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Protein

mRNA decay factor CTH2

Gene

TIS11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to specific AU-rich elements (ARE) in the 3'-untranslated region of target mRNAs and promotes their degradation. In response to iron deficiency, promotes the decay of many mRNAs encoding proteins involved in iron-dependent pathways. Recruits the DHH1 helicase to the SDH4 mRNA and promotes SDH4 mRNA decay. Also destabilizes target mRNA by modulating 3'-end processing, creating extended transcripts that are prone for degradation.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri169 – 19729C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri207 – 23529C3H1-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • mRNA binding Source: SGD

GO - Biological processi

  • cellular iron ion homeostasis Source: SGD
  • nuclear-transcribed mRNA catabolic process Source: SGD
Complete GO annotation...

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32276-MONOMER.
ReactomeiR-SCE-450385. Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA decay factor CTH2
Alternative name(s):
Cysteine-three-histidine protein 2
Protein TIS11 homolog
Protein YTIS11
TPA-induced sequence protein 11
Gene namesi
Name:TIS11
Synonyms:CTH2
Ordered Locus Names:YLR136C
ORF Names:L3143, L9606.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR136C.
SGDiS000004126. TIS11.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytoplasmic mRNA processing body Source: UniProtKB-SubCell
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi190 – 1901C → A or R: Abolishes mRNA binding. 1 Publication
Mutagenesisi213 – 2131C → A or R: Abolishes mRNA binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285mRNA decay factor CTH2PRO_0000089174Add
BLAST

Expressioni

Inductioni

By transcription factors AFT1 and AFT2 in response to iron deficiency.1 Publication

Interactioni

Subunit structurei

Interacts with DHH1.1 Publication

Protein-protein interaction databases

BioGridi31405. 21 interactions.
DIPiDIP-5614N.
IntActiP47977. 1 interaction.
MINTiMINT-504800.

Structurei

3D structure databases

ProteinModelPortaliP47977.
SMRiP47977. Positions 165-235.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni37 – 5519Required for mRNA decay activityAdd
BLAST

Sequence similaritiesi

Contains 2 C3H1-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri169 – 19729C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri207 – 23529C3H1-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00530000063262.
HOGENOMiHOG000001038.
InParanoidiP47977.
OrthoDBiEOG7ZD261.

Family and domain databases

Gene3Di4.10.1000.10. 2 hits.
InterProiIPR000571. Znf_CCCH.
[Graphical view]
PfamiPF00642. zf-CCCH. 2 hits.
[Graphical view]
SMARTiSM00356. ZnF_C3H1. 2 hits.
[Graphical view]
SUPFAMiSSF90229. SSF90229. 2 hits.
PROSITEiPS50103. ZF_C3H1. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47977-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWAQLSYTRP ESQKTDLTSL FSTDQEQNPL NDYQYQINIR ELEEYYNKTI
60 70 80 90 100
LNEDNIQETS SEISSAVSFS PPKNTNAIQP GLLYDPQLMN PFLPSAHLNS
110 120 130 140 150
TAPTTFKKKL EVQINPDYVP KSSQLPLTSQ NLQQLSQQKP KNDASFSSEK
160 170 180 190 200
ESSAQPKVKS QVQETPKQLY KTELCESFTL KGSCPYGSKC QFAHGLGELK
210 220 230 240 250
VKKSCKNFRT KPCVNWEKLG YCPYGRRCCF KHGDDNDIAV YVKAGTYCNV
260 270 280
SSTSKQSDEK RSNGRGSAKK KNLNVKVKAL QRMTW
Length:285
Mass (Da):32,314
Last modified:October 1, 1996 - v1
Checksum:i72E041AE31EC4099
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S76619 Genomic DNA. Translation: AAB33266.1.
L42134 Genomic DNA. Translation: AAB39898.1.
X91258 Genomic DNA. Translation: CAA62651.1.
Z73308 Genomic DNA. Translation: CAA97707.1.
U53881 Genomic DNA. Translation: AAB82400.1.
AY558210 Genomic DNA. Translation: AAS56536.1.
BK006945 Genomic DNA. Translation: DAA09447.1.
PIRiS59328.
RefSeqiNP_013237.1. NM_001182023.1.

Genome annotation databases

EnsemblFungiiYLR136C; YLR136C; YLR136C.
GeneIDi850827.
KEGGisce:YLR136C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S76619 Genomic DNA. Translation: AAB33266.1.
L42134 Genomic DNA. Translation: AAB39898.1.
X91258 Genomic DNA. Translation: CAA62651.1.
Z73308 Genomic DNA. Translation: CAA97707.1.
U53881 Genomic DNA. Translation: AAB82400.1.
AY558210 Genomic DNA. Translation: AAS56536.1.
BK006945 Genomic DNA. Translation: DAA09447.1.
PIRiS59328.
RefSeqiNP_013237.1. NM_001182023.1.

3D structure databases

ProteinModelPortaliP47977.
SMRiP47977. Positions 165-235.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31405. 21 interactions.
DIPiDIP-5614N.
IntActiP47977. 1 interaction.
MINTiMINT-504800.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR136C; YLR136C; YLR136C.
GeneIDi850827.
KEGGisce:YLR136C.

Organism-specific databases

EuPathDBiFungiDB:YLR136C.
SGDiS000004126. TIS11.

Phylogenomic databases

GeneTreeiENSGT00530000063262.
HOGENOMiHOG000001038.
InParanoidiP47977.
OrthoDBiEOG7ZD261.

Enzyme and pathway databases

BioCyciYEAST:G3O-32276-MONOMER.
ReactomeiR-SCE-450385. Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA.

Miscellaneous databases

NextBioi967088.
PROiP47977.

Family and domain databases

Gene3Di4.10.1000.10. 2 hits.
InterProiIPR000571. Znf_CCCH.
[Graphical view]
PfamiPF00642. zf-CCCH. 2 hits.
[Graphical view]
SMARTiSM00356. ZnF_C3H1. 2 hits.
[Graphical view]
SUPFAMiSSF90229. SSF90229. 2 hits.
PROSITEiPS50103. ZF_C3H1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast homologue YTIS11, of the mammalian TIS11 gene family is a non-essential, glucose repressible gene."
    Ma Q., Herschman H.R.
    Oncogene 10:487-494(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning and characterization of two yeast genes encoding members of the CCCH class of zinc finger proteins: zinc finger-mediated impairment of cell growth."
    Thompson M.J., Lai W.S., Taylor G.A., Blackshear P.J.
    Gene 174:225-233(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. "Coordinated remodeling of cellular metabolism during iron deficiency through targeted mRNA degradation."
    Puig S., Askeland E., Thiele D.J.
    Cell 120:99-110(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, MUTAGENESIS OF CYS-190 AND CYS-213, MRNA-BINDING.
  8. "Cooperation of two mRNA-binding proteins drives metabolic adaptation to iron deficiency."
    Puig S., Vergara S.V., Thiele D.J.
    Cell Metab. 7:555-564(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Regulation of ARE transcript 3' end processing by the yeast Cth2 mRNA decay factor."
    Prouteau M., Daugeron M.-C., Seraphin B.
    EMBO J. 27:2966-2976(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  10. "The Cth2 ARE-binding protein recruits the Dhh1 helicase to promote the decay of succinate dehydrogenase SDH4 mRNA in response to iron deficiency."
    Pedro-Segura E., Vergara S.V., Rodriguez-Navarro S., Parker R., Thiele D.J., Puig S.
    J. Biol. Chem. 283:28527-28535(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH DHH1, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCTH2_YEAST
AccessioniPrimary (citable) accession number: P47977
Secondary accession number(s): D6VYD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.