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Protein

Metalloproteinase inhibitor 3

Gene

Timp3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi24 – 241Zinc; via amino nitrogen and carbonyl oxygen; shared with metalloproteinase partnerBy similarity

GO - Molecular functioni

GO - Biological processi

  • aging Source: RGD
  • central nervous system development Source: RGD
  • negative regulation of endopeptidase activity Source: GO_Central
  • negative regulation of membrane protein ectodomain proteolysis Source: GO_Central
  • negative regulation of metalloenzyme activity Source: GO_Central
  • negative regulation of proteolysis Source: RGD
  • response to amino acid Source: RGD
  • response to cytokine Source: GO_Central
  • response to estrogen Source: RGD
  • response to folic acid Source: RGD
  • response to hormone Source: RGD
  • response to mechanical stimulus Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to organic substance Source: RGD
  • tissue regeneration Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Metalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiI35.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Metalloproteinase inhibitor 3
Alternative name(s):
Tissue inhibitor of metalloproteinases 3
Short name:
TIMP-3
Gene namesi
Name:Timp3
Synonyms:Timp-3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3865. Timp3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • extracellular space Source: GO_Central
  • proteinaceous extracellular matrix Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 211188Metalloproteinase inhibitor 3PRO_0000034345Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi24 ↔ 91PROSITE-ProRule annotation
Disulfide bondi26 ↔ 118PROSITE-ProRule annotation
Disulfide bondi36 ↔ 143PROSITE-ProRule annotation
Disulfide bondi145 ↔ 192PROSITE-ProRule annotation
Disulfide bondi150 ↔ 155PROSITE-ProRule annotation
Disulfide bondi163 ↔ 184PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP48032.

Expressioni

Gene expression databases

GenevestigatoriP48032.

Interactioni

Subunit structurei

Interacts with EFEMP1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005746.

Structurei

3D structure databases

ProteinModelPortaliP48032.
SMRiP48032. Positions 24-144.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 143120NTRPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 285Involved in metalloproteinase-bindingBy similarity
Regioni88 – 892Involved in metalloproteinase-bindingBy similarity
Regioni105 – 18884Mediates interaction with EFEMP1By similarityAdd
BLAST

Sequence similaritiesi

Contains 1 NTR domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG250837.
HOGENOMiHOG000285981.
HOVERGENiHBG068749.
InParanoidiP48032.
PhylomeDBiP48032.

Family and domain databases

Gene3Di3.90.370.10. 1 hit.
InterProiIPR001134. Netrin_domain.
IPR001820. Prot_inh_TIMP.
IPR008993. TIMP-like_OB-fold.
IPR027465. TIMP_C_dom.
IPR030490. TIMP_CS.
IPR015612. Tissue_inhib_metalloprotease_3.
[Graphical view]
PANTHERiPTHR11844. PTHR11844. 1 hit.
PTHR11844:SF6. PTHR11844:SF6. 1 hit.
PfamiPF00965. TIMP. 1 hit.
[Graphical view]
SMARTiSM00206. NTR. 1 hit.
[Graphical view]
SUPFAMiSSF50242. SSF50242. 1 hit.
PROSITEiPS50189. NTR. 1 hit.
PS00288. TIMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48032-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPWLGLVVL LSCWSLGHWG TEACTCSPSH PQDAFCNSDI VIRAKVVGKK
60 70 80 90 100
LVKEGPFGTL VYTIKQMKMY RGFSKMPHVQ YIHTEASESL CGLKLEVNKY
110 120 130 140 150
QYLLTGRVYE GKMYTGLCNF VERWDHLTLS QRKGLNYRYH LGCNCKIKSC
160 170 180 190 200
YYLPCFVTSK KECLWTDMLS NFGYPGYQSK HYACIRQKGG YCSWYRGWAP
210
PDKSISNATD P
Length:211
Mass (Da):24,226
Last modified:February 1, 1996 - v1
Checksum:i142FD4EB4BA8791E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27201 mRNA. Translation: AAA75002.1.
PIRiJC4630.
UniGeneiRn.119634.

Genome annotation databases

UCSCiRGD:3865. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27201 mRNA. Translation: AAA75002.1.
PIRiJC4630.
UniGeneiRn.119634.

3D structure databases

ProteinModelPortaliP48032.
SMRiP48032. Positions 24-144.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005746.

Chemistry

BindingDBiP48032.
ChEMBLiCHEMBL3881.

Protein family/group databases

MEROPSiI35.003.

Proteomic databases

PaxDbiP48032.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:3865. rat.

Organism-specific databases

RGDi3865. Timp3.

Phylogenomic databases

eggNOGiNOG250837.
HOGENOMiHOG000285981.
HOVERGENiHBG068749.
InParanoidiP48032.
PhylomeDBiP48032.

Miscellaneous databases

PROiP48032.

Gene expression databases

GenevestigatoriP48032.

Family and domain databases

Gene3Di3.90.370.10. 1 hit.
InterProiIPR001134. Netrin_domain.
IPR001820. Prot_inh_TIMP.
IPR008993. TIMP-like_OB-fold.
IPR027465. TIMP_C_dom.
IPR030490. TIMP_CS.
IPR015612. Tissue_inhib_metalloprotease_3.
[Graphical view]
PANTHERiPTHR11844. PTHR11844. 1 hit.
PTHR11844:SF6. PTHR11844:SF6. 1 hit.
PfamiPF00965. TIMP. 1 hit.
[Graphical view]
SMARTiSM00206. NTR. 1 hit.
[Graphical view]
SUPFAMiSSF50242. SSF50242. 1 hit.
PROSITEiPS50189. NTR. 1 hit.
PS00288. TIMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of the cDNA encoding rat tissue inhibitor of metalloproteinase 3 (TIMP-3)."
    Wu I., Moses M.A.
    Gene 168:243-246(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiTIMP3_RAT
AccessioniPrimary (citable) accession number: P48032
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 1, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.