P48031 (GBX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeobox protein GBX-2

Alternative name(s):
Gastrulation and brain-specific homeobox protein 2
Stimulated by retinoic acid gene 7 protein

Gene names

Name:	Gbx2
Synonyms:	Mmoxa, Stra7

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May act as a transcription factor for cell pluripotency and differentiation in the embryo.
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed in adult brain, spleen and female genital tract. No expression in heart, liver, lung, kidney, or testis.
Developmental stage	Expressed during gastrulation in the three primitive germ layers and in the pharyngeal arches. In later stages, expressed in the developing nervous system, midbrain/ hindbrain boundary, cerebellum anlage, certain rhombomeres, regions of the spinal cord, and in the developing dorsal thalamus and corpus striatum.
Sequence similarities	Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autonomic nervous system development Inferred from mutant phenotype PubMed 15996652. Source: MGI axon guidance Inferred from mutant phenotype PubMed 11967891. Source: MGI cerebellar granule cell precursor proliferation Inferred from mutant phenotype PubMed 16651541. Source: MGI cerebellum development Inferred from mutant phenotype PubMed 16651541. Source: MGI forebrain neuron development Inferred from mutant phenotype PubMed 21048141. Source: MGI inner ear morphogenesis Inferred from mutant phenotype PubMed 15829521PubMed 15996652. Source: MGI midbrain-hindbrain boundary morphogenesis Inferred from mutant phenotype PubMed 16651541. Source: MGI neural crest cell migration Inferred from mutant phenotype PubMed 15996652PubMed 19700621. Source: MGI patterning of blood vessels Inferred from mutant phenotype PubMed 15996652. Source: MGI rhombomere 2 development Inferred from mutant phenotype PubMed 16651541. Source: MGI thalamus development Inferred from mutant phenotype PubMed 19279136. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Homeobox protein GBX-2		PRO_0000048881
Regions
	DNA binding	247 – 306	60	Homeobox
	Compositional bias	56 – 63	8	Poly-Pro
	Compositional bias	121 – 124	4	Poly-Ala
	Compositional bias	248 – 251	4	Poly-Arg

Sequences

	Sequence		Length	Mass (Da)
	P48031 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: CF8594653EA8295B	FASTA	348	37,359
	10 20 30 40 50 60 MSAAFPPSLM MMQRPLGSST AFSIDSLIGS PPQPSPGHFV YTGYPMFMPY RPVVLPPPPP 70 80 90 100 110 120 PPPALPQAAL QPALPPAHPH HQIPSLPTGF CSSLAQGMAL TSTLMATLPG GFSASPQHQE 130 140 150 160 170 180 AAAARKFAPQ PLPGGGNFDK AEALQADAED GKAFLAKEGS LLAFSAAEAV QASLVGAVRG 190 200 210 220 230 240 QGKDESKVED DPKGKEESFS LESDVDYSSD DNLPGQTAHK EEDPGHALEE TPQSGGAAGS 250 260 270 280 290 300 TTSTGKNRRR RTAFTSEQLL ELEKEFHCKK YLSLTERSQI AHALKLSEVQ VKIWFQNRRA 310 320 330 340 KWKRVKAGNA NSKTGEPSRN PKIVVPIPVH VSRFAIRSQH QQLEQARP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and expression pattern of the Stra7 (Gbx-2) homeobox-containing gene induced by retinoic acid in P19 embryonal carcinoma cells." Bouillet P., Chazaud C., Oulad Abdelghani M., Dolle P., Chambon P. Dev. Dyn. 204:372-382(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The mouse homeobox gene, Gbx2: genomic organization and expression in pluripotent cells in vitro and in vivo." Chapman G., Remiszewski J.L., Webb G.C., Schulz T.C., Bottema C.D.K., Rathjen P.D. Genomics 46:223-233(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Sequence and evolutionary conservation of the murine Gbx-2 homeobox gene." Chapman G., Rathjen P.D. FEBS Lett. 364:289-292(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-348. Strain: 129.
[5]	"Detection of homeobox genes in development and evolution." Murtha M.T., Leckman J.F., Ruddle F.H. Proc. Natl. Acad. Sci. U.S.A. 88:10711-10715(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 268-292. Strain: C57BL/6. Tissue: Spleen.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48800 mRNA. Translation: CAA88737.1. U74300 Genomic DNA. Translation: AAC61877.1. BC116965 mRNA. Translation: AAI16966.1. BC120492 mRNA. Translation: AAI20493.1. L39770 mRNA. Translation: AAA65185.1. M81661 Genomic DNA. Translation: AAA63314.1.
IPI	IPI00137999.
PIR	S52720.
RefSeq	NP_034392.1. NM_010262.3.
UniGene	Mm.204730.
3D structure databases
ProteinModelPortal	P48031.
SMR	P48031. Positions 251-304.
ModBase	Search...
PTM databases
PhosphoSite	P48031.
Proteomic databases
PRIDE	P48031.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000036954; ENSMUSP00000048508; ENSMUSG00000034486.
GeneID	14472.
KEGG	mmu:14472.
UCSC	uc007bzb.1. mouse.
Organism-specific databases
CTD	2637.
MGI	MGI:95668. Gbx2.
Phylogenomic databases
eggNOG	NOG315879.
GeneTree	ENSGT00660000095141.
HOGENOM	HOG000060099.
HOVERGEN	HBG003967.
InParanoid	Q14A66.
KO	K09321.
OMA	HQEAARK.
OrthoDB	EOG4N5VXB.
Gene expression databases
ArrayExpress	P48031.
Bgee	P48031.
CleanEx	MM_GBX2.
Genevestigator	P48031.
GermOnline	ENSMUSG00000034486. Mus musculus.
Family and domain databases
Gene3D	1.10.10.60. 1 hit.
InterPro	IPR017970. Homeobox_CS. IPR020479. Homeobox_metazoa. IPR001356. Homeodomain. IPR009057. Homeodomain-like. [Graphical view]
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00024. HOMEOBOX.
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	286138.
SOURCE	Search...

Entry information

Entry name

GBX2_MOUSE

Accession

Primary (citable) accession number: P48031
Secondary accession number(s): Q14A66

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program