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P47990

- XDH_CHICK

UniProt

P47990 - XDH_CHICK

Protein

Xanthine dehydrogenase/oxidase

Gene

XDH

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species By similarity.By similarity

    Catalytic activityi

    Xanthine + NAD+ + H2O = urate + NADH.
    Hypoxanthine + NAD+ + H2O = xanthine + NADH.
    Xanthine + H2O + O2 = urate + H2O2.

    Cofactori

    Binds 2 2Fe-2S clusters.By similarity
    FAD.By similarity
    Binds 1 molybdenum-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471Iron-sulfur 1By similarity
    Metal bindingi52 – 521Iron-sulfur 1By similarity
    Metal bindingi55 – 551Iron-sulfur 1By similarity
    Metal bindingi77 – 771Iron-sulfur 1By similarity
    Metal bindingi117 – 1171Iron-sulfur 2By similarity
    Metal bindingi120 – 1201Iron-sulfur 2By similarity
    Metal bindingi152 – 1521Iron-sulfur 2By similarity
    Metal bindingi154 – 1541Iron-sulfur 2By similarity
    Binding sitei363 – 3631FADBy similarity
    Binding sitei386 – 3861FADBy similarity
    Binding sitei430 – 4301FAD; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei448 – 4481FADBy similarity
    Metal bindingi796 – 7961MolybdenumBy similarity
    Metal bindingi827 – 8271Molybdenum; via carbonyl oxygenBy similarity
    Binding sitei831 – 8311SubstrateBy similarity
    Binding sitei909 – 9091SubstrateBy similarity
    Metal bindingi941 – 9411Molybdenum; via amide nitrogenBy similarity
    Binding sitei943 – 9431SubstrateBy similarity
    Binding sitei1039 – 10391Substrate; via amide nitrogenBy similarity
    Metal bindingi1108 – 11081Molybdenum; via amide nitrogenBy similarity
    Active sitei1290 – 12901Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi283 – 2908FADBy similarity
    Nucleotide bindingi373 – 3775FADBy similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB
    2. electron carrier activity Source: InterPro
    3. flavin adenine dinucleotide binding Source: UniProtKB
    4. iron ion binding Source: InterPro
    5. molybdenum ion binding Source: InterPro
    6. molybdopterin cofactor binding Source: UniProtKB
    7. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro
    8. xanthine dehydrogenase activity Source: UniProtKB
    9. xanthine oxidase activity Source: UniProtKB

    GO - Biological processi

    1. nucleobase-containing small molecule metabolic process Source: Reactome
    2. purine nucleobase metabolic process Source: Reactome
    3. small molecule metabolic process Source: Reactome
    4. urate biosynthetic process Source: Reactome
    5. xanthine catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD

    Enzyme and pathway databases

    ReactomeiREACT_115590. Urate synthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xanthine dehydrogenase/oxidase
    Including the following 2 domains:
    Xanthine dehydrogenase (EC:1.17.1.4)
    Short name:
    XD
    Xanthine oxidase (EC:1.17.3.2)
    Short name:
    XO
    Alternative name(s):
    Xanthine oxidoreductase
    Short name:
    XOR
    Gene namesi
    Name:XDH
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    Peroxisome By similarity. Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. peroxisome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13581358Xanthine dehydrogenase/oxidasePRO_0000166083Add
    BLAST

    Proteomic databases

    PaxDbiP47990.

    Expressioni

    Tissue specificityi

    Detected in liver (at protein level).1 Publication

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000014144.

    Structurei

    3D structure databases

    ProteinModelPortaliP47990.
    SMRiP47990. Positions 600-1343.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini8 – 95882Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini255 – 440186FAD-binding PCMH-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the xanthine dehydrogenase family.Curated
    Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
    Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG4630.
    HOGENOMiHOG000191197.
    HOVERGENiHBG004182.
    InParanoidiP47990.
    KOiK00106.
    PhylomeDBiP47990.

    Family and domain databases

    Gene3Di1.10.150.120. 1 hit.
    3.10.20.30. 1 hit.
    3.30.365.10. 6 hits.
    3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    3.90.1170.50. 1 hit.
    InterProiIPR002888. 2Fe-2S-bd.
    IPR001041. 2Fe-2S_ferredoxin-type.
    IPR006058. 2Fe2S_fd_BS.
    IPR000674. Ald_Oxase/Xan_DH_a/b.
    IPR016208. Ald_Oxase/xanthine_DH.
    IPR008274. AldOxase/xan_DH_Mopterin-bd.
    IPR012675. Beta-grasp_dom.
    IPR005107. CO_DH_flav_C.
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR002346. Mopterin_DH_FAD-bd.
    IPR022407. OxRdtase_Mopterin_BS.
    IPR014309. Xanthine_DH_Mopterin-bd_su.
    IPR014307. Xanthine_DH_ssu.
    [Graphical view]
    PfamiPF01315. Ald_Xan_dh_C. 1 hit.
    PF02738. Ald_Xan_dh_C2. 1 hit.
    PF03450. CO_deh_flav_C. 1 hit.
    PF00941. FAD_binding_5. 1 hit.
    PF00111. Fer2. 1 hit.
    PF01799. Fer2_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000127. Xanthine_DH. 1 hit.
    SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
    SM01092. CO_deh_flav_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF47741. SSF47741. 2 hits.
    SSF54292. SSF54292. 1 hit.
    SSF54665. SSF54665. 1 hit.
    SSF55447. SSF55447. 1 hit.
    SSF56003. SSF56003. 1 hit.
    SSF56176. SSF56176. 1 hit.
    TIGRFAMsiTIGR02963. xanthine_xdhA. 1 hit.
    TIGR02965. xanthine_xdhB. 1 hit.
    PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
    PS51085. 2FE2S_FER_2. 1 hit.
    PS51387. FAD_PCMH. 1 hit.
    PS00559. MOLYBDOPTERIN_EUK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47990-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPPETGDEL VFFVNGKKVV EKDVDPETTL LTYLRRKLGL CGTKLGCGEG     50
    GCGACTVMIS KYDPFQKKIL HHTANACLFP ICALHHVAVT TVEGIGNTKS 100
    RLHPAQERIA KSHGSQCGFC TPGIVMSMYT LLRNKPKPKM EDIEDAFQGN 150
    LCRCTGYRPI LEGYRTFAVD SNCCGKAANG TGCCHSKGEN SMNGGCCGGK 200
    ANGPGCCMNE KENVTMMSSS LFDSSEFQPL DPTQEPIFPP ELMTQRNKEQ 250
    KQVCFKGERV MWIQPTTLQE LVALKSQYPN AKLVVGNTEV GIEMRLKNML 300
    YPVILAPAWI PEMNAVQQTE TGITFGAACT LSSVEEVLRK AVAELPSYKT 350
    EIFQAALEQL RWFAGPQIRN VAALGGNIMT ASPISDLNPV LMASGSKLTL 400
    ISMEGKRTVM MDEKFFTGYR KTIVKPEEVL LSVEIPYSKE GEYFSAFKQA 450
    YRREDDIAIV TCGMRVLFQH GTSRVQEVKL SYGGMAPTTI LALKTCRELA 500
    GRDWNEKLLQ DACRLLAGEM DLSPSAPGGM VEFRRTLTLS FFFKFYLTVL 550
    QKLSKDQNGP NNLCEPVPPN YISATELFHK DPIASTQLFQ EVPRGQLVED 600
    TVGRPLVHLS AAKQACGEAV YCDDIPHYEN ELYLTLVTST QAHAKILSID 650
    ASEAQSVPGF VCFVSAKDVP GSNITGIAND ETVFAEDVVT CVGHIIGAVI 700
    ADTQEHSRRA AKAVKIKYEE LKPIVTIQEA IEQQSFIKPI KRIKKGDVNK 750
    GFEESDHILE GEMHIGGQEH FYLETHCTLA VPKGEDGEME LFVSTQNLMK 800
    TQEFTASALG VPSNRIVVRV KRMGGGFGGK ETRNTILTTV VAVAAFKTGR 850
    PVRCMLDRDE DMLISGGRHP FLGRYKVGFM KNGKIKSLEV SYYSNGGNSA 900
    DLSHGVMDRA LLHLDNSYNI PNVSIMGFIC KTNLSSNTAF RGFGGPQGMM 950
    IAECWMSDLA RKCGLPPEEV RKINLYHEGD LTHFNQKLEG FTLRRCWDEC 1000
    LSSSNYHARK KLIEEFNKQN RWKKRGMCII PTKFGISFTV PFLNQAGALV 1050
    HVYTDGSVLL THGGTEMGQG LHTKMIQVAS RSLGIPTSKI YISETSTNTV 1100
    PNTSPTAASV SADINGMAVH NACQTILKRL EPIKQSNLKG SWEDWIKTAY 1150
    ENCISLSATG FYRIPDVGYN FETNKGKPFH YFSYGVACSE VEIDCLTGDH 1200
    KNIRTDIVMD VGTSLNPAID IGQIEGAFVQ GIGLFTMEEL RYSPEGNLYT 1250
    RGPGMYKIPA FGDIPTEFYV SLLRDCPNSK AIYSSKAVGE PPLFLSASVF 1300
    YAIKDAIYSA REDSGVTEPF RLDSPATPER IRNACVDTFT KMCPSAEPGT 1350
    FKPWSVRA 1358
    Length:1,358
    Mass (Da):149,614
    Last modified:February 1, 1996 - v1
    Checksum:i53B049B38704995F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13221 mRNA. Translation: BAA02502.1.
    PIRiA55711. XOCHDH.
    RefSeqiNP_990458.1. NM_205127.1.
    UniGeneiGga.2494.

    Genome annotation databases

    GeneIDi396025.
    KEGGigga:396025.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13221 mRNA. Translation: BAA02502.1 .
    PIRi A55711. XOCHDH.
    RefSeqi NP_990458.1. NM_205127.1.
    UniGenei Gga.2494.

    3D structure databases

    ProteinModelPortali P47990.
    SMRi P47990. Positions 600-1343.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000014144.

    Proteomic databases

    PaxDbi P47990.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 396025.
    KEGGi gga:396025.

    Organism-specific databases

    CTDi 7498.

    Phylogenomic databases

    eggNOGi COG4630.
    HOGENOMi HOG000191197.
    HOVERGENi HBG004182.
    InParanoidi P47990.
    KOi K00106.
    PhylomeDBi P47990.

    Enzyme and pathway databases

    Reactomei REACT_115590. Urate synthesis.

    Miscellaneous databases

    NextBioi 20816087.
    PROi P47990.

    Family and domain databases

    Gene3Di 1.10.150.120. 1 hit.
    3.10.20.30. 1 hit.
    3.30.365.10. 6 hits.
    3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    3.90.1170.50. 1 hit.
    InterProi IPR002888. 2Fe-2S-bd.
    IPR001041. 2Fe-2S_ferredoxin-type.
    IPR006058. 2Fe2S_fd_BS.
    IPR000674. Ald_Oxase/Xan_DH_a/b.
    IPR016208. Ald_Oxase/xanthine_DH.
    IPR008274. AldOxase/xan_DH_Mopterin-bd.
    IPR012675. Beta-grasp_dom.
    IPR005107. CO_DH_flav_C.
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR002346. Mopterin_DH_FAD-bd.
    IPR022407. OxRdtase_Mopterin_BS.
    IPR014309. Xanthine_DH_Mopterin-bd_su.
    IPR014307. Xanthine_DH_ssu.
    [Graphical view ]
    Pfami PF01315. Ald_Xan_dh_C. 1 hit.
    PF02738. Ald_Xan_dh_C2. 1 hit.
    PF03450. CO_deh_flav_C. 1 hit.
    PF00941. FAD_binding_5. 1 hit.
    PF00111. Fer2. 1 hit.
    PF01799. Fer2_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000127. Xanthine_DH. 1 hit.
    SMARTi SM01008. Ald_Xan_dh_C. 1 hit.
    SM01092. CO_deh_flav_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47741. SSF47741. 2 hits.
    SSF54292. SSF54292. 1 hit.
    SSF54665. SSF54665. 1 hit.
    SSF55447. SSF55447. 1 hit.
    SSF56003. SSF56003. 1 hit.
    SSF56176. SSF56176. 1 hit.
    TIGRFAMsi TIGR02963. xanthine_xdhA. 1 hit.
    TIGR02965. xanthine_xdhB. 1 hit.
    PROSITEi PS00197. 2FE2S_FER_1. 1 hit.
    PS51085. 2FE2S_FER_2. 1 hit.
    PS51387. FAD_PCMH. 1 hit.
    PS00559. MOLYBDOPTERIN_EUK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The structure of chicken liver xanthine dehydrogenase. cDNA cloning and the domain structure."
      Satoh A., Amaya Y., Noda K., Nishino T.
      J. Biol. Chem. 270:2818-2826(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CATALYTIC ACTIVITY.
      Tissue: Liver.
    2. "Subcellular localization of chicken liver xanthine dehydrogenase. A possible source of cytoplasmic reducing equivalents."
      Coolbear K.P., Herzberg G.R., Brosnan J.T.
      Biochem. J. 202:555-558(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, CATALYTIC ACTIVITY.

    Entry informationi

    Entry nameiXDH_CHICK
    AccessioniPrimary (citable) accession number: P47990
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3