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P47996

- IMDH1_ARATH

UniProt

P47996 - IMDH1_ARATH

Protein

Inosine-5'-monophosphate dehydrogenase 1

Gene

IMPDH

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

    Catalytic activityi

    Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

    Cofactori

    Potassium.UniRule annotation

    Enzyme regulationi

    Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi317 – 3171Potassium; via carbonyl oxygenUniRule annotation
    Metal bindingi319 – 3191Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei320 – 3201IMPUniRule annotation
    Active sitei322 – 3221Thioimidate intermediateUniRule annotation
    Metal bindingi322 – 3221Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei430 – 4301IMPUniRule annotation
    Metal bindingi489 – 4891Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi490 – 4901Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi491 – 4911Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi265 – 2673NADUniRule annotation
    Nucleotide bindingi315 – 3173NADUniRule annotation

    GO - Molecular functioni

    1. IMP dehydrogenase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-HAMAP
    3. nucleotide binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. GMP biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    GMP biosynthesis, Purine biosynthesis

    Keywords - Ligandi

    Metal-binding, NAD, Potassium

    Enzyme and pathway databases

    BioCyciARA:AT1G79470-MONOMER.
    UniPathwayiUPA00601; UER00295.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inosine-5'-monophosphate dehydrogenase 1UniRule annotation (EC:1.1.1.205UniRule annotation)
    Short name:
    IMP dehydrogenase 1UniRule annotation
    Short name:
    IMPD 1UniRule annotation
    Short name:
    IMPDH 1UniRule annotation
    Gene namesi
    Name:IMPDHUniRule annotation
    Ordered Locus Names:At1g79470
    ORF Names:T8K14.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G79470.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytosol Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedUniRule annotation
    Chaini2 – 503502Inosine-5'-monophosphate dehydrogenase 1PRO_0000093686Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP47996.
    PRIDEiP47996.

    Expressioni

    Gene expression databases

    ArrayExpressiP47996.
    GenevestigatoriP47996.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    BioGridi29504. 2 interactions.
    IntActiP47996. 2 interactions.
    STRINGi3702.AT1G79470.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP47996.
    SMRiP47996. Positions 5-503.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini167 – 22559CBSUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni355 – 3573IMP bindingUniRule annotation
    Regioni378 – 3792IMP bindingUniRule annotation
    Regioni402 – 4065IMP bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the IMPDH/GMPR family.UniRule annotation
    Contains 1 CBS domain.UniRule annotation

    Keywords - Domaini

    CBS domain

    Phylogenomic databases

    eggNOGiCOG0516.
    HOGENOMiHOG000165752.
    InParanoidiP47996.
    KOiK00088.
    OMAiFPEYEIT.
    PhylomeDBiP47996.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01964. IMPDH.
    InterProiIPR013785. Aldolase_TIM.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view]
    PANTHERiPTHR11911:SF6. PTHR11911:SF6. 1 hit.
    PfamiPF00478. IMPDH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000130. IMPDH. 1 hit.
    TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
    PROSITEiPS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P47996-1 [UniParc]FASTAAdd to Basket

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    MSTLEDGFPA DKLFAQGYSY TYDDVIFLPH FIDFSTDAVS LSTRLSRRVP    50
    LSIPCVSSPM DTVSESHMAA AMASLGGIGI VHYNCGIAAQ ASIIRQAKSL 100
    KHPIASDAGV KFPEYEITSL DAFGPSSFVF VEQTGTMTTP KLLGYVTKSQ 150
    WKRMNYEQRE MKIYDYMKSC DSSDYCVPWE IDFEKLEFVL EDKQKGFVVL 200
    ERDGETVNVV TKDDIQRVKG YPKSGPGTVG PDGEWMVGAA IGTRESDKER 250
    LEHLVNVGVN AVVLDSSQGN SIYQLEMIKY VKKTYPELDV IGGNVVTMYQ 300
    AQNLIQAGVD GLRVGMGSGS ICTTQEVCAV GRGQATAVYK VCSIAAQSGI 350
    PVIADGGISN SGHIVKALVL GASTVMMGSF LAGSTEAPGG YEYTNGKRIK 400
    KYRGMGSLEA MTKGSDQRYL GDQTKLKIAQ GVVGAVADKG SVLKLIPYTM 450
    HAVKQGFQDL GASSLQSAHG LLRSNILRLE ARTGAAQVEG GVHGLVSYEK 500
    KSF 503
    Length:503
    Mass (Da):54,194
    Last modified:February 1, 1996 - v1
    Checksum:iADDDAF9C3A697A9A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L34684 Genomic DNA. Translation: AAB41940.1.
    AC007202 Genomic DNA. Translation: AAD30229.1.
    CP002684 Genomic DNA. Translation: AEE36247.1.
    AF462859 mRNA. Translation: AAL58945.1.
    BT000820 mRNA. Translation: AAN33195.1.
    PIRiJC4999.
    RefSeqiNP_178065.1. NM_106595.3.
    UniGeneiAt.28603.

    Genome annotation databases

    EnsemblPlantsiAT1G79470.1; AT1G79470.1; AT1G79470.
    GeneIDi844285.
    KEGGiath:AT1G79470.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L34684 Genomic DNA. Translation: AAB41940.1 .
    AC007202 Genomic DNA. Translation: AAD30229.1 .
    CP002684 Genomic DNA. Translation: AEE36247.1 .
    AF462859 mRNA. Translation: AAL58945.1 .
    BT000820 mRNA. Translation: AAN33195.1 .
    PIRi JC4999.
    RefSeqi NP_178065.1. NM_106595.3.
    UniGenei At.28603.

    3D structure databases

    ProteinModelPortali P47996.
    SMRi P47996. Positions 5-503.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 29504. 2 interactions.
    IntActi P47996. 2 interactions.
    STRINGi 3702.AT1G79470.1-P.

    Proteomic databases

    PaxDbi P47996.
    PRIDEi P47996.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G79470.1 ; AT1G79470.1 ; AT1G79470 .
    GeneIDi 844285.
    KEGGi ath:AT1G79470.

    Organism-specific databases

    TAIRi AT1G79470.

    Phylogenomic databases

    eggNOGi COG0516.
    HOGENOMi HOG000165752.
    InParanoidi P47996.
    KOi K00088.
    OMAi FPEYEIT.
    PhylomeDBi P47996.

    Enzyme and pathway databases

    UniPathwayi UPA00601 ; UER00295 .
    BioCyci ARA:AT1G79470-MONOMER.

    Gene expression databases

    ArrayExpressi P47996.
    Genevestigatori P47996.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01964. IMPDH.
    InterProi IPR013785. Aldolase_TIM.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view ]
    PANTHERi PTHR11911:SF6. PTHR11911:SF6. 1 hit.
    Pfami PF00478. IMPDH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000130. IMPDH. 1 hit.
    TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
    PROSITEi PS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the gene encoding IMP dehydrogenase from Arabidopsis thaliana."
      Collart F.R., Osipiuk J., Trent J., Olsen G.J., Huberman E.
      Gene 174:217-220(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiIMDH1_ARATH
    AccessioniPrimary (citable) accession number: P47996
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3