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P48004

- PSA7_RAT

UniProt

P48004 - PSA7_RAT

Protein

Proteasome subunit alpha type-7

Gene

Psma7

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions By similarity. Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response By similarity.By similarity

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Protein family/group databases

    MEROPSiT01.979.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit alpha type-7 (EC:3.4.25.1)
    Alternative name(s):
    Proteasome subunit RC6-1
    Gene namesi
    Name:Psma7
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi61851. Psma7.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. proteasome core complex Source: UniProtKB
    4. proteasome core complex, alpha-subunit complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 254254Proteasome subunit alpha type-7PRO_0000124144Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi136 – 1361O-linked (GlcNAc)By similarity
    Modified residuei159 – 1591PhosphotyrosineBy similarity
    Modified residuei233 – 2331N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP48004.
    PRIDEiP48004.

    PTM databases

    PhosphoSiteiP48004.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Gene expression databases

    GenevestigatoriP48004.

    Interactioni

    Subunit structurei

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. PSMA7 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly. Interacts with HIF1A By similarity. Interacts with RAB7A By similarity. Interacts with PARK2 By similarity. Interacts with MAVS By similarity.By similarity

    Protein-protein interaction databases

    IntActiP48004. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP48004.
    SMRiP48004. Positions 2-238.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1A family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    HOGENOMiHOG000091085.
    HOVERGENiHBG003005.
    InParanoidiP48004.
    PhylomeDBiP48004.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR000426. Proteasome_asu_N.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    PF10584. Proteasome_A_N. 1 hit.
    [Graphical view]
    SMARTiSM00948. Proteasome_A_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
    PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform RC6-IL (identifier: P48004-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSYDRAITVF SPDGHLFQVE YAQEAVKKGS TAVGVRGRDI VVLGVEKKSV    50
    AKLQDERTVR KICALDDNVC MAFAVVASVS GLTADARIVI NRARVECQSH 100
    RLTVGDPVTV EYITRYIASL KQRYTQSNGR RPFGISALIV GFDFDGTPRL 150
    YQTDPSGTYH AWKANAIGRG AKSVREFLEK NYTDDAIETD DLTIKLVIKA 200
    LLEVVQSGGK NIELAVMRRD QPLKILSPEE IEKYVAEIEK EKEENEKKKQ 250
    KKAS 254
    Length:254
    Mass (Da):28,326
    Last modified:February 1, 1996 - v1
    Checksum:iC454A63F59B5FA8D
    GO
    Isoform RC6-IS (identifier: P48004-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         75-80: Missing.

    Show »
    Length:248
    Mass (Da):27,784
    Checksum:iAE4B4DA4EDC2C8E0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti70 – 701C → V AA sequence (PubMed:7578256)Curated
    Sequence conflicti105 – 1051G → E AA sequence (PubMed:7578256)Curated
    Sequence conflicti114 – 1141T → V AA sequence (PubMed:7578256)Curated
    Sequence conflicti154 – 1541D → N AA sequence (PubMed:7578256)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei75 – 806Missing in isoform RC6-IS. 1 PublicationVSP_005284

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D30804 mRNA. Translation: BAA06463.1.
    PIRiS60038.
    UniGeneiRn.105784.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D30804 mRNA. Translation: BAA06463.1 .
    PIRi S60038.
    UniGenei Rn.105784.

    3D structure databases

    ProteinModelPortali P48004.
    SMRi P48004. Positions 2-238.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P48004. 2 interactions.

    Protein family/group databases

    MEROPSi T01.979.

    PTM databases

    PhosphoSitei P48004.

    Proteomic databases

    PaxDbi P48004.
    PRIDEi P48004.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    RGDi 61851. Psma7.

    Phylogenomic databases

    eggNOGi COG0638.
    HOGENOMi HOG000091085.
    HOVERGENi HBG003005.
    InParanoidi P48004.
    PhylomeDBi P48004.

    Miscellaneous databases

    PROi P48004.

    Gene expression databases

    Genevestigatori P48004.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR000426. Proteasome_asu_N.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    PF10584. Proteasome_A_N. 1 hit.
    [Graphical view ]
    SMARTi SM00948. Proteasome_A_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS00388. PROTEASOME_ALPHA_1. 1 hit.
    PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of two types of cDNA encoding subunit RC6-I of rat proteasomes."
      Ni R., Tomita Y., Tokunaga F., Liang T.J., Noda C., Ichihara A., Tanaka K.
      Biochim. Biophys. Acta 1264:45-52(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS RC6-IL AND RC6-IS), PARTIAL PROTEIN SEQUENCE.
      Strain: Wistar.
      Tissue: Liver.

    Entry informationi

    Entry nameiPSA7_RAT
    AccessioniPrimary (citable) accession number: P48004
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3