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P48004 (PSA7_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit alpha type-7

EC=3.4.25.1
Alternative name(s):
Proteasome subunit RC6-1
Gene names
Name:Psma7
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions By similarity. Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response By similarity.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. PSMA7 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly. Interacts with HIF1A By similarity. Interacts with RAB7A By similarity. Interacts with PARK2 By similarity. Interacts with MAVS By similarity.

Subcellular location

Cytoplasm. Nucleus.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the peptidase T1A family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform RC6-IL (identifier: P48004-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform RC6-IS (identifier: P48004-2)

The sequence of this isoform differs from the canonical sequence as follows:
     75-80: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 254254Proteasome subunit alpha type-7
PRO_0000124144

Amino acid modifications

Modified residue1591Phosphotyrosine By similarity
Modified residue2331N6-acetyllysine By similarity
Glycosylation1361O-linked (GlcNAc) By similarity

Natural variations

Alternative sequence75 – 806Missing in isoform RC6-IS.
VSP_005284

Experimental info

Sequence conflict701C → V AA sequence Ref.1
Sequence conflict1051G → E AA sequence Ref.1
Sequence conflict1141T → V AA sequence Ref.1
Sequence conflict1541D → N AA sequence Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform RC6-IL [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: C454A63F59B5FA8D

FASTA25428,326
        10         20         30         40         50         60 
MSYDRAITVF SPDGHLFQVE YAQEAVKKGS TAVGVRGRDI VVLGVEKKSV AKLQDERTVR 

        70         80         90        100        110        120 
KICALDDNVC MAFAVVASVS GLTADARIVI NRARVECQSH RLTVGDPVTV EYITRYIASL 

       130        140        150        160        170        180 
KQRYTQSNGR RPFGISALIV GFDFDGTPRL YQTDPSGTYH AWKANAIGRG AKSVREFLEK 

       190        200        210        220        230        240 
NYTDDAIETD DLTIKLVIKA LLEVVQSGGK NIELAVMRRD QPLKILSPEE IEKYVAEIEK 

       250 
EKEENEKKKQ KKAS 

« Hide

Isoform RC6-IS [UniParc].

Checksum: AE4B4DA4EDC2C8E0
Show »

FASTA24827,784

References

[1]"Molecular cloning of two types of cDNA encoding subunit RC6-I of rat proteasomes."
Ni R., Tomita Y., Tokunaga F., Liang T.J., Noda C., Ichihara A., Tanaka K.
Biochim. Biophys. Acta 1264:45-52(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS RC6-IL AND RC6-IS), PARTIAL PROTEIN SEQUENCE.
Strain: Wistar.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D30804 mRNA. Translation: BAA06463.1.
PIRS60038.
UniGeneRn.105784.

3D structure databases

ProteinModelPortalP48004.
SMRP48004. Positions 2-238.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP48004. 2 interactions.

Protein family/group databases

MEROPST01.979.

PTM databases

PhosphoSiteP48004.

Proteomic databases

PaxDbP48004.
PRIDEP48004.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

RGD61851. Psma7.

Phylogenomic databases

eggNOGCOG0638.
HOGENOMHOG000091085.
HOVERGENHBG003005.
InParanoidP48004.
PhylomeDBP48004.

Gene expression databases

GenevestigatorP48004.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP48004.

Entry information

Entry namePSA7_RAT
AccessionPrimary (citable) accession number: P48004
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries