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Protein

Floral homeotic protein PISTILLATA

Gene

PI

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable transcription factor involved in the genetic control of flower development. Is required for normal development of petals and stamens in the wild-type flower. Forms a heterodimer with APETALA3 that is required for autoregulation of both AP3 and PI genes. AP3/PI heterodimer interacts with APETALA1 or SEPALLATA3 to form a ternary complex that could be responsible for the regulation of the genes involved in the flower development. AP3/PI heterodimer activates the expression of NAP.2 Publications

Miscellaneous

Mutations in PI cause transformation of petals into sepals and stamina into carpels.

GO - Molecular functioni

  • DNA binding Source: TAIR
  • DNA binding transcription factor activity Source: TAIR
  • protein dimerization activity Source: InterPro
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Flowering, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Floral homeotic protein PISTILLATA
Alternative name(s):
Transcription factor PI
Gene namesi
Name:PI
Ordered Locus Names:At5g20240
ORF Names:F5O24.130
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G20240
TAIRilocus:2149264 AT5G20240

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001994821 – 208Floral homeotic protein PISTILLATAAdd BLAST208

Proteomic databases

PaxDbiP48007

Expressioni

Inductioni

Positively regulated by the meristem identity proteins APETALA1 and LEAFY with the cooperation of UFO. Repressed by silencing mediated by polycomb group (PcG) protein complex containing EMF1 and EMF2.2 Publications

Gene expression databases

ExpressionAtlasiP48007 baseline and differential
GenevisibleiP48007 AT

Interactioni

Subunit structurei

Forms a heterodimer with APETALA3, capable of binding to CArG-box sequences. AP3/PI heterodimer binds AP1 or SEP3 to form a ternary complex.

Binary interactionsi

WithEntry#Exp.IntActNotes
SEP3O224563EBI-1395334,EBI-592020

GO - Molecular functioni

Protein-protein interaction databases

BioGridi17422, 13 interactors
IntActiP48007, 11 interactors
STRINGi3702.AT5G20240.1

Structurei

3D structure databases

ProteinModelPortaliP48007
SMRiP48007
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 57MADS-boxPROSITE-ProRule annotationAdd BLAST55
Domaini84 – 170K-boxPROSITE-ProRule annotationAdd BLAST87

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili75 – 117Sequence analysisAdd BLAST43

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0014 Eukaryota
COG5068 LUCA
InParanoidiP48007
KOiK09264
OMAiLAFRVHQ
OrthoDBiEOG09360M2K
PhylomeDBiP48007

Family and domain databases

CDDicd00265 MADS_MEF2_like, 1 hit
Gene3Di3.40.1810.10, 1 hit
InterProiView protein in InterPro
IPR033896 MADS_MEF2-like
IPR002487 TF_Kbox
IPR002100 TF_MADSbox
IPR036879 TF_MADSbox_sf
PfamiView protein in Pfam
PF01486 K-box, 1 hit
PF00319 SRF-TF, 1 hit
PRINTSiPR00404 MADSDOMAIN
SMARTiView protein in SMART
SM00432 MADS, 1 hit
SUPFAMiSSF55455 SSF55455, 1 hit
PROSITEiView protein in PROSITE
PS51297 K_BOX, 1 hit
PS00350 MADS_BOX_1, 1 hit
PS50066 MADS_BOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

P48007-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRGKIEIKR IENANNRVVT FSKRRNGLVK KAKEITVLCD AKVALIIFAS
60 70 80 90 100
NGKMIDYCCP SMDLGAMLDQ YQKLSGKKLW DAKHENLSNE IDRIKKENDS
110 120 130 140 150
LQLELRHLKG EDIQSLNLKN LMAVEHAIEH GLDKVRDHQM EILISKRRNE
160 170 180 190 200
KMMAEEQRQL TFQLQQQEMA IASNARGMMM RDHDGQFGYR VQPIQPNLQE

KIMSLVID
Length:208
Mass (Da):24,047
Last modified:February 1, 1996 - v1
Checksum:i49728735AC883CCA
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti29V → A in strain: cv. Cvi-0. 1 Publication1
Natural varianti38L → P in strain: cv. Co-1. 1 Publication1
Natural varianti51N → D in strain: cv. Lisse. 1 Publication1
Natural varianti73K → M in strain: cv. Co-1. 1 Publication1
Natural varianti75S → P in strain: cv. Kent. 1 Publication1
Natural varianti89N → D in strain: cv. Gr-3. 1 Publication1
Natural varianti112D → G in strain: cv. Lisse. 1 Publication1
Natural varianti125E → G in strain: cv. Gr-3. 1 Publication1
Natural varianti140M → I in strain: cv. Corsacalla-1. 1 Publication1
Natural varianti142I → V in strain: cv. Kent. 1 Publication1
Natural varianti160L → I in strain: cv. Kent. 1 Publication1
Natural varianti203M → T in strain: cv. Cvi-0. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30807 mRNA Translation: BAA06465.1
AB035137 Genomic DNA Translation: BAA87000.1
AF115815 Genomic DNA Translation: AAD51984.1
AF115816 Genomic DNA Translation: AAD51985.1
AF115817 Genomic DNA Translation: AAD51986.1
AF115818 Genomic DNA Translation: AAD51987.1
AF115819 Genomic DNA Translation: AAD51988.1
AF115820 Genomic DNA Translation: AAD51989.1
AF115821 Genomic DNA Translation: AAD51990.1
AF115822 Genomic DNA Translation: AAD51991.1
AF115823 Genomic DNA Translation: AAD51992.1
AF115824 Genomic DNA Translation: AAD51993.1
AF115825 Genomic DNA Translation: AAD51994.1
AF115826 Genomic DNA Translation: AAD51995.1
AF115827 Genomic DNA Translation: AAD51996.1
AF115828 Genomic DNA Translation: AAD51997.1
AF115829 Genomic DNA Translation: AAD51998.1
AF115830 Genomic DNA Translation: AAD51999.1
AF296825 Genomic DNA No translation available.
CP002688 Genomic DNA Translation: AED92817.1
PIRiA53839
RefSeqiNP_197524.1, NM_122031.4
UniGeneiAt.21403

Genome annotation databases

EnsemblPlantsiAT5G20240.1; AT5G20240.1; AT5G20240
GeneIDi832146
GrameneiAT5G20240.1; AT5G20240.1; AT5G20240
KEGGiath:AT5G20240

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPIST_ARATH
AccessioniPrimary (citable) accession number: P48007
Secondary accession number(s): Q9SQ07
, Q9SQ08, Q9SQ09, Q9SQ10, Q9SQ11, Q9SQ12, Q9SQ13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 25, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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