Reviewed, UniProtKB/Swiss-Prot P48028 (CYSM_PSESY)

Names and origin

Protein names

Recommended name:
    Cysteine synthase B
      Short name=CSase B
    EC=2.5.1.47
Alternative name(s):
    O-acetylserine sulfhydrylase B
    O-acetylserine (thiol)-lyase B
      Short name=OAS-TL B

Gene names

Name:

cysM

Organism

Pseudomonas syringae pv. syringae

Taxonomic identifier

321 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae

Protein attributes

Sequence length	169 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›169	›169	Cysteine synthase B		PRO_0000167114
Sites
	Binding site	75	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	45	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Non-terminal residue	169	1

Sequences

	Sequence		Length	Mass (Da)
	P48028-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: E12781E90CA77F87	FASTA	169	18,023
	10 20 30 40 50 60 MTLQYQTIAD CVGNTPLVRL QRMAGNTSNT LLLKLEGNNP AGSVKDRPAL SMITRAELRG 70 80 90 100 110 120 QIHPGDTLIE ATSGNTGIAL AMAAAIKGYR MILIMPDNSS AERKAAMTAY GAELISVSKD 130 140 150 160 DGMEGARDLA ERMQAEGRGK VLDQFANGDN PEAHYTSTGP EIWRQTGGT

References

[1]	"The lemA gene required for pathogenicity of Pseudomonas syringae pv. syringae on bean is a member of a family of two-component regulators." Hrabak E.M., Willis D.K. J. Bacteriol. 174:3011-3020(1992) [PubMed: 1314807] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M80477 Genomic DNA. Translation: AAA25876.1.
PIR	A41863.
3D structure databases
HSSP	HSSP built from PDB template 1J6N based on UniProtKB Q9WZD3.
SMR	P48028. Positions 7-168.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.47. 294409.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSM_PSESY

Accession

Primary (citable) accession number: P48028

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	September 1, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)