Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P47985

- UCRI_HUMAN

UniProt

P47985 - UCRI_HUMAN

Protein

Cytochrome b-c1 complex subunit Rieske, mitochondrial

Gene

UQCRFS1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 148 (01 Oct 2014)
      Sequence version 2 (03 Apr 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
    The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex.By similarity

    Catalytic activityi

    Quinol + 2 ferricytochrome c = quinone + 2 ferrocytochrome c + 2 H+.

    Cofactori

    Binds 1 2Fe-2S cluster per subunit.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi217 – 2171Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
    Metal bindingi219 – 2191Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation
    Metal bindingi236 – 2361Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
    Metal bindingi239 – 2391Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. ubiquinol-cytochrome-c reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular metabolic process Source: Reactome
    2. respiratory electron transport chain Source: Reactome
    3. response to antibiotic Source: Ensembl
    4. response to drug Source: Ensembl
    5. response to hormone Source: Ensembl
    6. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_22393. Respiratory electron transport.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome b-c1 complex subunit Rieske, mitochondrial (EC:1.10.2.2)
    Alternative name(s):
    Complex III subunit 5
    Cytochrome b-c1 complex subunit 5
    Rieske iron-sulfur protein
    Short name:
    RISP
    Ubiquinol-cytochrome c reductase iron-sulfur subunit
    Cleaved into the following chain:
    Alternative name(s):
    Complex III subunit IX
    Ubiquinol-cytochrome c reductase 8 kDa protein
    Gene namesi
    Name:UQCRFS1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:12587. UQCRFS1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: Reactome
    2. mitochondrial respiratory chain complex III Source: Ensembl
    3. mitochondrion Source: UniProt

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA37218.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 7878Mitochondrion1 PublicationAdd
    BLAST
    Chaini1 – 7878Cytochrome b-c1 complex subunit 11By similarityPRO_0000307241Add
    BLAST
    Chaini79 – 274196Cytochrome b-c1 complex subunit Rieske, mitochondrialPRO_0000030664Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi222 ↔ 238PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiP47985.
    PRIDEiP47985.

    2D gel databases

    OGPiP47985.
    REPRODUCTION-2DPAGEIPI00026964.
    SWISS-2DPAGEP47985.
    UCD-2DPAGEP47985.

    PTM databases

    PhosphoSiteiP47985.

    Expressioni

    Gene expression databases

    BgeeiP47985.
    CleanExiHS_UQCRFS1.
    GenevestigatoriP47985.

    Organism-specific databases

    HPAiHPA041863.

    Interactioni

    Subunit structurei

    The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1).

    Protein-protein interaction databases

    BioGridi113232. 33 interactions.
    IntActiP47985. 6 interactions.
    MINTiMINT-2803370.
    STRINGi9606.ENSP00000306397.

    Structurei

    3D structure databases

    ProteinModelPortaliP47985.
    SMRiP47985. Positions 1-57, 79-274.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei103 – 14038HelicalBy similarityAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini187 – 27286RieskePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Rieske domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG001040.
    InParanoidiP47985.
    KOiK00411.
    OMAiDYRRAEV.
    OrthoDBiEOG7GBFXQ.
    PhylomeDBiP47985.
    TreeFamiTF105037.

    Family and domain databases

    Gene3Di1.20.5.270. 1 hit.
    2.102.10.10. 1 hit.
    InterProiIPR011070. Globular_prot_asu/bsu.
    IPR017941. Rieske_2Fe-2S.
    IPR014349. Rieske_Fe-S_prot.
    IPR005805. Rieske_Fe-S_prot_C.
    IPR015248. Ubiqinol_cyt_c_Rdtase_N.
    IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su.
    IPR004192. Ubiquinol_cyt_Rdtase_TM.
    [Graphical view]
    PANTHERiPTHR10134. PTHR10134. 1 hit.
    PfamiPF00355. Rieske. 1 hit.
    PF09165. Ubiq-Cytc-red_N. 1 hit.
    PF02921. UCR_TM. 1 hit.
    [Graphical view]
    PRINTSiPR00162. RIESKE.
    SUPFAMiSSF50022. SSF50022. 1 hit.
    SSF56568. SSF56568. 1 hit.
    TIGRFAMsiTIGR01416. Rieske_proteo. 1 hit.
    PROSITEiPS51296. RIESKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P47985-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLSVASRSGP FAPVLSATSR GVAGALRPLV QATVPATPEQ PVLDLKRPFL    50
    SRESLSGQAV RRPLVASVGL NVPASVCYSH TDIKVPDFSE YRRLEVLDST 100
    KSSRESSEAR KGFSYLVTGV TTVGVAYAAK NAVTQFVSSM SASADVLALA 150
    KIEIKLSDIP EGKNMAFKWR GKPLFVRHRT QKEIEQEAAV ELSQLRDPQH 200
    DLDRVKKPEW VILIGVCTHL GCVPIANAGD FGGYYCPCHG SHYDASGRIR 250
    LGPAPLNLEV PTYEFTSDDM VIVG 274
    Length:274
    Mass (Da):29,668
    Last modified:April 3, 2007 - v2
    Checksum:i8DB51634DEB039B0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti73 – 731P → H in AAH67832. (PubMed:15489334)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti6 – 61S → A.4 Publications
    Corresponds to variant rs8100724 [ dbSNP | Ensembl ].
    VAR_051863

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L32977, L32917 Genomic DNA. Translation: AAC41754.1.
    AK291134 mRNA. Translation: BAF83823.1.
    AC007786 Genomic DNA. Translation: AAD38242.1.
    BC000649 mRNA. Translation: AAH00649.1.
    BC010035 mRNA. Translation: AAH10035.1.
    BC067832 mRNA. Translation: AAH67832.1.
    CCDSiCCDS12415.1.
    RefSeqiNP_005994.2. NM_006003.2.
    UniGeneiHs.743307.

    Genome annotation databases

    EnsembliENST00000304863; ENSP00000306397; ENSG00000169021.
    GeneIDi7386.
    KEGGihsa:7386.
    UCSCiuc002nsd.2. human.

    Polymorphism databases

    DMDMi143811471.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L32977 , L32917 Genomic DNA. Translation: AAC41754.1 .
    AK291134 mRNA. Translation: BAF83823.1 .
    AC007786 Genomic DNA. Translation: AAD38242.1 .
    BC000649 mRNA. Translation: AAH00649.1 .
    BC010035 mRNA. Translation: AAH10035.1 .
    BC067832 mRNA. Translation: AAH67832.1 .
    CCDSi CCDS12415.1.
    RefSeqi NP_005994.2. NM_006003.2.
    UniGenei Hs.743307.

    3D structure databases

    ProteinModelPortali P47985.
    SMRi P47985. Positions 1-57, 79-274.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113232. 33 interactions.
    IntActi P47985. 6 interactions.
    MINTi MINT-2803370.
    STRINGi 9606.ENSP00000306397.

    PTM databases

    PhosphoSitei P47985.

    Polymorphism databases

    DMDMi 143811471.

    2D gel databases

    OGPi P47985.
    REPRODUCTION-2DPAGE IPI00026964.
    SWISS-2DPAGE P47985.
    UCD-2DPAGE P47985.

    Proteomic databases

    MaxQBi P47985.
    PRIDEi P47985.

    Protocols and materials databases

    DNASUi 7386.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000304863 ; ENSP00000306397 ; ENSG00000169021 .
    GeneIDi 7386.
    KEGGi hsa:7386.
    UCSCi uc002nsd.2. human.

    Organism-specific databases

    CTDi 7386.
    GeneCardsi GC19M029698.
    H-InvDB HIX0041192.
    HGNCi HGNC:12587. UQCRFS1.
    HPAi HPA041863.
    MIMi 191327. gene.
    neXtProti NX_P47985.
    PharmGKBi PA37218.
    GenAtlasi Search...

    Phylogenomic databases

    HOVERGENi HBG001040.
    InParanoidi P47985.
    KOi K00411.
    OMAi DYRRAEV.
    OrthoDBi EOG7GBFXQ.
    PhylomeDBi P47985.
    TreeFami TF105037.

    Enzyme and pathway databases

    Reactomei REACT_22393. Respiratory electron transport.

    Miscellaneous databases

    ChiTaRSi UQCRFS1. human.
    GeneWikii UQCRFS1.
    GenomeRNAii 7386.
    NextBioi 28920.
    PROi P47985.
    SOURCEi Search...

    Gene expression databases

    Bgeei P47985.
    CleanExi HS_UQCRFS1.
    Genevestigatori P47985.

    Family and domain databases

    Gene3Di 1.20.5.270. 1 hit.
    2.102.10.10. 1 hit.
    InterProi IPR011070. Globular_prot_asu/bsu.
    IPR017941. Rieske_2Fe-2S.
    IPR014349. Rieske_Fe-S_prot.
    IPR005805. Rieske_Fe-S_prot_C.
    IPR015248. Ubiqinol_cyt_c_Rdtase_N.
    IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su.
    IPR004192. Ubiquinol_cyt_Rdtase_TM.
    [Graphical view ]
    PANTHERi PTHR10134. PTHR10134. 1 hit.
    Pfami PF00355. Rieske. 1 hit.
    PF09165. Ubiq-Cytc-red_N. 1 hit.
    PF02921. UCR_TM. 1 hit.
    [Graphical view ]
    PRINTSi PR00162. RIESKE.
    SUPFAMi SSF50022. SSF50022. 1 hit.
    SSF56568. SSF56568. 1 hit.
    TIGRFAMsi TIGR01416. Rieske_proteo. 1 hit.
    PROSITEi PS51296. RIESKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The primary structure of human Rieske iron-sulfur protein of mitochondrial cytochrome bc1 complex deduced from cDNA analysis."
      Nishikimi M., Hosokawa Y., Toda H., Suzuki H., Ozawa T.
      Biochem. Int. 20:155-160(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ALA-6.
    2. "Structure, sequence and location of the UQCRFS1 gene for the human Rieske Fe-S protein."
      Pennacchio L., Bergmann A., Fukushima A., Salemi A., Okubo K., Lennon G.
      Gene 155:207-211(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-6.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-6.
    4. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-6.
      Tissue: Brain, Lung and Lymph.
    6. Cited for: PROTEIN SEQUENCE OF 79-90.
      Tissue: Kidney.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiUCRI_HUMAN
    AccessioniPrimary (citable) accession number: P47985
    Secondary accession number(s): A8K519, Q6NVX5, Q9UPH2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: April 3, 2007
    Last modified: October 1, 2014
    This is version 148 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    The Rieske protein is a high potential 2Fe-2S protein.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3