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P48030

- TGFA_MOUSE

UniProt

P48030 - TGFA_MOUSE

Protein

Protransforming growth factor alpha

Gene

Tgfa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

    GO - Molecular functioni

    1. epidermal growth factor receptor binding Source: UniProtKB
    2. glycoprotein binding Source: MGI
    3. growth factor activity Source: HGNC
    4. MAP kinase kinase activity Source: HGNC

    GO - Biological processi

    1. activation of MAPK activity Source: HGNC
    2. angiogenesis Source: MGI
    3. epidermal growth factor receptor signaling pathway Source: MGI
    4. mammary gland alveolus development Source: MGI
    5. negative regulation of apoptotic process Source: Ensembl
    6. positive regulation of cell division Source: UniProtKB-KW
    7. positive regulation of epidermal growth factor-activated receptor activity Source: HGNC
    8. positive regulation of epithelial cell proliferation Source: HGNC
    9. positive regulation of mitosis Source: HGNC
    10. response to drug Source: Ensembl
    11. wound healing Source: Ensembl

    Keywords - Molecular functioni

    Growth factor, Mitogen

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protransforming growth factor alpha
    Cleaved into the following chain:
    Alternative name(s):
    EGF-like TGF
    Short name:
    ETGF
    TGF type 1
    Gene namesi
    Name:Tgfa
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:98724. Tgfa.

    Subcellular locationi

    GO - Cellular componenti

    1. basolateral plasma membrane Source: Ensembl
    2. cell surface Source: Ensembl
    3. cytoplasmic vesicle Source: Ensembl
    4. extracellular space Source: UniProtKB-SubCell
    5. integral component of membrane Source: UniProtKB-KW
    6. nucleus Source: Ensembl
    7. perinuclear region of cytoplasm Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 159136Protransforming growth factor alphaPRO_0000302746Add
    BLAST
    Propeptidei24 – 3815Removed in mature formPRO_0000007758Add
    BLAST
    Chaini39 – 8850Transforming growth factor alphaPRO_0000007759Add
    BLAST
    Propeptidei89 – 15971Removed in mature formPRO_0000007760Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi25 – 251N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi46 ↔ 59PROSITE-ProRule annotation
    Disulfide bondi54 ↔ 70PROSITE-ProRule annotation
    Disulfide bondi72 ↔ 81PROSITE-ProRule annotation
    Lipidationi152 – 1521S-palmitoyl cysteineBy similarity
    Lipidationi153 – 1531S-palmitoyl cysteineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiP48030.
    PRIDEiP48030.

    Miscellaneous databases

    PMAP-CutDBP48030.

    Expressioni

    Gene expression databases

    ArrayExpressiP48030.
    BgeeiP48030.
    CleanExiMM_TGFA.
    GenevestigatoriP48030.

    Interactioni

    Subunit structurei

    Interacts with the PDZ domains of SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2 By similarity. Interacts with MAGI3.By similarity1 Publication

    Protein-protein interaction databases

    IntActiP48030. 1 interaction.
    MINTiMINT-1894871.
    STRINGi10090.ENSMUSP00000032066.

    Structurei

    3D structure databases

    ProteinModelPortaliP48030.
    SMRiP48030. Positions 39-88.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 9774ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini124 – 15936CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei98 – 12326HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini44 – 8340EGF-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 EGF-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG41326.
    HOGENOMiHOG000013036.
    HOVERGENiHBG000330.
    InParanoidiP48030.
    KOiK08774.
    OMAiCHSETGC.
    OrthoDBiEOG7VQJGP.
    PhylomeDBiP48030.
    TreeFamiTF332938.

    Family and domain databases

    InterProiIPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR015497. EGF_rcpt_ligand.
    [Graphical view]
    PANTHERiPTHR10740. PTHR10740. 1 hit.
    SMARTiSM00181. EGF. 1 hit.
    [Graphical view]
    PROSITEiPS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P48030-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVPATGQLAL LALGILLAVC QALENSTSPL SDSPVAAAVV SHFNKCPDSH    50
    TQYCFHGTCR FLVQEEKPAC VCHSGYVGVR CEHADLLAVV AASQKKQAIT 100
    ALVVVSIVAL AVLIITCVLI HCCQLRKHCE WCRALVCRHE KPSALLKGRT 150
    ACCHSETVV 159
    Length:159
    Mass (Da):17,018
    Last modified:February 1, 1996 - v1
    Checksum:iBE0AE8D89CE7DDFD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92420 mRNA. Translation: AAA40481.1.
    U65016 mRNA. Translation: AAB50554.1.
    U64873 Genomic DNA. Translation: AAB50553.1.
    CCDSiCCDS51832.1.
    PIRiS27195.
    RefSeqiNP_112476.1. NM_031199.3.
    UniGeneiMm.137222.

    Genome annotation databases

    EnsembliENSMUST00000032066; ENSMUSP00000032066; ENSMUSG00000029999.
    GeneIDi21802.
    KEGGimmu:21802.
    UCSCiuc009crk.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92420 mRNA. Translation: AAA40481.1 .
    U65016 mRNA. Translation: AAB50554.1 .
    U64873 Genomic DNA. Translation: AAB50553.1 .
    CCDSi CCDS51832.1.
    PIRi S27195.
    RefSeqi NP_112476.1. NM_031199.3.
    UniGenei Mm.137222.

    3D structure databases

    ProteinModelPortali P48030.
    SMRi P48030. Positions 39-88.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P48030. 1 interaction.
    MINTi MINT-1894871.
    STRINGi 10090.ENSMUSP00000032066.

    Proteomic databases

    PaxDbi P48030.
    PRIDEi P48030.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032066 ; ENSMUSP00000032066 ; ENSMUSG00000029999 .
    GeneIDi 21802.
    KEGGi mmu:21802.
    UCSCi uc009crk.2. mouse.

    Organism-specific databases

    CTDi 7039.
    MGIi MGI:98724. Tgfa.

    Phylogenomic databases

    eggNOGi NOG41326.
    HOGENOMi HOG000013036.
    HOVERGENi HBG000330.
    InParanoidi P48030.
    KOi K08774.
    OMAi CHSETGC.
    OrthoDBi EOG7VQJGP.
    PhylomeDBi P48030.
    TreeFami TF332938.

    Miscellaneous databases

    ChiTaRSi TGFA. mouse.
    NextBioi 301172.
    PMAP-CutDB P48030.
    PROi P48030.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P48030.
    Bgeei P48030.
    CleanExi MM_TGFA.
    Genevestigatori P48030.

    Family and domain databases

    InterProi IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR015497. EGF_rcpt_ligand.
    [Graphical view ]
    PANTHERi PTHR10740. PTHR10740. 1 hit.
    SMARTi SM00181. EGF. 1 hit.
    [Graphical view ]
    PROSITEi PS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and tissue distribution of mouse transforming growth factor-alpha."
      Vaughan T.J., Pascall J.C., Brown K.D.
      Biochim. Biophys. Acta 1132:322-324(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Characterization of the mouse transforming growth factor alpha gene: its expression during eyelid development and in waved 1 tissues."
      Berkowitz E.A., Seroogy K.B., Schroeder J.A., Russell W.E., Evans E.P., Riedel R.F., Phillips H.K., Harrison C.A., Lee D.C., Luetteke N.C.
      Cell Growth Differ. 7:1271-1282(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: 129/Sv.
      Tissue: Brain.
    3. "Identification of MAGI-3 as a transforming growth factor-alpha tail binding protein."
      Franklin J.L., Yoshiura K., Dempsey P.J., Bogatcheva G., Jeyakumar L., Meise K.S., Pearsall R.S., Threadgill D., Coffey R.J.
      Exp. Cell Res. 303:457-470(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MAGI3.

    Entry informationi

    Entry nameiTGFA_MOUSE
    AccessioniPrimary (citable) accession number: P48030
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3