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P48051

- KCNJ6_HUMAN

UniProt

P48051 - KCNJ6_HUMAN

Protein

G protein-activated inward rectifier potassium channel 2

Gene

KCNJ6

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    This potassium channel may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters acting through G-protein-coupled receptors. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei182 – 1821Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

    GO - Molecular functioni

    1. G-protein activated inward rectifier potassium channel activity Source: ProtInc
    2. inward rectifier potassium channel activity Source: ProtInc
    3. protein binding Source: BHF-UCL

    GO - Biological processi

    1. potassium ion transmembrane transport Source: GOC
    2. potassium ion transport Source: ProtInc
    3. synaptic transmission Source: Reactome

    Keywords - Molecular functioni

    Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_25004. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
    REACT_75831. Activation of G protein gated Potassium channels.

    Protein family/group databases

    TCDBi1.A.2.1.10. inward rectifier k(+) channel (irk-c) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G protein-activated inward rectifier potassium channel 2
    Short name:
    GIRK-2
    Alternative name(s):
    BIR1
    Inward rectifier K(+) channel Kir3.2
    KATP-2
    Potassium channel, inwardly rectifying subfamily J member 6
    Gene namesi
    Name:KCNJ6
    Synonyms:GIRK2, KATP2, KCNJ7
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 21

    Organism-specific databases

    HGNCiHGNC:6267. KCNJ6.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi apparatus Source: UniProtKB
    2. plasma membrane Source: UniProtKB
    3. voltage-gated potassium channel complex Source: ProtInc

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30049.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 423423G protein-activated inward rectifier potassium channel 2PRO_0000154942Add
    BLAST

    Proteomic databases

    PaxDbiP48051.
    PRIDEiP48051.

    PTM databases

    PhosphoSiteiP48051.

    Expressioni

    Tissue specificityi

    Most abundant in cerebellum, and to a lesser degree in islets and exocrine pancreas.

    Gene expression databases

    BgeeiP48051.
    CleanExiHS_KCNJ6.
    GenevestigatoriP48051.

    Organism-specific databases

    HPAiCAB012452.

    Interactioni

    Subunit structurei

    Associates with GIRK1 or GIRK4 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger. Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required when endocytosed to prevent degradation in lysosomes and promote recycling to the plasma membrane By similarity.By similarity

    Protein-protein interaction databases

    BioGridi109965. 5 interactions.
    STRINGi9606.ENSP00000383330.

    Structurei

    3D structure databases

    ProteinModelPortaliP48051.
    SMRiP48051. Positions 53-378.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 8989CytoplasmicBy similarityAdd
    BLAST
    Topological domaini115 – 13824ExtracellularBy similarityAdd
    BLAST
    Topological domaini158 – 1669ExtracellularBy similarity
    Topological domaini189 – 423235CytoplasmicBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei139 – 15012Helical; Pore-forming; Name=H5By similarityAdd
    BLAST
    Intramembranei151 – 1577Pore-formingBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei90 – 11425Helical; Name=M1By similarityAdd
    BLAST
    Transmembranei167 – 18822Helical; Name=M2By similarityAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi152 – 1576Selectivity filterBy similarity
    Motifi420 – 4234PDZ-binding

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG72812.
    HOGENOMiHOG000237325.
    HOVERGENiHBG006178.
    KOiK05000.
    OMAiTNAVISM.
    OrthoDBiEOG7XPZ5K.
    PhylomeDBiP48051.
    TreeFamiTF313676.

    Family and domain databases

    Gene3Di2.60.40.1400. 1 hit.
    InterProiIPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003275. K_chnl_inward-rec_Kir3.2.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view]
    PANTHERiPTHR11767. PTHR11767. 1 hit.
    PTHR11767:SF19. PTHR11767:SF19. 1 hit.
    PfamiPF01007. IRK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005465. GIRK_kir. 1 hit.
    PRINTSiPR01328. KIR32CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P48051-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKLTESMTN VLEGDSMDQD VESPVAIHQP KLPKQARDDL PRHISRDRTK    50
    RKIQRYVRKD GKCNVHHGNV RETYRYLTDI FTTLVDLKWR FNLLIFVMVY 100
    TVTWLFFGMI WWLIAYIRGD MDHIEDPSWT PCVTNLNGFV SAFLFSIETE 150
    TTIGYGYRVI TDKCPEGIIL LLIQSVLGSI VNAFMVGCMF VKISQPKKRA 200
    ETLVFSTHAV ISMRDGKLCL MFRVGDLRNS HIVEASIRAK LIKSKQTSEG 250
    EFIPLNQTDI NVGYYTGDDR LFLVSPLIIS HEINQQSPFW EISKAQLPKE 300
    ELEIVVILEG MVEATGMTCQ ARSSYITSEI LWGYRFTPVL TLEDGFYEVD 350
    YNSFHETYET STPSLSAKEL AELASRAELP LSWSVSSKLN QHAELETEEE 400
    EKNLEEQTER NGDVANLENE SKV 423
    Length:423
    Mass (Da):48,451
    Last modified:February 1, 1996 - v1
    Checksum:i7A02F6B0FBF8B7D4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U24660 mRNA. Translation: AAC50258.1.
    L78480 mRNA. Translation: AAB02277.1.
    D87327 mRNA. Translation: BAA13331.1.
    U52153 mRNA. Translation: AAB07044.1.
    BC101547 mRNA. Translation: AAI01548.1.
    S78685, S78684 Genomic DNA. Translation: AAB34738.2.
    G02354 Genomic DNA. No translation available.
    CCDSiCCDS42927.1.
    PIRiI38979.
    RefSeqiNP_002231.1. NM_002240.3.
    UniGeneiHs.626242.
    Hs.658533.
    Hs.741904.

    Genome annotation databases

    EnsembliENST00000609713; ENSP00000477437; ENSG00000157542.
    GeneIDi3763.
    KEGGihsa:3763.
    UCSCiuc002ywo.3. human.

    Polymorphism databases

    DMDMi1352487.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U24660 mRNA. Translation: AAC50258.1 .
    L78480 mRNA. Translation: AAB02277.1 .
    D87327 mRNA. Translation: BAA13331.1 .
    U52153 mRNA. Translation: AAB07044.1 .
    BC101547 mRNA. Translation: AAI01548.1 .
    S78685 , S78684 Genomic DNA. Translation: AAB34738.2 .
    G02354 Genomic DNA. No translation available.
    CCDSi CCDS42927.1.
    PIRi I38979.
    RefSeqi NP_002231.1. NM_002240.3.
    UniGenei Hs.626242.
    Hs.658533.
    Hs.741904.

    3D structure databases

    ProteinModelPortali P48051.
    SMRi P48051. Positions 53-378.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109965. 5 interactions.
    STRINGi 9606.ENSP00000383330.

    Chemistry

    ChEMBLi CHEMBL3038490.
    DrugBanki DB01159. Halothane.
    GuidetoPHARMACOLOGYi 435.

    Protein family/group databases

    TCDBi 1.A.2.1.10. inward rectifier k(+) channel (irk-c) family.

    PTM databases

    PhosphoSitei P48051.

    Polymorphism databases

    DMDMi 1352487.

    Proteomic databases

    PaxDbi P48051.
    PRIDEi P48051.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000609713 ; ENSP00000477437 ; ENSG00000157542 .
    GeneIDi 3763.
    KEGGi hsa:3763.
    UCSCi uc002ywo.3. human.

    Organism-specific databases

    CTDi 3763.
    GeneCardsi GC21M038996.
    HGNCi HGNC:6267. KCNJ6.
    HPAi CAB012452.
    MIMi 600877. gene.
    neXtProti NX_P48051.
    PharmGKBi PA30049.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG72812.
    HOGENOMi HOG000237325.
    HOVERGENi HBG006178.
    KOi K05000.
    OMAi TNAVISM.
    OrthoDBi EOG7XPZ5K.
    PhylomeDBi P48051.
    TreeFami TF313676.

    Enzyme and pathway databases

    Reactomei REACT_25004. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
    REACT_75831. Activation of G protein gated Potassium channels.

    Miscellaneous databases

    ChiTaRSi KCNJ6. human.
    GeneWikii KCNJ6.
    GenomeRNAii 3763.
    NextBioi 14755.
    PROi P48051.
    SOURCEi Search...

    Gene expression databases

    Bgeei P48051.
    CleanExi HS_KCNJ6.
    Genevestigatori P48051.

    Family and domain databases

    Gene3Di 2.60.40.1400. 1 hit.
    InterProi IPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003275. K_chnl_inward-rec_Kir3.2.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view ]
    PANTHERi PTHR11767. PTHR11767. 1 hit.
    PTHR11767:SF19. PTHR11767:SF19. 1 hit.
    Pfami PF01007. IRK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005465. GIRK_kir. 1 hit.
    PRINTSi PR01328. KIR32CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Pancreatic islet cells express a family of inwardly rectifying K+ channel subunits which interact to form G-protein-activated channels."
      Ferrer J., Nichols C.G., Makhina E.N., Salkoff L., Bernstein J., Gerhard D., Wasson J., Ramanadham S., Permutt A.
      J. Biol. Chem. 270:26086-26091(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Insulinoma.
    2. "Subunit interactions in the assembly of neuronal G protein-activated inwardly rectifying K+ channels."
      Dissmann E., Karschin A.
      Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Hippocampus.
    3. "Gene identification in the 1.6 Mb of the Down syndrome region on chromosome 21."
      Ohira M., Seki N., Nagase T., Suzuki E., Nomura N., Ohara O., Saito T., Ichikawa H., Ohki M.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    4. "Co-expression of human Kir3 subunits can yield channels with different functional properties."
      Schoots O., Wilson J.M., Ethier N., Bigras E., Hebert T.E., Van Tol H.H.M.
      Cell. Signal. 11:871-883(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Cerebellum.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta.
    6. "Characterization and variation of a human inwardly-rectifying-K-channel gene (KCNJ6): a putative ATP-sensitive K-channel subunit."
      Sakura H., Bond C., Warren-Perry M., Horsley S., Kearney L., Tucker S., Adelman J., Turner R., Ashcroft F.M.
      FEBS Lett. 367:193-197(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 17-423.
    7. "Isolation of a cDNA clone encoding a KATP channel-like protein expressed in insulin-secreting cells, localization of the human gene to chromosome band 21q22.1, and linkage studies with NIDDM."
      Tsaur M.-L., Menzel S., Lai F.-P., Espinosa R. III, Concannon P., Spielman R.S., Hanis C.L., Cox N.J., le Beau M.M., German M.S., Jan L.Y., Bell G.I., Stoffel M.
      Diabetes 44:592-596(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 116-211.

    Entry informationi

    Entry nameiKCNJ6_HUMAN
    AccessioniPrimary (citable) accession number: P48051
    Secondary accession number(s): Q3MJ74, Q53WW6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 21
      Human chromosome 21: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3