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Protein

mRNA decay factor CTH2

Gene

TIS11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to specific AU-rich elements (ARE) in the 3'-untranslated region of target mRNAs and promotes their degradation. In response to iron deficiency, promotes the decay of many mRNAs encoding proteins involved in iron-dependent pathways. Recruits the DHH1 helicase to the SDH4 mRNA and promotes SDH4 mRNA decay. Also destabilizes target mRNA by modulating 3'-end processing, creating extended transcripts that are prone for degradation.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri169 – 197C3H1-type 1PROSITE-ProRule annotationAdd BLAST29
Zinc fingeri207 – 235C3H1-type 2PROSITE-ProRule annotationAdd BLAST29

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • mRNA 3'-UTR binding Source: GO_Central
  • mRNA binding Source: SGD

GO - Biological processi

  • 3'-UTR-mediated mRNA destabilization Source: GO_Central
  • cellular iron ion homeostasis Source: SGD
  • nuclear-transcribed mRNA catabolic process Source: SGD
Complete GO annotation...

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32276-MONOMER.
ReactomeiR-SCE-450385. Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA decay factor CTH2
Alternative name(s):
Cysteine-three-histidine protein 2
Protein TIS11 homolog
Protein YTIS11
TPA-induced sequence protein 11
Gene namesi
Name:TIS11
Synonyms:CTH2
Ordered Locus Names:YLR136C
ORF Names:L3143, L9606.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR136C.
SGDiS000004126. TIS11.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytoplasmic mRNA processing body Source: UniProtKB-SubCell
  • cytosol Source: GO_Central
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi190C → A or R: Abolishes mRNA binding. 1 Publication1
Mutagenesisi213C → A or R: Abolishes mRNA binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000891741 – 285mRNA decay factor CTH2Add BLAST285

Proteomic databases

PRIDEiP47977.

Expressioni

Inductioni

By transcription factors AFT1 and AFT2 in response to iron deficiency.1 Publication

Interactioni

Subunit structurei

Interacts with DHH1.1 Publication

Protein-protein interaction databases

BioGridi31405. 21 interactors.
DIPiDIP-5614N.
IntActiP47977. 1 interactor.
MINTiMINT-504800.

Structurei

3D structure databases

ProteinModelPortaliP47977.
SMRiP47977.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 55Required for mRNA decay activityAdd BLAST19

Sequence similaritiesi

Contains 2 C3H1-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri169 – 197C3H1-type 1PROSITE-ProRule annotationAdd BLAST29
Zinc fingeri207 – 235C3H1-type 2PROSITE-ProRule annotationAdd BLAST29

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00530000063262.
HOGENOMiHOG000001038.
InParanoidiP47977.
OMAiYCRYGKR.
OrthoDBiEOG092C4AZJ.

Family and domain databases

Gene3Di4.10.1000.10. 2 hits.
InterProiIPR000571. Znf_CCCH.
[Graphical view]
PfamiPF00642. zf-CCCH. 2 hits.
[Graphical view]
SMARTiSM00356. ZnF_C3H1. 2 hits.
[Graphical view]
SUPFAMiSSF90229. SSF90229. 2 hits.
PROSITEiPS50103. ZF_C3H1. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47977-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWAQLSYTRP ESQKTDLTSL FSTDQEQNPL NDYQYQINIR ELEEYYNKTI
60 70 80 90 100
LNEDNIQETS SEISSAVSFS PPKNTNAIQP GLLYDPQLMN PFLPSAHLNS
110 120 130 140 150
TAPTTFKKKL EVQINPDYVP KSSQLPLTSQ NLQQLSQQKP KNDASFSSEK
160 170 180 190 200
ESSAQPKVKS QVQETPKQLY KTELCESFTL KGSCPYGSKC QFAHGLGELK
210 220 230 240 250
VKKSCKNFRT KPCVNWEKLG YCPYGRRCCF KHGDDNDIAV YVKAGTYCNV
260 270 280
SSTSKQSDEK RSNGRGSAKK KNLNVKVKAL QRMTW
Length:285
Mass (Da):32,314
Last modified:October 1, 1996 - v1
Checksum:i72E041AE31EC4099
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S76619 Genomic DNA. Translation: AAB33266.1.
L42134 Genomic DNA. Translation: AAB39898.1.
X91258 Genomic DNA. Translation: CAA62651.1.
Z73308 Genomic DNA. Translation: CAA97707.1.
U53881 Genomic DNA. Translation: AAB82400.1.
AY558210 Genomic DNA. Translation: AAS56536.1.
BK006945 Genomic DNA. Translation: DAA09447.1.
PIRiS59328.
RefSeqiNP_013237.1. NM_001182023.1.

Genome annotation databases

EnsemblFungiiYLR136C; YLR136C; YLR136C.
GeneIDi850827.
KEGGisce:YLR136C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S76619 Genomic DNA. Translation: AAB33266.1.
L42134 Genomic DNA. Translation: AAB39898.1.
X91258 Genomic DNA. Translation: CAA62651.1.
Z73308 Genomic DNA. Translation: CAA97707.1.
U53881 Genomic DNA. Translation: AAB82400.1.
AY558210 Genomic DNA. Translation: AAS56536.1.
BK006945 Genomic DNA. Translation: DAA09447.1.
PIRiS59328.
RefSeqiNP_013237.1. NM_001182023.1.

3D structure databases

ProteinModelPortaliP47977.
SMRiP47977.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31405. 21 interactors.
DIPiDIP-5614N.
IntActiP47977. 1 interactor.
MINTiMINT-504800.

Proteomic databases

PRIDEiP47977.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR136C; YLR136C; YLR136C.
GeneIDi850827.
KEGGisce:YLR136C.

Organism-specific databases

EuPathDBiFungiDB:YLR136C.
SGDiS000004126. TIS11.

Phylogenomic databases

GeneTreeiENSGT00530000063262.
HOGENOMiHOG000001038.
InParanoidiP47977.
OMAiYCRYGKR.
OrthoDBiEOG092C4AZJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-32276-MONOMER.
ReactomeiR-SCE-450385. Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA.

Miscellaneous databases

PROiP47977.

Family and domain databases

Gene3Di4.10.1000.10. 2 hits.
InterProiIPR000571. Znf_CCCH.
[Graphical view]
PfamiPF00642. zf-CCCH. 2 hits.
[Graphical view]
SMARTiSM00356. ZnF_C3H1. 2 hits.
[Graphical view]
SUPFAMiSSF90229. SSF90229. 2 hits.
PROSITEiPS50103. ZF_C3H1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCTH2_YEAST
AccessioniPrimary (citable) accession number: P47977
Secondary accession number(s): D6VYD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.