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Protein

Elongation factor 1-delta 1

Gene

At1g30230

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

ReactomeiR-ATH-156842. Eukaryotic Translation Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-delta 1
Short name:
EF-1-delta 1
Alternative name(s):
Elongation factor 1B-beta 1
eEF-1B beta 1
Gene namesi
Ordered Locus Names:At1g30230
ORF Names:F12P21.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G30230.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 231230Elongation factor 1-delta 1PRO_0000155035Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP48006.
PRIDEiP48006.

PTM databases

iPTMnetiP48006.

Expressioni

Gene expression databases

ExpressionAtlasiP48006. baseline.
GenevisibleiP48006. AT.

Interactioni

Subunit structurei

EF-1 is composed of 4 subunits: alpha, beta (1B-alpha=beta'), delta (1B-beta), and gamma (1B-gamma).By similarity

Protein-protein interaction databases

BioGridi25138. 3 interactions.
STRINGi3702.AT1G30230.2.

Structurei

3D structure databases

ProteinModelPortaliP48006.
SMRiP48006. Positions 141-231.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 7364GST C-terminalAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi106 – 1116Poly-Asp

Sequence similaritiesi

Belongs to the EF-1-beta/EF-1-delta family.Curated
Contains 1 GST C-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG1668. Eukaryota.
COG2092. LUCA.
HOGENOMiHOG000207273.
InParanoidiP48006.
PhylomeDBiP48006.

Family and domain databases

Gene3Di3.30.70.60. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR014717. Transl_elong_EF1B/ribosomal_S6.
IPR001326. Transl_elong_EF1B_B/D_CS.
IPR014038. Transl_elong_fac_EF1B_bsu/dsu.
[Graphical view]
PfamiPF00736. EF1_GNE. 1 hit.
[Graphical view]
SMARTiSM00888. EF1_GNE. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF54984. SSF54984. 1 hit.
PROSITEiPS00824. EF1BD_1. 1 hit.
PS00825. EF1BD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P48006-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAFPNLNSD AGLKKLDEHL LTRSYITGYQ ASKDDITVFA ALAKPPTSQY
60 70 80 90 100
VNASRWYNHI DALLRISGVS AEGSGVIVEG SAPITEEAVA TPPAADSKDA
110 120 130 140 150
AADEEDDDDV DLFGEETEEE KKAAEERAAS VKASTKKKES GKSSVLIDIK
160 170 180 190 200
PWDDETDMKK LEEAVKSIQM EGLFWGASKL VPVGYGIKKL QILCTIVDDL
210 220 230
VSIDTMIEEQ LTVEPINEYV QSCDIVAFNK I
Length:231
Mass (Da):25,132
Last modified:February 21, 2001 - v2
Checksum:i2A928F477BF9E932
GO

Sequence cautioni

The sequence AAG50564.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti102 – 1021A → P in CAA52751 (PubMed:8666245).Curated
Sequence conflicti115 – 1151E → Q in CAA52751 (PubMed:8666245).Curated
Sequence conflicti161 – 1611L → R in CAA52751 (PubMed:8666245).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74733 Genomic DNA. Translation: CAA52751.1.
AC073506 Genomic DNA. Translation: AAG50564.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE31195.1.
AK226216 mRNA. Translation: BAE98381.1.
PIRiS37103.
RefSeqiNP_174314.2. NM_102762.4. [P48006-1]
UniGeneiAt.24745.

Genome annotation databases

EnsemblPlantsiAT1G30230.1; AT1G30230.1; AT1G30230. [P48006-1]
GeneIDi839903.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74733 Genomic DNA. Translation: CAA52751.1.
AC073506 Genomic DNA. Translation: AAG50564.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE31195.1.
AK226216 mRNA. Translation: BAE98381.1.
PIRiS37103.
RefSeqiNP_174314.2. NM_102762.4. [P48006-1]
UniGeneiAt.24745.

3D structure databases

ProteinModelPortaliP48006.
SMRiP48006. Positions 141-231.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi25138. 3 interactions.
STRINGi3702.AT1G30230.2.

PTM databases

iPTMnetiP48006.

Proteomic databases

PaxDbiP48006.
PRIDEiP48006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G30230.1; AT1G30230.1; AT1G30230. [P48006-1]
GeneIDi839903.

Organism-specific databases

TAIRiAT1G30230.

Phylogenomic databases

eggNOGiKOG1668. Eukaryota.
COG2092. LUCA.
HOGENOMiHOG000207273.
InParanoidiP48006.
PhylomeDBiP48006.

Enzyme and pathway databases

ReactomeiR-ATH-156842. Eukaryotic Translation Elongation.

Miscellaneous databases

PROiP48006.

Gene expression databases

ExpressionAtlasiP48006. baseline.
GenevisibleiP48006. AT.

Family and domain databases

Gene3Di3.30.70.60. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR014717. Transl_elong_EF1B/ribosomal_S6.
IPR001326. Transl_elong_EF1B_B/D_CS.
IPR014038. Transl_elong_fac_EF1B_bsu/dsu.
[Graphical view]
PfamiPF00736. EF1_GNE. 1 hit.
[Graphical view]
SMARTiSM00888. EF1_GNE. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF54984. SSF54984. 1 hit.
PROSITEiPS00824. EF1BD_1. 1 hit.
PS00825. EF1BD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The first intron of the Arabidopsis thaliana gene coding for elongation factor 1 beta contains an enhancer-like element."
    Gidekel M., Jimenez B., Herrera-Estrella L.
    Gene 170:201-206(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
    Tissue: Leaf.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  6. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiEF1D1_ARATH
AccessioniPrimary (citable) accession number: P48006
Secondary accession number(s): Q0WWW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 21, 2001
Last modified: January 20, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.