P48020 (HMDH1_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
Short name=HMG-CoA reductase 1
Short name=HMGR
Short name=HMGR1
EC=1.1.1.34

Gene names

Name:

HMG1

Organism

Solanum tuberosum (Potato)

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in flower primordia and anthers.
Induction	Induced by wounding. This enhancement is greatly reduced by treatment with arachidonic acid or after innoculation with the fungal pathogen, P.infestans.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 596	596	3-hydroxy-3-methylglutaryl-coenzyme A reductase 1		PRO_0000114448
Regions
	Transmembrane	41 – 61	21	Helical; Potential
	Transmembrane	83 – 103	21	Helical; Potential
	Region	104 – 183	80	Linker By similarity
	Region	184 – 596	413	Catalytic By similarity
Sites
	Active site	278	1	Charge relay system By similarity
	Active site	410	1	Charge relay system By similarity
	Active site	486	1	Charge relay system By similarity
	Active site	584	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	342	1	N-linked (GlcNAc...) Potential
	Glycosylation	455	1	N-linked (GlcNAc...) Potential
	Glycosylation	588	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P48020 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 4E7060D3ADC6EE60	FASTA	596	63,917
	10 20 30 40 50 60 MDVRRRPVKP LYTSKDASAG EPLKQQEVSS PKASDALPLP LYLTNGLFFT MFFSVMYFLL 70 80 90 100 110 120 VRWREKIRNS IPLHVVTLSE LLAMVSLIAS VIYLLGFFGI GFVQSFVSRS NSDSWDIEDE 130 140 150 160 170 180 NAEQLIIEED SRRGPCAAAT TLGCVVPPPP VRKIAPMVPQ QPAKVALSQT EKPSPIIMPA 190 200 210 220 230 240 LSEDDEEIIQ SVVQGKTPSY SLESKLGDCM RAASIRKEAL QRITGKSLEG LPLEGFDYSS 250 260 270 280 290 300 ILGQCCEMPV GYVQIPVGIA GPLLLDGREY SVPMATTEGC LVASTNRGCK AIFVSGGADS 310 320 330 340 350 360 VLLRDGMTRA PVVRFTTAKR AAELKFFVED PLNFETLSLM FNKSSRFARL QGIQCAIAGK 370 380 390 400 410 420 NLYITFSCST GDAMGMNMVS KGVQNVLDYL QSEYPDMDVI GISGNFCSDK KPAAVNWIEG 430 440 450 460 470 480 RGKSVVCEAI IKEEVVKKVL KTEVAALVEL NMLKNLTGSA MAGALGGFNA HASNIVSAVY 490 500 510 520 530 540 LATGQDPAQN VESSHCITMM EAVNDGKDLH VSVTMPSIEV GTVGGGTQLA SQSACLNLLG 550 560 570 580 590 VKGANRDAPG SNARLLATIV AGSVLAGELS LMSAISAGQL VKSHMKYNRS IKDISK

References

[1]	"Differential induction and suppression of potato 3-hydroxy-3-methylglutaryl coenzyme A reductase genes in response to Phytophthora infestans and to its elicitor arachidonic acid." Choi D., Ward B.L., Bostock R.M. Plant Cell 4:1333-1344(1992) [PubMed: 1283354] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Kennebec. Tissue: Tuber.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L01400 mRNA. Translation: AAA93498.1.
PIR	S59944.
3D structure databases
ProteinModelPortal	P48020.
SMR	P48020. Positions 183-581.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase. IPR023074. HMG_CoA_Rdtase_cat. IPR023076. HMG_CoA_Rdtase_CS. IPR004554. HMG_CoA_Rdtase_eu_arc. IPR023282. HMG_CoA_Rdtase_N. IPR009023. HMG_CoA_Rdtase_NAD(P)-bd. IPR009029. HMG_CoA_Rdtase_sub-bd. [Graphical view]
Gene3D	G3DSA:3.30.70.420. G3DSA:3.30.70.420. 1 hit. G3DSA:3.90.770.10. HMG-CoA_red. 2 hits. G3DSA:1.10.3270.10. HMG_CoA_Rdtase_N. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
SUPFAM	SSF55035. HMG_CoA_NAD_bind. 1 hit. SSF56542. HMG_CoA_sub_bind. 1 hit.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. False negative. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH1_SOLTU

Accession

Primary (citable) accession number: P48020

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 28, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program