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Protein

Protransforming growth factor alpha

Gene

Tgfa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

GO - Molecular functioni

  • epidermal growth factor receptor binding Source: MGI
  • growth factor activity Source: HGNC

GO - Biological processi

Keywordsi

Molecular functionGrowth factor, Mitogen

Enzyme and pathway databases

ReactomeiR-MMU-204005 COPII (Coat Protein 2) Mediated Vesicle Transport
R-MMU-5694530 Cargo concentration in the ER

Names & Taxonomyi

Protein namesi
Recommended name:
Protransforming growth factor alpha
Cleaved into the following chain:
Alternative name(s):
EGF-like TGF
Short name:
ETGF
TGF type 1
Gene namesi
Name:Tgfa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:98724 Tgfa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 97ExtracellularSequence analysisAdd BLAST74
Transmembranei98 – 123HelicalSequence analysisAdd BLAST26
Topological domaini124 – 159CytoplasmicSequence analysisAdd BLAST36

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000030274624 – 159Protransforming growth factor alphaAdd BLAST136
PropeptideiPRO_000000775824 – 38Removed in mature formAdd BLAST15
ChainiPRO_000000775939 – 88Transforming growth factor alphaAdd BLAST50
PropeptideiPRO_000000776089 – 159Removed in mature formAdd BLAST71

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi46 ↔ 59PROSITE-ProRule annotation
Disulfide bondi54 ↔ 70PROSITE-ProRule annotation
Disulfide bondi72 ↔ 81PROSITE-ProRule annotation
Lipidationi152S-palmitoyl cysteineBy similarity1
Lipidationi153S-palmitoyl cysteineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP48030
PRIDEiP48030

Miscellaneous databases

PMAP-CutDBiP48030

Expressioni

Gene expression databases

BgeeiENSMUSG00000029999
CleanExiMM_TGFA
ExpressionAtlasiP48030 baseline and differential
GenevisibleiP48030 MM

Interactioni

Subunit structurei

Interacts with the PDZ domains of SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2 (By similarity). Interacts with MAGI3.By similarity1 Publication

GO - Molecular functioni

  • epidermal growth factor receptor binding Source: MGI
  • growth factor activity Source: HGNC

Protein-protein interaction databases

IntActiP48030, 1 interactor
MINTiP48030
STRINGi10090.ENSMUSP00000032066

Structurei

3D structure databases

ProteinModelPortaliP48030
SMRiP48030
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 83EGF-likePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IVW5 Eukaryota
ENOG4111Z4I LUCA
GeneTreeiENSGT00730000110951
HOGENOMiHOG000013036
HOVERGENiHBG000330
InParanoidiP48030
KOiK08774
OMAiLIHCCEV
OrthoDBiEOG091G0RK4
PhylomeDBiP48030
TreeFamiTF332938

Family and domain databases

InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR015497 EGF_rcpt_ligand
PANTHERiPTHR10740 PTHR10740, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48030-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPATGQLAL LALGILLAVC QALENSTSPL SDSPVAAAVV SHFNKCPDSH
60 70 80 90 100
TQYCFHGTCR FLVQEEKPAC VCHSGYVGVR CEHADLLAVV AASQKKQAIT
110 120 130 140 150
ALVVVSIVAL AVLIITCVLI HCCQLRKHCE WCRALVCRHE KPSALLKGRT

ACCHSETVV
Length:159
Mass (Da):17,018
Last modified:February 1, 1996 - v1
Checksum:iBE0AE8D89CE7DDFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92420 mRNA Translation: AAA40481.1
U65016 mRNA Translation: AAB50554.1
U64873 Genomic DNA Translation: AAB50553.1
CCDSiCCDS51832.1
PIRiS27195
RefSeqiNP_112476.1, NM_031199.4
UniGeneiMm.137222

Genome annotation databases

EnsembliENSMUST00000032066; ENSMUSP00000032066; ENSMUSG00000029999
GeneIDi21802
KEGGimmu:21802
UCSCiuc009crk.2 mouse

Similar proteinsi

Entry informationi

Entry nameiTGFA_MOUSE
AccessioniPrimary (citable) accession number: P48030
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 28, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health