P48036 (ANXA5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Annexin A5

Alternative name(s):
Anchorin CII
Annexin V
Annexin-5
Calphobindin I
Short name=CBP-I
Endonexin II
Lipocortin V
Placental anticoagulant protein 4
Short name=PP4
Placental anticoagulant protein I
Short name=PAP-I
Thromboplastin inhibitor
Vascular anticoagulant-alpha
Short name=VAC-alpha

Gene names

Name:	Anxa5
Synonyms:	Anx5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Subunit structure	Monomer. Binds ATRX and EIF5B By similarity.
Domain	A pair of annexin repeats may form one binding site for calcium and phospholipid.
Sequence similarities	Belongs to the annexin family. Contains 4 annexin repeats.

Ontologies

Keywords
Biological process	Blood coagulation
Domain	Annexin Repeat
Ligand	Calcium Calcium/phospholipid-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of coagulation Inferred from electronic annotation. Source: InterPro response to organic substance Inferred from direct assay. Source: MGI
Cellular component	endothelial microparticle Inferred from direct assay. Source: BHF-UCL external side of plasma membrane Inferred from direct assay. Source: MGI
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro calcium-dependent phospholipid binding Inferred from electronic annotation. Source: UniProtKB-KW eukaryotic cell surface binding Inferred from direct assay. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Kcnma1	Q08460	4	EBI-1184119,EBI-1633915

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Annexin A5		PRO_0000067488
Regions
	Repeat	22 – 82	61	Annexin 1
	Repeat	94 – 154	61	Annexin 2
	Repeat	178 – 238	61	Annexin 3
	Repeat	253 – 313	61	Annexin 4
Amino acid modifications
	Modified residue	68	1	N6-acetyllysine By similarity
	Modified residue	74	1	N6-acetyllysine By similarity
	Modified residue	77	1	N6-acetyllysine By similarity
	Modified residue	92	1	Phosphotyrosine By similarity
	Modified residue	95	1	N6-acetyllysine By similarity
	Modified residue	99	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	142	1	D → G in BAE28107. Ref.4
	Sequence conflict	157	1	A → G in AAH03716. Ref.5
	Sequence conflict	284	1	K → N in BAE31952. Ref.4
	Sequence conflict	300	1	G → S in BAE32026. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P48036 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 55055BAF2E1C36B7	FASTA	319	35,752
	10 20 30 40 50 60 MATRGTVTDF PGFDGRADAE VLRKAMKGLG TDEDSILNLL TSRSNAQRQE IAQEFKTLFG 70 80 90 100 110 120 RDLVDDLKSE LTGKFEKLIV AMMKPSRLYD AYELKHALKG AGTDEKVLTE IIASRTPEEL 130 140 150 160 170 180 SAIKQVYEEE YGSNLEDDVV GDTSGYYQRM LVVLLQANRD PDTAIDDAQV ELDAQALFQA 190 200 210 220 230 240 GELKWGTDEE KFITIFGTRS VSHLRRVFDK YMTISGFQIE ETIDRETSGN LEQLLLAVVK 250 260 270 280 290 300 SIRSIPAYLA ETLYYAMKGA GTDDHTLIRV VVSRSEIDLF NIRKEFRKNF ATSLYSMIKG 310 DTSGDYKKAL LLLCGGEDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse annexin V chromosomal localization, cDNA sequence conservation, and molecular evolution." Rodriguez-Garcia M.I., Kozak C.A., Morgan R.O., Fernandez M.-P. Genomics 31:151-157(1996) [PubMed: 8824796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Peritoneal cavity.
[2]	Adachi T., Kojima K., Fukuoka S., Ogawa H., Matsumoto I. Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Mouse annexin V genomic organization includes an endogenous retrovirus." Rodriguez-Garcia M.I., Morgan R.O., Fernandez M.R., Bances P., Fernandez M.-P. Biochem. J. 337:125-131(1999) [PubMed: 9854034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ. Tissue: Liver.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Bone marrow.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary gland.
[6]	Lubec G., Klug S., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 28-43; 62-74; 116-124; 192-199; 226-240 AND 275-283, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain and Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U29396 mRNA. Translation: AAC52530.1. D63423 mRNA. Translation: BAA09728.1. AJ230108 , AJ230110, AJ230111, AJ230114, AJ230116, AJ230118, AJ230119, AJ230120, AJ230121, AJ230122, AJ230123, AJ230124 Genomic DNA. Translation: CAA13092.1. AK147740 mRNA. Translation: BAE28107.1. AK152185 mRNA. Translation: BAE31016.1. AK153388 mRNA. Translation: BAE31952.1. AK153476 mRNA. Translation: BAE32026.1. BC003716 mRNA. Translation: AAH03716.1.
IPI	IPI00317309.
RefSeq	NP_033803.1. NM_009673.2.
UniGene	Mm.1620.
3D structure databases
ProteinModelPortal	P48036.
SMR	P48036. Positions 2-319.
ModBase	Search...
Protein-protein interaction databases
IntAct	P48036. 4 interactions.
STRING	P48036.
PTM databases
PhosphoSite	P48036.
2D gel databases
SWISS-2DPAGE	P48036.
COMPLUYEAST-2DPAGE	P48036.
REPRODUCTION-2DPAGE	IPI00317309.
Proteomic databases
PRIDE	P48036.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000029266; ENSMUSP00000029266; ENSMUSG00000027712.
GeneID	11747.
KEGG	mmu:11747.
NMPDR	fig|10090.3.peg.7935.
UCSC	uc008ozj.2. mouse.
Organism-specific databases
CTD	308.
MGI	MGI:106008. Anxa5.
Phylogenomic databases
eggNOG	roNOG12109.
GeneTree	ENSGT00580000081227.
HOGENOM	HBG745057.
HOVERGEN	HBG061815.
InParanoid	P48036.
OMA	RGTVTAF.
OrthoDB	EOG4W9J4D.
PhylomeDB	P48036.
Gene expression databases
ArrayExpress	P48036.
Bgee	P48036.
CleanEx	MM_ANXA5.
Genevestigator	P48036.
GermOnline	ENSMUSG00000027712. Mus musculus.
Family and domain databases
InterPro	IPR001464. Annexin. IPR018502. Annexin_repeat. IPR018252. Annexin_repeat_CS. IPR015473. Annexins_V. IPR002392. AnnexinV. [Graphical view]
Gene3D	G3DSA:1.10.220.10. Annexin. 4 hits.
PANTHER	PTHR10502. Annexin. 1 hit. PTHR10502:SF26. Annexins_V. 1 hit.
Pfam	PF00191. Annexin. 4 hits. [Graphical view]
PRINTS	PR00196. ANNEXIN. PR00201. ANNEXINV.
SMART	SM00335. ANX. 4 hits. [Graphical view]
SUPFAM	SSF47874. Annexin. 1 hit.
PROSITE	PS00223. ANNEXIN. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	279485.
SOURCE	Search...

Entry information

Entry name

ANXA5_MOUSE

Accession

Primary (citable) accession number: P48036
Secondary accession number(s): Q3U5Q1 , Q3U5X4, Q3U8K1, Q3UGV0, Q99LA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	October 19, 2011
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program