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P48032 (TIMP3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Metalloproteinase inhibitor 3
Alternative name(s):
Tissue inhibitor of metalloproteinases 3
Short name=TIMP-3
Gene names
Name:Timp3
Synonyms:Timp-3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length211 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli.

Subunit structure

Interacts with EFEMP1 By similarity.

Subcellular location

Secretedextracellular spaceextracellular matrix.

Sequence similarities

Belongs to the protease inhibitor I35 (TIMP) family. [View classification]

Contains 1 NTR domain.

Ontologies

Keywords
   Cellular componentExtracellular matrix
Secreted
   DomainSignal
   LigandMetal-binding
Zinc
   Molecular functionMetalloenzyme inhibitor
Metalloprotease inhibitor
Protease inhibitor
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaging

Inferred from expression pattern PubMed 18278151. Source: RGD

central nervous system development

Inferred from expression pattern PubMed 11044612. Source: RGD

negative regulation of proteolysis

Inferred from mutant phenotype PubMed 17532789. Source: RGD

response to amino acid

Inferred from expression pattern PubMed 17083784. Source: RGD

response to estrogen

Inferred from expression pattern PubMed 15866537. Source: RGD

response to folic acid

Inferred from expression pattern PubMed 18156304. Source: RGD

response to hormone

Inferred from expression pattern PubMed 17275272. Source: RGD

response to mechanical stimulus

Inferred from expression pattern PubMed 17717962. Source: RGD

response to organic cyclic compound

Inferred from expression pattern PubMed 15866537. Source: RGD

response to organic substance

Inferred from expression pattern PubMed 17555880. Source: RGD

tissue regeneration

Inferred from expression pattern PubMed 16683235. Source: RGD

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 17551674. Source: RGD

proteinaceous extracellular matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

metalloendopeptidase inhibitor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 211188Metalloproteinase inhibitor 3
PRO_0000034345

Regions

Domain24 – 143120NTR
Region24 – 285Involved in metalloproteinase-binding By similarity
Region88 – 892Involved in metalloproteinase-binding By similarity
Region105 – 18884Mediates interaction with EFEMP1 By similarity

Sites

Metal binding241Zinc; via amino nitrogen and carbonyl oxygen; shared with metalloproteinase partner By similarity

Amino acid modifications

Disulfide bond24 ↔ 91 By similarity
Disulfide bond26 ↔ 118 By similarity
Disulfide bond36 ↔ 143 By similarity
Disulfide bond145 ↔ 192 By similarity
Disulfide bond150 ↔ 155 By similarity
Disulfide bond163 ↔ 184 By similarity

Sequences

Sequence LengthMass (Da)Tools
P48032 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 142FD4EB4BA8791E

FASTA21124,226
        10         20         30         40         50         60 
MTPWLGLVVL LSCWSLGHWG TEACTCSPSH PQDAFCNSDI VIRAKVVGKK LVKEGPFGTL 

        70         80         90        100        110        120 
VYTIKQMKMY RGFSKMPHVQ YIHTEASESL CGLKLEVNKY QYLLTGRVYE GKMYTGLCNF 

       130        140        150        160        170        180 
VERWDHLTLS QRKGLNYRYH LGCNCKIKSC YYLPCFVTSK KECLWTDMLS NFGYPGYQSK 

       190        200        210 
HYACIRQKGG YCSWYRGWAP PDKSISNATD P 

« Hide

References

[1]"Cloning and expression of the cDNA encoding rat tissue inhibitor of metalloproteinase 3 (TIMP-3)."
Wu I., Moses M.A.
Gene 168:243-246(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U27201 mRNA. Translation: AAA75002.1.
PIRJC4630.
UniGeneRn.119634.

3D structure databases

ProteinModelPortalP48032.
SMRP48032. Positions 24-144.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000005746.

Chemistry

BindingDBP48032.
ChEMBLCHEMBL3881.

Protein family/group databases

MEROPSI35.003.

Proteomic databases

PaxDbP48032.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:3865. rat.

Organism-specific databases

RGD3865. Timp3.

Phylogenomic databases

eggNOGNOG250837.
HOGENOMHOG000285981.
HOVERGENHBG068749.
InParanoidP48032.
PhylomeDBP48032.

Gene expression databases

GenevestigatorP48032.

Family and domain databases

Gene3D3.90.370.10. 1 hit.
InterProIPR001134. Netrin_domain.
IPR001820. Prot_inh_TIMP.
IPR008993. TIMP-like_OB-fold.
IPR027465. TIMP_C_dom.
IPR015612. Tissue_inhib_metalloprotease_3.
[Graphical view]
PANTHERPTHR11844. PTHR11844. 1 hit.
PTHR11844:SF6. PTHR11844:SF6. 1 hit.
PfamPF00965. TIMP. 1 hit.
[Graphical view]
SMARTSM00206. NTR. 1 hit.
[Graphical view]
SUPFAMSSF50242. SSF50242. 1 hit.
PROSITEPS50189. NTR. 1 hit.
PS00288. TIMP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP48032.

Entry information

Entry nameTIMP3_RAT
AccessionPrimary (citable) accession number: P48032
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families