	139 – 150	12	Helical; Pore-forming; Name=H5; By similarity
	Intramembrane	151 – 157	7	Pore-forming; By similarity
	Topological domain	158 – 166	9	Extracellular By similarity
	Transmembrane	167 – 188	22	Helical; Name=M2; By similarity
	Topological domain	189 – 423	235	Cytoplasmic By similarity
	Motif	152 – 157	6	Selectivity filter By similarity
Sites
	Site	182	1	Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48051 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 7A02F6B0FBF8B7D4	FASTA	423	48,451
	10 20 30 40 50 60 MAKLTESMTN VLEGDSMDQD VESPVAIHQP KLPKQARDDL PRHISRDRTK RKIQRYVRKD 70 80 90 100 110 120 GKCNVHHGNV RETYRYLTDI FTTLVDLKWR FNLLIFVMVY TVTWLFFGMI WWLIAYIRGD 130 140 150 160 170 180 MDHIEDPSWT PCVTNLNGFV SAFLFSIETE TTIGYGYRVI TDKCPEGIIL LLIQSVLGSI 190 200 210 220 230 240 VNAFMVGCMF VKISQPKKRA ETLVFSTHAV ISMRDGKLCL MFRVGDLRNS HIVEASIRAK 250 260 270 280 290 300 LIKSKQTSEG EFIPLNQTDI NVGYYTGDDR LFLVSPLIIS HEINQQSPFW EISKAQLPKE 310 320 330 340 350 360 ELEIVVILEG MVEATGMTCQ ARSSYITSEI LWGYRFTPVL TLEDGFYEVD YNSFHETYET 370 380 390 400 410 420 STPSLSAKEL AELASRAELP LSWSVSSKLN QHAELETEEE EKNLEEQTER NGDVANLENE SKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pancreatic islet cells express a family of inwardly rectifying K+ channel subunits which interact to form G-protein-activated channels." Ferrer J., Nichols C.G., Makhina E.N., Salkoff L., Bernstein J., Gerhard D., Wasson J., Ramanadham S., Permutt A. J. Biol. Chem. 270:26086-26091(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Insulinoma.
[2]	"Subunit interactions in the assembly of neuronal G protein-activated inwardly rectifying K+ channels." Dissmann E., Karschin A. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Hippocampus.
[3]	"Gene identification in the 1.6 Mb of the Down syndrome region on chromosome 21." Ohira M., Seki N., Nagase T., Suzuki E., Nomura N., Ohara O., Saito T., Ichikawa H., Ohki M. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[4]	"Co-expression of human Kir3 subunits can yield channels with different functional properties." Schoots O., Wilson J.M., Ethier N., Bigras E., Hebert T.E., Van Tol H.H.M. Cell. Signal. 11:871-883(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cerebellum.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[6]	"Characterization and variation of a human inwardly-rectifying-K-channel gene (KCNJ6): a putative ATP-sensitive K-channel subunit." Sakura H., Bond C., Warren-Perry M., Horsley S., Kearney L., Tucker S., Adelman J., Turner R., Ashcroft F.M. FEBS Lett. 367:193-197(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 17-423.
[7]	"Isolation of a cDNA clone encoding a KATP channel-like protein expressed in insulin-secreting cells, localization of the human gene to chromosome band 21q22.1, and linkage studies with NIDDM." Tsaur M.-L., Menzel S., Lai F.-P., Espinosa R. III, Concannon P., Spielman R.S., Hanis C.L., Cox N.J., le Beau M.M., German M.S., Jan L.Y., Bell G.I., Stoffel M. Diabetes 44:592-596(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 116-211.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U24660 mRNA. Translation: AAC50258.1. L78480 mRNA. Translation: AAB02277.1. D87327 mRNA. Translation: BAA13331.1. U52153 mRNA. Translation: AAB07044.1. BC101547 mRNA. Translation: AAI01548.1. S78685, S78684 Genomic DNA. Translation: AAB34738.2. G02354 Genomic DNA. No translation available.
IPI	IPI00007627.
PIR	I38979.
RefSeq	NP_002231.1. NM_002240.3.
UniGene	Hs.658533. Hs.741904.
3D structure databases
ProteinModelPortal	P48051.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000383330.
PTM databases
PhosphoSite	P48051.
Polymorphism databases
DMDM	1352487.
Proteomic databases
PaxDb	P48051.
PRIDE	P48051.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000288309; ENSP00000288309; ENSG00000157542. ENST00000400482; ENSP00000383330; ENSG00000157542.
GeneID	3763.
KEGG	hsa:3763.
UCSC	uc002ywo.2. human.
Organism-specific databases
CTD	3763.
GeneCards	GC21M038996.
HGNC	HGNC:6267. KCNJ6.
HPA	CAB012452.
MIM	600877. gene.
neXtProt	NX_P48051.
PharmGKB	PA30049.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG72812.
HOGENOM	HOG000237325.
HOVERGEN	HBG006178.
KO	K05000.
OMA	TNAVISM.
OrthoDB	EOG4VT5X9.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System.
Gene expression databases
Bgee	P48051.
CleanEx	HS_KCNJ6.
Genevestigator	P48051.
GermOnline	ENSG00000157542. Homo sapiens.
Family and domain databases
Gene3D	2.60.40.1400. 1 hit.
InterPro	IPR014756. Ig_E-set. IPR016449. K_chnl_inward-rec_Kir. IPR003275. K_chnl_inward-rec_Kir3.2. IPR013518. K_chnl_inward-rec_Kir_cyto. [Graphical view]
PANTHER	PTHR11767. PTHR11767. 1 hit. PTHR11767:SF19. PTHR11767:SF19. 1 hit.
Pfam	PF01007. IRK. 1 hit. [Graphical view]
PIRSF	PIRSF005465. GIRK_kir. 1 hit.
PRINTS	PR01328. KIR32CHANNEL. PR01320. KIRCHANNEL.
SUPFAM	SSF81296. Ig_E-set. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	KCNJ6. human.
DrugBank	DB01159. Halothane.
GenomeRNAi	3763.
NextBio	14755.
SOURCE	Search...

Entry information

Entry name

IRK6_HUMAN

Accession

Primary (citable) accession number: P48051
Secondary accession number(s): Q3MJ74, Q53WW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.