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Protein

Cysteine synthase, chloroplastic/chromoplastic

Gene

OASB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a major cysteine synthase.2 Publications

Catalytic activityi

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.2 Publications

Cofactori

Kineticsi

  1. KM=0.81 mM for O3-acetyl-L-serine for the cysteine synthase activity2 Publications
  2. KM=0.31 mM for O3-acetyl-L-serine for the cysteine synthase activity2 Publications
  3. KM=3.0 µM for H2S for the cysteine synthase activity2 Publications
  1. Vmax=171 µmol/min/mg enzyme for L-cysteine for the cysteine synthase activity2 Publications
  2. Vmax=592 µmol/min/mg enzyme for L-cysteine for the cysteine synthase activity2 Publications
  3. Vmax=0.37 µmol/min/mg enzyme for H2S for the L-3-cyanoalanine synthase activity2 Publications

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Serine acetyltransferase 3, mitochondrial (SAT3), Serine acetyltransferase 5 (SAT5), Serine acetyltransferase 1, chloroplastic (SAT1), Serine acetyltransferase 2 (SAT2), Serine acetyltransferase 4 (SAT4)
  2. Bifunctional L-3-cyanoalanine synthase/cysteine synthase D2 (CYSD2), Bifunctional cystathionine gamma-lyase/cysteine synthase (DES1), Cysteine synthase, mitochondrial (OASC), Bifunctional L-3-cyanoalanine synthase/cysteine synthase D1 (CYSD1), Cysteine synthase, chloroplastic/chromoplastic (OASB), Cysteine synthase 1 (OASA1)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei147Pyridoxal phosphateBy similarity1
Binding sitei339Pyridoxal phosphateBy similarity1

GO - Molecular functioni

  • cysteine synthase activity Source: TAIR
  • pyridoxal phosphate binding Source: GO_Central

GO - Biological processi

  • cysteine biosynthetic process Source: TAIR
  • cysteine biosynthetic process from serine Source: GO_Central
  • double fertilization forming a zygote and endosperm Source: TAIR
  • pollen tube growth Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to cytokinin Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Cysteine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:AT2G43750-MONOMER
ReactomeiR-ATH-1614603 Cysteine formation from homocysteine
UniPathwayiUPA00136; UER00200

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine synthase, chloroplastic/chromoplastic (EC:2.5.1.47)
Alternative name(s):
At.OAS.7-4
Beta-substituted Ala synthase 2;1
Short name:
ARAth-Bsas2;1
CSase B
Short name:
AtCS-B
Short name:
CS-B
O-acetylserine (thiol)-lyase
O-acetylserine sulfhydrylase
OAS-TL B
cpACS1
Gene namesi
Name:OASB
Ordered Locus Names:At2g43750
ORF Names:F18O19.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G43750
TAIRilocus:2043964 AT2G43750

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Chromoplast, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 60Chloroplast and chromoplastCombined sourcesAdd BLAST60
ChainiPRO_000000634961 – 392Cysteine synthase, chloroplastic/chromoplasticAdd BLAST332

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61N-acetylalanineCombined sources1 Publication1
Modified residuei116N6-(pyridoxal phosphate)lysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP47999
PRIDEiP47999

2D gel databases

SWISS-2DPAGEP47999

PTM databases

iPTMnetiP47999

Expressioni

Gene expression databases

ExpressionAtlasiP47999 baseline and differential
GenevisibleiP47999 AT

Interactioni

Subunit structurei

Homodimer. Component of the cysteine synthase complex (CSC) composed of two OAS-TL dimers and one SAT hexamer (By similarity).By similarity

Protein-protein interaction databases

BioGridi4314, 4 interactors
IntActiP47999, 1 interactor
STRINGi3702.AT2G43750.1

Structurei

3D structure databases

ProteinModelPortaliP47999
SMRiP47999
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni251 – 255Pyridoxal phosphate bindingBy similarity5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi342 – 347Poly-Ala6

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1252 Eukaryota
COG0031 LUCA
HOGENOMiHOG000217394
InParanoidiP47999
KOiK01738
OMAiEKHYETT
OrthoDBiEOG09360FNG
PhylomeDBiP47999

Family and domain databases

InterProiView protein in InterPro
IPR005856 Cys_synth
IPR005859 CysK
IPR001216 P-phosphate_BS
IPR001926 PLP-dep
IPR036052 Trypto_synt_PLP_dependent
PfamiView protein in Pfam
PF00291 PALP, 1 hit
SUPFAMiSSF53686 SSF53686, 1 hit
TIGRFAMsiTIGR01139 cysK, 1 hit
TIGR01136 cysKM, 1 hit
PROSITEiView protein in PROSITE
PS00901 CYS_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47999-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATSSSAFL LNPLTSRHRP FKYSPELSSL SLSSRKAAAF DVSSAAFTLK
60 70 80 90 100
RQSRSDVVCK AVSIKPEAGV EGLNIADNAA QLIGKTPMVY LNNVVKGCVA
110 120 130 140 150
SVAAKLEIME PCCSVKDRIG YSMITDAEEK GLITPGKSVL VESTSGNTGI
160 170 180 190 200
GLAFIAASKG YKLILTMPAS MSLERRVLLR AFGAELVLTE PAKGMTGAIQ
210 220 230 240 250
KAEEILKKTP NSYMLQQFDN PANPKIHYET TGPEIWEDTR GKIDILVAGI
260 270 280 290 300
GTGGTITGVG RFIKERKPEL KVIGVEPTES AILSGGKPGP HKIQGIGAGF
310 320 330 340 350
VPKNLDLAIV DEYIAISSEE AIETSKQLAL QEGLLVGISS GAAAAAAIQV
360 370 380 390
AKRPENAGKL IAVVFPSFGE RYLSTQLFQS IREECEQMQP EL
Length:392
Mass (Da):41,656
Last modified:December 1, 2000 - v2
Checksum:iB1220F01E8E31A12
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti232G → N in CAA57344 (PubMed:7610184).Curated1
Sequence conflicti288P → T in CAA57344 (PubMed:7610184).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80377 mRNA Translation: CAA56594.2
X81698 mRNA Translation: CAA57344.1
AJ271728 Genomic DNA Translation: CAB71292.1
AC002333 Genomic DNA Translation: AAB64031.1
CP002685 Genomic DNA Translation: AEC10317.1
CP002685 Genomic DNA Translation: AEC10318.1
AY065375 mRNA Translation: AAL38816.1
AY096681 mRNA Translation: AAM20315.1
AY086156 mRNA Translation: AAM63361.1
PIRiA84870
S48695
RefSeqiNP_001189745.1, NM_001202816.1
NP_181903.1, NM_129937.4
UniGeneiAt.20491
At.72689

Genome annotation databases

EnsemblPlantsiAT2G43750.1; AT2G43750.1; AT2G43750
AT2G43750.2; AT2G43750.2; AT2G43750
GeneIDi818978
GrameneiAT2G43750.1; AT2G43750.1; AT2G43750
AT2G43750.2; AT2G43750.2; AT2G43750
KEGGiath:AT2G43750

Similar proteinsi

Entry informationi

Entry nameiCYSKP_ARATH
AccessioniPrimary (citable) accession number: P47999
Secondary accession number(s): O22828, Q42568
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 1, 2000
Last modified: May 23, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health