P48021 (HMDH_CAMAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: 3-hydroxy-3-methylglutaryl-coenzyme A reductase Short name=HMG-CoA reductase EC=1.1.1.34
Organism	Camptotheca acuminata (Happy tree)
Taxonomic identifier	16922 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › Cornales › Cornaceae › Camptotheca

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 593	593	3-hydroxy-3-methylglutaryl-coenzyme A reductase		PRO_0000114435
Regions
	Transmembrane	52 – 72	21	Helical; Potential
	Transmembrane	94 – 114	21	Helical; Potential
	Region	115 – 177	63	Linker By similarity
	Region	178 – 593	416	Catalytic By similarity
Sites
	Active site	272	1	Charge relay system By similarity
	Active site	404	1	Charge relay system By similarity
	Active site	480	1	Charge relay system By similarity
	Active site	578	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	336	1	N-linked (GlcNAc...) Potential
	Glycosylation	449	1	N-linked (GlcNAc...) Potential
	Glycosylation	582	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P48021 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: F22CB55B2E4944A7	FASTA	593	63,278
	10 20 30 40 50 60 MDVRRRSINS IHQIPSVGGT APPMLKPKQP TKVDAVDLPD SPKASDALPL PLYITNGVFF 70 80 90 100 110 120 TLFFTVVYYL LVRWREKIRN STPLHVVTLS EIAAIFTFVA SFIYLLGFFG IGLVQPFTSR 130 140 150 160 170 180 SSHDDVWGVD DDEDVDEIVL KEDTRTVPCA AAPVDCPLPP IKPKVVDPVP ISPPSSEEDE 190 200 210 220 230 240 EIIKSVVEGT TPSYALESKL GDSHRAAAIR REALQRMTKK SLAGLPLDGF DYDSILGQCC 250 260 270 280 290 300 EMPVGYVQIP VGIAGPLLLD GREYSVPMAT TEGCLVASTN RGCKAIFACG GATSVLLRDA 310 320 330 340 350 360 MTRAPVVRFG SAKRAADLKF FLENPLNFET LAAVFNSSSR FGKLQNIKCA IAGKNLYMRY 370 380 390 400 410 420 SCSTGDAMGM NMISKGVQNV LDFLQDDFPD MDVIGISGNY CSDKKPAAVN WIEGRGKSVV 430 440 450 460 470 480 CEAVIKEEVV KKVLKTNVAS LVELNMLKNL TGSAMAGALG GFNAHASNIV SAVYLATGQD 490 500 510 520 530 540 PAQNVESSHC ITMMEAINDG KDLHVSVTMP SIEVGTVGGG TQLASQSACL NLLGVKGASK 550 560 570 580 590 EAPGSNARLL ATIVAGSVLA GELSLMSAIA AGQLVNSHMK YNRSNKDVTK ASS

References

[1]	"Expression of a 3-hydroxy-3-methylglutaryl coenzyme A reductase gene from Camptotheca acuminata is differentially regulated by wounding and methyl jasmonate." Burnett R.J., Maldonado-Mendoza I.E., McKnight T.D., Nessler C.L. Plant Physiol. 103:41-48(1993) [PubMed: 8208857] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L10390 Genomic DNA. Translation: AAA33040.1.
3D structure databases
ProteinModelPortal	P48021.
SMR	P48021. Positions 175-575.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase. IPR023074. HMG_CoA_Rdtase_cat. IPR023076. HMG_CoA_Rdtase_CS. IPR004554. HMG_CoA_Rdtase_eu_arc. IPR023282. HMG_CoA_Rdtase_N. IPR009023. HMG_CoA_Rdtase_NAD(P)-bd. IPR009029. HMG_CoA_Rdtase_sub-bd. [Graphical view]
Gene3D	G3DSA:3.30.70.420. G3DSA:3.30.70.420. 1 hit. G3DSA:3.90.770.10. HMG-CoA_red. 2 hits. G3DSA:1.10.3270.10. HMG_CoA_Rdtase_N. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
SUPFAM	SSF55035. HMG_CoA_NAD_bind. 1 hit. SSF56542. HMG_CoA_sub_bind. 1 hit.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH_CAMAC

Accession

Primary (citable) accession number: P48021

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 28, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program