P47974 (TISD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 36, C3H1 type-like 2

Alternative name(s):
Butyrate response factor 2
EGF-response factor 2
Short name=ERF-2
Protein TIS11D

Gene names

Name:	ZFP36L2
Synonyms:	BRF2, ERF2, RNF162C, TIS11D

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable regulatory protein involved in regulating the response to growth factors. RNA-binding protein that binds to 5'UUAUUUAUUU-3' core sequence. Binds to the class II AU-rich element (ARE) in the 3'-UTR of target mRNAs and promotes their deadenylation and degradation.
Subcellular location	Nucleus Potential.
Sequence similarities	Contains 2 C3H1-type zinc fingers.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding RNA-binding Zinc
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell proliferation Traceable author statement. Source: ProtInc
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Traceable author statement. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 494

494

Zinc finger protein 36, C3H1 type-like 2

PRO_0000089170

Regions

Zinc finger

153 – 181

29

C3H1-type 1

Zinc finger

191 – 219

29

C3H1-type 2

Region

170 – 211

42

RNA-binding

Motif

153 – 158

6

RNA-binding

Compositional bias

106 – 109

4

Poly-Gly

Compositional bias

138 – 141

4

Poly-Gln

Compositional bias

143 – 146

4

Poly-Gly

Compositional bias

288 – 291

4

Poly-Pro

Compositional bias

323 – 332

10

Poly-Ala

Compositional bias

384 – 390

7

Poly-Ala

Compositional bias

395 – 401

7

Poly-Gln

Experimental info

Sequence conflict

96 – 97

2

TS → DL in AAA91778. Ref.1

Sequence conflict

318

1

A → T in CAA55592. Ref.2

Sequence conflict

329 – 332

4

AAAA → LR in CAA55592. Ref.2

Sequence conflict

331 – 332

2

Missing in AAA91778. Ref.1

Sequence conflict

400

1

Q → QQQQ in AAH05010. Ref.5

Sequence conflict

453 – 462

10

Missing in AAA91778. Ref.1

Secondary structure

1

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.

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.

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494

Helix Strand Turn

Details...

Turn

152 – 155

4

Beta strand

156 – 158

3

Helix

160 – 165

6

Helix

171 – 173

3

Beta strand

175 – 179

5

Helix

180 – 182

3

Turn

190 – 193

4

Helix

198 – 203

6

Helix

209 – 211

3

Beta strand

213 – 215

3

Sequences

	Sequence		Length	Mass (Da)
	P47974 [UniParc]. Last modified May 1, 2007. Version 3. Checksum: 10E23FA9E2DDABD4	FASTA	494	51,063
	10 20 30 40 50 60 MSTTLLSAFY DVDFLCKTEK SLANLNLNNM LDKKAVGTPV AAAPSSGFAP GFLRRHSASN 70 80 90 100 110 120 LHALAHPAPS PGSCSPKFPG AANGSSCGSA AAGGPTSYGT LKEPSGGGGT ALLNKENKFR 130 140 150 160 170 180 DRSFSENGDR SQHLLHLQQQ QKGGGGSQIN STRYKTELCR PFEESGTCKY GEKCQFAHGF 190 200 210 220 230 240 HELRSLTRHP KYKTELCRTF HTIGFCPYGP RCHFIHNADE RRPAPSGGAS GDLRAFGTRD 250 260 270 280 290 300 ALHLGFPREP RPKLHHSLSF SGFPSGHHQP PGGLESPLLL DSPTSRTPPP PSCSSASSCS 310 320 330 340 350 360 SSASSCSSAS AASTPSGAPT CCASAAAAAA AALLYGTGGA EDLLAPGAPC AACSSASCAN 370 380 390 400 410 420 NAFAFGPELS SLITPLAIQT HNFAAVAAAA YYRSQQQQQQ QGLAPPAQPP APPSATLPAG 430 440 450 460 470 480 AAAPPSPPFS FQLPRRLSDS PVFDAPPSPP DSLSDRDSYL SGSLSSGSLS GSESPSLDPG 490 RRLPIFSRLS ISDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a member of the TIS11 early response gene family at the insertion point of a DNA fragment containing a gene for the T-cell receptor beta chain in an acute T-cell leukemia." Ino T., Yasui H., Hirano M., Kurosawa Y. Oncogene 11:2705-2710(1995) [PubMed: 8545129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"ERF-2, the human homologue of the murine Tis11d early response gene." Nie X.F., Maclean K.N., Kumar V., McKay I.A., Bustin S.A. Gene 152:285-286(1995) [PubMed: 7835719] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
[6]	"Recognition of the mRNA AU-rich element by the zinc finger domain of TIS11d." Hudson B.P., Martinez-Yamout M.A., Dyson H.J., Wright P.E. Nat. Struct. Mol. Biol. 11:257-264(2004) [PubMed: 14981510] [Abstract] Cited for: STRUCTURE BY NMR OF 151-220 IN COMPLEX WITH RNA.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U07802 Genomic DNA. Translation: AAA91778.1.
X78992 mRNA. Translation: CAA55592.1.
AC010883 Genomic DNA. Translation: AAY14992.1.
CH471053 Genomic DNA. Translation: EAX00306.1.
BC005010 mRNA. Translation: AAH05010.1.

IPI

IPI00604786.

PIR

S49147.

RefSeq

NP_008818.3. NM_006887.4.

UniGene

Hs.503093.
Hs.645068.
Hs.705738.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RGO	NMR	-	A	151-220	[»]

ProteinModelPortal

P47974.

SMR

P47974. Positions 151-220.

ModBase

Search...

Protein-protein interaction databases

IntAct

P47974. 2 interactions.

STRING

P47974.

PTM databases

PhosphoSite

P47974.

Polymorphism databases

DMDM

146291085.

Proteomic databases

PRIDE

P47974.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000282388; ENSP00000282388; ENSG00000152518.

GeneID

678.

KEGG

hsa:678.

UCSC

uc002rsv.2. human.

Organism-specific databases

CTD

678.

GeneCards

GC02M043449.

H-InvDB

HIX0002007.

HGNC

HGNC:1108. ZFP36L2.

HPA

HPA047428.

neXtProt

NX_P47974.

PharmGKB

PA35028.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG19833.

GeneTree

ENSGT00530000063262.

HOGENOM

HBG717837.

HOVERGEN

HBG008483.

InParanoid

P47974.

OMA

IQTHNFA.

OrthoDB

EOG480HXD.

PhylomeDB

P47974.

Gene expression databases

ArrayExpress

P47974.

Bgee

P47974.

CleanEx

HS_BRF2.
HS_ZFP36L2.

Genevestigator

P47974.

GermOnline

ENSG00000152518. Homo sapiens.

Family and domain databases

InterPro

IPR007635. Tis11B_N.
IPR000571. Znf_CCCH.
[Graphical view]

Pfam

PF04553. Tis11B_N. 1 hit.
PF00642. zf-CCCH. 2 hits.
[Graphical view]

SMART

SM00356. ZnF_C3H1. 2 hits.
[Graphical view]

PROSITE

PS50103. ZF_C3H1. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

2794.

Entry information

Entry name

TISD_HUMAN

Accession

Primary (citable) accession number: P47974
Secondary accession number(s): Q53TB4, Q9BSJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 1, 2007
Last modified:	January 25, 2012
This is version 108 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.