Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vacuolar acid trehalase

Gene

ATH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Alpha,alpha-trehalose + H2O = beta-D-glucose + alpha-D-glucose.

GO - Molecular functioni

  • alpha,alpha-trehalase activity Source: SGD
  • carbohydrate binding Source: InterPro

GO - Biological processi

  • cellular response to desiccation Source: SGD
  • cellular response to ethanol Source: SGD
  • cellular response to freezing Source: SGD
  • trehalose catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17145.
YEAST:YPR026W-MONOMER.

Protein family/group databases

CAZyiGH65. Glycoside Hydrolase Family 65.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar acid trehalase (EC:3.2.1.28)
Alternative name(s):
Alpha,alpha-trehalase
Alpha,alpha-trehalose glucohydrolase
Gene namesi
Name:ATH1
Ordered Locus Names:YPR026W
ORF Names:YP9367.06
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR026W.
SGDiS000006230. ATH1.

Subcellular locationi

  • Vacuole

  • Note: Lysosome-like vacuoles.Curated

GO - Cellular componenti

  • cell wall-bounded periplasmic space Source: SGD
  • fungal-type cell wall Source: SGD
  • fungal-type vacuole Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2366477.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 1211Vacuolar acid trehalasePRO_0000012110
Signal peptidei1 – ?Sequence analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi28 – 281N-linked (GlcNAc...)Sequence analysis
Glycosylationi32 – 321N-linked (GlcNAc...)Sequence analysis
Glycosylationi98 – 981N-linked (GlcNAc...)Sequence analysis
Glycosylationi207 – 2071N-linked (GlcNAc...)Sequence analysis
Glycosylationi238 – 2381N-linked (GlcNAc...)Sequence analysis
Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence analysis
Glycosylationi255 – 2551N-linked (GlcNAc...)Sequence analysis
Glycosylationi259 – 2591N-linked (GlcNAc...)Sequence analysis
Glycosylationi325 – 3251N-linked (GlcNAc...)Sequence analysis
Glycosylationi370 – 3701N-linked (GlcNAc...)Sequence analysis
Glycosylationi376 – 3761N-linked (GlcNAc...)Sequence analysis
Glycosylationi488 – 4881N-linked (GlcNAc...)Sequence analysis
Glycosylationi539 – 5391N-linked (GlcNAc...)Sequence analysis
Glycosylationi568 – 5681N-linked (GlcNAc...)Sequence analysis
Glycosylationi628 – 6281N-linked (GlcNAc...)Sequence analysis
Glycosylationi638 – 6381N-linked (GlcNAc...)Sequence analysis
Glycosylationi696 – 6961N-linked (GlcNAc...)Sequence analysis
Glycosylationi705 – 7051N-linked (GlcNAc...)Sequence analysis
Glycosylationi879 – 8791N-linked (GlcNAc...)Sequence analysis
Glycosylationi897 – 8971N-linked (GlcNAc...)Sequence analysis
Glycosylationi910 – 9101N-linked (GlcNAc...)Sequence analysis
Glycosylationi972 – 9721N-linked (GlcNAc...)Sequence analysis
Glycosylationi990 – 9901N-linked (GlcNAc...)Sequence analysis
Glycosylationi1031 – 10311N-linked (GlcNAc...)Sequence analysis
Glycosylationi1049 – 10491N-linked (GlcNAc...)Sequence analysis
Glycosylationi1064 – 10641N-linked (GlcNAc...)Sequence analysis
Glycosylationi1147 – 11471N-linked (GlcNAc...)Sequence analysis
Glycosylationi1157 – 11571N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP48016.

PTM databases

iPTMnetiP48016.

Interactioni

Protein-protein interaction databases

BioGridi36203. 20 interactions.
IntActiP48016. 1 interaction.
MINTiMINT-4988238.

Structurei

3D structure databases

ProteinModelPortaliP48016.
SMRiP48016. Positions 314-828.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 65 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00390000006297.
HOGENOMiHOG000246546.
InParanoidiP48016.
KOiK01194.
OMAiEFAYNIN.
OrthoDBiEOG76MKHN.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
2.70.98.40. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR011013. Gal_mutarotase_SF_dom.
IPR005195. Glyco_hydro_65_M.
IPR005196. Glyco_hydro_65_N.
[Graphical view]
PfamiPF03632. Glyco_hydro_65m. 1 hit.
PF03636. Glyco_hydro_65N. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF74650. SSF74650. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48016-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRIRSLWFN AEASYSNLNN SPSLRNKNST GNNSRSKNYR SFSRFDLINS
60 70 80 90 100
ILLLMMLFLL AIFVTALYLT KSSRLTYSHA SRAALFNPLG VISPSLGNHT
110 120 130 140 150
LNYDPEARES SKKLYELLSD FNTAYYDDEN MILGSNLFSK NTYSRQPYVA
160 170 180 190 200
NGYIGSRIPN IGFGYALDTL NFYTDAPGAL NNGWPLRNHR FAGAFVSDFY
210 220 230 240 250
CLQPKLNSTN FPELDDVGYS TVISSIPQWT NLQFSLVNDS KWFNPQNVTL
260 270 280 290 300
DDVTNYSQNL SMKDGIVTTE LDWLNSQIHV KSEIWAHRHI HPLGVVSLEI
310 320 330 340 350
SLNTDHLPSD FDSLDVNIWD ILDFNTSHRT VLHSTGTDEK NNAVFMIVQP
360 370 380 390 400
DNVPSSNCAI YSTCTVKYEN STNPINSSES FEEKDVSSNI YNVILTEDQP
410 420 430 440 450
KIIVHKYVGI MSTEFNKNKE QQDNTNIGLA KMIALNSKGN YEKLLSSHKR
460 470 480 490 500
AWYDLYNDAF IEIPSDSLLE MTARSSLFHL LANTRDYNVS SDRGLPVGVS
510 520 530 540 550
GLSSDSYGGM VFWDADIWME PALLPFFPNV AQNMNNYRNA THSQAKLNAE
560 570 580 590 600
KYGYPGAIYP WTSGKYANCT STGPCVDYEY HINVDVAMAS FSIYLNGHEG
610 620 630 640 650
IDDEYLRYTT WPIIKNAAQF FTAYVKYNSS LGLYETYNLT DPDEFANHIN
660 670 680 690 700
NGAFTNAGIK TLLKWATDIG NHLGEVVDPK WSEISKDIYI PRSSSNITLE
710 720 730 740 750
YSGMNSSVEI KQADVTLMVY PLGYINDESI LNNAIKDLYY YSERQSASGP
760 770 780 790 800
AMTYPVFVAA AAGLLNHGSS SQSYLYKSVL PYLRAPFAQF SEQSDDNFLT
810 820 830 840 850
NGLTQPAFPF LTANGGFLQS ILFGLTGIRY SYEVDPDTKK INRLLRFNPI
860 870 880 890 900
ELPLLPGGIA IRNFKYMNQV LDIIIDDHNG TIVHKSGDVP IHIKIPNRSL
910 920 930 940 950
IHDQDINFYN GSENERKPNL ERRDVDRVGD PMRMDRYGTY YLLKPKQELT
960 970 980 990 1000
VQLFKPGLNA RNNIAENKQI TNLTAGVPGD VAFSALDGNN YTHWQPLDKI
1010 1020 1030 1040 1050
HRAKLLIDLG EYNEKEITKG MILWGQRPAK NISISILPHS EKVENLFANV
1060 1070 1080 1090 1100
TEIMQNSGND QLLNETIGQL LDNAGIPVEN VIDFDGIEQE DDESLDDVQA
1110 1120 1130 1140 1150
LLHWKKEDLA KLIEQIPRLN FLKRKFVKIL DNVPVSPSEP YYEASRNQSL
1160 1170 1180 1190 1200
IEILPSNRTT FTIDYDKLQV GDKGNTDWRK TRYIVVAVQG VYDDYDDDNK
1210
GATIKEIVLN D
Length:1,211
Mass (Da):136,921
Last modified:February 1, 1996 - v1
Checksum:i34AEC44B0B648DEC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1168 – 121144LQVGD…IVLND → FAGG in CAA58961 (PubMed:7502577).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84156 Genomic DNA. Translation: CAA58961.1.
Z49274 Genomic DNA. Translation: CAA89280.1.
Z71255 Genomic DNA. Translation: CAA95022.1.
BK006949 Genomic DNA. Translation: DAA11452.1.
PIRiS54500.
RefSeqiNP_015351.1. NM_001184123.1.

Genome annotation databases

EnsemblFungiiYPR026W; YPR026W; YPR026W.
GeneIDi856137.
KEGGisce:YPR026W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84156 Genomic DNA. Translation: CAA58961.1.
Z49274 Genomic DNA. Translation: CAA89280.1.
Z71255 Genomic DNA. Translation: CAA95022.1.
BK006949 Genomic DNA. Translation: DAA11452.1.
PIRiS54500.
RefSeqiNP_015351.1. NM_001184123.1.

3D structure databases

ProteinModelPortaliP48016.
SMRiP48016. Positions 314-828.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36203. 20 interactions.
IntActiP48016. 1 interaction.
MINTiMINT-4988238.

Chemistry

ChEMBLiCHEMBL2366477.

Protein family/group databases

CAZyiGH65. Glycoside Hydrolase Family 65.

PTM databases

iPTMnetiP48016.

Proteomic databases

MaxQBiP48016.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR026W; YPR026W; YPR026W.
GeneIDi856137.
KEGGisce:YPR026W.

Organism-specific databases

EuPathDBiFungiDB:YPR026W.
SGDiS000006230. ATH1.

Phylogenomic databases

GeneTreeiENSGT00390000006297.
HOGENOMiHOG000246546.
InParanoidiP48016.
KOiK01194.
OMAiEFAYNIN.
OrthoDBiEOG76MKHN.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17145.
YEAST:YPR026W-MONOMER.

Miscellaneous databases

NextBioi981242.
PROiP48016.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
2.70.98.40. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR011013. Gal_mutarotase_SF_dom.
IPR005195. Glyco_hydro_65_M.
IPR005196. Glyco_hydro_65_N.
[Graphical view]
PfamiPF03632. Glyco_hydro_65m. 1 hit.
PF03636. Glyco_hydro_65N. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF74650. SSF74650. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of a novel yeast gene, ATH1, that is required for vacuolar acid trehalase activity."
    Destruelle M., Holzer H., Klionsky D.J.
    Yeast 11:1015-1025(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 130-1211.
    Strain: MW303-1B.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Purification and biochemical characterization of the ATH1 gene product, vacuolar acid trehalase, from Saccharomyces cerevisiae."
    Alizadeh P., Klionsky D.J.
    FEBS Lett. 391:273-278(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 368-381 AND 385-396, GLYCOSYLATION.

Entry informationi

Entry nameiATH1_YEAST
AccessioniPrimary (citable) accession number: P48016
Secondary accession number(s): D6W436
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 11, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.