Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protransforming growth factor alpha

Gene

Tgfa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

GO - Molecular functioni

  • epidermal growth factor receptor binding Source: MGI
  • growth factor activity Source: HGNC

GO - Biological processi

Keywordsi

Molecular functionGrowth factor, Mitogen

Enzyme and pathway databases

ReactomeiR-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-5694530. Cargo concentration in the ER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protransforming growth factor alpha
Cleaved into the following chain:
Alternative name(s):
EGF-like TGF
Short name:
ETGF
TGF type 1
Gene namesi
Name:Tgfa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:98724. Tgfa.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 97ExtracellularSequence analysisAdd BLAST74
Transmembranei98 – 123HelicalSequence analysisAdd BLAST26
Topological domaini124 – 159CytoplasmicSequence analysisAdd BLAST36

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000030274624 – 159Protransforming growth factor alphaAdd BLAST136
PropeptideiPRO_000000775824 – 38Removed in mature formAdd BLAST15
ChainiPRO_000000775939 – 88Transforming growth factor alphaAdd BLAST50
PropeptideiPRO_000000776089 – 159Removed in mature formAdd BLAST71

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi46 ↔ 59PROSITE-ProRule annotation
Disulfide bondi54 ↔ 70PROSITE-ProRule annotation
Disulfide bondi72 ↔ 81PROSITE-ProRule annotation
Lipidationi152S-palmitoyl cysteineBy similarity1
Lipidationi153S-palmitoyl cysteineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP48030.
PRIDEiP48030.

Miscellaneous databases

PMAP-CutDBiP48030.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029999.
CleanExiMM_TGFA.
ExpressionAtlasiP48030. baseline and differential.
GenevisibleiP48030. MM.

Interactioni

Subunit structurei

Interacts with the PDZ domains of SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2 (By similarity). Interacts with MAGI3.By similarity1 Publication

GO - Molecular functioni

  • epidermal growth factor receptor binding Source: MGI
  • growth factor activity Source: HGNC

Protein-protein interaction databases

IntActiP48030. 1 interactor.
MINTiMINT-1894871.
STRINGi10090.ENSMUSP00000032066.

Structurei

3D structure databases

ProteinModelPortaliP48030.
SMRiP48030.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 83EGF-likePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IVW5. Eukaryota.
ENOG4111Z4I. LUCA.
GeneTreeiENSGT00730000110951.
HOGENOMiHOG000013036.
HOVERGENiHBG000330.
InParanoidiP48030.
KOiK08774.
OMAiLIHCCEV.
OrthoDBiEOG091G0RK4.
PhylomeDBiP48030.
TreeFamiTF332938.

Family and domain databases

InterProiView protein in InterPro
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR015497. EGF_rcpt_ligand.
PANTHERiPTHR10740. PTHR10740. 1 hit.
PROSITEiView protein in PROSITE
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48030-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPATGQLAL LALGILLAVC QALENSTSPL SDSPVAAAVV SHFNKCPDSH
60 70 80 90 100
TQYCFHGTCR FLVQEEKPAC VCHSGYVGVR CEHADLLAVV AASQKKQAIT
110 120 130 140 150
ALVVVSIVAL AVLIITCVLI HCCQLRKHCE WCRALVCRHE KPSALLKGRT

ACCHSETVV
Length:159
Mass (Da):17,018
Last modified:February 1, 1996 - v1
Checksum:iBE0AE8D89CE7DDFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92420 mRNA. Translation: AAA40481.1.
U65016 mRNA. Translation: AAB50554.1.
U64873 Genomic DNA. Translation: AAB50553.1.
CCDSiCCDS51832.1.
PIRiS27195.
RefSeqiNP_112476.1. NM_031199.4.
UniGeneiMm.137222.

Genome annotation databases

EnsembliENSMUST00000032066; ENSMUSP00000032066; ENSMUSG00000029999.
GeneIDi21802.
KEGGimmu:21802.
UCSCiuc009crk.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiTGFA_MOUSE
AccessioniPrimary (citable) accession number: P48030
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 27, 2017
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot