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Protein

Cysteine synthase 1

Gene

OASA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a major cysteine synthase, probably involved in maintaining organic sulfur level.3 Publications

Catalytic activityi

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.2 Publications

Cofactori

pyridoxal 5'-phosphate2 Publications

Enzyme regulationi

Interaction with the sulfate transporter SULTR1;2 enhances its catalytic activity.1 Publication

Kineticsi

  1. KM=1.4 mM for O-acetylserine (at pH 7.0 and 25 degrees Celsius) for the cysteine synthase activity3 Publications
  2. KM=1.22 mM for O(3)-acetyl-L-serine for the cysteine synthase activity3 Publications
  3. KM=0.69 mM for O(3)-acetyl-L-serine for the cysteine synthase activity3 Publications
  4. KM=0.22 mM for Na2S (at pH 7.0 and 25 degrees Celsius) for the cysteine synthase activity3 Publications
  5. KM=5.6 µM for H2S for the cysteine synthase activity3 Publications
  1. Vmax=225 µmol/min/mg enzyme for L-cysteine for the cysteine synthase activity3 Publications
  2. Vmax=906 µmol/min/mg enzyme for L-cysteine for the cysteine synthase activity3 Publications
  3. Vmax=0.43 µmol/min/mg enzyme for H2S for the L-3-cyanoalanine synthase activity3 Publications

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Serine acetyltransferase 3, mitochondrial (SAT3), Serine acetyltransferase 5 (SAT5), Serine acetyltransferase 1, chloroplastic (SAT1), Serine acetyltransferase 2 (SAT2), Serine acetyltransferase 4 (SAT4)
  2. Bifunctional L-3-cyanoalanine synthase/cysteine synthase D2 (CYSD2), Bifunctional cystathionine gamma-lyase/cysteine synthase (DES1), Cysteine synthase, mitochondrial (OASC), Bifunctional L-3-cyanoalanine synthase/cysteine synthase D1 (CYSD1), Cysteine synthase, chloroplastic/chromoplastic (OASB), Cysteine synthase 1 (OASA1)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77Pyridoxal phosphateCombined sources2 Publications1
Binding sitei269Pyridoxal phosphateCombined sources2 Publications1

GO - Molecular functioni

  • cysteine synthase activity Source: TAIR
  • pyridoxal phosphate binding Source: GO_Central
  • transferase activity Source: UniProtKB-KW

GO - Biological processi

  • aging Source: TAIR
  • cellular amino acid biosynthetic process Source: UniProtKB-KW
  • cysteine biosynthetic process Source: TAIR
  • cysteine biosynthetic process from serine Source: TAIR
  • double fertilization forming a zygote and endosperm Source: TAIR
  • pollen tube growth Source: TAIR
  • response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:AT4G14880-MONOMER.
BRENDAi2.5.1.47. 399.
SABIO-RKP47998.
UniPathwayiUPA00136; UER00200.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine synthase 1 (EC:2.5.1.47)
Alternative name(s):
At.OAS.5-8
Beta-substituted Ala synthase 1;1
Short name:
ARAth-Bsas1;1
CSase A
Short name:
AtCS-A
Cys-3A
O-acetylserine (thiol)-lyase 1
Short name:
OAS-TL A
O-acetylserine sulfhydrylase
Protein ONSET OF LEAF DEATH 3
Gene namesi
Name:OASA1
Synonyms:OAS1, OASS, OLD3
Ordered Locus Names:At4g14880
ORF Names:dl3480c
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G14880.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • cytosol Source: TAIR
  • membrane Source: TAIR
  • nucleus Source: TAIR
  • peroxisome Source: TAIR
  • plasma membrane Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype but displays lower levels of thiols in roots and leaves, and also an affected sulfur balance. Also shows an increased sensitivity to cadmium stress.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi46K → A: No cysteine synthase activity. 1 Publication1
Mutagenesisi74T → A: Strong reduction of cysteine synthase activity. 1 Publication1
Mutagenesisi74T → S: Reduction of cysteine synthase activity. 1 Publication1
Mutagenesisi75S → A, N or T: Strong reduction of cysteine synthase activity. 1 Publication1
Mutagenesisi77N → A: Reduction of cysteine synthase activity. 1 Publication1
Mutagenesisi77N → D: Strong reduction of cysteine synthase activity. 1 Publication1
Mutagenesisi78T → A or S: Reduction of cysteine synthase activity. 1 Publication1
Mutagenesisi147Q → A or E: Strong reduction of cysteine synthase activity. 1 Publication1
Mutagenesisi157H → Q or N: Slight reduction of cysteine synthase activity. 1 Publication1
Mutagenesisi162G → E in old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity. 1 Publication1
Mutagenesisi182T → A or S: Slight reduction of cysteine synthase activity. 1 Publication1
Mutagenesisi185T → A or S: Strong reduction of cysteine synthase activity. 1 Publication1
Mutagenesisi217K → A: Impaired interaction with SAT1. 1 Publication1
Mutagenesisi221H → A: Impaired interaction with SAT1. 1 Publication1
Mutagenesisi222K → A: Impaired interaction with SAT1. 1 Publication1
Mutagenesisi269S → A: Strong reduction of cysteine synthase activity. 1 Publication1
Mutagenesisi269S → T: Reduction of cysteine synthase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001671162 – 322Cysteine synthase 1Add BLAST321

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei46N6-(pyridoxal phosphate)lysineCombined sources2 Publications1
Modified residuei178PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP47998.
PRIDEiP47998.

PTM databases

iPTMnetiP47998.

Expressioni

Gene expression databases

GenevisibleiP47998. AT.

Interactioni

Subunit structurei

Homodimer. Interacts with SAT1. Component of the cysteine synthase complex (CSC) composed of two OAS-TL dimers and one SAT hexamer. Interacts with SULTR1;2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SULTR1;2Q9MAX35EBI-1633418,EBI-8772960

Protein-protein interaction databases

BioGridi12442. 8 interactors.
IntActiP47998. 5 interactors.
MINTiMINT-8061212.
STRINGi3702.AT4G14880.1.

Structurei

Secondary structure

1322
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 12Combined sources4
Beta strandi18 – 20Combined sources3
Helixi23 – 25Combined sources3
Beta strandi29 – 36Combined sources8
Helixi37 – 39Combined sources3
Helixi46 – 59Combined sources14
Turni65 – 67Combined sources3
Beta strandi69 – 73Combined sources5
Helixi77 – 89Combined sources13
Beta strandi92 – 98Combined sources7
Helixi103 – 111Combined sources9
Beta strandi115 – 119Combined sources5
Helixi121 – 123Combined sources3
Helixi124 – 138Combined sources15
Beta strandi142 – 144Combined sources3
Turni147 – 149Combined sources3
Helixi152 – 159Combined sources8
Helixi161 – 168Combined sources8
Turni169 – 171Combined sources3
Beta strandi175 – 179Combined sources5
Beta strandi181 – 183Combined sources3
Helixi184 – 196Combined sources13
Beta strandi201 – 207Combined sources7
Helixi208 – 210Combined sources3
Helixi212 – 214Combined sources3
Helixi237 – 239Combined sources3
Beta strandi241 – 246Combined sources6
Helixi248 – 262Combined sources15
Helixi268 – 281Combined sources14
Helixi284 – 286Combined sources3
Beta strandi290 – 295Combined sources6
Helixi299 – 302Combined sources4
Beta strandi303 – 305Combined sources3
Helixi306 – 308Combined sources3
Helixi309 – 316Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z7WX-ray2.20A1-322[»]
1Z7YX-ray2.70A1-322[»]
2ISQX-ray2.80A3-322[»]
ProteinModelPortaliP47998.
SMRiP47998.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP47998.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni144 – 287SUTR1;2 binding1 PublicationAdd BLAST144
Regioni181 – 185Pyridoxal phosphate bindingCombined sources2 Publications5
Regioni217 – 222SAT1 binding1 Publication6

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1252. Eukaryota.
COG0031. LUCA.
InParanoidiP47998.
KOiK01738.
OMAiFEDNAQS.
OrthoDBiEOG09360FNG.
PhylomeDBiP47998.

Family and domain databases

InterProiIPR005856. Cys_synth.
IPR005859. CysK.
IPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01139. cysK. 1 hit.
TIGR01136. cysKM. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47998-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASRIAKDVT ELIGNTPLVY LNNVAEGCVG RVAAKLEMME PCSSVKDRIG
60 70 80 90 100
FSMISDAEKK GLIKPGESVL IEPTSGNTGV GLAFTAAAKG YKLIITMPAS
110 120 130 140 150
MSTERRIILL AFGVELVLTD PAKGMKGAIA KAEEILAKTP NGYMLQQFEN
160 170 180 190 200
PANPKIHYET TGPEIWKGTG GKIDGFVSGI GTGGTITGAG KYLKEQNANV
210 220 230 240 250
KLYGVEPVES AILSGGKPGP HKIQGIGAGF IPSVLNVDLI DEVVQVSSDE
260 270 280 290 300
SIDMARQLAL KEGLLVGISS GAAAAAAIKL AQRPENAGKL FVAIFPSFGE
310 320
RYLSTVLFDA TRKEAEAMTF EA
Length:322
Mass (Da):33,805
Last modified:December 1, 2000 - v2
Checksum:i5B3E7F3D9DA5908B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20Missing in AAK76499 (PubMed:14593172).Curated1
Sequence conflicti273A → E in CAA58893 (PubMed:7729527).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80376 mRNA. Translation: CAA56593.2.
X84097 mRNA. Translation: CAA58893.1.
AJ272027 Genomic DNA. Translation: CAB72932.1.
Z97337 Genomic DNA. Translation: CAB10267.1.
AL161540 Genomic DNA. Translation: CAB78530.1.
CP002687 Genomic DNA. Translation: AEE83512.1.
CP002687 Genomic DNA. Translation: AEE83513.1.
CP002687 Genomic DNA. Translation: AEE83514.1.
CP002687 Genomic DNA. Translation: AEE83515.1.
AY045825 mRNA. Translation: AAK76499.1.
BT025878 mRNA. Translation: ABF85780.1.
PIRiA71412.
S48694.
RefSeqiNP_001190732.1. NM_001203803.1.
NP_001190733.1. NM_001203804.1.
NP_193224.1. NM_117574.4.
NP_849386.1. NM_179055.3.
UniGeneiAt.30.
At.34389.

Genome annotation databases

EnsemblPlantsiAT4G14880.1; AT4G14880.1; AT4G14880.
AT4G14880.2; AT4G14880.2; AT4G14880.
AT4G14880.3; AT4G14880.3; AT4G14880.
AT4G14880.4; AT4G14880.4; AT4G14880.
GeneIDi827145.
GrameneiAT4G14880.1; AT4G14880.1; AT4G14880.
AT4G14880.2; AT4G14880.2; AT4G14880.
AT4G14880.3; AT4G14880.3; AT4G14880.
AT4G14880.4; AT4G14880.4; AT4G14880.
KEGGiath:AT4G14880.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80376 mRNA. Translation: CAA56593.2.
X84097 mRNA. Translation: CAA58893.1.
AJ272027 Genomic DNA. Translation: CAB72932.1.
Z97337 Genomic DNA. Translation: CAB10267.1.
AL161540 Genomic DNA. Translation: CAB78530.1.
CP002687 Genomic DNA. Translation: AEE83512.1.
CP002687 Genomic DNA. Translation: AEE83513.1.
CP002687 Genomic DNA. Translation: AEE83514.1.
CP002687 Genomic DNA. Translation: AEE83515.1.
AY045825 mRNA. Translation: AAK76499.1.
BT025878 mRNA. Translation: ABF85780.1.
PIRiA71412.
S48694.
RefSeqiNP_001190732.1. NM_001203803.1.
NP_001190733.1. NM_001203804.1.
NP_193224.1. NM_117574.4.
NP_849386.1. NM_179055.3.
UniGeneiAt.30.
At.34389.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z7WX-ray2.20A1-322[»]
1Z7YX-ray2.70A1-322[»]
2ISQX-ray2.80A3-322[»]
ProteinModelPortaliP47998.
SMRiP47998.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi12442. 8 interactors.
IntActiP47998. 5 interactors.
MINTiMINT-8061212.
STRINGi3702.AT4G14880.1.

PTM databases

iPTMnetiP47998.

Proteomic databases

PaxDbiP47998.
PRIDEiP47998.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G14880.1; AT4G14880.1; AT4G14880.
AT4G14880.2; AT4G14880.2; AT4G14880.
AT4G14880.3; AT4G14880.3; AT4G14880.
AT4G14880.4; AT4G14880.4; AT4G14880.
GeneIDi827145.
GrameneiAT4G14880.1; AT4G14880.1; AT4G14880.
AT4G14880.2; AT4G14880.2; AT4G14880.
AT4G14880.3; AT4G14880.3; AT4G14880.
AT4G14880.4; AT4G14880.4; AT4G14880.
KEGGiath:AT4G14880.

Organism-specific databases

TAIRiAT4G14880.

Phylogenomic databases

eggNOGiKOG1252. Eukaryota.
COG0031. LUCA.
InParanoidiP47998.
KOiK01738.
OMAiFEDNAQS.
OrthoDBiEOG09360FNG.
PhylomeDBiP47998.

Enzyme and pathway databases

UniPathwayiUPA00136; UER00200.
BioCyciMetaCyc:AT4G14880-MONOMER.
BRENDAi2.5.1.47. 399.
SABIO-RKP47998.

Miscellaneous databases

EvolutionaryTraceiP47998.
PROiP47998.

Gene expression databases

GenevisibleiP47998. AT.

Family and domain databases

InterProiIPR005856. Cys_synth.
IPR005859. CysK.
IPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01139. cysK. 1 hit.
TIGR01136. cysKM. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYSK1_ARATH
AccessioniPrimary (citable) accession number: P47998
Secondary accession number(s): O23343
, Q1EC56, Q42570, Q94AS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 1, 2000
Last modified: November 30, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.