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P48030

- TGFA_MOUSE

UniProt

P48030 - TGFA_MOUSE

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Protein

Protransforming growth factor alpha

Gene

Tgfa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

GO - Molecular functioni

  1. epidermal growth factor receptor binding Source: UniProtKB
  2. glycoprotein binding Source: MGI
  3. growth factor activity Source: HGNC
  4. MAP kinase kinase activity Source: HGNC

GO - Biological processi

  1. activation of MAPK activity Source: HGNC
  2. angiogenesis Source: MGI
  3. epidermal growth factor receptor signaling pathway Source: MGI
  4. mammary gland alveolus development Source: MGI
  5. negative regulation of apoptotic process Source: Ensembl
  6. positive regulation of cell division Source: UniProtKB-KW
  7. positive regulation of epidermal growth factor-activated receptor activity Source: HGNC
  8. positive regulation of epithelial cell proliferation Source: HGNC
  9. positive regulation of mitosis Source: HGNC
  10. response to drug Source: Ensembl
  11. wound healing Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Mitogen

Names & Taxonomyi

Protein namesi
Recommended name:
Protransforming growth factor alpha
Cleaved into the following chain:
Alternative name(s):
EGF-like TGF
Short name:
ETGF
TGF type 1
Gene namesi
Name:Tgfa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:98724. Tgfa.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 9774ExtracellularSequence AnalysisAdd
BLAST
Transmembranei98 – 12326HelicalSequence AnalysisAdd
BLAST
Topological domaini124 – 15936CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: Ensembl
  2. cell surface Source: Ensembl
  3. cytoplasmic vesicle Source: Ensembl
  4. extracellular space Source: Ensembl
  5. integral component of membrane Source: UniProtKB-KW
  6. nucleus Source: Ensembl
  7. perinuclear region of cytoplasm Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 159136Protransforming growth factor alphaPRO_0000302746Add
BLAST
Propeptidei24 – 3815Removed in mature formPRO_0000007758Add
BLAST
Chaini39 – 8850Transforming growth factor alphaPRO_0000007759Add
BLAST
Propeptidei89 – 15971Removed in mature formPRO_0000007760Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi25 – 251N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi46 ↔ 59PROSITE-ProRule annotation
Disulfide bondi54 ↔ 70PROSITE-ProRule annotation
Disulfide bondi72 ↔ 81PROSITE-ProRule annotation
Lipidationi152 – 1521S-palmitoyl cysteineBy similarity
Lipidationi153 – 1531S-palmitoyl cysteineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP48030.
PRIDEiP48030.

Miscellaneous databases

PMAP-CutDBP48030.

Expressioni

Gene expression databases

BgeeiP48030.
CleanExiMM_TGFA.
ExpressionAtlasiP48030. baseline and differential.
GenevestigatoriP48030.

Interactioni

Subunit structurei

Interacts with the PDZ domains of SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2 (By similarity). Interacts with MAGI3.By similarity1 Publication

Protein-protein interaction databases

IntActiP48030. 1 interaction.
MINTiMINT-1894871.
STRINGi10090.ENSMUSP00000032066.

Structurei

3D structure databases

ProteinModelPortaliP48030.
SMRiP48030. Positions 39-88.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 8340EGF-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 EGF-like domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG41326.
HOGENOMiHOG000013036.
HOVERGENiHBG000330.
InParanoidiP48030.
KOiK08774.
OMAiCHSETGC.
OrthoDBiEOG7VQJGP.
PhylomeDBiP48030.
TreeFamiTF332938.

Family and domain databases

InterProiIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR015497. EGF_rcpt_ligand.
[Graphical view]
PANTHERiPTHR10740. PTHR10740. 1 hit.
SMARTiSM00181. EGF. 1 hit.
[Graphical view]
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48030-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVPATGQLAL LALGILLAVC QALENSTSPL SDSPVAAAVV SHFNKCPDSH
60 70 80 90 100
TQYCFHGTCR FLVQEEKPAC VCHSGYVGVR CEHADLLAVV AASQKKQAIT
110 120 130 140 150
ALVVVSIVAL AVLIITCVLI HCCQLRKHCE WCRALVCRHE KPSALLKGRT

ACCHSETVV
Length:159
Mass (Da):17,018
Last modified:February 1, 1996 - v1
Checksum:iBE0AE8D89CE7DDFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92420 mRNA. Translation: AAA40481.1.
U65016 mRNA. Translation: AAB50554.1.
U64873 Genomic DNA. Translation: AAB50553.1.
CCDSiCCDS51832.1.
PIRiS27195.
RefSeqiNP_112476.1. NM_031199.3.
UniGeneiMm.137222.

Genome annotation databases

EnsembliENSMUST00000032066; ENSMUSP00000032066; ENSMUSG00000029999.
GeneIDi21802.
KEGGimmu:21802.
UCSCiuc009crk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92420 mRNA. Translation: AAA40481.1 .
U65016 mRNA. Translation: AAB50554.1 .
U64873 Genomic DNA. Translation: AAB50553.1 .
CCDSi CCDS51832.1.
PIRi S27195.
RefSeqi NP_112476.1. NM_031199.3.
UniGenei Mm.137222.

3D structure databases

ProteinModelPortali P48030.
SMRi P48030. Positions 39-88.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P48030. 1 interaction.
MINTi MINT-1894871.
STRINGi 10090.ENSMUSP00000032066.

Proteomic databases

PaxDbi P48030.
PRIDEi P48030.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000032066 ; ENSMUSP00000032066 ; ENSMUSG00000029999 .
GeneIDi 21802.
KEGGi mmu:21802.
UCSCi uc009crk.2. mouse.

Organism-specific databases

CTDi 7039.
MGIi MGI:98724. Tgfa.

Phylogenomic databases

eggNOGi NOG41326.
HOGENOMi HOG000013036.
HOVERGENi HBG000330.
InParanoidi P48030.
KOi K08774.
OMAi CHSETGC.
OrthoDBi EOG7VQJGP.
PhylomeDBi P48030.
TreeFami TF332938.

Miscellaneous databases

NextBioi 301172.
PMAP-CutDB P48030.
PROi P48030.
SOURCEi Search...

Gene expression databases

Bgeei P48030.
CleanExi MM_TGFA.
ExpressionAtlasi P48030. baseline and differential.
Genevestigatori P48030.

Family and domain databases

InterProi IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR015497. EGF_rcpt_ligand.
[Graphical view ]
PANTHERi PTHR10740. PTHR10740. 1 hit.
SMARTi SM00181. EGF. 1 hit.
[Graphical view ]
PROSITEi PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence and tissue distribution of mouse transforming growth factor-alpha."
    Vaughan T.J., Pascall J.C., Brown K.D.
    Biochim. Biophys. Acta 1132:322-324(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Characterization of the mouse transforming growth factor alpha gene: its expression during eyelid development and in waved 1 tissues."
    Berkowitz E.A., Seroogy K.B., Schroeder J.A., Russell W.E., Evans E.P., Riedel R.F., Phillips H.K., Harrison C.A., Lee D.C., Luetteke N.C.
    Cell Growth Differ. 7:1271-1282(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/Sv.
    Tissue: Brain.
  3. "Identification of MAGI-3 as a transforming growth factor-alpha tail binding protein."
    Franklin J.L., Yoshiura K., Dempsey P.J., Bogatcheva G., Jeyakumar L., Meise K.S., Pearsall R.S., Threadgill D., Coffey R.J.
    Exp. Cell Res. 303:457-470(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAGI3.

Entry informationi

Entry nameiTGFA_MOUSE
AccessioniPrimary (citable) accession number: P48030
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 26, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3