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Protein

ATP synthase subunit O, mitochondrial

Gene

ATP5O

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary elements.

GO - Molecular functioni

  • drug binding Source: UniProtKB
  • proton-transporting ATP synthase activity, rotational mechanism Source: InterPro
  • transmembrane transporter activity Source: UniProtKB
  • transporter activity Source: ProtInc

GO - Biological processi

  • ATP biosynthetic process Source: UniProtKB
  • mitochondrial ATP synthesis coupled proton transport Source: UniProtKB
  • proton transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

ATP synthesis, Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

BioCyciZFISH:HS08368-MONOMER.
ReactomeiR-HSA-163210. Formation of ATP by chemiosmotic coupling.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP synthase subunit O, mitochondrial
Alternative name(s):
Oligomycin sensitivity conferral protein
Short name:
OSCP
Gene namesi
Name:ATP5O
Synonyms:ATPO
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 21

Organism-specific databases

HGNCiHGNC:850. ATP5O.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • extracellular matrix Source: BHF-UCL
  • mitochondrial inner membrane Source: Reactome
  • mitochondrial proton-transporting ATP synthase complex Source: UniProtKB
  • mitochondrion Source: UniProtKB
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi162K → R: Increased ATP levels. 1 Publication1

Organism-specific databases

DisGeNETi539.
OpenTargetsiENSG00000241837.
PharmGKBiPA25144.

Polymorphism and mutation databases

BioMutaiATP5O.
DMDMi1352049.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 23Mitochondrion1 PublicationAdd BLAST23
ChainiPRO_000000264624 – 213ATP synthase subunit O, mitochondrialAdd BLAST190

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54N6-acetyllysineBy similarity1
Modified residuei60N6-acetyllysineBy similarity1
Modified residuei70N6-acetyllysineBy similarity1
Modified residuei73N6-acetyllysineBy similarity1
Modified residuei90N6-succinyllysineBy similarity1
Modified residuei158N6-acetyllysine; alternateBy similarity1
Modified residuei158N6-succinyllysine; alternateBy similarity1
Modified residuei162N6-acetyllysine; alternateCombined sources1 Publication1
Modified residuei162N6-succinyllysine; alternateBy similarity1
Modified residuei172N6-acetyllysineCombined sources1
Modified residuei176N6-acetyllysineBy similarity1
Modified residuei192N6-acetyllysineCombined sources1
Modified residuei199N6-succinyllysineBy similarity1

Post-translational modificationi

Acetylation at Lys-162 decreases ATP production. Deacetylated by SIRT3.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP48047.
MaxQBiP48047.
PaxDbiP48047.
PeptideAtlasiP48047.
PRIDEiP48047.
TopDownProteomicsiP48047.

2D gel databases

OGPiP48047.
UCD-2DPAGEP48047.

PTM databases

iPTMnetiP48047.
PhosphoSitePlusiP48047.
SwissPalmiP48047.

Expressioni

Gene expression databases

BgeeiENSG00000241837.
CleanExiHS_ATP5O.
ExpressionAtlasiP48047. baseline and differential.
GenevisibleiP48047. HS.

Organism-specific databases

HPAiCAB016209.
HPA041394.

Interactioni

Subunit structurei

F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a, b and c. Component of an ATP synthase complex composed of ATP5F1, ATP5G1, ATP5E, ATP5H, ATP5I, ATP5J, ATP5J2, MT-ATP6, MT-ATP8, ATP5A1, ATP5B, ATP5D, ATP5C1, ATP5O, ATP5L, USMG5 and MP68 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CCDC136Q96JN2-23EBI-355815,EBI-10171416
CEP70Q8NHQ15EBI-355815,EBI-739624
HMBOX1Q6NT763EBI-355815,EBI-2549423
KRT13A1A4E93EBI-355815,EBI-10171552
KRT15P190123EBI-355815,EBI-739566
MIPOL1Q8TD103EBI-355815,EBI-2548751
NDC80O147773EBI-355815,EBI-715849
NT5C2P499023EBI-355815,EBI-742084
PNMA1Q8ND904EBI-355815,EBI-302345
TFIP11Q9UBB93EBI-355815,EBI-1105213
TRIP6Q156543EBI-355815,EBI-742327
USHBP1Q8N6Y03EBI-355815,EBI-739895
ZBTB14O438293EBI-355815,EBI-10176632

Protein-protein interaction databases

BioGridi107021. 101 interactors.
IntActiP48047. 54 interactors.
MINTiMINT-5002594.
STRINGi9606.ENSP00000290299.

Structurei

3D structure databases

ProteinModelPortaliP48047.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATPase delta chain family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1662. Eukaryota.
COG0712. LUCA.
GeneTreeiENSGT00390000015060.
HOGENOMiHOG000075825.
HOVERGENiHBG004309.
InParanoidiP48047.
KOiK02137.
OMAiMNPHVKR.
OrthoDBiEOG091G0L4M.
PhylomeDBiP48047.
TreeFamiTF106241.

Family and domain databases

Gene3Di1.10.520.20. 1 hit.
HAMAPiMF_01416. ATP_synth_delta_bact. 1 hit.
InterProiIPR026015. ATP_synth_OSCP/delta_N.
IPR020781. ATPase_OSCP/d_CS.
IPR000711. ATPase_OSCP/dsu.
[Graphical view]
PANTHERiPTHR11910. PTHR11910. 1 hit.
PfamiPF00213. OSCP. 1 hit.
[Graphical view]
PRINTSiPR00125. ATPASEDELTA.
SUPFAMiSSF47928. SSF47928. 1 hit.
TIGRFAMsiTIGR01145. ATP_synt_delta. 1 hit.
PROSITEiPS00389. ATPASE_DELTA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48047-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPAVSGLS RQVRCFSTSV VRPFAKLVRP PVQVYGIEGR YATALYSAAS
60 70 80 90 100
KQNKLEQVEK ELLRVAQILK EPKVAASVLN PYVKRSIKVK SLNDITAKER
110 120 130 140 150
FSPLTTNLIN LLAENGRLSN TQGVVSAFST MMSVHRGEVP CTVTSASPLE
160 170 180 190 200
EATLSELKTV LKSFLSQGQV LKLEAKTDPS ILGGMIVRIG EKYVDMSVKT
210
KIQKLGRAMR EIV
Length:213
Mass (Da):23,277
Last modified:February 1, 1996 - v1
Checksum:i311F53576A31FC93
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01193098K → R.2 PublicationsCorresponds to variant rs4842dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83218 mRNA. Translation: CAA58219.1.
BT019836 mRNA. Translation: AAV38639.1.
CR456822 mRNA. Translation: CAG33103.1.
AK222962 mRNA. Translation: BAD96682.1.
AK311796 mRNA. Translation: BAG34739.1.
CH471079 Genomic DNA. Translation: EAX09802.1.
BC021233 mRNA. Translation: AAH21233.1.
BC022865 mRNA. Translation: AAH22865.1.
CCDSiCCDS13634.1.
RefSeqiNP_001688.1. NM_001697.2.
UniGeneiHs.409140.

Genome annotation databases

EnsembliENST00000290299; ENSP00000290299; ENSG00000241837.
GeneIDi539.
KEGGihsa:539.
UCSCiuc002ytl.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83218 mRNA. Translation: CAA58219.1.
BT019836 mRNA. Translation: AAV38639.1.
CR456822 mRNA. Translation: CAG33103.1.
AK222962 mRNA. Translation: BAD96682.1.
AK311796 mRNA. Translation: BAG34739.1.
CH471079 Genomic DNA. Translation: EAX09802.1.
BC021233 mRNA. Translation: AAH21233.1.
BC022865 mRNA. Translation: AAH22865.1.
CCDSiCCDS13634.1.
RefSeqiNP_001688.1. NM_001697.2.
UniGeneiHs.409140.

3D structure databases

ProteinModelPortaliP48047.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107021. 101 interactors.
IntActiP48047. 54 interactors.
MINTiMINT-5002594.
STRINGi9606.ENSP00000290299.

PTM databases

iPTMnetiP48047.
PhosphoSitePlusiP48047.
SwissPalmiP48047.

Polymorphism and mutation databases

BioMutaiATP5O.
DMDMi1352049.

2D gel databases

OGPiP48047.
UCD-2DPAGEP48047.

Proteomic databases

EPDiP48047.
MaxQBiP48047.
PaxDbiP48047.
PeptideAtlasiP48047.
PRIDEiP48047.
TopDownProteomicsiP48047.

Protocols and materials databases

DNASUi539.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000290299; ENSP00000290299; ENSG00000241837.
GeneIDi539.
KEGGihsa:539.
UCSCiuc002ytl.4. human.

Organism-specific databases

CTDi539.
DisGeNETi539.
GeneCardsiATP5O.
H-InvDBHIX0158705.
HGNCiHGNC:850. ATP5O.
HPAiCAB016209.
HPA041394.
MIMi600828. gene.
neXtProtiNX_P48047.
OpenTargetsiENSG00000241837.
PharmGKBiPA25144.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1662. Eukaryota.
COG0712. LUCA.
GeneTreeiENSGT00390000015060.
HOGENOMiHOG000075825.
HOVERGENiHBG004309.
InParanoidiP48047.
KOiK02137.
OMAiMNPHVKR.
OrthoDBiEOG091G0L4M.
PhylomeDBiP48047.
TreeFamiTF106241.

Enzyme and pathway databases

BioCyciZFISH:HS08368-MONOMER.
ReactomeiR-HSA-163210. Formation of ATP by chemiosmotic coupling.

Miscellaneous databases

GeneWikiiATP5O.
GenomeRNAii539.
PROiP48047.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000241837.
CleanExiHS_ATP5O.
ExpressionAtlasiP48047. baseline and differential.
GenevisibleiP48047. HS.

Family and domain databases

Gene3Di1.10.520.20. 1 hit.
HAMAPiMF_01416. ATP_synth_delta_bact. 1 hit.
InterProiIPR026015. ATP_synth_OSCP/delta_N.
IPR020781. ATPase_OSCP/d_CS.
IPR000711. ATPase_OSCP/dsu.
[Graphical view]
PANTHERiPTHR11910. PTHR11910. 1 hit.
PfamiPF00213. OSCP. 1 hit.
[Graphical view]
PRINTSiPR00125. ATPASEDELTA.
SUPFAMiSSF47928. SSF47928. 1 hit.
TIGRFAMsiTIGR01145. ATP_synt_delta. 1 hit.
PROSITEiPS00389. ATPASE_DELTA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATPO_HUMAN
AccessioniPrimary (citable) accession number: P48047
Secondary accession number(s): B2R4E2, Q5U042, Q6IBI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 169 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.