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Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC4

Gene

rpc10

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi24 – 241ZincBy similarity
Metal bindingi27 – 271ZincBy similarity
Metal bindingi41 – 411ZincBy similarity
Metal bindingi44 – 441ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri24 – 4421C4-typeSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • transcription from RNA polymerase III promoter Source: PomBase
  • transcription from RNA polymerase II promoter Source: PomBase
  • transcription from RNA polymerase I promoter Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SPO-113418. Formation of the Early Elongation Complex.
R-SPO-1834949. Cytosolic sensors of pathogen-associated DNA.
R-SPO-674695. RNA Polymerase II Pre-transcription Events.
R-SPO-6781823. Formation of TC-NER Pre-Incision Complex.
R-SPO-6782135. Dual incision in TC-NER.
R-SPO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SPO-72086. mRNA Capping.
R-SPO-72165. mRNA Splicing - Minor Pathway.
R-SPO-73762. RNA Polymerase I Transcription Initiation.
R-SPO-73776. RNA Polymerase II Promoter Escape.
R-SPO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SPO-75953. RNA Polymerase II Transcription Initiation.
R-SPO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SPO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I, II, and III subunit RPABC4
Short name:
RNA polymerases I, II, and III subunit ABC4
Alternative name(s):
ABC10-alpha
Gene namesi
Name:rpc10
Synonyms:rpb12
ORF Names:SPBC19C2.03
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC19C2.03.
PomBaseiSPBC19C2.03. rpc10.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • DNA-directed RNA polymerase I complex Source: PomBase
  • DNA-directed RNA polymerase II, core complex Source: PomBase
  • DNA-directed RNA polymerase III complex Source: PomBase
  • nucleolus Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6363DNA-directed RNA polymerases I, II, and III subunit RPABC4PRO_0000159752Add
BLAST

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of 14, 12 and 17 subunits, respectively.By similarity

Protein-protein interaction databases

BioGridi277312. 9 interactions.
IntActiP48011. 1 interaction.
MINTiMINT-1214266.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H0GX-ray3.65L/X1-63[»]
ProteinModelPortaliP48011.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP48011.

Family & Domainsi

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri24 – 4421C4-typeSequence analysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000191755.
InParanoidiP48011.
KOiK03009.
OMAiCGAENQL.
OrthoDBiEOG7K0ZRP.
PhylomeDBiP48011.

Family and domain databases

Gene3Di2.20.28.30. 1 hit.
InterProiIPR006591. RNAP_P/RPABC4.
IPR029040. RPABC4/Spt4.
[Graphical view]
PfamiPF03604. DNA_RNApol_7kD. 1 hit.
[Graphical view]
SMARTiSM00659. RPOLCX. 1 hit.
[Graphical view]
SUPFAMiSSF63393. SSF63393. 1 hit.

Sequencei

Sequence statusi: Complete.

P48011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNHPTSTGGT AFNPPRPATM IYLCADCGAR NTIQAKEVIR CRECGHRVMY
60
KMRTKRMVQF EAR
Length:63
Mass (Da):7,203
Last modified:July 15, 1998 - v2
Checksum:i97EBE50FAA95E884
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291A → R (PubMed:7651387).Curated
Sequence conflicti29 – 291A → R (PubMed:9290054).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20867 mRNA. Translation: AAA80487.1.
U80217 mRNA. Translation: AAC49841.1.
AF027819 Genomic DNA. Translation: AAC16896.1.
D89634 Genomic DNA. Translation: BAA22807.1.
CU329671 Genomic DNA. Translation: CAB52030.1.
PIRiT43546.
T52536.
RefSeqiNP_595688.1. NM_001021585.2.

Genome annotation databases

EnsemblFungiiSPBC19C2.03.1; SPBC19C2.03.1:pep; SPBC19C2.03.
GeneIDi2540793.
KEGGispo:SPBC19C2.03.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20867 mRNA. Translation: AAA80487.1.
U80217 mRNA. Translation: AAC49841.1.
AF027819 Genomic DNA. Translation: AAC16896.1.
D89634 Genomic DNA. Translation: BAA22807.1.
CU329671 Genomic DNA. Translation: CAB52030.1.
PIRiT43546.
T52536.
RefSeqiNP_595688.1. NM_001021585.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H0GX-ray3.65L/X1-63[»]
ProteinModelPortaliP48011.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277312. 9 interactions.
IntActiP48011. 1 interaction.
MINTiMINT-1214266.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC19C2.03.1; SPBC19C2.03.1:pep; SPBC19C2.03.
GeneIDi2540793.
KEGGispo:SPBC19C2.03.

Organism-specific databases

EuPathDBiFungiDB:SPBC19C2.03.
PomBaseiSPBC19C2.03. rpc10.

Phylogenomic databases

HOGENOMiHOG000191755.
InParanoidiP48011.
KOiK03009.
OMAiCGAENQL.
OrthoDBiEOG7K0ZRP.
PhylomeDBiP48011.

Enzyme and pathway databases

ReactomeiR-SPO-113418. Formation of the Early Elongation Complex.
R-SPO-1834949. Cytosolic sensors of pathogen-associated DNA.
R-SPO-674695. RNA Polymerase II Pre-transcription Events.
R-SPO-6781823. Formation of TC-NER Pre-Incision Complex.
R-SPO-6782135. Dual incision in TC-NER.
R-SPO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SPO-72086. mRNA Capping.
R-SPO-72165. mRNA Splicing - Minor Pathway.
R-SPO-73762. RNA Polymerase I Transcription Initiation.
R-SPO-73776. RNA Polymerase II Promoter Escape.
R-SPO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SPO-75953. RNA Polymerase II Transcription Initiation.
R-SPO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SPO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

EvolutionaryTraceiP48011.
PROiP48011.

Family and domain databases

Gene3Di2.20.28.30. 1 hit.
InterProiIPR006591. RNAP_P/RPABC4.
IPR029040. RPABC4/Spt4.
[Graphical view]
PfamiPF03604. DNA_RNApol_7kD. 1 hit.
[Graphical view]
SMARTiSM00659. RPOLCX. 1 hit.
[Graphical view]
SUPFAMiSSF63393. SSF63393. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Four subunits that are shared by the three classes of RNA polymerase are functionally interchangeable between Homo sapiens and Saccharomyces cerevisiae."
    Shpakovski G.V., Acker J., Wintzerith M., Lacroix J.F., Thuriaux P., Vigneron M.
    Mol. Cell. Biol. 15:4702-4710(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 972 / ATCC 24843.
  2. "Molecular cloning and characterization of cDNA of the rpc10+ gene encoding the smallest subunit of nuclear RNA polymerases of Schizosaccharomyces pombe."
    Shpakovskii G.V., Lebedenko E.N.
    Bioorg. Khim. 23:441-448(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 972 / ATCC 24843.
  3. "Exon-intron organization rpb10+ and rpc10+ genes of Schizosaccharomyces pombe, coding for mini-subunits of nuclear RNA-polymerase I-III."
    Shpakovskii G.V., Proshkin S.A., Lebedenko E.N.
    Mol. Biol. (Mosk.) 32:285-290(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "Gene organization and protein sequence of the small subunits of Schizosaccharomyces pombe RNA polymerase II."
    Sakurai H., Ishihama A.
    Gene 196:165-174(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: JY741.
  5. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiRPAB4_SCHPO
AccessioniPrimary (citable) accession number: P48011
Secondary accession number(s): O14457
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 15, 1998
Last modified: June 8, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.