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Protein

Inosine-5'-monophosphate dehydrogenase 1

Gene

IMPDH

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

K+UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

Pathwayi: XMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes XMP from IMP.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Inosine-5'-monophosphate dehydrogenase, Inosine-5'-monophosphate dehydrogenase 2 (At1g16350), Inosine-5'-monophosphate dehydrogenase (AXX17_At1g17130), Inosine-5'-monophosphate dehydrogenase 1 (IMPDH), Inosine-5'-monophosphate dehydrogenase (AXX17_At1g74260)
This subpathway is part of the pathway XMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes XMP from IMP, the pathway XMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi317Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi319Potassium; via carbonyl oxygenUniRule annotation1
Binding sitei320IMPUniRule annotation1
Active sitei322Thioimidate intermediateUniRule annotation1
Metal bindingi322Potassium; via carbonyl oxygenUniRule annotation1
Active sitei418Proton acceptorUniRule annotation1
Binding sitei430IMPUniRule annotation1
Metal bindingi489Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
Metal bindingi490Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
Metal bindingi491Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi265 – 267NADUniRule annotation3
Nucleotide bindingi315 – 317NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGMP biosynthesis, Purine biosynthesis
LigandMetal-binding, NAD, Potassium

Enzyme and pathway databases

BioCyciARA:AT1G79470-MONOMER
ReactomeiR-ATH-6798695 Neutrophil degranulation
R-ATH-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00601; UER00295

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenase 1UniRule annotation (EC:1.1.1.205UniRule annotation)
Short name:
IMP dehydrogenase 1UniRule annotation
Short name:
IMPD 1UniRule annotation
Short name:
IMPDH 1UniRule annotation
Gene namesi
Name:IMPDHUniRule annotation
Ordered Locus Names:At1g79470
ORF Names:T8K14.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G79470
TAIRilocus:2206365 AT1G79470

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000936862 – 503Inosine-5'-monophosphate dehydrogenase 1Add BLAST502

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP47996
PRIDEiP47996

Expressioni

Gene expression databases

ExpressionAtlasiP47996 baseline and differential
GenevisibleiP47996 AT

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

BioGridi29504, 2 interactors
IntActiP47996, 2 interactors
STRINGi3702.AT1G79470.1

Structurei

3D structure databases

ProteinModelPortaliP47996
SMRiP47996
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini167 – 225CBSUniRule annotationAdd BLAST59

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni355 – 357IMP bindingUniRule annotation3
Regioni378 – 379IMP bindingUniRule annotation2
Regioni402 – 406IMP bindingUniRule annotation5

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.UniRule annotation

Keywords - Domaini

CBS domain

Phylogenomic databases

eggNOGiKOG2550 Eukaryota
COG0516 LUCA
HOGENOMiHOG000165752
KOiK00088
OMAiGIGIVHK
OrthoDBiEOG093607LZ
PhylomeDBiP47996

Family and domain databases

CDDicd00381 IMPDH, 1 hit
Gene3Di3.20.20.70, 2 hits
HAMAPiMF_01964 IMPDH, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR005990 IMP_DH
IPR015875 IMP_DH/GMP_Rdtase_CS
IPR001093 IMP_DH_GMPRt
PfamiView protein in Pfam
PF00478 IMPDH, 1 hit
PIRSFiPIRSF000130 IMPDH, 1 hit
TIGRFAMsiTIGR01302 IMP_dehydrog, 1 hit
PROSITEiView protein in PROSITE
PS00487 IMP_DH_GMP_RED, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47996-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTLEDGFPA DKLFAQGYSY TYDDVIFLPH FIDFSTDAVS LSTRLSRRVP
60 70 80 90 100
LSIPCVSSPM DTVSESHMAA AMASLGGIGI VHYNCGIAAQ ASIIRQAKSL
110 120 130 140 150
KHPIASDAGV KFPEYEITSL DAFGPSSFVF VEQTGTMTTP KLLGYVTKSQ
160 170 180 190 200
WKRMNYEQRE MKIYDYMKSC DSSDYCVPWE IDFEKLEFVL EDKQKGFVVL
210 220 230 240 250
ERDGETVNVV TKDDIQRVKG YPKSGPGTVG PDGEWMVGAA IGTRESDKER
260 270 280 290 300
LEHLVNVGVN AVVLDSSQGN SIYQLEMIKY VKKTYPELDV IGGNVVTMYQ
310 320 330 340 350
AQNLIQAGVD GLRVGMGSGS ICTTQEVCAV GRGQATAVYK VCSIAAQSGI
360 370 380 390 400
PVIADGGISN SGHIVKALVL GASTVMMGSF LAGSTEAPGG YEYTNGKRIK
410 420 430 440 450
KYRGMGSLEA MTKGSDQRYL GDQTKLKIAQ GVVGAVADKG SVLKLIPYTM
460 470 480 490 500
HAVKQGFQDL GASSLQSAHG LLRSNILRLE ARTGAAQVEG GVHGLVSYEK

KSF
Length:503
Mass (Da):54,194
Last modified:February 1, 1996 - v1
Checksum:iADDDAF9C3A697A9A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34684 Genomic DNA Translation: AAB41940.1
AC007202 Genomic DNA Translation: AAD30229.1
CP002684 Genomic DNA Translation: AEE36247.1
AF462859 mRNA Translation: AAL58945.1
BT000820 mRNA Translation: AAN33195.1
PIRiJC4999
RefSeqiNP_178065.1, NM_106595.4
UniGeneiAt.28603

Genome annotation databases

EnsemblPlantsiAT1G79470.1; AT1G79470.1; AT1G79470
GeneIDi844285
GrameneiAT1G79470.1; AT1G79470.1; AT1G79470
KEGGiath:AT1G79470

Similar proteinsi

Entry informationi

Entry nameiIMDH1_ARATH
AccessioniPrimary (citable) accession number: P47996
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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