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Protein

Floral homeotic protein PISTILLATA

Gene

PI

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcription factor involved in the genetic control of flower development. Is required for normal development of petals and stamens in the wild-type flower. Forms a heterodimer with APETALA3 that is required for autoregulation of both AP3 and PI genes. AP3/PI heterodimer interacts with APETALA1 or SEPALLATA3 to form a ternary complex that could be responsible for the regulation of the genes involved in the flower development. AP3/PI heterodimer activates the expression of NAP.2 Publications

GO - Molecular functioni

  • DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • specification of floral organ identity Source: CACAO
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Flowering, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Floral homeotic protein PISTILLATA
Alternative name(s):
Transcription factor PI
Gene namesi
Name:PI
Ordered Locus Names:At5g20240
ORF Names:F5O24.130
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G20240.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 208208Floral homeotic protein PISTILLATAPRO_0000199482Add
BLAST

Proteomic databases

PaxDbiP48007.

Expressioni

Inductioni

Positively regulated by the meristem identity proteins APETALA1 and LEAFY with the cooperation of UFO. Repressed by silencing mediated by polycomb group (PcG) protein complex containing EMF1 and EMF2.2 Publications

Gene expression databases

GenevisibleiP48007. AT.

Interactioni

Subunit structurei

Forms a heterodimer with APETALA3, capable of binding to CArG-box sequences. AP3/PI heterodimer binds AP1 or SEP3 to form a ternary complex.

Binary interactionsi

WithEntry#Exp.IntActNotes
SEP3O224563EBI-1395334,EBI-592020

Protein-protein interaction databases

BioGridi17422. 13 interactions.
IntActiP48007. 4 interactions.
MINTiMINT-8070775.
STRINGi3702.AT5G20240.1.

Structurei

3D structure databases

ProteinModelPortaliP48007.
SMRiP48007. Positions 2-63.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 5755MADS-boxPROSITE-ProRule annotationAdd
BLAST
Domaini84 – 17087K-boxPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili75 – 11743Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 K-box domain.PROSITE-ProRule annotation
Contains 1 MADS-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
InParanoidiP48007.
KOiK09264.
OMAiEHKMLAF.
OrthoDBiEOG09360M2K.
PhylomeDBiP48007.

Family and domain databases

InterProiIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48007-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRGKIEIKR IENANNRVVT FSKRRNGLVK KAKEITVLCD AKVALIIFAS
60 70 80 90 100
NGKMIDYCCP SMDLGAMLDQ YQKLSGKKLW DAKHENLSNE IDRIKKENDS
110 120 130 140 150
LQLELRHLKG EDIQSLNLKN LMAVEHAIEH GLDKVRDHQM EILISKRRNE
160 170 180 190 200
KMMAEEQRQL TFQLQQQEMA IASNARGMMM RDHDGQFGYR VQPIQPNLQE

KIMSLVID
Length:208
Mass (Da):24,047
Last modified:February 1, 1996 - v1
Checksum:i49728735AC883CCA
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti29 – 291V → A in strain: cv. Cvi-0. 1 Publication
Natural varianti38 – 381L → P in strain: cv. Co-1. 1 Publication
Natural varianti51 – 511N → D in strain: cv. Lisse. 1 Publication
Natural varianti73 – 731K → M in strain: cv. Co-1. 1 Publication
Natural varianti75 – 751S → P in strain: cv. Kent. 1 Publication
Natural varianti89 – 891N → D in strain: cv. Gr-3. 1 Publication
Natural varianti112 – 1121D → G in strain: cv. Lisse. 1 Publication
Natural varianti125 – 1251E → G in strain: cv. Gr-3. 1 Publication
Natural varianti140 – 1401M → I in strain: cv. Corsacalla-1. 1 Publication
Natural varianti142 – 1421I → V in strain: cv. Kent. 1 Publication
Natural varianti160 – 1601L → I in strain: cv. Kent. 1 Publication
Natural varianti203 – 2031M → T in strain: cv. Cvi-0. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30807 mRNA. Translation: BAA06465.1.
AB035137 Genomic DNA. Translation: BAA87000.1.
AF115815 Genomic DNA. Translation: AAD51984.1.
AF115816 Genomic DNA. Translation: AAD51985.1.
AF115817 Genomic DNA. Translation: AAD51986.1.
AF115818 Genomic DNA. Translation: AAD51987.1.
AF115819 Genomic DNA. Translation: AAD51988.1.
AF115820 Genomic DNA. Translation: AAD51989.1.
AF115821 Genomic DNA. Translation: AAD51990.1.
AF115822 Genomic DNA. Translation: AAD51991.1.
AF115823 Genomic DNA. Translation: AAD51992.1.
AF115824 Genomic DNA. Translation: AAD51993.1.
AF115825 Genomic DNA. Translation: AAD51994.1.
AF115826 Genomic DNA. Translation: AAD51995.1.
AF115827 Genomic DNA. Translation: AAD51996.1.
AF115828 Genomic DNA. Translation: AAD51997.1.
AF115829 Genomic DNA. Translation: AAD51998.1.
AF115830 Genomic DNA. Translation: AAD51999.1.
AF296825 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92817.1.
PIRiA53839.
RefSeqiNP_197524.1. NM_122031.3.
UniGeneiAt.21403.

Genome annotation databases

EnsemblPlantsiAT5G20240.1; AT5G20240.1; AT5G20240.
GeneIDi832146.
GrameneiAT5G20240.1; AT5G20240.1; AT5G20240.
KEGGiath:AT5G20240.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30807 mRNA. Translation: BAA06465.1.
AB035137 Genomic DNA. Translation: BAA87000.1.
AF115815 Genomic DNA. Translation: AAD51984.1.
AF115816 Genomic DNA. Translation: AAD51985.1.
AF115817 Genomic DNA. Translation: AAD51986.1.
AF115818 Genomic DNA. Translation: AAD51987.1.
AF115819 Genomic DNA. Translation: AAD51988.1.
AF115820 Genomic DNA. Translation: AAD51989.1.
AF115821 Genomic DNA. Translation: AAD51990.1.
AF115822 Genomic DNA. Translation: AAD51991.1.
AF115823 Genomic DNA. Translation: AAD51992.1.
AF115824 Genomic DNA. Translation: AAD51993.1.
AF115825 Genomic DNA. Translation: AAD51994.1.
AF115826 Genomic DNA. Translation: AAD51995.1.
AF115827 Genomic DNA. Translation: AAD51996.1.
AF115828 Genomic DNA. Translation: AAD51997.1.
AF115829 Genomic DNA. Translation: AAD51998.1.
AF115830 Genomic DNA. Translation: AAD51999.1.
AF296825 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92817.1.
PIRiA53839.
RefSeqiNP_197524.1. NM_122031.3.
UniGeneiAt.21403.

3D structure databases

ProteinModelPortaliP48007.
SMRiP48007. Positions 2-63.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17422. 13 interactions.
IntActiP48007. 4 interactions.
MINTiMINT-8070775.
STRINGi3702.AT5G20240.1.

Proteomic databases

PaxDbiP48007.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G20240.1; AT5G20240.1; AT5G20240.
GeneIDi832146.
GrameneiAT5G20240.1; AT5G20240.1; AT5G20240.
KEGGiath:AT5G20240.

Organism-specific databases

TAIRiAT5G20240.

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
InParanoidiP48007.
KOiK09264.
OMAiEHKMLAF.
OrthoDBiEOG09360M2K.
PhylomeDBiP48007.

Miscellaneous databases

PROiP48007.

Gene expression databases

GenevisibleiP48007. AT.

Family and domain databases

InterProiIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIST_ARATH
AccessioniPrimary (citable) accession number: P48007
Secondary accession number(s): Q9SQ07
, Q9SQ08, Q9SQ09, Q9SQ10, Q9SQ11, Q9SQ12, Q9SQ13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 7, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Mutations in PI cause transformation of petals into sepals and stamina into carpels.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.