P48030 (TGFA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protransforming growth factor alpha

Cleaved into the following chain:

1. Transforming growth factor alpha
   Short name=TGF-alpha
   Alternative name(s):
   EGF-like TGF
   Short name=ETGF
   TGF type 1

Gene names

Name:

Tgfa

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	159 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Subunit structure	Interacts with the PDZ domains of SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2 By similarity. Interacts with MAGI3. Ref.3
Subcellular location	Transforming growth factor alpha: Secreted › extracellular space. Protransforming growth factor alpha: Cell membrane; Single-pass type I membrane protein.
Sequence similarities	Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Domain	EGF-like domain Signal Transmembrane Transmembrane helix
Molecular function	Growth factor Mitogen
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	angiogenesis Inferred from direct assay PubMed 14998491. Source: MGI epidermal growth factor receptor signaling pathway Inferred from direct assay PubMed 6315706. Source: MGI mammary gland alveolus development Inferred from genetic interaction PubMed 10331984. Source: MGI negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of cell division Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of epidermal growth factor-activated receptor activity Inferred from sequence or structural similarity. Source: HGNC positive regulation of epithelial cell proliferation Inferred from sequence or structural similarity. Source: HGNC positive regulation of mitosis Inferred from sequence or structural similarity. Source: HGNC response to drug Inferred from electronic annotation. Source: Compara wound healing Inferred from electronic annotation. Source: Compara
Cellular_component	basolateral plasma membrane Inferred from electronic annotation. Source: Compara cell surface Inferred from electronic annotation. Source: Compara cytoplasmic vesicle Inferred from electronic annotation. Source: Compara extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: Compara perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara
Molecular_function	MAP kinase kinase activity Inferred from sequence or structural similarity. Source: HGNC epidermal growth factor receptor binding Inferred from sequence or structural similarity. Source: UniProtKB glycoprotein binding Inferred from direct assay PubMed 15572151. Source: MGI growth factor activity Inferred from sequence or structural similarity. Source: HGNC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 159	136	Protransforming growth factor alpha		PRO_0000302746
	Propeptide	24 – 38	15	Removed in mature form		PRO_0000007758
	Chain	39 – 88	50	Transforming growth factor alpha		PRO_0000007759
	Propeptide	89 – 159	71	Removed in mature form		PRO_0000007760
Regions
	Topological domain	24 – 97	74	Extracellular Potential
	Transmembrane	98 – 123	26	Helical; Potential
	Topological domain	124 – 159	36	Cytoplasmic Potential
	Domain	44 – 83	40	EGF-like
Amino acid modifications
	Lipidation	152	1	S-palmitoyl cysteine By similarity
	Lipidation	153	1	S-palmitoyl cysteine By similarity
	Glycosylation	25	1	N-linked (GlcNAc...) Potential
	Disulfide bond	46 ↔ 59		By similarity
	Disulfide bond	54 ↔ 70		By similarity
	Disulfide bond	72 ↔ 81		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48030 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: BE0AE8D89CE7DDFD	FASTA	159	17,018
	10 20 30 40 50 60 MVPATGQLAL LALGILLAVC QALENSTSPL SDSPVAAAVV SHFNKCPDSH TQYCFHGTCR 70 80 90 100 110 120 FLVQEEKPAC VCHSGYVGVR CEHADLLAVV AASQKKQAIT ALVVVSIVAL AVLIITCVLI 130 140 150 HCCQLRKHCE WCRALVCRHE KPSALLKGRT ACCHSETVV

References

[1]	"Nucleotide sequence and tissue distribution of mouse transforming growth factor-alpha." Vaughan T.J., Pascall J.C., Brown K.D. Biochim. Biophys. Acta 1132:322-324(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization of the mouse transforming growth factor alpha gene: its expression during eyelid development and in waved 1 tissues." Berkowitz E.A., Seroogy K.B., Schroeder J.A., Russell W.E., Evans E.P., Riedel R.F., Phillips H.K., Harrison C.A., Lee D.C., Luetteke N.C. Cell Growth Differ. 7:1271-1282(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: 129/Sv. Tissue: Brain.
[3]	"Identification of MAGI-3 as a transforming growth factor-alpha tail binding protein." Franklin J.L., Yoshiura K., Dempsey P.J., Bogatcheva G., Jeyakumar L., Meise K.S., Pearsall R.S., Threadgill D., Coffey R.J. Exp. Cell Res. 303:457-470(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MAGI3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M92420 mRNA. Translation: AAA40481.1. U65016 mRNA. Translation: AAB50554.1. U64873 Genomic DNA. Translation: AAB50553.1.
IPI	IPI00137996.
PIR	S27195.
RefSeq	NP_112476.1. NM_031199.3.
UniGene	Mm.137222.
3D structure databases
ProteinModelPortal	P48030.
SMR	P48030. Positions 39-88.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1894871.
STRING	10090.ENSMUSP00000032066.
Proteomic databases
PaxDb	P48030.
PRIDE	P48030.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032066; ENSMUSP00000032066; ENSMUSG00000029999.
GeneID	21802.
KEGG	mmu:21802.
Organism-specific databases
CTD	7039.
MGI	MGI:98724. Tgfa.
Phylogenomic databases
eggNOG	NOG41326.
HOGENOM	HOG000013036.
HOVERGEN	HBG000330.
InParanoid	P48030.
KO	K08774.
OMA	IGARCEH.
OrthoDB	EOG4SN1PX.
Gene expression databases
ArrayExpress	P48030.
Bgee	P48030.
CleanEx	MM_TGFA.
Genevestigator	P48030.
GermOnline	ENSMUSG00000029999. Mus musculus.
Family and domain databases
InterPro	IPR000742. EG-like_dom. IPR013032. EGF-like_CS. IPR015497. EGF_rcpt_ligand. [Graphical view]
PANTHER	PTHR10740. PTHR10740. 1 hit.
SMART	SM00181. EGF. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TGFA. mouse.
NextBio	301172.
PMAP-CutDB	P48030.
SOURCE	Search...

Entry information

Entry name

TGFA_MOUSE

Accession

Primary (citable) accession number: P48030

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program