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P47990

- XDH_CHICK

UniProt

P47990 - XDH_CHICK

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Protein
Xanthine dehydrogenase/oxidase
Gene
XDH
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species By similarity.

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.2 Publications
Hypoxanthine + NAD+ + H2O = xanthine + NADH.2 Publications
Xanthine + H2O + O2 = urate + H2O2.2 Publications

Cofactori

Binds 2 2Fe-2S clusters By similarity.
FAD By similarity.
Binds 1 molybdenum-molybdopterin (Mo-MPT) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron-sulfur 1 By similarity
Metal bindingi52 – 521Iron-sulfur 1 By similarity
Metal bindingi55 – 551Iron-sulfur 1 By similarity
Metal bindingi77 – 771Iron-sulfur 1 By similarity
Metal bindingi117 – 1171Iron-sulfur 2 By similarity
Metal bindingi120 – 1201Iron-sulfur 2 By similarity
Metal bindingi152 – 1521Iron-sulfur 2 By similarity
Metal bindingi154 – 1541Iron-sulfur 2 By similarity
Binding sitei363 – 3631FAD By similarity
Binding sitei386 – 3861FAD By similarity
Binding sitei430 – 4301FAD; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei448 – 4481FAD By similarity
Metal bindingi796 – 7961Molybdenum By similarity
Metal bindingi827 – 8271Molybdenum; via carbonyl oxygen By similarity
Binding sitei831 – 8311Substrate By similarity
Binding sitei909 – 9091Substrate By similarity
Metal bindingi941 – 9411Molybdenum; via amide nitrogen By similarity
Binding sitei943 – 9431Substrate By similarity
Binding sitei1039 – 10391Substrate; via amide nitrogen By similarity
Metal bindingi1108 – 11081Molybdenum; via amide nitrogen By similarity
Active sitei1290 – 12901Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi283 – 2908FAD By similarity
Nucleotide bindingi373 – 3775FAD By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB
  2. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro
  3. electron carrier activity Source: InterPro
  4. flavin adenine dinucleotide binding Source: UniProtKB
  5. iron ion binding Source: InterPro
  6. molybdenum ion binding Source: InterPro
  7. molybdopterin cofactor binding Source: UniProtKB
  8. xanthine dehydrogenase activity Source: UniProtKB
  9. xanthine oxidase activity Source: UniProtKB

GO - Biological processi

  1. nucleobase-containing small molecule metabolic process Source: Reactome
  2. purine nucleobase metabolic process Source: Reactome
  3. small molecule metabolic process Source: Reactome
  4. urate biosynthetic process Source: Reactome
  5. xanthine catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD

Enzyme and pathway databases

ReactomeiREACT_115590. Urate synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Xanthine dehydrogenase/oxidase
Including the following 2 domains:
Xanthine dehydrogenase (EC:1.17.1.4)
Short name:
XD
Xanthine oxidase (EC:1.17.3.2)
Short name:
XO
Alternative name(s):
Xanthine oxidoreductase
Short name:
XOR
Gene namesi
Name:XDH
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Peroxisome By similarity. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. peroxisome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13581358Xanthine dehydrogenase/oxidase
PRO_0000166083Add
BLAST

Proteomic databases

PaxDbiP47990.

Expressioni

Tissue specificityi

Detected in liver (at protein level).1 Publication

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

STRINGi9031.ENSGALP00000014144.

Structurei

3D structure databases

ProteinModelPortaliP47990.
SMRiP47990. Positions 600-1343.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 95882Fe-2S ferredoxin-type
Add
BLAST
Domaini255 – 440186FAD-binding PCMH-type
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG4630.
HOGENOMiHOG000191197.
HOVERGENiHBG004182.
InParanoidiP47990.
KOiK00106.
PhylomeDBiP47990.

Family and domain databases

Gene3Di1.10.150.120. 1 hit.
3.10.20.30. 1 hit.
3.30.365.10. 6 hits.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.1170.50. 1 hit.
InterProiIPR002888. 2Fe-2S-bd.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR000674. Ald_Oxase/Xan_DH_a/b.
IPR016208. Ald_Oxase/xanthine_DH.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
IPR012675. Beta-grasp_dom.
IPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
IPR022407. OxRdtase_Mopterin_BS.
IPR014309. Xanthine_DH_Mopterin-bd_su.
IPR014307. Xanthine_DH_ssu.
[Graphical view]
PfamiPF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
PF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
PF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000127. Xanthine_DH. 1 hit.
SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
SM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF47741. SSF47741. 2 hits.
SSF54292. SSF54292. 1 hit.
SSF54665. SSF54665. 1 hit.
SSF55447. SSF55447. 1 hit.
SSF56003. SSF56003. 1 hit.
SSF56176. SSF56176. 1 hit.
TIGRFAMsiTIGR02963. xanthine_xdhA. 1 hit.
TIGR02965. xanthine_xdhB. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51387. FAD_PCMH. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47990-1 [UniParc]FASTAAdd to Basket

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MAPPETGDEL VFFVNGKKVV EKDVDPETTL LTYLRRKLGL CGTKLGCGEG     50
GCGACTVMIS KYDPFQKKIL HHTANACLFP ICALHHVAVT TVEGIGNTKS 100
RLHPAQERIA KSHGSQCGFC TPGIVMSMYT LLRNKPKPKM EDIEDAFQGN 150
LCRCTGYRPI LEGYRTFAVD SNCCGKAANG TGCCHSKGEN SMNGGCCGGK 200
ANGPGCCMNE KENVTMMSSS LFDSSEFQPL DPTQEPIFPP ELMTQRNKEQ 250
KQVCFKGERV MWIQPTTLQE LVALKSQYPN AKLVVGNTEV GIEMRLKNML 300
YPVILAPAWI PEMNAVQQTE TGITFGAACT LSSVEEVLRK AVAELPSYKT 350
EIFQAALEQL RWFAGPQIRN VAALGGNIMT ASPISDLNPV LMASGSKLTL 400
ISMEGKRTVM MDEKFFTGYR KTIVKPEEVL LSVEIPYSKE GEYFSAFKQA 450
YRREDDIAIV TCGMRVLFQH GTSRVQEVKL SYGGMAPTTI LALKTCRELA 500
GRDWNEKLLQ DACRLLAGEM DLSPSAPGGM VEFRRTLTLS FFFKFYLTVL 550
QKLSKDQNGP NNLCEPVPPN YISATELFHK DPIASTQLFQ EVPRGQLVED 600
TVGRPLVHLS AAKQACGEAV YCDDIPHYEN ELYLTLVTST QAHAKILSID 650
ASEAQSVPGF VCFVSAKDVP GSNITGIAND ETVFAEDVVT CVGHIIGAVI 700
ADTQEHSRRA AKAVKIKYEE LKPIVTIQEA IEQQSFIKPI KRIKKGDVNK 750
GFEESDHILE GEMHIGGQEH FYLETHCTLA VPKGEDGEME LFVSTQNLMK 800
TQEFTASALG VPSNRIVVRV KRMGGGFGGK ETRNTILTTV VAVAAFKTGR 850
PVRCMLDRDE DMLISGGRHP FLGRYKVGFM KNGKIKSLEV SYYSNGGNSA 900
DLSHGVMDRA LLHLDNSYNI PNVSIMGFIC KTNLSSNTAF RGFGGPQGMM 950
IAECWMSDLA RKCGLPPEEV RKINLYHEGD LTHFNQKLEG FTLRRCWDEC 1000
LSSSNYHARK KLIEEFNKQN RWKKRGMCII PTKFGISFTV PFLNQAGALV 1050
HVYTDGSVLL THGGTEMGQG LHTKMIQVAS RSLGIPTSKI YISETSTNTV 1100
PNTSPTAASV SADINGMAVH NACQTILKRL EPIKQSNLKG SWEDWIKTAY 1150
ENCISLSATG FYRIPDVGYN FETNKGKPFH YFSYGVACSE VEIDCLTGDH 1200
KNIRTDIVMD VGTSLNPAID IGQIEGAFVQ GIGLFTMEEL RYSPEGNLYT 1250
RGPGMYKIPA FGDIPTEFYV SLLRDCPNSK AIYSSKAVGE PPLFLSASVF 1300
YAIKDAIYSA REDSGVTEPF RLDSPATPER IRNACVDTFT KMCPSAEPGT 1350
FKPWSVRA 1358
Length:1,358
Mass (Da):149,614
Last modified:February 1, 1996 - v1
Checksum:i53B049B38704995F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13221 mRNA. Translation: BAA02502.1.
PIRiA55711. XOCHDH.
RefSeqiNP_990458.1. NM_205127.1.
UniGeneiGga.2494.

Genome annotation databases

GeneIDi396025.
KEGGigga:396025.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13221 mRNA. Translation: BAA02502.1 .
PIRi A55711. XOCHDH.
RefSeqi NP_990458.1. NM_205127.1.
UniGenei Gga.2494.

3D structure databases

ProteinModelPortali P47990.
SMRi P47990. Positions 600-1343.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000014144.

Proteomic databases

PaxDbi P47990.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 396025.
KEGGi gga:396025.

Organism-specific databases

CTDi 7498.

Phylogenomic databases

eggNOGi COG4630.
HOGENOMi HOG000191197.
HOVERGENi HBG004182.
InParanoidi P47990.
KOi K00106.
PhylomeDBi P47990.

Enzyme and pathway databases

Reactomei REACT_115590. Urate synthesis.

Miscellaneous databases

NextBioi 20816087.
PROi P47990.

Family and domain databases

Gene3Di 1.10.150.120. 1 hit.
3.10.20.30. 1 hit.
3.30.365.10. 6 hits.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.1170.50. 1 hit.
InterProi IPR002888. 2Fe-2S-bd.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR000674. Ald_Oxase/Xan_DH_a/b.
IPR016208. Ald_Oxase/xanthine_DH.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
IPR012675. Beta-grasp_dom.
IPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
IPR022407. OxRdtase_Mopterin_BS.
IPR014309. Xanthine_DH_Mopterin-bd_su.
IPR014307. Xanthine_DH_ssu.
[Graphical view ]
Pfami PF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
PF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
PF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000127. Xanthine_DH. 1 hit.
SMARTi SM01008. Ald_Xan_dh_C. 1 hit.
SM01092. CO_deh_flav_C. 1 hit.
[Graphical view ]
SUPFAMi SSF47741. SSF47741. 2 hits.
SSF54292. SSF54292. 1 hit.
SSF54665. SSF54665. 1 hit.
SSF55447. SSF55447. 1 hit.
SSF56003. SSF56003. 1 hit.
SSF56176. SSF56176. 1 hit.
TIGRFAMsi TIGR02963. xanthine_xdhA. 1 hit.
TIGR02965. xanthine_xdhB. 1 hit.
PROSITEi PS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51387. FAD_PCMH. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The structure of chicken liver xanthine dehydrogenase. cDNA cloning and the domain structure."
    Satoh A., Amaya Y., Noda K., Nishino T.
    J. Biol. Chem. 270:2818-2826(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CATALYTIC ACTIVITY.
    Tissue: Liver.
  2. "Subcellular localization of chicken liver xanthine dehydrogenase. A possible source of cytoplasmic reducing equivalents."
    Coolbear K.P., Herzberg G.R., Brosnan J.T.
    Biochem. J. 202:555-558(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiXDH_CHICK
AccessioniPrimary (citable) accession number: P47990
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 3, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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