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P48030 (TGFA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protransforming growth factor alpha

Cleaved into the following chain:

  1. Transforming growth factor alpha
    Short name=TGF-alpha
    Alternative name(s):
    EGF-like TGF
    Short name=ETGF
    TGF type 1
Gene names
Name:Tgfa
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length159 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Subunit structure

Interacts with the PDZ domains of SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2 By similarity. Interacts with MAGI3. Ref.3

Subcellular location

Transforming growth factor alpha: Secretedextracellular space.

Protransforming growth factor alpha: Cell membrane; Single-pass type I membrane protein.

Sequence similarities

Contains 1 EGF-like domain.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
Secreted
   DomainEGF-like domain
Signal
Transmembrane
Transmembrane helix
   Molecular functionGrowth factor
Mitogen
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of MAPK activity

Inferred from sequence or structural similarity. Source: HGNC

angiogenesis

Inferred from direct assay PubMed 14998491. Source: MGI

epidermal growth factor receptor signaling pathway

Inferred from direct assay PubMed 6315706. Source: MGI

mammary gland alveolus development

Inferred from genetic interaction PubMed 10331984. Source: MGI

negative regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell division

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of epidermal growth factor-activated receptor activity

Inferred from sequence or structural similarity. Source: HGNC

positive regulation of epithelial cell proliferation

Inferred from sequence or structural similarity. Source: HGNC

positive regulation of mitosis

Inferred from sequence or structural similarity. Source: HGNC

response to drug

Inferred from electronic annotation. Source: Ensembl

wound healing

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentbasolateral plasma membrane

Inferred from electronic annotation. Source: Ensembl

cell surface

Inferred from electronic annotation. Source: Ensembl

cytoplasmic vesicle

Inferred from electronic annotation. Source: Ensembl

extracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

nucleus

Inferred from electronic annotation. Source: Ensembl

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionMAP kinase kinase activity

Inferred from sequence or structural similarity. Source: HGNC

epidermal growth factor receptor binding

Inferred from sequence or structural similarity. Source: UniProtKB

glycoprotein binding

Inferred from direct assay PubMed 15572151. Source: MGI

growth factor activity

Inferred from sequence or structural similarity. Source: HGNC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 159136Protransforming growth factor alpha
PRO_0000302746
Propeptide24 – 3815Removed in mature form
PRO_0000007758
Chain39 – 8850Transforming growth factor alpha
PRO_0000007759
Propeptide89 – 15971Removed in mature form
PRO_0000007760

Regions

Topological domain24 – 9774Extracellular Potential
Transmembrane98 – 12326Helical; Potential
Topological domain124 – 15936Cytoplasmic Potential
Domain44 – 8340EGF-like

Amino acid modifications

Lipidation1521S-palmitoyl cysteine By similarity
Lipidation1531S-palmitoyl cysteine By similarity
Glycosylation251N-linked (GlcNAc...) Potential
Disulfide bond46 ↔ 59 By similarity
Disulfide bond54 ↔ 70 By similarity
Disulfide bond72 ↔ 81 By similarity

Sequences

Sequence LengthMass (Da)Tools
P48030 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: BE0AE8D89CE7DDFD

FASTA15917,018
        10         20         30         40         50         60 
MVPATGQLAL LALGILLAVC QALENSTSPL SDSPVAAAVV SHFNKCPDSH TQYCFHGTCR 

        70         80         90        100        110        120 
FLVQEEKPAC VCHSGYVGVR CEHADLLAVV AASQKKQAIT ALVVVSIVAL AVLIITCVLI 

       130        140        150 
HCCQLRKHCE WCRALVCRHE KPSALLKGRT ACCHSETVV 

« Hide

References

[1]"Nucleotide sequence and tissue distribution of mouse transforming growth factor-alpha."
Vaughan T.J., Pascall J.C., Brown K.D.
Biochim. Biophys. Acta 1132:322-324(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Characterization of the mouse transforming growth factor alpha gene: its expression during eyelid development and in waved 1 tissues."
Berkowitz E.A., Seroogy K.B., Schroeder J.A., Russell W.E., Evans E.P., Riedel R.F., Phillips H.K., Harrison C.A., Lee D.C., Luetteke N.C.
Cell Growth Differ. 7:1271-1282(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: 129/Sv.
Tissue: Brain.
[3]"Identification of MAGI-3 as a transforming growth factor-alpha tail binding protein."
Franklin J.L., Yoshiura K., Dempsey P.J., Bogatcheva G., Jeyakumar L., Meise K.S., Pearsall R.S., Threadgill D., Coffey R.J.
Exp. Cell Res. 303:457-470(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MAGI3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M92420 mRNA. Translation: AAA40481.1.
U65016 mRNA. Translation: AAB50554.1.
U64873 Genomic DNA. Translation: AAB50553.1.
PIRS27195.
RefSeqNP_112476.1. NM_031199.3.
UniGeneMm.137222.

3D structure databases

ProteinModelPortalP48030.
SMRP48030. Positions 39-88.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP48030. 1 interaction.
MINTMINT-1894871.
STRING10090.ENSMUSP00000032066.

Proteomic databases

PaxDbP48030.
PRIDEP48030.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032066; ENSMUSP00000032066; ENSMUSG00000029999.
GeneID21802.
KEGGmmu:21802.
UCSCuc009crk.2. mouse.

Organism-specific databases

CTD7039.
MGIMGI:98724. Tgfa.

Phylogenomic databases

eggNOGNOG41326.
HOGENOMHOG000013036.
HOVERGENHBG000330.
InParanoidP48030.
KOK08774.
OMACHSETGC.
OrthoDBEOG7VQJGP.
PhylomeDBP48030.
TreeFamTF332938.

Gene expression databases

ArrayExpressP48030.
BgeeP48030.
CleanExMM_TGFA.
GenevestigatorP48030.

Family and domain databases

InterProIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR015497. EGF_rcpt_ligand.
[Graphical view]
PANTHERPTHR10740. PTHR10740. 1 hit.
SMARTSM00181. EGF. 1 hit.
[Graphical view]
PROSITEPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTGFA. mouse.
NextBio301172.
PMAP-CutDBP48030.
PROP48030.
SOURCESearch...

Entry information

Entry nameTGFA_MOUSE
AccessionPrimary (citable) accession number: P48030
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot