Reviewed, UniProtKB/Swiss-Prot P48022 (HMDH2_SOLLC)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase 2
      Short name=HMG-CoA reductase 2
    EC=1.1.1.34

Gene names

Name:

HMG2

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP(+) = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 602	602	3-hydroxy-3-methylglutaryl-coenzyme A reductase 2		PRO_0000114443
Regions
	Transmembrane	44 – 67	24	Potential
	Transmembrane	95 – 115	21	Potential
	Region	116 – 187	72	Linker By similarity
	Region	188 – 602	415	Catalytic By similarity
	Compositional bias	30 – 34	5	Poly-Gln
Sites
	Active site	281	1	Charge relay system By similarity
	Active site	413	1	Charge relay system By similarity
	Active site	489	1	Charge relay system By similarity
	Active site	587	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	124	1	N-linked (GlcNAc...) Potential
	Glycosylation	345	1	N-linked (GlcNAc...) Potential
	Glycosylation	458	1	N-linked (GlcNAc...) Potential
	Glycosylation	591	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	56	1	Missing in AAB62581. Ref.2
	Sequence conflict	186	1	G → E in AAB62581. Ref.2
	Sequence conflict	339	1	S → T in AAB62581. Ref.2
	Sequence conflict	369 – 370	2	LC → FV in AAB62581. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P48022-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 0266E55E2EB953BD	FASTA	602	64,715
	10 20 30 40 50 60 MDVRRRSEEP VYPSKVFAAD EKPLKPHKKQ QQQQEDKNTL LIDASDALPL PLYLTTNGLF 70 80 90 100 110 120 FTMFFSVMYF LLSRWREKIR NSTPLHVVTL SELGAIVSLI ASVIYLLGFF GIGFVQTFVS 130 140 150 160 170 180 RGNNDSWDEN DEEFLLKEDS RCGPATTLGC AVPAPPARQI APMAPPQPSM SMVEKPAPLI 190 200 210 220 230 240 TSASSGEDEE IIKSVVQGKI PSYSLESKLG DCKRAASIRK EVMQRITGKS LEGLPLEGFN 250 260 270 280 290 300 YESILGQCCE MPIGYVQIPV GIAGPLLLNG KEFSVPMATT EGCLVASTNR GCKAIYASGG 310 320 330 340 350 360 ATCILLRDGM TRAPCVRFGT AKRAAELKFF VEDPIKFESL ANVFNQSSRF ARLQRIQCAI 370 380 390 400 410 420 AGKNLYMRLC CSTGDAMGMN MVSKGVQNVL DYLQNEYPDM DVIGISGNFC SDKKPAAVNW 430 440 450 460 470 480 IEGRGKSVVC EAIITEEVVK KVLKTEVAAL VELNMLKNLT GSAMAGALGG FNAHASNIVS 490 500 510 520 530 540 AVFIATGQDP AQNIESSHCI TMMEAVNDGK DLHISVTMPS IEVGTVGGGT QLASQSACLN 550 560 570 580 590 600 LLGVKGANRE APGSNARLLA TVVAGSVLAG ELSLMSAISS GQLVNSHMKY NRSTKDVTKA SS

References

[1]	"Structure and nucleotide sequence of tomato HMG2 encoding 3-hydroxy-3-methyl-glutaryl coenzyme A reductase." Park H.S., Denbow C.J., Cramer C.L. Plant Mol. Biol. 20:327-331(1992) [PubMed: 1391777] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. VFNT Cherry.
[2]	"The promoter for tomato 3-hydroxy-3-methylglutaryl coenzyme A reductase gene 2 has unusual regulatory elements that direct high-level expression." Daraselia N.D., Tarchevskaya S., Narita J.O. Plant Physiol. 112:727-733(1996) [PubMed: 8883384] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M63642 Genomic DNA. Translation: AAA34169.1. U68072 Genomic DNA. Translation: AAB62581.1.
PIR	S25316.
3D structure databases
HSSP	HSSP built from PDB template 1DQA based on UniProtKB P04035.
ModBase	Search...
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH2_SOLLC

Accession

Primary (citable) accession number: P48022
Secondary accession number(s): O24028

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 25, 2008
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)