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P48004

- PSA7_RAT

UniProt

P48004 - PSA7_RAT

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Protein

Proteasome subunit alpha type-7

Gene

Psma7

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions (By similarity). Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response (By similarity).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Protein family/group databases

MEROPSiT01.979.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-7 (EC:3.4.25.1)
Alternative name(s):
Proteasome subunit RC6-1
Gene namesi
Name:Psma7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi61851. Psma7.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
  3. proteasome core complex Source: UniProtKB
  4. proteasome core complex, alpha-subunit complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 254254Proteasome subunit alpha type-7PRO_0000124144Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi136 – 1361O-linked (GlcNAc)By similarity
Modified residuei159 – 1591PhosphotyrosineBy similarity
Modified residuei233 – 2331N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP48004.
PRIDEiP48004.

PTM databases

PhosphoSiteiP48004.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

GenevestigatoriP48004.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. PSMA7 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly. Interacts with HIF1A (By similarity). Interacts with RAB7A (By similarity). Interacts with PARK2 (By similarity). Interacts with MAVS (By similarity).By similarity

Protein-protein interaction databases

IntActiP48004. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP48004.
SMRiP48004. Positions 2-238.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000091085.
HOVERGENiHBG003005.
InParanoidiP48004.
PhylomeDBiP48004.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform RC6-IL (identifier: P48004-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSYDRAITVF SPDGHLFQVE YAQEAVKKGS TAVGVRGRDI VVLGVEKKSV
60 70 80 90 100
AKLQDERTVR KICALDDNVC MAFAVVASVS GLTADARIVI NRARVECQSH
110 120 130 140 150
RLTVGDPVTV EYITRYIASL KQRYTQSNGR RPFGISALIV GFDFDGTPRL
160 170 180 190 200
YQTDPSGTYH AWKANAIGRG AKSVREFLEK NYTDDAIETD DLTIKLVIKA
210 220 230 240 250
LLEVVQSGGK NIELAVMRRD QPLKILSPEE IEKYVAEIEK EKEENEKKKQ

KKAS
Length:254
Mass (Da):28,326
Last modified:February 1, 1996 - v1
Checksum:iC454A63F59B5FA8D
GO
Isoform RC6-IS (identifier: P48004-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     75-80: Missing.

Show »
Length:248
Mass (Da):27,784
Checksum:iAE4B4DA4EDC2C8E0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701C → V AA sequence (PubMed:7578256)Curated
Sequence conflicti105 – 1051G → E AA sequence (PubMed:7578256)Curated
Sequence conflicti114 – 1141T → V AA sequence (PubMed:7578256)Curated
Sequence conflicti154 – 1541D → N AA sequence (PubMed:7578256)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei75 – 806Missing in isoform RC6-IS. 1 PublicationVSP_005284

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D30804 mRNA. Translation: BAA06463.1.
PIRiS60038.
UniGeneiRn.105784.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D30804 mRNA. Translation: BAA06463.1 .
PIRi S60038.
UniGenei Rn.105784.

3D structure databases

ProteinModelPortali P48004.
SMRi P48004. Positions 2-238.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P48004. 2 interactions.

Protein family/group databases

MEROPSi T01.979.

PTM databases

PhosphoSitei P48004.

Proteomic databases

PaxDbi P48004.
PRIDEi P48004.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

RGDi 61851. Psma7.

Phylogenomic databases

eggNOGi COG0638.
HOGENOMi HOG000091085.
HOVERGENi HBG003005.
InParanoidi P48004.
PhylomeDBi P48004.

Miscellaneous databases

PROi P48004.

Gene expression databases

Genevestigatori P48004.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
InterProi IPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view ]
SMARTi SM00948. Proteasome_A_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of two types of cDNA encoding subunit RC6-I of rat proteasomes."
    Ni R., Tomita Y., Tokunaga F., Liang T.J., Noda C., Ichihara A., Tanaka K.
    Biochim. Biophys. Acta 1264:45-52(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS RC6-IL AND RC6-IS), PARTIAL PROTEIN SEQUENCE.
    Strain: Wistar.
    Tissue: Liver.

Entry informationi

Entry nameiPSA7_RAT
AccessioniPrimary (citable) accession number: P48004
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3