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Reviewed, UniProtKB/Swiss-Prot P47985 (UCRI_HUMAN)

Last modified November 25, 2008. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Cytochrome b-c1 complex subunit Rieske, mitochondrial
    EC=1.10.2.2
Alternative name(s):
    Ubiquinol-cytochrome c reductase iron-sulfur subunit
    Rieske iron-sulfur protein
      Short name=RISP
    Complex III subunit 5
Cleaved into the following chain:
    1- Recommended name:
            Cytochrome b-c1 complex subunit 11
        Alternative name(s):
            Ubiquinol-cytochrome c reductase 8 kDa protein
            Complex III subunit IX
Gene names
Name: UQCRFS1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.

The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex By similarity.

Catalytic activity

QH(2) + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H(+).

Cofactor

Binds 1 2Fe-2S cluster per subunit By similarity.

Subunit structure

The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1).

Subcellular location

Mitochondrion inner membrane.

Miscellaneous

The Rieske protein is a high potential 2Fe-2S protein.

Sequence similarities

Contains 1 Rieske domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7878Cytochrome b-c1 complex subunit 11 By similarity
PRO_0000307241
Transit peptide1 – 7878Mitochondrion
Chain79 – 274196Cytochrome b-c1 complex subunit Rieske, mitochondrial
PRO_0000030664

Regions

Domain187 – 27286Rieske

Sites

Metal binding2171Iron-sulfur (2Fe-2S) By similarity
Metal binding2191Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Metal binding2361Iron-sulfur (2Fe-2S) By similarity
Metal binding2391Iron-sulfur (2Fe-2S); via pros nitrogen By similarity

Amino acid modifications

Disulfide bond222 ↔ 238 By similarity

Experimental info

Sequence conflict61S → A in AAC41754. Ref.1 Ref.2
Sequence conflict61S → A in AAH00649, AAH10035 and AAH67832. Ref.4
Sequence conflict731P → H in AAH67832. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P47985-1 [UniParc].

Last modified April 3, 2007. Version 2.
Checksum: 8DB51634DEB039B0

FASTA27429,668
        10         20         30         40         50         60 
MLSVASRSGP FAPVLSATSR GVAGALRPLV QATVPATPEQ PVLDLKRPFL SRESLSGQAV 

        70         80         90        100        110        120 
RRPLVASVGL NVPASVCYSH TDIKVPDFSE YRRLEVLDST KSSRESSEAR KGFSYLVTGV 

       130        140        150        160        170        180 
TTVGVAYAAK NAVTQFVSSM SASADVLALA KIEIKLSDIP EGKNMAFKWR GKPLFVRHRT 

       190        200        210        220        230        240 
QKEIEQEAAV ELSQLRDPQH DLDRVKKPEW VILIGVCTHL GCVPIANAGD FGGYYCPCHG 

       250        260        270 
SHYDASGRIR LGPAPLNLEV PTYEFTSDDM VIVG 

« Hide

References

« Hide 'large scale' references
[1]"The primary structure of human Rieske iron-sulfur protein of mitochondrial cytochrome bc1 complex deduced from cDNA analysis."
Nishikimi M., Hosokawa Y., Toda H., Suzuki H., Ozawa T.
Biochem. Int. 20:155-160(1990) [PubMed: 2158323] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structure, sequence and location of the UQCRFS1 gene for the human Rieske Fe-S protein."
Pennacchio L., Bergmann A., Fukushima A., Salemi A., Okubo K., Lennon G.
Gene 155:207-211(1995) [PubMed: 7721092] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed: 15057824] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain, Lung and Lymph.
[5]"Renal cell carcinoma and normal kidney protein expression."
Sarto C., Marocchi A., Sanchez J.-C., Giannone B., Frutiger S., Golaz O., Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F., Mocarelli P.
Electrophoresis 18:599-604(1997) [PubMed: 9150947] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE OF 79-90.
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

L32977, L32917 Genomic DNA. Translation: AAC41754.1.
AC007786 Genomic DNA. Translation: AAD38242.1.
BC000649 mRNA. Translation: AAH00649.1.
BC010035 mRNA. Translation: AAH10035.1.
BC067832 mRNA. Translation: AAH67832.1.
RefSeqNP_005994.2.
UniGeneHs.170107

3D structure databases

HSSPHSSP built from PDB template 1RIE based on UniProtKB P13272.
SMRP47985. Positions 1-57, 79-274.
ModBaseSearch...

Protein-protein interaction databases

IntActP47985.

PTM databases

PhosphoSiteP47985.

2-D gel databases

SWISS-2DPAGEP47985.
HSC-2DPAGEP47985.
OGPP47985.
REPRODUCTION-2DPAGEIPI00026964.

Genome annotation databases

EnsemblENSG00000169021. Homo sapiens. [Contig view]
GeneID7386.
KEGGhsa:7386.
NMPDRfig|9606.3.peg.16154.

Organism-specific databases

H-InvDBHIX0014973.
HIX0041192.
HIX0080950.
HGNCHGNC:12587. UQCRFS1.
MIM191327. gene.
PharmGKBPA37218.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMP47985.
HOVERGENP47985.

Enzyme and pathway databases

ReactomeREACT_6305. Electron Transport Chain.

Gene expression databases

ArrayExpressP47985.
CleanExHS_UQCRFS1.
GermOnlineENSG00000169021. Homo sapiens.

Family and domain databases

InterProIPR014349. Rieske.
IPR005805. Rieske_C.
IPR006317. Rieske_proteo.
IPR005806. Rieske_reg.
IPR015248. Ubiq_cytc_red_N.
IPR004192. UCR_TM_region.
[Graphical view]
Gene3DG3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHERPTHR10134. Rieske. 1 hit.
PfamPF00355. Rieske. 1 hit.
PF09165. Ubiq-Cytc-red_N. 1 hit.
PF02921. UCR_TM. 1 hit.
[Graphical view]
PRINTSPR00162. RIESKE.
TIGRFAMsTIGR01416. Rieske_proteo. 1 hit.
PROSITEPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

LinkHubP47985.
NextBio28920.
SOURCESearch...

Entry information

Entry nameUCRI_HUMAN
AccessionPrimary (citable) accession number: P47985
Secondary accession number(s): Q6NVX5, Q9UPH2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: April 3, 2007
Last modified: November 25, 2008
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents