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Protein

mRNA decay factor CTH2

Gene

TIS11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to specific AU-rich elements (ARE) in the 3'-untranslated region of target mRNAs and promotes their degradation. In response to iron deficiency, promotes the decay of many mRNAs encoding proteins involved in iron-dependent pathways. Recruits the DHH1 helicase to the SDH4 mRNA and promotes SDH4 mRNA decay. Also destabilizes target mRNA by modulating 3'-end processing, creating extended transcripts that are prone for degradation.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri169 – 197C3H1-type 1PROSITE-ProRule annotationAdd BLAST29
Zinc fingeri207 – 235C3H1-type 2PROSITE-ProRule annotationAdd BLAST29

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • mRNA 3'-UTR binding Source: GO_Central
  • mRNA binding Source: SGD

GO - Biological processi

  • 3'-UTR-mediated mRNA destabilization Source: GO_Central
  • cellular iron ion homeostasis Source: SGD
  • nuclear-transcribed mRNA catabolic process Source: SGD

Keywordsi

Molecular functionRNA-binding
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32276-MONOMER
ReactomeiR-SCE-450385 Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA decay factor CTH2
Alternative name(s):
Cysteine-three-histidine protein 2
Protein TIS11 homolog
Protein YTIS11
TPA-induced sequence protein 11
Gene namesi
Name:TIS11
Synonyms:CTH2
Ordered Locus Names:YLR136C
ORF Names:L3143, L9606.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR136C
SGDiS000004126 TIS11

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi190C → A or R: Abolishes mRNA binding. 1 Publication1
Mutagenesisi213C → A or R: Abolishes mRNA binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000891741 – 285mRNA decay factor CTH2Add BLAST285

Proteomic databases

PaxDbiP47977
PRIDEiP47977

Expressioni

Inductioni

By transcription factors AFT1 and AFT2 in response to iron deficiency.1 Publication

Interactioni

Subunit structurei

Interacts with DHH1.1 Publication

Protein-protein interaction databases

BioGridi31405, 59 interactors
DIPiDIP-5614N
IntActiP47977, 2 interactors
STRINGi4932.YLR136C

Structurei

3D structure databases

ProteinModelPortaliP47977
SMRiP47977
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 55Required for mRNA decay activityAdd BLAST19

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri169 – 197C3H1-type 1PROSITE-ProRule annotationAdd BLAST29
Zinc fingeri207 – 235C3H1-type 2PROSITE-ProRule annotationAdd BLAST29

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00530000063262
HOGENOMiHOG000001038
InParanoidiP47977
OrthoDBiEOG092C4AZJ

Family and domain databases

InterProiView protein in InterPro
IPR000571 Znf_CCCH
IPR036855 Znf_CCCH_sf
PfamiView protein in Pfam
PF00642 zf-CCCH, 2 hits
SMARTiView protein in SMART
SM00356 ZnF_C3H1, 2 hits
SUPFAMiSSF90229 SSF90229, 2 hits
PROSITEiView protein in PROSITE
PS50103 ZF_C3H1, 2 hits

Sequencei

Sequence statusi: Complete.

P47977-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWAQLSYTRP ESQKTDLTSL FSTDQEQNPL NDYQYQINIR ELEEYYNKTI
60 70 80 90 100
LNEDNIQETS SEISSAVSFS PPKNTNAIQP GLLYDPQLMN PFLPSAHLNS
110 120 130 140 150
TAPTTFKKKL EVQINPDYVP KSSQLPLTSQ NLQQLSQQKP KNDASFSSEK
160 170 180 190 200
ESSAQPKVKS QVQETPKQLY KTELCESFTL KGSCPYGSKC QFAHGLGELK
210 220 230 240 250
VKKSCKNFRT KPCVNWEKLG YCPYGRRCCF KHGDDNDIAV YVKAGTYCNV
260 270 280
SSTSKQSDEK RSNGRGSAKK KNLNVKVKAL QRMTW
Length:285
Mass (Da):32,314
Last modified:October 1, 1996 - v1
Checksum:i72E041AE31EC4099
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S76619 Genomic DNA Translation: AAB33266.1
L42134 Genomic DNA Translation: AAB39898.1
X91258 Genomic DNA Translation: CAA62651.1
Z73308 Genomic DNA Translation: CAA97707.1
U53881 Genomic DNA Translation: AAB82400.1
AY558210 Genomic DNA Translation: AAS56536.1
BK006945 Genomic DNA Translation: DAA09447.1
PIRiS59328
RefSeqiNP_013237.1, NM_001182023.1

Genome annotation databases

EnsemblFungiiYLR136C; YLR136C; YLR136C
GeneIDi850827
KEGGisce:YLR136C

Similar proteinsi

Entry informationi

Entry nameiCTH2_YEAST
AccessioniPrimary (citable) accession number: P47977
Secondary accession number(s): D6VYD1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health