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Protein

Aminomethyltransferase, mitochondrial

Gene

GCV1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The glycine cleavage system (glycine decarboxylase complex) catalyzes the degradation of glycine.

Catalytic activityi

[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei221 – 2211SubstrateBy similarity
Binding sitei250 – 2501SubstrateBy similarity
Binding sitei397 – 3971SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

  • glycine catabolic process Source: SGD
  • glycine metabolic process Source: SGD
  • one-carbon metabolic process Source: SGD
  • oxidation-reduction process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YDR019C-MONOMER.
ReactomeiR-SCE-6783984. Glycine degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminomethyltransferase, mitochondrial (EC:2.1.2.10)
Alternative name(s):
Glycine cleavage system T protein
Short name:
GCVT
Glycine decarboxylase complex subunit T
Gene namesi
Name:GCV1
Ordered Locus Names:YDR019C
ORF Names:YD9335.05C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR019C.
SGDiS000002426. GCV1.

Subcellular locationi

GO - Cellular componenti

  • glycine cleavage complex Source: SGD
  • mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 400Aminomethyltransferase, mitochondrialPRO_0000010766
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiP48015.

Interactioni

Subunit structurei

Component of the glycine decarboxylase complex (GDC), which is composed of four proteins: P, T, L and H.

Protein-protein interaction databases

BioGridi32069. 20 interactions.
IntActiP48015. 3 interactions.
MINTiMINT-4480278.

Structurei

3D structure databases

ProteinModelPortaliP48015.
SMRiP48015. Positions 16-365.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvT family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00530000063120.
HOGENOMiHOG000239380.
InParanoidiP48015.
KOiK00605.
OMAiYGNDIST.
OrthoDBiEOG092C3LQP.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
InterProiIPR006223. GCS_T.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PANTHERiPTHR13847:SF5. PTHR13847:SF5. 1 hit.
PfamiPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006487. GcvT. 1 hit.
SUPFAMiSSF101790. SSF101790. 1 hit.
TIGRFAMsiTIGR00528. gcvT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48015-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIIKKIVFK RFNSTLKKTA LHDLHVSLGG TMVPYAGYSM PVLYKGQTHI
60 70 80 90 100
ESHNWTRTNA GLFDVSHMLQ SKLSGPHSVK FLQRVTPTDF NALPVGSGTL
110 120 130 140 150
SVLLNPQGGV VDDTIITKEN DDNEFYIVTN AGCAERDTEF FHDELQNGST
160 170 180 190 200
LDCQWKIIEG RSLLALQGPK AKDVLEPLLS KTAPGKDLKE LFFGQRHEFA
210 220 230 240 250
LKDGSLVQIA RGGYTGEDGF EISIANEKAV EFAEQLLANP VMKPIGLAAR
260 270 280 290 300
DSLRLEAGMC LYGHELDESI TPVEAALNWV ISKSRRDLVD QKYWFNGYAK
310 320 330 340 350
IMDQLNNKTY SKVRVGFKYL KKGPAARNGV KIFLPDAETE VGLVTSGSAS
360 370 380 390 400
PTLNNINIGQ AYVQKGYHKK GTKLLVQVRN KFYPIELAKM PLVPTHYYKQ
Length:400
Mass (Da):44,469
Last modified:October 1, 1996 - v2
Checksum:iFFA349B0E0176D65
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti122 – 1221D → E in AAB05000 (PubMed:9047339).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41522 Genomic DNA. Translation: AAB05000.1.
X95966 Genomic DNA. Translation: CAA65211.1.
Z49770 Genomic DNA. Translation: CAA89844.1.
Z74315 Genomic DNA. Translation: CAA98840.1.
BK006938 Genomic DNA. Translation: DAA11865.1.
PIRiS54642.
RefSeqiNP_010302.1. NM_001180327.1.

Genome annotation databases

EnsemblFungiiYDR019C; YDR019C; YDR019C.
GeneIDi851582.
KEGGisce:YDR019C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41522 Genomic DNA. Translation: AAB05000.1.
X95966 Genomic DNA. Translation: CAA65211.1.
Z49770 Genomic DNA. Translation: CAA89844.1.
Z74315 Genomic DNA. Translation: CAA98840.1.
BK006938 Genomic DNA. Translation: DAA11865.1.
PIRiS54642.
RefSeqiNP_010302.1. NM_001180327.1.

3D structure databases

ProteinModelPortaliP48015.
SMRiP48015. Positions 16-365.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32069. 20 interactions.
IntActiP48015. 3 interactions.
MINTiMINT-4480278.

Proteomic databases

MaxQBiP48015.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR019C; YDR019C; YDR019C.
GeneIDi851582.
KEGGisce:YDR019C.

Organism-specific databases

EuPathDBiFungiDB:YDR019C.
SGDiS000002426. GCV1.

Phylogenomic databases

GeneTreeiENSGT00530000063120.
HOGENOMiHOG000239380.
InParanoidiP48015.
KOiK00605.
OMAiYGNDIST.
OrthoDBiEOG092C3LQP.

Enzyme and pathway databases

BioCyciYEAST:YDR019C-MONOMER.
ReactomeiR-SCE-6783984. Glycine degradation.

Miscellaneous databases

PROiP48015.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
InterProiIPR006223. GCS_T.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PANTHERiPTHR13847:SF5. PTHR13847:SF5. 1 hit.
PfamiPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006487. GcvT. 1 hit.
SUPFAMiSSF101790. SSF101790. 1 hit.
TIGRFAMsiTIGR00528. gcvT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGCST_YEAST
AccessioniPrimary (citable) accession number: P48015
Secondary accession number(s): D6VS05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4090 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.