Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Proteasome subunit alpha type-7

Gene

Psma7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions (By similarity). Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response (By similarity).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Protein family/group databases

MEROPSiT01.979.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-7 (EC:3.4.25.1)
Alternative name(s):
Proteasome subunit RC6-1
Gene namesi
Name:Psma7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61851. Psma7.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 254254Proteasome subunit alpha type-7PRO_0000124144Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi136 – 1361O-linked (GlcNAc)By similarity
Modified residuei159 – 1591PhosphotyrosineBy similarity
Modified residuei233 – 2331N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP48004.
PRIDEiP48004.

PTM databases

PhosphoSiteiP48004.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

GenevestigatoriP48004.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. PSMA7 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly. Interacts with HIF1A (By similarity). Interacts with RAB7A (By similarity). Interacts with PARK2 (By similarity). Interacts with MAVS (By similarity).By similarity

Protein-protein interaction databases

IntActiP48004. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP48004.
SMRiP48004. Positions 2-238.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000091085.
HOVERGENiHBG003005.
InParanoidiP48004.
PhylomeDBiP48004.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform RC6-IL (identifier: P48004-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSYDRAITVF SPDGHLFQVE YAQEAVKKGS TAVGVRGRDI VVLGVEKKSV
60 70 80 90 100
AKLQDERTVR KICALDDNVC MAFAVVASVS GLTADARIVI NRARVECQSH
110 120 130 140 150
RLTVGDPVTV EYITRYIASL KQRYTQSNGR RPFGISALIV GFDFDGTPRL
160 170 180 190 200
YQTDPSGTYH AWKANAIGRG AKSVREFLEK NYTDDAIETD DLTIKLVIKA
210 220 230 240 250
LLEVVQSGGK NIELAVMRRD QPLKILSPEE IEKYVAEIEK EKEENEKKKQ

KKAS
Length:254
Mass (Da):28,326
Last modified:February 1, 1996 - v1
Checksum:iC454A63F59B5FA8D
GO
Isoform RC6-IS (identifier: P48004-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     75-80: Missing.

Show »
Length:248
Mass (Da):27,784
Checksum:iAE4B4DA4EDC2C8E0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701C → V AA sequence (PubMed:7578256).Curated
Sequence conflicti105 – 1051G → E AA sequence (PubMed:7578256).Curated
Sequence conflicti114 – 1141T → V AA sequence (PubMed:7578256).Curated
Sequence conflicti154 – 1541D → N AA sequence (PubMed:7578256).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei75 – 806Missing in isoform RC6-IS. 1 PublicationVSP_005284

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30804 mRNA. Translation: BAA06463.1.
PIRiS60038.
UniGeneiRn.105784.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30804 mRNA. Translation: BAA06463.1.
PIRiS60038.
UniGeneiRn.105784.

3D structure databases

ProteinModelPortaliP48004.
SMRiP48004. Positions 2-238.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP48004. 2 interactions.

Protein family/group databases

MEROPSiT01.979.

PTM databases

PhosphoSiteiP48004.

Proteomic databases

PaxDbiP48004.
PRIDEiP48004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi61851. Psma7.

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000091085.
HOVERGENiHBG003005.
InParanoidiP48004.
PhylomeDBiP48004.

Miscellaneous databases

PROiP48004.

Gene expression databases

GenevestigatoriP48004.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of two types of cDNA encoding subunit RC6-I of rat proteasomes."
    Ni R., Tomita Y., Tokunaga F., Liang T.J., Noda C., Ichihara A., Tanaka K.
    Biochim. Biophys. Acta 1264:45-52(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS RC6-IL AND RC6-IS), PARTIAL PROTEIN SEQUENCE.
    Strain: Wistar.
    Tissue: Liver.

Entry informationi

Entry nameiPSA7_RAT
AccessioniPrimary (citable) accession number: P48004
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 27, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.