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Protein

Xanthine dehydrogenase/oxidase

Gene

XDH

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species.By similarity

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.2 Publications
Hypoxanthine + NAD+ + H2O = xanthine + NADH.2 Publications
Xanthine + H2O + O2 = urate + H2O2.By similarity

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 2 [2Fe-2S] clusters.By similarity
  • FADBy similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Iron-sulfur 1By similarity1
Metal bindingi52Iron-sulfur 1By similarity1
Metal bindingi55Iron-sulfur 1By similarity1
Metal bindingi77Iron-sulfur 1By similarity1
Metal bindingi117Iron-sulfur 2By similarity1
Metal bindingi120Iron-sulfur 2By similarity1
Metal bindingi152Iron-sulfur 2By similarity1
Metal bindingi154Iron-sulfur 2By similarity1
Binding sitei363FADBy similarity1
Binding sitei386FADBy similarity1
Binding sitei430FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei448FADBy similarity1
Metal bindingi796MolybdenumBy similarity1
Metal bindingi827Molybdenum; via carbonyl oxygenBy similarity1
Binding sitei831SubstrateBy similarity1
Binding sitei909SubstrateBy similarity1
Metal bindingi941Molybdenum; via amide nitrogenBy similarity1
Binding sitei943SubstrateBy similarity1
Binding sitei1039Substrate; via amide nitrogenBy similarity1
Metal bindingi1108Molybdenum; via amide nitrogenBy similarity1
Active sitei1290Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi283 – 290FADBy similarity8
Nucleotide bindingi373 – 377FADBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD

Enzyme and pathway databases

ReactomeiR-GGA-421178. Urate synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Xanthine dehydrogenase/oxidase
Including the following 2 domains:
Xanthine dehydrogenase (EC:1.17.1.42 Publications)
Short name:
XD
Xanthine oxidase (EC:1.17.3.2By similarity)
Short name:
XO
Alternative name(s):
Xanthine oxidoreductase
Short name:
XOR
Gene namesi
Name:XDH
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660831 – 1358Xanthine dehydrogenase/oxidaseAdd BLAST1358

Proteomic databases

PaxDbiP47990.

Expressioni

Tissue specificityi

Detected in liver (at protein level).1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000014144.

Structurei

3D structure databases

ProteinModelPortaliP47990.
SMRiP47990.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 952Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST88
Domaini255 – 440FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST186

Sequence similaritiesi

Belongs to the xanthine dehydrogenase family.Curated
Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0430. Eukaryota.
COG4630. LUCA.
COG4631. LUCA.
HOGENOMiHOG000191197.
HOVERGENiHBG004182.
InParanoidiP47990.
KOiK00106.
PhylomeDBiP47990.

Family and domain databases

Gene3Di1.10.150.120. 1 hit.
3.10.20.30. 1 hit.
3.30.365.10. 6 hits.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.1170.50. 1 hit.
InterProiIPR002888. 2Fe-2S-bd.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR000674. Ald_Oxase/Xan_DH_a/b.
IPR016208. Ald_Oxase/xanthine_DH.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
IPR012675. Beta-grasp_dom.
IPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
IPR022407. OxRdtase_Mopterin_BS.
IPR014309. Xanthine_DH_Mopterin-bd_su.
IPR014307. Xanthine_DH_ssu.
[Graphical view]
PfamiPF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
PF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
PF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000127. Xanthine_DH. 1 hit.
SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
SM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF47741. SSF47741. 2 hits.
SSF54292. SSF54292. 1 hit.
SSF54665. SSF54665. 1 hit.
SSF55447. SSF55447. 1 hit.
SSF56003. SSF56003. 1 hit.
SSF56176. SSF56176. 1 hit.
TIGRFAMsiTIGR02963. xanthine_xdhA. 1 hit.
TIGR02965. xanthine_xdhB. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51387. FAD_PCMH. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47990-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPETGDEL VFFVNGKKVV EKDVDPETTL LTYLRRKLGL CGTKLGCGEG
60 70 80 90 100
GCGACTVMIS KYDPFQKKIL HHTANACLFP ICALHHVAVT TVEGIGNTKS
110 120 130 140 150
RLHPAQERIA KSHGSQCGFC TPGIVMSMYT LLRNKPKPKM EDIEDAFQGN
160 170 180 190 200
LCRCTGYRPI LEGYRTFAVD SNCCGKAANG TGCCHSKGEN SMNGGCCGGK
210 220 230 240 250
ANGPGCCMNE KENVTMMSSS LFDSSEFQPL DPTQEPIFPP ELMTQRNKEQ
260 270 280 290 300
KQVCFKGERV MWIQPTTLQE LVALKSQYPN AKLVVGNTEV GIEMRLKNML
310 320 330 340 350
YPVILAPAWI PEMNAVQQTE TGITFGAACT LSSVEEVLRK AVAELPSYKT
360 370 380 390 400
EIFQAALEQL RWFAGPQIRN VAALGGNIMT ASPISDLNPV LMASGSKLTL
410 420 430 440 450
ISMEGKRTVM MDEKFFTGYR KTIVKPEEVL LSVEIPYSKE GEYFSAFKQA
460 470 480 490 500
YRREDDIAIV TCGMRVLFQH GTSRVQEVKL SYGGMAPTTI LALKTCRELA
510 520 530 540 550
GRDWNEKLLQ DACRLLAGEM DLSPSAPGGM VEFRRTLTLS FFFKFYLTVL
560 570 580 590 600
QKLSKDQNGP NNLCEPVPPN YISATELFHK DPIASTQLFQ EVPRGQLVED
610 620 630 640 650
TVGRPLVHLS AAKQACGEAV YCDDIPHYEN ELYLTLVTST QAHAKILSID
660 670 680 690 700
ASEAQSVPGF VCFVSAKDVP GSNITGIAND ETVFAEDVVT CVGHIIGAVI
710 720 730 740 750
ADTQEHSRRA AKAVKIKYEE LKPIVTIQEA IEQQSFIKPI KRIKKGDVNK
760 770 780 790 800
GFEESDHILE GEMHIGGQEH FYLETHCTLA VPKGEDGEME LFVSTQNLMK
810 820 830 840 850
TQEFTASALG VPSNRIVVRV KRMGGGFGGK ETRNTILTTV VAVAAFKTGR
860 870 880 890 900
PVRCMLDRDE DMLISGGRHP FLGRYKVGFM KNGKIKSLEV SYYSNGGNSA
910 920 930 940 950
DLSHGVMDRA LLHLDNSYNI PNVSIMGFIC KTNLSSNTAF RGFGGPQGMM
960 970 980 990 1000
IAECWMSDLA RKCGLPPEEV RKINLYHEGD LTHFNQKLEG FTLRRCWDEC
1010 1020 1030 1040 1050
LSSSNYHARK KLIEEFNKQN RWKKRGMCII PTKFGISFTV PFLNQAGALV
1060 1070 1080 1090 1100
HVYTDGSVLL THGGTEMGQG LHTKMIQVAS RSLGIPTSKI YISETSTNTV
1110 1120 1130 1140 1150
PNTSPTAASV SADINGMAVH NACQTILKRL EPIKQSNLKG SWEDWIKTAY
1160 1170 1180 1190 1200
ENCISLSATG FYRIPDVGYN FETNKGKPFH YFSYGVACSE VEIDCLTGDH
1210 1220 1230 1240 1250
KNIRTDIVMD VGTSLNPAID IGQIEGAFVQ GIGLFTMEEL RYSPEGNLYT
1260 1270 1280 1290 1300
RGPGMYKIPA FGDIPTEFYV SLLRDCPNSK AIYSSKAVGE PPLFLSASVF
1310 1320 1330 1340 1350
YAIKDAIYSA REDSGVTEPF RLDSPATPER IRNACVDTFT KMCPSAEPGT

FKPWSVRA
Length:1,358
Mass (Da):149,614
Last modified:February 1, 1996 - v1
Checksum:i53B049B38704995F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13221 mRNA. Translation: BAA02502.1.
PIRiA55711. XOCHDH.
RefSeqiNP_990458.1. NM_205127.1.
UniGeneiGga.2494.

Genome annotation databases

GeneIDi396025.
KEGGigga:396025.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13221 mRNA. Translation: BAA02502.1.
PIRiA55711. XOCHDH.
RefSeqiNP_990458.1. NM_205127.1.
UniGeneiGga.2494.

3D structure databases

ProteinModelPortaliP47990.
SMRiP47990.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000014144.

Proteomic databases

PaxDbiP47990.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396025.
KEGGigga:396025.

Organism-specific databases

CTDi7498.

Phylogenomic databases

eggNOGiKOG0430. Eukaryota.
COG4630. LUCA.
COG4631. LUCA.
HOGENOMiHOG000191197.
HOVERGENiHBG004182.
InParanoidiP47990.
KOiK00106.
PhylomeDBiP47990.

Enzyme and pathway databases

ReactomeiR-GGA-421178. Urate synthesis.

Miscellaneous databases

PROiP47990.

Family and domain databases

Gene3Di1.10.150.120. 1 hit.
3.10.20.30. 1 hit.
3.30.365.10. 6 hits.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.1170.50. 1 hit.
InterProiIPR002888. 2Fe-2S-bd.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR000674. Ald_Oxase/Xan_DH_a/b.
IPR016208. Ald_Oxase/xanthine_DH.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
IPR012675. Beta-grasp_dom.
IPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
IPR022407. OxRdtase_Mopterin_BS.
IPR014309. Xanthine_DH_Mopterin-bd_su.
IPR014307. Xanthine_DH_ssu.
[Graphical view]
PfamiPF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
PF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
PF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000127. Xanthine_DH. 1 hit.
SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
SM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF47741. SSF47741. 2 hits.
SSF54292. SSF54292. 1 hit.
SSF54665. SSF54665. 1 hit.
SSF55447. SSF55447. 1 hit.
SSF56003. SSF56003. 1 hit.
SSF56176. SSF56176. 1 hit.
TIGRFAMsiTIGR02963. xanthine_xdhA. 1 hit.
TIGR02965. xanthine_xdhB. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51387. FAD_PCMH. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiXDH_CHICK
AccessioniPrimary (citable) accession number: P47990
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.