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Protein

Homeobox protein knotted-1-like 3

Gene

KNAT3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi343 – 40664Homeobox; TALE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • cellular response to cytokine stimulus Source: TAIR
  • detection of cytokinin stimulus Source: TAIR
  • response to light stimulus Source: TAIR
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein knotted-1-like 3
Alternative name(s):
Protein KNAT3
Gene namesi
Name:KNAT3
Ordered Locus Names:At5g25220
ORF Names:F21J6.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G25220.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 431431Homeobox protein knotted-1-like 3PRO_0000048959Add
BLAST

Proteomic databases

PaxDbiP48000.
PRIDEiP48000.

PTM databases

iPTMnetiP48000.

Expressioni

Gene expression databases

ExpressionAtlasiP48000. baseline and differential.
GenevisibleiP48000. AT.

Interactioni

Subunit structurei

May form heterodimeric complex with the TALE/BELL proteins (By similarity). Interacts with OFP1, OFP2, OFP4, OFP12 and OFP14 (PubMed:15781858). Interacts with KNATM-B (PubMed:18398054).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BEL1Q388973EBI-1153908,EBI-1153783

Protein-protein interaction databases

BioGridi17868. 25 interactions.
IntActiP48000. 18 interactions.
STRINGi3702.AT5G25220.1.

Structurei

3D structure databases

ProteinModelPortaliP48000.
SMRiP48000. Positions 352-401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini322 – 34221ELKPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi23 – 3311Poly-ProAdd
BLAST
Compositional biasi34 – 374Poly-Gln
Compositional biasi58 – 614Poly-Asn
Compositional biasi84 – 907Poly-Ala
Compositional biasi104 – 1085Poly-Asn

Sequence similaritiesi

Belongs to the TALE/KNOX homeobox family.PROSITE-ProRule annotation
Contains 1 ELK domain.PROSITE-ProRule annotation
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0773. Eukaryota.
ENOG410XPMQ. LUCA.
HOGENOMiHOG000272741.
InParanoidiP48000.
OMAiSWQNARH.
PhylomeDBiP48000.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR005539. ELK_dom.
IPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
IPR005540. KNOX1.
IPR005541. KNOX2.
[Graphical view]
PfamiPF03789. ELK. 1 hit.
PF05920. Homeobox_KN. 1 hit.
PF03790. KNOX1. 1 hit.
PF03791. KNOX2. 1 hit.
[Graphical view]
SMARTiSM01188. ELK. 1 hit.
SM00389. HOX. 1 hit.
SM01255. KNOX1. 1 hit.
SM01256. KNOX2. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS51213. ELK. 1 hit.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P48000-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFHHNHLSQ DLSFNHFTDQ HQPPPPQPPP PPPQQQQHFQ EAPPPNWLNT
60 70 80 90 100
ALLRSSDNNN NFLNLHTATA NTTTASSSDS PSSAAAAAAA NQWLSRSSSF
110 120 130 140 150
LQRNNNNNAS IVGDGIDDVT GGADTMIQGE MKTGGGENKN DGGGATAADG
160 170 180 190 200
VVSWQNARHK AEILSHPLYE QLLSAHVACL RIATPVDQLP RIDAQLAQSQ
210 220 230 240 250
HVVAKYSALG AAAQGLVGDD KELDQFMTHY VLLLCSFKEQ LQQHVRVHAM
260 270 280 290 300
EAVMACWEIE QSLQSLTGVS PGEGMGATMS DDEDEQVESD ANMFDGGLDV
310 320 330 340 350
LGFGPLIPTE SERSLMERVR QELKHELKQG YKEKIVDIRE EILRKRRAGK
360 370 380 390 400
LPGDTTSVLK AWWQSHSKWP YPTEEDKARL VQETGLQLKQ INNWFINQRK
410 420 430
RNWHSNPSSS TVLKNKRKSN AGDNSGRERF A
Length:431
Mass (Da):47,600
Last modified:February 1, 1996 - v1
Checksum:i5222B67AB54B9673
GO

Sequence cautioni

The sequence AAM53320.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti96 – 961R → L in AAM63298 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92392 mRNA. Translation: CAA63130.1.
AC006259 Genomic DNA. Translation: AAC98441.1.
CP002688 Genomic DNA. Translation: AED93413.1.
AY086091 mRNA. Translation: AAM63298.1.
AY120762 mRNA. Translation: AAM53320.1. Different initiation.
BT000139 mRNA. Translation: AAN15458.1.
RefSeqiNP_197904.1. NM_122431.2. [P48000-1]
UniGeneiAt.5376.

Genome annotation databases

EnsemblPlantsiAT5G25220.1; AT5G25220.1; AT5G25220. [P48000-1]
GeneIDi832593.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92392 mRNA. Translation: CAA63130.1.
AC006259 Genomic DNA. Translation: AAC98441.1.
CP002688 Genomic DNA. Translation: AED93413.1.
AY086091 mRNA. Translation: AAM63298.1.
AY120762 mRNA. Translation: AAM53320.1. Different initiation.
BT000139 mRNA. Translation: AAN15458.1.
RefSeqiNP_197904.1. NM_122431.2. [P48000-1]
UniGeneiAt.5376.

3D structure databases

ProteinModelPortaliP48000.
SMRiP48000. Positions 352-401.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17868. 25 interactions.
IntActiP48000. 18 interactions.
STRINGi3702.AT5G25220.1.

PTM databases

iPTMnetiP48000.

Proteomic databases

PaxDbiP48000.
PRIDEiP48000.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G25220.1; AT5G25220.1; AT5G25220. [P48000-1]
GeneIDi832593.

Organism-specific databases

TAIRiAT5G25220.

Phylogenomic databases

eggNOGiKOG0773. Eukaryota.
ENOG410XPMQ. LUCA.
HOGENOMiHOG000272741.
InParanoidiP48000.
OMAiSWQNARH.
PhylomeDBiP48000.

Miscellaneous databases

PROiP48000.

Gene expression databases

ExpressionAtlasiP48000. baseline and differential.
GenevisibleiP48000. AT.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR005539. ELK_dom.
IPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
IPR005540. KNOX1.
IPR005541. KNOX2.
[Graphical view]
PfamiPF03789. ELK. 1 hit.
PF05920. Homeobox_KN. 1 hit.
PF03790. KNOX1. 1 hit.
PF03791. KNOX2. 1 hit.
[Graphical view]
SMARTiSM01188. ELK. 1 hit.
SM00389. HOX. 1 hit.
SM01255. KNOX1. 1 hit.
SM01256. KNOX2. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS51213. ELK. 1 hit.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Localization of expression of KNAT3, a class 2 knotted1-like gene."
    Serikawa K.A., Martinez-Laborda A., Kim H.S., Zambryski P.C.
    Plant J. 11:853-861(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 17-431 AND 126-431.
    Strain: cv. Columbia.
  6. "A central role of Arabidopsis thaliana ovate family proteins in networking and subcellular localization of 3-aa loop extension homeodomain proteins."
    Hackbusch J., Richter K., Muller J., Salamini F., Uhrig J.F.
    Proc. Natl. Acad. Sci. U.S.A. 102:4908-4912(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH OFP1; OFP2; OFP4; OFP12 AND OFP14.
  7. "KNOX lost the OX: the Arabidopsis KNATM gene defines a novel class of KNOX transcriptional regulators missing the homeodomain."
    Magnani E., Hake S.
    Plant Cell 20:875-887(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KNATM.

Entry informationi

Entry nameiKNAT3_ARATH
AccessioniPrimary (citable) accession number: P48000
Secondary accession number(s): Q8L839, Q8LDC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 11, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.